taq polymerase buffer  (Thermo Fisher)


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    Name:
    Taq Buffer
    Description:
    Thermo Scientific 10X Taq Buffers are ready to use buffers for PCR using Taq DNA Polymerases both recombinant and native Available in different compositions and also without detergents 10X Taq Buffer with NH4 2SO4 B33 includes • 750 mM Tris HCl pH 8 8 at 25°C • 200 mM NH4 2SO4• 0 1 v v Tween 20Related ProductsTaq Buffer 10X with KCl and 15 mM MgCl2Taq Buffer 10X with KClTaq Buffer 10X with NH4 2SO4 and 20 mM MgCl2Taq Buffer 10X without detergent
    Catalog Number:
    b33
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Routine PCR
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher taq polymerase buffer
    <t>Primase</t> vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and <t>Taq</t> polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.
    Thermo Scientific 10X Taq Buffers are ready to use buffers for PCR using Taq DNA Polymerases both recombinant and native Available in different compositions and also without detergents 10X Taq Buffer with NH4 2SO4 B33 includes • 750 mM Tris HCl pH 8 8 at 25°C • 200 mM NH4 2SO4• 0 1 v v Tween 20Related ProductsTaq Buffer 10X with KCl and 15 mM MgCl2Taq Buffer 10X with KClTaq Buffer 10X with NH4 2SO4 and 20 mM MgCl2Taq Buffer 10X without detergent
    https://www.bioz.com/result/taq polymerase buffer/product/Thermo Fisher
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    taq polymerase buffer - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2"

    Article Title: A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkt1385

    Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.
    Figure Legend Snippet: Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.

    Techniques Used: Activity Assay, Incubation, Synthesized, SYBR Green Assay, Fluorescence, Standard Deviation

    Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.
    Figure Legend Snippet: Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.

    Techniques Used: DNA Synthesis, Incubation, SYBR Green Assay, Fluorescence, Standard Deviation

    2) Product Images from "Mutations in the MDR3 gene cause progressive familial intrahepatic cholestasis"

    Article Title: Mutations in the MDR3 gene cause progressive familial intrahepatic cholestasis

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Genomic DNA analysis that confirms the homozygosity of both patients and heterozygosity of their parents. ( A ) Taq I restriction analysis of amplified genomic DNA of patient 2 (A.B.) and his parents. In normal human genomic DNA the Taq I digestion of the 131-bp PCR fragment results in two fragments with a length of 75 and 56 bp. In patient 2, the homozygous mutation destroys this restriction site and no fragments are observed. In his heterozygous parents, a combination of the two cleaved fragments and the intact PCR product is observed. ( B ) PCR–SSCP analysis of genomic DNA, containing the 7-bp mutation, of patient 1 (B.K.) and his family. Denaturated DNA was loaded and electrophoresed in a 15% acrylamide/ N, N ′-methylenebisacrylamide (50:1, wt/wt) gel at 4°C. Patient 1 (lane 1), father (lane 2), mother (lane 3), sister (lane 4), and control (lane 5). In patient 1, only the deleted forms (sense and antisense) are observed. Both parents show the wild-type and the deleted forms, indicating that they are heterozygotes. The patient’s sister is homozygous for the wild-type form.
    Figure Legend Snippet: Genomic DNA analysis that confirms the homozygosity of both patients and heterozygosity of their parents. ( A ) Taq I restriction analysis of amplified genomic DNA of patient 2 (A.B.) and his parents. In normal human genomic DNA the Taq I digestion of the 131-bp PCR fragment results in two fragments with a length of 75 and 56 bp. In patient 2, the homozygous mutation destroys this restriction site and no fragments are observed. In his heterozygous parents, a combination of the two cleaved fragments and the intact PCR product is observed. ( B ) PCR–SSCP analysis of genomic DNA, containing the 7-bp mutation, of patient 1 (B.K.) and his family. Denaturated DNA was loaded and electrophoresed in a 15% acrylamide/ N, N ′-methylenebisacrylamide (50:1, wt/wt) gel at 4°C. Patient 1 (lane 1), father (lane 2), mother (lane 3), sister (lane 4), and control (lane 5). In patient 1, only the deleted forms (sense and antisense) are observed. Both parents show the wild-type and the deleted forms, indicating that they are heterozygotes. The patient’s sister is homozygous for the wild-type form.

