taq pcr core kit  (Qiagen)


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    Structured Review

    Qiagen taq pcr core kit
    Taq Pcr Core Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq pcr core kit/product/Qiagen
    Average 99 stars, based on 197 article reviews
    Price from $9.99 to $1999.99
    taq pcr core kit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    RNA Extraction:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: Paragraph title: RNA Extraction and cDNA Synthesis. ... Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    Amplification:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: Paragraph title: PCR Amplification ... PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization.

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    Agarose Gel Electrophoresis:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. PCR products were resolved in 2% agarose gel and visualized under UV with Redsafe (FroggaBio, North York, ON, Canada) staining.

    Binding Assay:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. Real-time PCR analysis for mouse ApoD, Opg, Rankl and Sod1 was performed using the iCycler IQ detection system (Bio-Rad, Hercules, CA, USA) and SYBR Green I (Bio-Rad) as a doublestrand DNA-specific binding dye, using β-microglobulin (B2m) as a reference gene (F: 5′-TACTCAGCCACCCACCGGCCG-3′, R: 5′-GCTCGGCCATACTGGCATGCT-3′) and the following sets of specific primers: Opg (F: 5′-GTGTGGAATAGATGTCACCCTGT-3′, R: 5′-CTTGTGAGCTGTGTCTCCGT-3′), Rankl (F: 5′-CCCATCG GGTTCCCATAAAGT-3′, R: 5′-AGCAAATGTTGGCGTACAGG-3′) and Sod1 (F: 5′-CACTTCGAGCAGAAGGCAAG-3′, R: 5′-CCCCATA CTGATGGACGTGG-3′).

    Fluorescence:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. Each sample was run in triplicate, and fluorescence data were collected at the end of each cycle.

    Synthesized:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    Isolation:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    SYBR Green Assay:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. Real-time PCR analysis for mouse ApoD, Opg, Rankl and Sod1 was performed using the iCycler IQ detection system (Bio-Rad, Hercules, CA, USA) and SYBR Green I (Bio-Rad) as a doublestrand DNA-specific binding dye, using β-microglobulin (B2m) as a reference gene (F: 5′-TACTCAGCCACCCACCGGCCG-3′, R: 5′-GCTCGGCCATACTGGCATGCT-3′) and the following sets of specific primers: Opg (F: 5′-GTGTGGAATAGATGTCACCCTGT-3′, R: 5′-CTTGTGAGCTGTGTCTCCGT-3′), Rankl (F: 5′-CCCATCG GGTTCCCATAAAGT-3′, R: 5′-AGCAAATGTTGGCGTACAGG-3′) and Sod1 (F: 5′-CACTTCGAGCAGAAGGCAAG-3′, R: 5′-CCCCATA CTGATGGACGTGG-3′).

    Polymerase Chain Reaction:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: .. PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. PCR products were resolved in 2% agarose gel and visualized under UV with Redsafe (FroggaBio, North York, ON, Canada) staining.

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    Staining:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. PCR products were resolved in 2% agarose gel and visualized under UV with Redsafe (FroggaBio, North York, ON, Canada) staining.

    Real-time Polymerase Chain Reaction:

    Article Title: Apolipoprotein D deficiency is associated to high bone turnover, low bone mass and impaired osteoblastic function in aged female mice.
    Article Snippet: PCR amplifications for semi-quantitative analysis were conducted with Taq PCR core kit (Qiagen) using specific primer sets for murine ApoD (F: 5′-CCACCGGCACCCTACTGGATC-3′, R: 5′-CGGGCAGTTCGCTTGATCTGT-3′) and human ApoD (F: 5′-TTA ACCTCACAGAGCCTGCC-3′, R: 5′-GAGTCCACTGTTTCTGGAG GG-3′); Gapdh (F: 5′-AGAACATCATCCCTGCATCC-3′, R: 5′-AGTT GCTGTTGACGTCGC-3′) was used as a reference gene for normalization. .. Real-time PCR analysis for mouse ApoD, Opg, Rankl and Sod1 was performed using the iCycler IQ detection system (Bio-Rad, Hercules, CA, USA) and SYBR Green I (Bio-Rad) as a doublestrand DNA-specific binding dye, using β-microglobulin (B2m) as a reference gene (F: 5′-TACTCAGCCACCCACCGGCCG-3′, R: 5′-GCTCGGCCATACTGGCATGCT-3′) and the following sets of specific primers: Opg (F: 5′-GTGTGGAATAGATGTCACCCTGT-3′, R: 5′-CTTGTGAGCTGTGTCTCCGT-3′), Rankl (F: 5′-CCCATCG GGTTCCCATAAAGT-3′, R: 5′-AGCAAATGTTGGCGTACAGG-3′) and Sod1 (F: 5′-CACTTCGAGCAGAAGGCAAG-3′, R: 5′-CCCCATA CTGATGGACGTGG-3′).

