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Promega taq i
Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and <t>Mse</t> I restriction endonuclease sites.
Taq I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States"

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

Journal: Ecology and Evolution

doi: 10.1002/ece3.639

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

2) Product Images from "Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)"

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)

Journal: Brazilian Journal of Microbiology

doi: 10.1590/S1517-838246220120018

Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.
Figure Legend Snippet: Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

Techniques Used: Construct, Generated

3) Product Images from "Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California"

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.39.4.1221-1226.2001

PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .
Figure Legend Snippet: PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

Techniques Used: Polymerase Chain Reaction

4) Product Images from "Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting"

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting

Journal: BMC Microbiology

doi: 10.1186/1471-2180-14-52

Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).
Figure Legend Snippet: Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).

Techniques Used: Sequencing, Polymerase Chain Reaction

5) Product Images from "Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer"

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer

Journal: Genetics and Molecular Biology

doi: 10.1590/S1415-47572012005000074

Genotype determination by using PCR-RFLP of the NAT2 gene fragment. Following PCR amplification separate digestions of each PCR product were carried out with the restriction enzymes Kpn I, Taq I, Bam H I and Alu I/ Msp I to detect the substitutions C481T, G590A, G857A and G191A, respectively. The different sizes of the digested products for each restriction enzyme which allow the individual’s genotype to be determined are shown diagrammatically.
Figure Legend Snippet: Genotype determination by using PCR-RFLP of the NAT2 gene fragment. Following PCR amplification separate digestions of each PCR product were carried out with the restriction enzymes Kpn I, Taq I, Bam H I and Alu I/ Msp I to detect the substitutions C481T, G590A, G857A and G191A, respectively. The different sizes of the digested products for each restriction enzyme which allow the individual’s genotype to be determined are shown diagrammatically.

Techniques Used: Polymerase Chain Reaction, Amplification

6) Product Images from "Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States"

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

Journal: Ecology and Evolution

doi: 10.1002/ece3.639

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

7) Product Images from "The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates"

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

Journal: Journal of Veterinary Science

doi: 10.4142/jvs.2006.7.3.277

PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
Figure Legend Snippet: PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis

Related Articles

Amplification:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: The PCR amplification was performed with 10 μl of sample in a 100-μl reaction mixture containing 50 mM KCl, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2 , 0.001% gelatin, 0.1% Brij 35, 200 μM (each) deoxynucleotide triphosphate (dATP, dCTP, dGTP, and dTTP), 0.5 μM (each) primer Bh CS781.p and Bh CS1137.n, and 0.2 U of thermostable Ampli- Taq DNA polymerase (Perkin-Elmer Cetus, Norwalk, Conn.). .. A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ).

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: Briefly PCR amplification using primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TC CTCCGCTTATTGATATGC-3′) was carried out to amplify a polymorphic ITS fragment. .. The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions.

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: The amplified ITS fragment was analyzed by 2.0% (w/v) agarose gel electrophoresis at 80 V in 0.5× TBE (45 mM Tris-borate, 1 mM EDTA, pH 8.0) buffer to check its intactness and absence of non-specific amplification. .. The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions.

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: .. Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ]. .. Restricted fragments were examined by electrophoresis on 2 % agarose gels and the profiles were compared to those of the 7 species that were detected to date in dog blood samples in Europe B. caballi , B. canis , B. gibsoni , B. rossi , B. vogeli , T. equi, and T. annae obtained from infected animals or parasite cultures provided by different laboratories.

Article Title: Serotonergic Regulation of Membrane Potential in Developing Rat Prefrontal Cortex: Coordinated Expression of 5-Hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 Receptors
Article Snippet: The amplification was performed in 10 m m Tris/HCl, 50 m m KCl, 1.5–2.5 m m MgCl, 200 m m dNTP mix, and 200 nmol of each primer, pH 8.3, with 5 U of Taq DNA polymerase (HotStarTaq Master Mix Kit; Qiagen, Chatsworth, CA). .. Restriction digest used Xho I for the 5-HT2A receptor and Taq I for the 5-HT7 receptor (Promega, Madison, WI).

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA). .. The digested fragments were separated on Agarose gels stained with ethidium bromide and visualized under UV light.