    Techniques Used: Amplification, Polymerase Chain Reaction, Mutagenesis

    3) Product Images from "Detection and Identification of Leishmania DNA within Naturally Infected Sand Flies by Seminested PCR on Minicircle Kinetoplastic DNA"

    Article Title: Detection and Identification of Leishmania DNA within Naturally Infected Sand Flies by Seminested PCR on Minicircle Kinetoplastic DNA

    Journal: Applied and Environmental Microbiology

    doi:

    Sensitivity comparison between standard PCR with primers LINR4 and LIN17 or LINR4 and LIN19 (A) and seminested PCR with the set of primers LINR4, LIN17, and LIN19 (B). The DNA size marker (lane M) is PUC19 Taq I/PUC19 Sau 3A.
    Figure Legend Snippet: Sensitivity comparison between standard PCR with primers LINR4 and LIN17 or LINR4 and LIN19 (A) and seminested PCR with the set of primers LINR4, LIN17, and LIN19 (B). The DNA size marker (lane M) is PUC19 Taq I/PUC19 Sau 3A.

    Techniques Used: Polymerase Chain Reaction, Marker

    Specificity of the seminested PCR assay with primers LINR4, LIN17, and LIN19. The DNA size marker (lane M) is PUC19 Taq I/PUC19 Sau 3A.
    Figure Legend Snippet: Specificity of the seminested PCR assay with primers LINR4, LIN17, and LIN19. The DNA size marker (lane M) is PUC19 Taq I/PUC19 Sau 3A.

    Techniques Used: Polymerase Chain Reaction, Marker

    Related Articles

    Multiplex Assay:

    Article Title: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
    Article Snippet: .. Multiplex conventional PCR (cPCR) Conventional PCR assays were carried out in a final volume of 50 μl, containing: 5 μl DNA (20–25 ng), 5 μl 10× Taq Platinum buffer, 0.2 mM dNTPs, 4.5 mM MgCl2 , 1.25 U Taq Platinum DNA polymerase (Life Technologies, Carlsbad, CA, USA), 200 nM 121/122 primers (T. cruzi kDNA) [ , ] and 100 nM P2B/P6R primers (triatomine 12S rRNA gene). ..

    Amplification:

    Article Title: Rod Photoreceptor Ribbon Synapses in DBA/2J Mice Show Progressive Age-Related Structural Changes
    Article Snippet: .. PCR amplification was performed using 1 µl cDNA as a template in 10 µl PCR buffer (20 mM Tris–HCl pH 8.0, 50 mM KCl, 2.5 mM MgCl2 , 0.2 mM dNTPs, 2.5 mM, 1 U Taq-polymerase; Invitrogen) in a programmable thermocycler (GeneAmp PCR System 9700; Applied Biosystems, Foster City, CA) using primer pairs specific for C1qA (sense: 5′-agctgctggcatccggac-3′, antisense: 5′-ggtcccacttggagatcac-3′), C1qB (sense: 5′-cctgaggaccatcaacagc-3′, antisense: 5′-ctcctcttgctctagcttc-3′), and C1qC (sense: 5′-cgatacaaacagaagcaccag-3′, antisense: 5′-ctggcaaggttgaggttcag-3′) with the following parameters: 94°C for 2 minutes followed by 40 cycles at 94°C for 45 s, 62°C for 60 s, 72°C for 30 s and a final incubation at 72°C for 10 minutes. ..

    Mutagenesis:

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance
    Article Snippet: .. The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer. .. The fraction of DNA bound was quantified by densitometry using AlphaView SA software (Alpha Innotech), and then plotted against enzyme concentration to determine Kd values by interpolation.

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance
    Article Snippet: .. TaqMan qPCR reactions consisted of 10 ng wild-type or mutant Taq , human genomic DNA, 0.8 mM dNTPs, 1× Taq buffer (adjusted to 95 mM KCl for Taq mutants), and 1× β-actin primer/probe (171 bp) from Life Technologies' Assays-On-Demand. qPCR reactions were run on the StepOnePlus (Life Technologies) or CFX96 (BioRad) instrument using cycling parameters indicated in the Figure legends. .. Primer extension assays Extension rate and processivity were measured at 70°C using M13mp18 template DNA (NEB), pre-annealed at a 1.3–1.5:1 molar ratio to a 5′ 33 P-labeled primer with the sequence 5′-GGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGC-3′.

    Purification:

    Article Title: Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
    Article Snippet: .. Linearized DNA was purified and used as template in PCR reactions containing 5 U Taq DNAP, standard Taq buffer with 1.5 mM Mg2+ , 200 µM dNTPs (Invitrogen), CaCl2 to indicated concentrations and 0.5 µM each of the primers CLA55 ( 5′-AGCTTATCGATAAGCGATGCCGGGAGCAGACAAGC-3′ ) and CLA33 ( 5′-AGCTTATCGATGGCACTTTTCGGGGAAATGTGCG-3′ ). .. PCR products were purified using a DNA Clean and Concentrator-5 kit (Zymo Research).