    Rapid Amplification of cDNA Ends:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

    Nucleic Acid Electrophoresis:

    Article Title: Salt-water acclimation of the estuarine crocodile Crocodylus porosus involves enhanced ion transport properties of the urodaeum and rectum.
    Article Snippet: Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments. .. Estuarine crocodiles, < i > Crocodylus porosus < /i > , inhabit freshwater, estuarine and marine environments.

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  • 99
    Qiagen taq pcr core kit
    Representative CAG repeat distributions, and Msh3 variations in B6 and CBy mice. A) The autoradiographs show representative <t>SP-PCR</t> analyses of DNA, extracted from heart, liver, striatum and tail. At weaning the B6.Cg-R6/1 (B6) and CBy.Cg-R6/1 (CBy) congenic mice contained in tail DNA (CAG)98 and (CAG)94, respectively. For comparison the profiles of the Msh2 −/− mouse is shown. About 5–10 DNA amplifiable molecules were amplified in each reaction with primers MS-1F and MS-1R. Animals were 20-weeks old. B) Congenic CBy.Cg-R6/1 mice were crossed to B6 and the resulting F1 progeny were crossed to produce F2 mice with all possible genotypes at the Msh3 locus. Repeat instability was assayed by amplifying 10 ng genomic DNA using fluorescently labelled primers and resolving the fragments by capillary gel electrophoresis ( Figure 1B ). Using this high-resolution approach repeat length distributions present with the typical ‘hedgehog’ pattern ( e.g. [10] , [13] , [15] , [16] . This pattern reflects both somatic mosaicism within the sample and PCR artefacts generated by <t>Taq</t> polymerase slippage [62] , [63] . The PCR artefacts are predominantly repeat contractions, hence these are not considered here. The pattern of CAG repeat instability depended on genotype at the MSH3 locus. B6 homozygosity resulted in the greatest instability, CBy homozygosity resulted in lack of expansion, while heterozygosity resulted in an intermediate instability, indicative of a gene dosage effect of the Msh3 locus. Numbers indicate the CAG repeat size corresponding to major peaks. In addition, on the B6 tracing, a second number indicates the highest CAG repeat number detected. C) Msh3 polymorphisms in Msh3 gene from C57BL/6 (B6) and BALB/cBy (CBy) mice. Promoters were identical. SNPs were identified or confirmed to those in dbSNP by sequencing the Msh3 gene.
    Taq Pcr Core Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq pcr core kit/product/Qiagen
    Average 99 stars, based on 222 article reviews
    Price from $9.99 to $1999.99
    taq pcr core kit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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    Representative CAG repeat distributions, and Msh3 variations in B6 and CBy mice. A) The autoradiographs show representative SP-PCR analyses of DNA, extracted from heart, liver, striatum and tail. At weaning the B6.Cg-R6/1 (B6) and CBy.Cg-R6/1 (CBy) congenic mice contained in tail DNA (CAG)98 and (CAG)94, respectively. For comparison the profiles of the Msh2 −/− mouse is shown. About 5–10 DNA amplifiable molecules were amplified in each reaction with primers MS-1F and MS-1R. Animals were 20-weeks old. B) Congenic CBy.Cg-R6/1 mice were crossed to B6 and the resulting F1 progeny were crossed to produce F2 mice with all possible genotypes at the Msh3 locus. Repeat instability was assayed by amplifying 10 ng genomic DNA using fluorescently labelled primers and resolving the fragments by capillary gel electrophoresis ( Figure 1B ). Using this high-resolution approach repeat length distributions present with the typical ‘hedgehog’ pattern ( e.g. [10] , [13] , [15] , [16] . This pattern reflects both somatic mosaicism within the sample and PCR artefacts generated by Taq polymerase slippage [62] , [63] . The PCR artefacts are predominantly repeat contractions, hence these are not considered here. The pattern of CAG repeat instability depended on genotype at the MSH3 locus. B6 homozygosity resulted in the greatest instability, CBy homozygosity resulted in lack of expansion, while heterozygosity resulted in an intermediate instability, indicative of a gene dosage effect of the Msh3 locus. Numbers indicate the CAG repeat size corresponding to major peaks. In addition, on the B6 tracing, a second number indicates the highest CAG repeat number detected. C) Msh3 polymorphisms in Msh3 gene from C57BL/6 (B6) and BALB/cBy (CBy) mice. Promoters were identical. SNPs were identified or confirmed to those in dbSNP by sequencing the Msh3 gene.