Positive Control:

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: The positive control of B. vinsonii subsp. berkhoffii was systematically prepared last, to prevent possible cross contamination with the tested samples. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

Synthesized:

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: PCR-RFLP Using Gene Runner software (Hastings Software, USA), a 23S rRNA-specific primer set was designed and synthesized (Bionics, Korea). .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions.

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: NAT2 -specific primers were synthesized by Alpha DNA Inc. (Montreal, Canada). .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA).

Terminal Restriction Fragment Length Polymorphism:

Article Title: A Microbial Link between Elevated CO2 and Methane Emissions that is Plant Species-Specific
Article Snippet: Paragraph title: T-RFLP analysis ... An aliquot (16.8 μL) of the PCR products were digested with 5 U Taq I (Promega, Madison, WI), 1× buffer, and 1 μg bovine serum albumin for 2 h at 65 °C.

Article Title: Effect of Temperature on Structure and Function of the Methanogenic Archaeal Community in an Anoxic Rice Field Soil
Article Snippet: Paragraph title: T-RFLP analysis. ... Aliquots of the purified SSU rDNA were digested by Taq I (Promega, Mannheim, Germany).

Electrophoresis:

Article Title: A Microbial Link between Elevated CO2 and Methane Emissions that is Plant Species-Specific
Article Snippet: An aliquot (16.8 μL) of the PCR products were digested with 5 U Taq I (Promega, Madison, WI), 1× buffer, and 1 μg bovine serum albumin for 2 h at 65 °C. .. The samples were sent to the University of Wisconsin-Madison Sequencing Facility for analysis using denaturing capillary electrophoresis on an ABI 3700 genetic analyzer (Applied Biosystems).

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ). .. The digests were analyzed by electrophoresis on a 2% agarose gel in 1× Tris-borate-EDTA buffer.

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μl reaction volume on 2.0% (w/v) agarose gel.

Article Title: Effect of Temperature on Structure and Function of the Methanogenic Archaeal Community in an Anoxic Rice Field Soil
Article Snippet: Aliquots of the purified SSU rDNA were digested by Taq I (Promega, Mannheim, Germany). .. Electrophoresis was performed for 6 h through a 6% (wt/vol) polyacrylamide gel (length, 12 cm) containing 8.3 M urea and 1× Tris-borate-EDTA buffer.

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μL reaction volume on 2.0% (w/v) agarose gel in parallel with PCR 100 bp Low DNA ladder (Sigma-Aldrich) as molecular size standard.

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: Amplified DNA fragments were separated by electrophoresis through 1.5 % agarose gels stained with ethidium bromide and visualized under ultraviolet light. .. Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ].

Nested PCR:

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: To discriminate between species within the Babesia /Theileria positive samples detected by nested PCR, a Restriction Fragment Length Polymorphism method (RFLP) was tested on PCR products from the second round of amplification. .. Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ].

Incubation:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: After incubation for 48 hr at 28°, yeast colonies were subjected to PCR to amplify a polymorphic fragment of the 18S rRNA gene using oligos yeast-F1 and yeast-R1 , or a region comprising the 5.8 rRNA gene and the two sideward regions, ITS1 and ITS2, using primers ITS1F and ITS4 ( ). .. The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions.

Article Title: Effect of Temperature on Structure and Function of the Methanogenic Archaeal Community in an Anoxic Rice Field Soil
Article Snippet: Aliquots of the purified SSU rDNA were digested by Taq I (Promega, Mannheim, Germany). .. Each reaction tube contained 8 μl of the SSU rDNA amplicons, 1 μl of the appropriate incubation buffer supplied by the manufacturer (Promega), and 1 μl of restriction enzyme (10 U), made up to a total volume of 10 μl with deionized H2 O. Incubations were carried out in 0.5-ml reaction tubes for 3 h at 65°C.

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: PCR was initiated after an incubation step at 94℃ for 3 min, followed by 30 cycles of 94℃ for 30 s, 55℃ for 30 s, and 72℃ for 30 s, with a final extension step at 72℃ for 5 min. PCR products that were 517 bp were excised and purified from agarose gels using a Geneclean II Kit (Qbiogene, USA). .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions.

