taq ligase buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs taq ligase buffer
    Taq Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq ligase buffer/product/New England Biolabs
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    taq ligase buffer - by Bioz Stars, 2020-04
    91/100 stars

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    Mutagenesis:

    Article Title: Single Quantum Dot Analysis Enables Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction
    Article Snippet: .. Gap-LCR was conducted in 25 μl of reaction volume containing ThermoPol Buffer (1X); Taq Ligase Buffer (0.25X); NAD β (1mM); dGTP (10 μM); dATP (10 μM); MgCl2 (3 mM); Taq DNA Polymerase (1.25 units, New England Biolabs); Taq DNA Ligase (10 units, New England Biolabs); various amounts of mutant and/or wild type templates (synthetic or genetic DNA extracted from cell lines); common primers (60 nM, Cpr1 – 3) as well as discrimination primers (Apr and/or Gpr). .. The mixture was first incubated at 94 °C for 2 minutes, and then thermal cycling was conducted for 60 cycles at 94 °C for 30 seconds (denaturation step) and 65.1 °C for 60 seconds (ligation step) unless otherwise noted ( ).

    Incubation:

    Article Title: Single Quantum Dot Analysis Enables Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction
    Article Snippet: Gap-LCR was conducted in 25 μl of reaction volume containing ThermoPol Buffer (1X); Taq Ligase Buffer (0.25X); NAD β (1mM); dGTP (10 μM); dATP (10 μM); MgCl2 (3 mM); Taq DNA Polymerase (1.25 units, New England Biolabs); Taq DNA Ligase (10 units, New England Biolabs); various amounts of mutant and/or wild type templates (synthetic or genetic DNA extracted from cell lines); common primers (60 nM, Cpr1 – 3) as well as discrimination primers (Apr and/or Gpr). .. The mixture was first incubated at 94 °C for 2 minutes, and then thermal cycling was conducted for 60 cycles at 94 °C for 30 seconds (denaturation step) and 65.1 °C for 60 seconds (ligation step) unless otherwise noted ( ).

    Ligation:

    Article Title: Single Quantum Dot Analysis Enables Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction
    Article Snippet: Gap-LCR was conducted in 25 μl of reaction volume containing ThermoPol Buffer (1X); Taq Ligase Buffer (0.25X); NAD β (1mM); dGTP (10 μM); dATP (10 μM); MgCl2 (3 mM); Taq DNA Polymerase (1.25 units, New England Biolabs); Taq DNA Ligase (10 units, New England Biolabs); various amounts of mutant and/or wild type templates (synthetic or genetic DNA extracted from cell lines); common primers (60 nM, Cpr1 – 3) as well as discrimination primers (Apr and/or Gpr). .. The mixture was first incubated at 94 °C for 2 minutes, and then thermal cycling was conducted for 60 cycles at 94 °C for 30 seconds (denaturation step) and 65.1 °C for 60 seconds (ligation step) unless otherwise noted ( ).

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    New England Biolabs taq dna ligase buffer
    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, <t>Taq</t> <t>DNA</t> polymerase; and Vn, Vent DNA polymerase).
    Taq Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase buffer/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    90
    New England Biolabs dna ligase buffer
    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, <t>DNA</t> replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to <t>T4.</t> The genetic map was created using the Geneious software.
    Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligase buffer/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dna ligase buffer - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    Image Search Results


    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Journal: Nucleic Acids Research

    Article Title: Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

    doi: 10.1093/nar/gni058

    Figure Lengend Snippet: Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Article Snippet: Synthesis of double-stranded DNA was performed by thermal cycling of 10 μl phosphorylated sense oligonucleotides, 10 μl phosphorylated antisense oligonucleotides, 3 μl 10× Taq DNA ligase buffer (NEB) in a total volume of 30 μl.

    Techniques: Functional Assay, Clone Assay, Activity Assay, Amplification, Incubation

    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only

    Journal: Nucleic Acids Research

    Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

    doi: 10.1093/nar/gnh045

    Figure Lengend Snippet: Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only

    Article Snippet: Ligation reactions for Arabidopsis polymorphisms were performed in a 25 µl volume containing 625 ng of Arabidopsis DNA, 1× Taq DNA ligase buffer [20 mM Tris–HCl, 25 mM KAc, 10 mM MgAc2 , 10 mM dithiothreitol (DTT), 1 mM NAD, 0.1% Triton X-100; pH 7.6 at 25°C; New England Biolabs Inc., Beverly, MA], 0.2 U/µl Taq DNA ligase (NEB) and 0.05 fmol/µl of each of 200 ligation probes.

    Techniques: Ligation

    The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Journal: Viruses

    Article Title: Genomic Characterization of Sixteen Yersinia enterocolitica-Infecting Podoviruses of Pig Origin

    doi: 10.3390/v10040174

    Figure Lengend Snippet: The genomic map of fPS-7. The predicted genes are arranged in the direction of transcription shown by different colored arrows. Genes involved in nucleotide metabolism, DNA replication, recombination or repair are shown in green. Genes involved in morphogenesis and virion structures are depicted in brown. Genes involved in DNA packaging and lysis, are shown in blue and red, respectively. Genes coding for hypothetical proteins or conserved phage proteins of unknown function are shown in light grey. Homing endonucleases are shown in yellow. Direct terminal repeats (DTRs) are shown in black. On top of the genome, the host RNA polymerase (RNAP)-dependent promoters are shown with red double-arrows labelled with −35 and −10, and the phage RNAP-dependent promoters with black arrows labelled from P1 to P12. Terminators are shown along the genome as purple triangles and labelled from T1 to T4. The genetic map was created using the Geneious software.

    Article Snippet: The kinase was then inactivated at 75 °C for 15 min. Ligation of the phosphorylated DNA to a 500 bp fragment of the fliC gene of Y. enterocolitica O:3 was performed in a 30 µL reaction mixture containing ~400 ng of the phosphorylated DNA, ~40 ng of fliC gene fragment, 2 µL of 50% ( w / v ) PEG 4000 (Thermo Scientific), 3 µL of 10 × T4 µL DNA Ligase buffer and 1 µL T4 DNA ligase (New England BioLabs).

    Techniques: Lysis, Software