    Journal: PLoS Genetics

    Article Title: MSH3 Polymorphisms and Protein Levels Affect CAG Repeat Instability in Huntington's Disease Mice

    doi: 10.1371/journal.pgen.1003280

    Figure Lengend Snippet: Representative CAG repeat distributions, and Msh3 variations in B6 and CBy mice. A) The autoradiographs show representative SP-PCR analyses of DNA, extracted from heart, liver, striatum and tail. At weaning the B6.Cg-R6/1 (B6) and CBy.Cg-R6/1 (CBy) congenic mice contained in tail DNA (CAG)98 and (CAG)94, respectively. For comparison the profiles of the Msh2 −/− mouse is shown. About 5–10 DNA amplifiable molecules were amplified in each reaction with primers MS-1F and MS-1R. Animals were 20-weeks old. B) Congenic CBy.Cg-R6/1 mice were crossed to B6 and the resulting F1 progeny were crossed to produce F2 mice with all possible genotypes at the Msh3 locus. Repeat instability was assayed by amplifying 10 ng genomic DNA using fluorescently labelled primers and resolving the fragments by capillary gel electrophoresis ( Figure 1B ). Using this high-resolution approach repeat length distributions present with the typical ‘hedgehog’ pattern ( e.g. [10] , [13] , [15] , [16] . This pattern reflects both somatic mosaicism within the sample and PCR artefacts generated by Taq polymerase slippage [62] , [63] . The PCR artefacts are predominantly repeat contractions, hence these are not considered here. The pattern of CAG repeat instability depended on genotype at the MSH3 locus. B6 homozygosity resulted in the greatest instability, CBy homozygosity resulted in lack of expansion, while heterozygosity resulted in an intermediate instability, indicative of a gene dosage effect of the Msh3 locus. Numbers indicate the CAG repeat size corresponding to major peaks. In addition, on the B6 tracing, a second number indicates the highest CAG repeat number detected. C) Msh3 polymorphisms in Msh3 gene from C57BL/6 (B6) and BALB/cBy (CBy) mice. Promoters were identical. SNPs were identified or confirmed to those in dbSNP by sequencing the Msh3 gene.

    Article Snippet: Qiagen Taq polymerase (cat #201225) was used as recommended by the manufacturer.

    Techniques: Mouse Assay, Polymerase Chain Reaction, Amplification, Mass Spectrometry, Nucleic Acid Electrophoresis, Generated, Sequencing

    Typical light microscopic aspect of a basaloid squamous cell carcinoma (BSCC) composed of cells with hyperchromatic nuclei and scant cyctoplasm ( a ); H E; original magnification, ×400. Strong expression of Bcl-2 protein (brown cytoplasmatic reaction product) in an esophageal BSCC. Note the absence of Bcl-2 expression in the neighboring nonmalignant esophageal squamous epithelium ( b ); original magnification, ×400. Ethidium bromide-stained agarose gel of a differential PCR for the c-myc gene and the APRT gene in carcinoma tissues (Ca) and corresponding normal tissues (Normal) from 4 cases of esophageal BSCC. Note amplification of the c-myc gene in tumors 1 and 2, no c-myc amplification in tumors 3 and 4 ( c ).

