taq polymerase  (Qiagen)

 
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    Name:
    Taq DNA Polymerase
    Description:
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    Catalog Number:
    201203
    Price:
    134
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    Structured Review

    Qiagen taq polymerase
    Melting curves of probes S8807 and S8807A. The melting behavior of two TaqMan probes hybridizing to template DNAs from Synechococcus spp. strains BO 8807 and BO 8808 was analyzed by end point TNA. <t>PCR</t> conditions: 1 ng of template DNA, 50 nM probe (S8807 or S8807A), 2.5 mM Mg 2+ , 0.625 U of <t>Taq</t> polymerase, 50 nM primers PITSANF and PITSEND at annealing-extension temperatures of 56 to 65°C, 200 nM at 66 and 67°C, 400 nM at 68 and 69°C, and 800 nM at 70°C. Cycling conditions: 1.5 min of annealing-polymerization, 30 cycles.
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    https://www.bioz.com/result/taq polymerase/product/Qiagen
    Average 99 stars, based on 867 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities †"

    Article Title: PCR Bias in Ecological Analysis: a Case Study for Quantitative Taq Nuclease Assays in Analyses of Microbial Communities †

    Journal: Applied and Environmental Microbiology

    doi:

    Melting curves of probes S8807 and S8807A. The melting behavior of two TaqMan probes hybridizing to template DNAs from Synechococcus spp. strains BO 8807 and BO 8808 was analyzed by end point TNA. PCR conditions: 1 ng of template DNA, 50 nM probe (S8807 or S8807A), 2.5 mM Mg 2+ , 0.625 U of Taq polymerase, 50 nM primers PITSANF and PITSEND at annealing-extension temperatures of 56 to 65°C, 200 nM at 66 and 67°C, 400 nM at 68 and 69°C, and 800 nM at 70°C. Cycling conditions: 1.5 min of annealing-polymerization, 30 cycles.
    Figure Legend Snippet: Melting curves of probes S8807 and S8807A. The melting behavior of two TaqMan probes hybridizing to template DNAs from Synechococcus spp. strains BO 8807 and BO 8808 was analyzed by end point TNA. PCR conditions: 1 ng of template DNA, 50 nM probe (S8807 or S8807A), 2.5 mM Mg 2+ , 0.625 U of Taq polymerase, 50 nM primers PITSANF and PITSEND at annealing-extension temperatures of 56 to 65°C, 200 nM at 66 and 67°C, 400 nM at 68 and 69°C, and 800 nM at 70°C. Cycling conditions: 1.5 min of annealing-polymerization, 30 cycles.

    Techniques Used: Polymerase Chain Reaction

    Quantification of DNA from Synechococcus sp. strain BO 8807 by end point TNA. Twenty-five-microliter Taq nuclease assay mixtures contained 10 −3 to 10 3 ng of DNA, representing approximately 3 × 10 2 to 3 × 10 8 genome copies of Synechococcus sp. strain BO 8807; 50 nM primers PITSANF and PITSEND; 50 nM probe S8807A; 2.5 mM Mg 2+ ; and 0.625 U of Taq polymerase. PCR conditions: annealing-polymerization at 60°C for 1.5 min, 30 cycles. The insert shows a 1% agarose gel containing 2 μl of each assay mixture per lane. PCR products were stained with ethidium bromide. Lanes M, λ DNA digested with Pst I.
    Figure Legend Snippet: Quantification of DNA from Synechococcus sp. strain BO 8807 by end point TNA. Twenty-five-microliter Taq nuclease assay mixtures contained 10 −3 to 10 3 ng of DNA, representing approximately 3 × 10 2 to 3 × 10 8 genome copies of Synechococcus sp. strain BO 8807; 50 nM primers PITSANF and PITSEND; 50 nM probe S8807A; 2.5 mM Mg 2+ ; and 0.625 U of Taq polymerase. PCR conditions: annealing-polymerization at 60°C for 1.5 min, 30 cycles. The insert shows a 1% agarose gel containing 2 μl of each assay mixture per lane. PCR products were stained with ethidium bromide. Lanes M, λ DNA digested with Pst I.