Article Title: Characterization of the mitochondrial genome of Megalobrama terminalis in the Heilong River and a clearer phylogeny of the genus Megalobrama
Article Snippet: .. The PCR components were the same as those mentioned above, and the conditions of the reaction were as follows: 94 °C for 5 min, followed by 30 cycles at 94 °C 30 sec, 56 °C for 30 sec, and 72 °C for 30 sec, and an extension at 72 °C for 5 min. After PCR, 2 µL of RE × 10 buffer, 0.2 µL acetylated BSA and 5 U Taq I (Promega Co., USA) was added to each sample (~1 µg), and the reaction was incubated at 65 °C for 2 h. Then, all digested samples of 7 populations (30 per population) were separated on 4% agarose gels with 0.5 µg/mL ethidium bromide. ..

Genomic Sequencing:

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Article Snippet: The genomic sequences used were as follows: AE016823.1, L.interrogans serovar Copenhageni str. .. PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures.

Infection:

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ]. .. Restricted fragments were examined by electrophoresis on 2 % agarose gels and the profiles were compared to those of the 7 species that were detected to date in dog blood samples in Europe B. caballi , B. canis , B. gibsoni , B. rossi , B. vogeli , T. equi, and T. annae obtained from infected animals or parasite cultures provided by different laboratories.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Serotonergic Regulation of Membrane Potential in Developing Rat Prefrontal Cortex: Coordinated Expression of 5-Hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 Receptors
Article Snippet: Restriction digest used Xho I for the 5-HT2A receptor and Taq I for the 5-HT7 receptor (Promega, Madison, WI). .. Parallel control experiments in which the cellular template for the RT-PCR reaction was replaced by water were invariably negative.

DNA Sequencing:

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions. .. DNA sequencing reactions were performed on an automated DNA sequencer (ABI PRISM 3100 Genetic Analyzer; Applied Biosystems, USA).

Polymerase Chain Reaction:

Article Title: A Microbial Link between Elevated CO2 and Methane Emissions that is Plant Species-Specific
Article Snippet: .. An aliquot (16.8 μL) of the PCR products were digested with 5 U Taq I (Promega, Madison, WI), 1× buffer, and 1 μg bovine serum albumin for 2 h at 65 °C. .. A 625 bp ROX-labeled internal size standard (CHIMERx, Madison, WI) was added to the samples containing 1 μL of the digestion and 10 μL of formamide.

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: Twelve microliters of each PCR-amplified gltA product was used for gltA RFLP analysis. .. A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ).

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: .. The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μl reaction volume on 2.0% (w/v) agarose gel.

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Article Snippet: .. PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures. .. To calculate the relative molecular masses of the digested fragments, a 100-bp DNA Ladder was used (Promega Co.).

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: .. The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μL reaction volume on 2.0% (w/v) agarose gel in parallel with PCR 100 bp Low DNA ladder (Sigma-Aldrich) as molecular size standard.

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: To discriminate between species within the Babesia /Theileria positive samples detected by nested PCR, a Restriction Fragment Length Polymorphism method (RFLP) was tested on PCR products from the second round of amplification. .. Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ].

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions. ..

Article Title: Serotonergic Regulation of Membrane Potential in Developing Rat Prefrontal Cortex: Coordinated Expression of 5-Hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 Receptors
Article Snippet: PCR primers were developed using PCGene software (Intelligenetics, Mountain View, CA). .. Restriction digest used Xho I for the 5-HT2A receptor and Taq I for the 5-HT7 receptor (Promega, Madison, WI).

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA). .. The digested fragments were separated on Agarose gels stained with ethidium bromide and visualized under UV light.

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene. .. Banding patterns were compared to the profiles of B. henselae Houston-1 (ATCC 49882), B. clarridgeiae (ATCC 700095), B. quintana (ATCC VR-358), B. bacilliformis (ATCC 35686), B. elizabethae (ATCC 49927), Bartonella strain cattle-1 (University of California, Davis), B. vinsonii subsp. vinsonii (ATCC VR-152), and B. vinsonii subsp. berkhoffii (ATCC 51672).