    Real-time Polymerase Chain Reaction:

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance
    Article Snippet: .. TaqMan qPCR reactions consisted of 10 ng wild-type or mutant Taq , human genomic DNA, 0.8 mM dNTPs, 1× Taq buffer (adjusted to 95 mM KCl for Taq mutants), and 1× β-actin primer/probe (171 bp) from Life Technologies' Assays-On-Demand. qPCR reactions were run on the StepOnePlus (Life Technologies) or CFX96 (BioRad) instrument using cycling parameters indicated in the Figure legends. .. Primer extension assays Extension rate and processivity were measured at 70°C using M13mp18 template DNA (NEB), pre-annealed at a 1.3–1.5:1 molar ratio to a 5′ 33 P-labeled primer with the sequence 5′-GGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGC-3′.

    Incubation:

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance
    Article Snippet: .. The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer. .. The fraction of DNA bound was quantified by densitometry using AlphaView SA software (Alpha Innotech), and then plotted against enzyme concentration to determine Kd values by interpolation.

    Article Title: Rod Photoreceptor Ribbon Synapses in DBA/2J Mice Show Progressive Age-Related Structural Changes
    Article Snippet: .. PCR amplification was performed using 1 µl cDNA as a template in 10 µl PCR buffer (20 mM Tris–HCl pH 8.0, 50 mM KCl, 2.5 mM MgCl2 , 0.2 mM dNTPs, 2.5 mM, 1 U Taq-polymerase; Invitrogen) in a programmable thermocycler (GeneAmp PCR System 9700; Applied Biosystems, Foster City, CA) using primer pairs specific for C1qA (sense: 5′-agctgctggcatccggac-3′, antisense: 5′-ggtcccacttggagatcac-3′), C1qB (sense: 5′-cctgaggaccatcaacagc-3′, antisense: 5′-ctcctcttgctctagcttc-3′), and C1qC (sense: 5′-cgatacaaacagaagcaccag-3′, antisense: 5′-ctggcaaggttgaggttcag-3′) with the following parameters: 94°C for 2 minutes followed by 40 cycles at 94°C for 45 s, 62°C for 60 s, 72°C for 30 s and a final incubation at 72°C for 10 minutes. ..

    Polymerase Chain Reaction:

    Article Title: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
    Article Snippet: .. Multiplex conventional PCR (cPCR) Conventional PCR assays were carried out in a final volume of 50 μl, containing: 5 μl DNA (20–25 ng), 5 μl 10× Taq Platinum buffer, 0.2 mM dNTPs, 4.5 mM MgCl2 , 1.25 U Taq Platinum DNA polymerase (Life Technologies, Carlsbad, CA, USA), 200 nM 121/122 primers (T. cruzi kDNA) [ , ] and 100 nM P2B/P6R primers (triatomine 12S rRNA gene). ..

    Article Title: Rod Photoreceptor Ribbon Synapses in DBA/2J Mice Show Progressive Age-Related Structural Changes
    Article Snippet: .. PCR amplification was performed using 1 µl cDNA as a template in 10 µl PCR buffer (20 mM Tris–HCl pH 8.0, 50 mM KCl, 2.5 mM MgCl2 , 0.2 mM dNTPs, 2.5 mM, 1 U Taq-polymerase; Invitrogen) in a programmable thermocycler (GeneAmp PCR System 9700; Applied Biosystems, Foster City, CA) using primer pairs specific for C1qA (sense: 5′-agctgctggcatccggac-3′, antisense: 5′-ggtcccacttggagatcac-3′), C1qB (sense: 5′-cctgaggaccatcaacagc-3′, antisense: 5′-ctcctcttgctctagcttc-3′), and C1qC (sense: 5′-cgatacaaacagaagcaccag-3′, antisense: 5′-ctggcaaggttgaggttcag-3′) with the following parameters: 94°C for 2 minutes followed by 40 cycles at 94°C for 45 s, 62°C for 60 s, 72°C for 30 s and a final incubation at 72°C for 10 minutes. ..

    Article Title: High-Fidelity DNA Polymerase Enhances the Sensitivity of a Peptide Nucleic Acid Clamp PCR Assay for K-ras Mutations
    Article Snippet: .. The Platinum Taq -based PNA clamp PCR assay was identical to the Phusion HS assay, except for the use of 1× Platinum Taq PCR buffer (Invitrogen, Carlsbad, CA), 3 mmol/L MgCl2 , and 0.02 U/μl Platinum Taq DNA polymerase. .. The denaturation temperature was 94°C and the initial denaturation and enzyme activation step lasted 2 minutes, as recommended by the manufacturer.

    Article Title: Mutations in the MDR3 gene cause progressive familial intrahepatic cholestasis
    Article Snippet: .. PCRs were carried out in a Perkin–Elmer GeneAmp PCR system 2400, in 1× Taq polymerase buffer (Life Technologies)/1.5 mM MgCl2 /all four dNTPs (each at 0.5 mM)/each primer at 400 nM/0.5 unit of Taq polymerase. .. The PCR was terminated after an extension step at 72°C for 7 min.