    Journal: The American Journal of Pathology

    Article Title: Expression of Bcl-2 and Amplification of c-myc Are Frequent in Basaloid Squamous Cell Carcinomas of the Esophagus

    doi:

    Figure Lengend Snippet: Typical light microscopic aspect of a basaloid squamous cell carcinoma (BSCC) composed of cells with hyperchromatic nuclei and scant cyctoplasm ( a ); H E; original magnification, ×400. Strong expression of Bcl-2 protein (brown cytoplasmatic reaction product) in an esophageal BSCC. Note the absence of Bcl-2 expression in the neighboring nonmalignant esophageal squamous epithelium ( b ); original magnification, ×400. Ethidium bromide-stained agarose gel of a differential PCR for the c-myc gene and the APRT gene in carcinoma tissues (Ca) and corresponding normal tissues (Normal) from 4 cases of esophageal BSCC. Note amplification of the c-myc gene in tumors 1 and 2, no c-myc amplification in tumors 3 and 4 ( c ).

    Article Snippet: Differential PCR was performed in a final volume of 50 μl with 2 μl of DNA template, PCR buffer containing 1.5 mmol/L MgCl2 , 0.2 mmol/L of each dNTP, 30 pmol primer for the c-myc gene, 60 pmol primer for the APRT gene, and 2 U Taq DNA-polymerase (PCR Core Kit, Qiagen, Hilden, Germany).

    Techniques: Expressing, Staining, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification

    Real-time PCR detection of deer mouse transcription factors . Total RNA was extracted from 42 hour cultures of T cells with or without KLH activation and used for cDNA synthesis. Real-time PCR was performed using primers described in Table 1 to detect the expression of transcription factors associated with Th1, Th2 or Treg T cells. The mean CT values and standard deviations from triplicate samples obtained from the instrument were subtracted from the number of cycles performed (50) to determine adjusted CT means for unstimulated (-K) and stimulated (KLH) T cell cultures (A). The adjusted CT mean values were then used to calculated the cycle difference (CD) by subtracting the -K value from the KLH value. The relative template abundance (RTA) was then determined by calculating the log 2 value of the CD (2 CD ) (B).

    Journal: BMC Immunology

    Article Title: Profiling helper T cell subset gene expression in deer mice

    doi: 10.1186/1471-2172-7-18

    Figure Lengend Snippet: Real-time PCR detection of deer mouse transcription factors . Total RNA was extracted from 42 hour cultures of T cells with or without KLH activation and used for cDNA synthesis. Real-time PCR was performed using primers described in Table 1 to detect the expression of transcription factors associated with Th1, Th2 or Treg T cells. The mean CT values and standard deviations from triplicate samples obtained from the instrument were subtracted from the number of cycles performed (50) to determine adjusted CT means for unstimulated (-K) and stimulated (KLH) T cell cultures (A). The adjusted CT mean values were then used to calculated the cycle difference (CD) by subtracting the -K value from the KLH value. The relative template abundance (RTA) was then determined by calculating the log 2 value of the CD (2 CD ) (B).

    Article Snippet: Cloning of transcription factor cDNAs Primers from highly conserved regions of orthologous T-bet, GATA-3, Fox-p3, STAT4 and STAT6 cDNA sequences were used to amplify deer mouse genes using the PCR Core kit with its Q-solution (Qiagen, Valencia, CA).

    Techniques: Real-time Polymerase Chain Reaction, Activation Assay, Expressing

    Real-time PCR detection of deer mouse cytokine gene expression . Template cDNA was from RNA samples described in Figure 2. Real-time PCR was performed using primers described in Table 1. Adjusted mean CT values for both antigen-stimulated and unstimulated cells (A) and the calculated RTA values for each cytokine cDNA (B).

    Journal: BMC Immunology

    Article Title: Profiling helper T cell subset gene expression in deer mice

    doi: 10.1186/1471-2172-7-18

    Figure Lengend Snippet: Real-time PCR detection of deer mouse cytokine gene expression . Template cDNA was from RNA samples described in Figure 2. Real-time PCR was performed using primers described in Table 1. Adjusted mean CT values for both antigen-stimulated and unstimulated cells (A) and the calculated RTA values for each cytokine cDNA (B).

    Article Snippet: Cloning of transcription factor cDNAs Primers from highly conserved regions of orthologous T-bet, GATA-3, Fox-p3, STAT4 and STAT6 cDNA sequences were used to amplify deer mouse genes using the PCR Core kit with its Q-solution (Qiagen, Valencia, CA).

    Techniques: Real-time Polymerase Chain Reaction, Expressing