Article Title: Characterization of the mitochondrial genome of Megalobrama terminalis in the Heilong River and a clearer phylogeny of the genus Megalobrama
Article Snippet: .. The PCR components were the same as those mentioned above, and the conditions of the reaction were as follows: 94 °C for 5 min, followed by 30 cycles at 94 °C 30 sec, 56 °C for 30 sec, and 72 °C for 30 sec, and an extension at 72 °C for 5 min. After PCR, 2 µL of RE × 10 buffer, 0.2 µL acetylated BSA and 5 U Taq I (Promega Co., USA) was added to each sample (~1 µg), and the reaction was incubated at 65 °C for 2 h. Then, all digested samples of 7 populations (30 per population) were separated on 4% agarose gels with 0.5 µg/mL ethidium bromide. ..

DNA Extraction:

Article Title: A Microbial Link between Elevated CO2 and Methane Emissions that is Plant Species-Specific
Article Snippet: Total community DNA was extracted from soil using the UltraSoil DNA extraction kit (MoBio Laboratories, Solana Beach, CA). .. An aliquot (16.8 μL) of the PCR products were digested with 5 U Taq I (Promega, Madison, WI), 1× buffer, and 1 μg bovine serum albumin for 2 h at 65 °C.

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: Disposable sterile vials and filter tips were used for DNA extraction and PCR reagent preparation. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

Radioactivity:

Article Title: RNase P: role of distinct protein cofactors in tRNA substrate recognition and RNA-based catalysis
Article Snippet: Preparation of RNA and determination of 5′ end group of tRNA A plasmid that harbors the Schizosaccharomyces pombe precursor tRNASer (CUA), pSupS1 , which lacks 3′ CCA sequence, was linearized by Taq I and transcription was done with SP6 RNA polymerase (Promega) in the presence of 80 µCi of [α-32 P]UTP. .. Radioactivity (in counts per minute) was determined by Bioscan QC 2000 beta-counter.

Fluorescence:

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ). .. Gels were stained with ethidium bromide and visualized by UV fluorescence ( ).

Isolation:

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: Paragraph title: Yeast isolation and characterization ... The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions.

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: The PCR chamber was used only for the preparation of PCR reagents and not for the isolation or subculture of Bartonella spp. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

Size-exclusion Chromatography:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: Characterization of the mitochondrial genome of Megalobrama terminalis in the Heilong River and a clearer phylogeny of the genus Megalobrama
Article Snippet: .. The PCR components were the same as those mentioned above, and the conditions of the reaction were as follows: 94 °C for 5 min, followed by 30 cycles at 94 °C 30 sec, 56 °C for 30 sec, and 72 °C for 30 sec, and an extension at 72 °C for 5 min. After PCR, 2 µL of RE × 10 buffer, 0.2 µL acetylated BSA and 5 U Taq I (Promega Co., USA) was added to each sample (~1 µg), and the reaction was incubated at 65 °C for 2 h. Then, all digested samples of 7 populations (30 per population) were separated on 4% agarose gels with 0.5 µg/mL ethidium bromide. ..

Labeling:

Article Title: RNase P: role of distinct protein cofactors in tRNA substrate recognition and RNA-based catalysis
Article Snippet: Preparation of RNA and determination of 5′ end group of tRNA A plasmid that harbors the Schizosaccharomyces pombe precursor tRNASer (CUA), pSupS1 , which lacks 3′ CCA sequence, was linearized by Taq I and transcription was done with SP6 RNA polymerase (Promega) in the presence of 80 µCi of [α-32 P]UTP. .. The reaction products were separated in 8% polyacrylamide gel/7 M urea and the 110 nt labeled pSupS1 was excised from the gel, extracted and ethanol precipitated.

Purification:

Article Title: Effect of Temperature on Structure and Function of the Methanogenic Archaeal Community in an Anoxic Rice Field Soil
Article Snippet: .. Aliquots of the purified SSU rDNA were digested by Taq I (Promega, Mannheim, Germany). .. Each reaction tube contained 8 μl of the SSU rDNA amplicons, 1 μl of the appropriate incubation buffer supplied by the manufacturer (Promega), and 1 μl of restriction enzyme (10 U), made up to a total volume of 10 μl with deionized H2 O. Incubations were carried out in 0.5-ml reaction tubes for 3 h at 65°C.