    Techniques Used: Nuclease Assay, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Related Articles

    Multiplexing:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Multiplex Assay:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Southern Blot:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    Concentration Assay:

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

    Incubation:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    other:

    Article Title: Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction
    Article Snippet: The results showed a tendency that positive signals were improved and background signals were decreased compared to the use of HotStar Taq DNA polymerase, although statistical evidence is lacking (results not shown).

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: TNAs of wt protoplasts cotransfected with pTOM6 together with pTOM100C4(−) or pTOM100NT were treated with Taq DNA polymerase for increasing times (Fig. A).

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: A fraction of viral DNA from pTOM6-pTOM100NT-cotransfected protoplasts also reacted with Taq DNA polymerase, generating ocDNA.

    Polymerase Chain Reaction:

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

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  • 99
    Qiagen taq dna polymerase
    Slowly migrating DNAs are converted into ocDNA by <t>Taq</t> (A) or T4 (B) <t>DNA</t> polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.
    Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Qiagen
    Average 99 stars, based on 1118 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Slowly migrating DNAs are converted into ocDNA by Taq (A) or T4 (B) DNA polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.

    Journal: Journal of Virology

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication

    doi: 10.1128/JVI.75.22.10573-10581.2001

    Figure Lengend Snippet: Slowly migrating DNAs are converted into ocDNA by Taq (A) or T4 (B) DNA polymerase treatment. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slowly migrating viral DNAs are indicated with an asterisk (∗). (A) TNAs were extracted from wt protoplasts at 72 h posttransfection with pTOM6 alone (the two lanes on the right) or together with pTOM100C4(−) or pTOM100NT and analyzed directly (−) or following incubation with Taq DNA polymerase for the time indicated below. (B) TNAs were extracted from wt or transgenic (102.22) protoplasts at 72 h posttransfection with pSP97 (TYLCSV-ES[1]) and analyzed following a 1-h incubation with (+) or without (−) T4 DNA polymerase. Lane C, TNAs from a TYLCSV-infected tomato plant digested with Bgl II to show migration of linear DNA.

    Article Snippet: To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B).

    Techniques: Incubation, Transgenic Assay, Infection, Migration

    Slowly migrating DNAs also accumulate at early stages after transfection of wt protoplasts with TYLCSV. TNAs were extracted from protoplasts transfected with pTOM6. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slow-migrating viral DNAs are indicated with an asterisk (∗). HPT, hours posttransfection. (A) Time course analysis of viral DNA forms. Sample at 72 h was diluted 20-fold to give signals of satisfactory intensity on the autoradiographic film. (B) Taq DNA polymerase treatment of TNAs at 24 and 72 h after transfection. Samples were incubated for 10 min at 37°C with (+) or without (−) the polymerase. Lane C, sample at 72 h digested with Bgl II to show the linear DNA.

    Journal: Journal of Virology

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication

    doi: 10.1128/JVI.75.22.10573-10581.2001

    Figure Lengend Snippet: Slowly migrating DNAs also accumulate at early stages after transfection of wt protoplasts with TYLCSV. TNAs were extracted from protoplasts transfected with pTOM6. The blots were hybridized with a C1-sense RNA probe. The positions of ocDNA, linear DNA (linDNA), scDNA, and cssDNA forms of viral DNA are indicated. Slow-migrating viral DNAs are indicated with an asterisk (∗). HPT, hours posttransfection. (A) Time course analysis of viral DNA forms. Sample at 72 h was diluted 20-fold to give signals of satisfactory intensity on the autoradiographic film. (B) Taq DNA polymerase treatment of TNAs at 24 and 72 h after transfection. Samples were incubated for 10 min at 37°C with (+) or without (−) the polymerase. Lane C, sample at 72 h digested with Bgl II to show the linear DNA.

    Article Snippet: To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B).