    Article Title: Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing
    Article Snippet: .. Linearized DNA was purified and used as template in PCR reactions containing 5 U Taq DNAP, standard Taq buffer with 1.5 mM Mg2+ , 200 µM dNTPs (Invitrogen), CaCl2 to indicated concentrations and 0.5 µM each of the primers CLA55 ( 5′-AGCTTATCGATAAGCGATGCCGGGAGCAGACAAGC-3′ ) and CLA33 ( 5′-AGCTTATCGATGGCACTTTTCGGGGAAATGTGCG-3′ ). .. PCR products were purified using a DNA Clean and Concentrator-5 kit (Zymo Research).

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  • 99
    Thermo Fisher taq polymerase buffer
    <t>Primase</t> vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and <t>Taq</t> polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.
    Taq Polymerase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq polymerase buffer/product/Thermo Fisher
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    taq polymerase buffer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher advantage2 taq polymerase buffer
    <t>Primase</t> vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and <t>Taq</t> polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.
    Advantage2 Taq Polymerase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage2 taq polymerase buffer/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    advantage2 taq polymerase buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Thermo Fisher taq
    <t>Primase</t> vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and <t>Taq</t> polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.
    Taq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq/product/Thermo Fisher
    Average 90 stars, based on 286 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.

    Journal: Nucleic Acids Research

    Article Title: A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    doi: 10.1093/nar/gkt1385

    Figure Lengend Snippet: Primase vs. primer-dependent DNA polymerase activity of intact PolpTN2, PolPTNΔ 311–923 and Taq polymerase. ( A ) Intact PolpTN2 (40 ng/µl), ( B ) PolpTN2Δ 311–923 (80 ng/µl) or ( C ) Taq DNA polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA, which was or was not hybridized to the complementary oligonucleotide primer M13 forward ( Supplementary Table S1 ). At t = 0, 5, 10 and 15 min, aliquots were withdrawn and quenched into 25 mM EDTA. The amount of ds DNA synthesized was then determined using Sybr® Green I fluorescence as described in Materials and Methods. Circles, continuous line: synthesis with hybridized primer; squares, dashed line: synthesis without primer. Points are the average of two determinations. The standard deviation is indicated by error bars.

    Article Snippet: Non-radioactive primase and DNA polymerase assays Reactions were carried out in Taq polymerase buffer (75 mM Tris-HCl pH 8.8 at 25°C, 20 mM (NH4 )2 SO4 , 0.01% Tween 20) (Thermo) containing 2.5 mM MgCl2 , 0.4 mM of each of the four dNTPs, 2 ng/µl of M13mp18 single-stranded DNA (New England Biolabs) and enzyme components as described in the text.

    Techniques: Activity Assay, Incubation, Synthesized, SYBR Green Assay, Fluorescence, Standard Deviation

    Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.

    Journal: Nucleic Acids Research

    Article Title: A highly divergent archaeo-eukaryotic primase from the Thermococcus nautilus plasmid, pTN2

    doi: 10.1093/nar/gkt1385

    Figure Lengend Snippet: Efficiency of intact PolpTN2 and PolpTN2Δ 311–923 in priming DNA synthesis by T. nautilus PolB and Taq DNA polymerase. T. nautilus PolB (8 ng/µl) or Taq polymerase (0,05 u/µl) were incubated at 70°C in Taq buffer + 0,4 mM dNTP and 2 ng/µl M13mp18 DNA (without annealed primer) with increasing concentrations of either intact PolpTN2 ( A ) or PolpTN2 Δ 311–923 ( B ). DNA synthesis by intact PolpTN2 or PolpTN2 Δ 311–923 alone was included as a control. At t = 0 and 6 min, aliquots were withdrawn, quenched in 25 mM EDTA and assayed for ds DNA using Sybr® Green I fluorescence. Circles, continuous line: PolpTN2 or PolpTN2 Δ 311–923 alone; squares, dashed line: PolpTN2 or PolpTN2 Δ 311–923 plus Taq DNA polymerase; triangles, dotted line: PolpTN2 or PolpTN2 Δ 311–923 plus T. nautilus PolB DNA polymerase. Points are the average of three determinations. The standard deviation is indicated by error bars.

    Article Snippet: Non-radioactive primase and DNA polymerase assays Reactions were carried out in Taq polymerase buffer (75 mM Tris-HCl pH 8.8 at 25°C, 20 mM (NH4 )2 SO4 , 0.01% Tween 20) (Thermo) containing 2.5 mM MgCl2 , 0.4 mM of each of the four dNTPs, 2 ng/µl of M13mp18 single-stranded DNA (New England Biolabs) and enzyme components as described in the text.

    Techniques: DNA Synthesis, Incubation, SYBR Green Assay, Fluorescence, Standard Deviation