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions. ..

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: The final purified PCR product of the gltA gene was then used for PCR-restriction fragment length polymorphism (PCR-RFLP) and further sequencing analyses. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

Sequencing:

Article Title: A Microbial Link between Elevated CO2 and Methane Emissions that is Plant Species-Specific
Article Snippet: An aliquot (16.8 μL) of the PCR products were digested with 5 U Taq I (Promega, Madison, WI), 1× buffer, and 1 μg bovine serum albumin for 2 h at 65 °C. .. The samples were sent to the University of Wisconsin-Madison Sequencing Facility for analysis using denaturing capillary electrophoresis on an ABI 3700 genetic analyzer (Applied Biosystems).

Article Title: RNase P: role of distinct protein cofactors in tRNA substrate recognition and RNA-based catalysis
Article Snippet: .. Preparation of RNA and determination of 5′ end group of tRNA A plasmid that harbors the Schizosaccharomyces pombe precursor tRNASer (CUA), pSupS1 , which lacks 3′ CCA sequence, was linearized by Taq I and transcription was done with SP6 RNA polymerase (Promega) in the presence of 80 µCi of [α-32 P]UTP. ..

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ]. .. The identities of these positive controls had been previously confirmed by PCR amplification, sequencing and BLASTN analysis of 18S rRNA gene.

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: A forward primer, which corresponded to the B. hyodysenteriae 23S rRNA gene sequence (GenBank #U72699) between 999th to 1022th nucleotides and a reverse primer between 1492th to 1515th nucleotides were used to amplify a 517 bp PCR product. .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions.

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: The final purified PCR product of the gltA gene was then used for PCR-restriction fragment length polymorphism (PCR-RFLP) and further sequencing analyses. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

Polyacrylamide Gel Electrophoresis:

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Article Snippet: PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures. .. The digestion and separation of the DNA fragments by 6% polyacrylamide gel electrophoresis were repeated at least three times for all serovars to establish the final restriction patterns.

IA:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Activated Clotting Time Assay:

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: The primer sequences were 5′-GGA ACA AAT TGG ACT TGG-3′ and 5′-TCT AGC ATG AAT CAC TCT GC-3′. .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA).

Plasmid Preparation:

Article Title: RNase P: role of distinct protein cofactors in tRNA substrate recognition and RNA-based catalysis
Article Snippet: .. Preparation of RNA and determination of 5′ end group of tRNA A plasmid that harbors the Schizosaccharomyces pombe precursor tRNASer (CUA), pSupS1 , which lacks 3′ CCA sequence, was linearized by Taq I and transcription was done with SP6 RNA polymerase (Promega) in the presence of 80 µCi of [α-32 P]UTP. ..

Software:

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: PCR-RFLP Using Gene Runner software (Hastings Software, USA), a 23S rRNA-specific primer set was designed and synthesized (Bionics, Korea). .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions.

Article Title: Serotonergic Regulation of Membrane Potential in Developing Rat Prefrontal Cortex: Coordinated Expression of 5-Hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 Receptors
Article Snippet: PCR primers were developed using PCGene software (Intelligenetics, Mountain View, CA). .. Restriction digest used Xho I for the 5-HT2A receptor and Taq I for the 5-HT7 receptor (Promega, Madison, WI).

Article Title: Characterization of the mitochondrial genome of Megalobrama terminalis in the Heilong River and a clearer phylogeny of the genus Megalobrama
Article Snippet: Specific SNP loci identification Single nucleotide polymorphism (SNP) loci of the complete mt genomes between MTH and other species from the genus Megalobrama were identified based on comparisons performed with the Clustal W method using the MegAlign program in DNASTAR Lasergene 7.1 software (DNASTAR Inc., Madison, WI, USA). .. The PCR components were the same as those mentioned above, and the conditions of the reaction were as follows: 94 °C for 5 min, followed by 30 cycles at 94 °C 30 sec, 56 °C for 30 sec, and 72 °C for 30 sec, and an extension at 72 °C for 5 min. After PCR, 2 µL of RE × 10 buffer, 0.2 µL acetylated BSA and 5 U Taq I (Promega Co., USA) was added to each sample (~1 µg), and the reaction was incubated at 65 °C for 2 h. Then, all digested samples of 7 populations (30 per population) were separated on 4% agarose gels with 0.5 µg/mL ethidium bromide.