    Techniques: Transfection, Incubation

    Melting temperature curve profiles produced by male and female samples in a HRM analysis. HRM analysis starts with a PCR amplification of the amelogenin gen. The amplification is in the presence of EvaGreen a saturating dye which presents high fluorescence when bound to dsDNA. Amplification is followed by a High Resolution Melting analysis (HRM) in the LightCycler 480 Real-Time PCR Instrument (Roche Applied Science). A dissociation curve analysis displays the different melting temperature of products from the X and Y chromosome.

    Journal: PLoS ONE

    Article Title: Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis

    doi: 10.1371/journal.pone.0104629

    Figure Lengend Snippet: Melting temperature curve profiles produced by male and female samples in a HRM analysis. HRM analysis starts with a PCR amplification of the amelogenin gen. The amplification is in the presence of EvaGreen a saturating dye which presents high fluorescence when bound to dsDNA. Amplification is followed by a High Resolution Melting analysis (HRM) in the LightCycler 480 Real-Time PCR Instrument (Roche Applied Science). A dissociation curve analysis displays the different melting temperature of products from the X and Y chromosome.

    Article Snippet: Lightcycler Master-Mix [composed by FastStart Taq DNA Polymerase, reaction buffer, dNTP mix (with dUTP instead of dTTP) and High Resolution Melting Dye] and nuclease-free water (QIAGEN), and 0.2 µM of each of the two primers: Amel_F ( 5′-CCCTTTGAAGTGGTACCAGAG-3′ ) and Amel_R (5′GGGAATAARGAACAAAATGTCTAC-3′, R = A or G).

    Techniques: Produced, Polymerase Chain Reaction, Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Journal: Indian Journal of Microbiology

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid

    doi: 10.1007/s12088-013-0431-y

    Figure Lengend Snippet: Sensitivity limits of multiplex RT-PCR. Lane 1 Amplification from tenfold diluted cDNA; lanes 2–6 amplicons from 10 −2 to 10 −6 fold diluted cDNA. Lanes 1–6 amplicons obtained with multiplexing kit; lanes 7–12 amplification with hot-start Taq DNA polymerase at same dilutions; lane M 100 bp marker

    Article Snippet: The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control.

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Multiplexing, Marker

    Examples of AS-PCR products for kdr -e and kdr -w genotyping . Gels A to D show examples of AS-PCR results using four different protocols, A [29], B [11], C [12], D [20]. Gels E to G show the result of using different DNA polymerases on the AS-PCR method described by [20], E: Promega PCR master mix, F: Qiagen HotStar Taq G:Finzymes Dynazyme II Gel H is an example of results of using the protocol of [20] with the Promega PCR master mix to genotype samples using the kdr -e assay from the 96 reference plate and gel I for the kdr -w assay. The same DNA templates were used in PCRs shown in gels A to G and from left to right were 100 bp DNA Ladder (Fermentas), homozygous wildtype, homozygous wildtype, heterozygous, heterozygous, homozygous mutant, homozygous mutant.

    Journal: Malaria Journal

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods

    doi: 10.1186/1475-2875-6-111

    Figure Lengend Snippet: Examples of AS-PCR products for kdr -e and kdr -w genotyping . Gels A to D show examples of AS-PCR results using four different protocols, A [29], B [11], C [12], D [20]. Gels E to G show the result of using different DNA polymerases on the AS-PCR method described by [20], E: Promega PCR master mix, F: Qiagen HotStar Taq G:Finzymes Dynazyme II Gel H is an example of results of using the protocol of [20] with the Promega PCR master mix to genotype samples using the kdr -e assay from the 96 reference plate and gel I for the kdr -w assay. The same DNA templates were used in PCRs shown in gels A to G and from left to right were 100 bp DNA Ladder (Fermentas), homozygous wildtype, homozygous wildtype, heterozygous, heterozygous, homozygous mutant, homozygous mutant.

    Article Snippet: PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen).

    Techniques: Polymerase Chain Reaction, Mutagenesis