Agarose Gel Electrophoresis:

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ). .. The digests were analyzed by electrophoresis on a 2% agarose gel in 1× Tris-borate-EDTA buffer.

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μl reaction volume on 2.0% (w/v) agarose gel.

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: The amplified ITS fragment was analyzed by 2.0% (w/v) agarose gel electrophoresis at 80 V in 0.5× TBE (45 mM Tris-borate, 1 mM EDTA, pH 8.0) buffer to check its intactness and absence of non-specific amplification. .. The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions.

Spectrophotometry:

Article Title: The Genomes of Four Meyerozyma caribbica Isolates and Novel Insights into the Meyerozyma guilliermondii Species Complex
Article Snippet: Quantity and quality of the recovered DNAs were checked by spectrophotometer and stored at −20°. .. The PCR product (4 μl) was digested with 5 U of Taq I (Promega, Madison, WI) in a 10 μl reaction volume at 65° for 2 hr, as per manufacturer’s instructions.

Staining:

Article Title: Prevalence of Bartonella henselae and Bartonella clarridgeiae in an Urban Indonesian Cat Population
Article Snippet: A panel of three restriction endonucleases was used as described in the manufacturer’s specifications in a 20-μl final volume: Hin fl, Mse I, and Taq I (Promega, Madison, Wis.) ( ). .. Gels were stained with ethidium bromide and visualized by UV fluorescence ( ).

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions. .. The gel was then stained in 0.5 μg/mL ethidium bromide solution for 30 min with rocking at 15 rpm on a platform rocker (Tarsons, Kolkata, India).

Article Title: Update on epidemiology of canine babesiosis in Southern France
Article Snippet: Amplified DNA fragments were separated by electrophoresis through 1.5 % agarose gels stained with ethidium bromide and visualized under ultraviolet light. .. Amplified fragments of 800-bp 18S rDNA (10 μL) were digested using Taq I (65 °C) and Hinf I (37 °C) enzymes (Promega, Madison, WI, USA) for 3 h according to a protocol adapted from Carret et al. [ ].

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA). .. The digested fragments were separated on Agarose gels stained with ethidium bromide and visualized under UV light.

Hood:

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: To minimize bacterial contamination in the laboratory, all isolations were performed in a safety class II hood cabinet. .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

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    Promega taq i
    PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, <t>Taq</t> I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).
    Taq I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).

    Journal: Journal of Clinical Microbiology

    Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

    doi:

    Figure Lengend Snippet: PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).

    Article Snippet: The amplified product of the gltA gene obtained with the set of primers suggested by Regnery et al. ( ) was digested with Taq I (Promega, Madison, Wis.) and Hha I (new England BioLabs, Beverly, Mass.) restriction endonucleases.

    Techniques: Polymerase Chain Reaction

    PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).

    Journal: Journal of Clinical Microbiology

    Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

    doi:

    Figure Lengend Snippet: PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).

    Article Snippet: The amplified product of the gltA gene obtained with the set of primers suggested by Regnery et al. ( ) was digested with Taq I (Promega, Madison, Wis.) and Hha I (new England BioLabs, Beverly, Mass.) restriction endonucleases.

    Techniques: Polymerase Chain Reaction

    Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

    Journal: Ecology and Evolution

    Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

    doi: 10.1002/ece3.639

    Figure Lengend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

    Article Snippet: Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ).

    Techniques: Selection

    Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

    Journal: Brazilian Journal of Microbiology

    Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)

    doi: 10.1590/S1517-838246220120018

    Figure Lengend Snippet: Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

    Article Snippet: PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures.

    Techniques: Construct, Generated

    PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California

    doi: 10.1128/JCM.39.4.1221-1226.2001

    Figure Lengend Snippet: PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

    Article Snippet: Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

    Techniques: Polymerase Chain Reaction