Structured Review

Promega taq i
PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, <t>Taq</t> I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).
Taq I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq i/product/Promega
Average 92 stars, based on 27 article reviews
Price from $9.99 to $1999.99
taq i - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California"

Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

Journal: Journal of Clinical Microbiology

doi:

PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).
Figure Legend Snippet: PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).

Techniques Used: Polymerase Chain Reaction

PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).
Figure Legend Snippet: PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).

Techniques Used: Polymerase Chain Reaction

2) Product Images from "Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States"

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

Journal: Ecology and Evolution

doi: 10.1002/ece3.639

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

3) Product Images from "Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)"

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)

Journal: Brazilian Journal of Microbiology

doi: 10.1590/S1517-838246220120018

Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.
Figure Legend Snippet: Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

Techniques Used: Construct, Generated

4) Product Images from "Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting"

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting

Journal: BMC Microbiology

doi: 10.1186/1471-2180-14-52

Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).
Figure Legend Snippet: Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).

Techniques Used: Sequencing, Polymerase Chain Reaction

5) Product Images from "Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California"

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.39.4.1221-1226.2001

PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .
Figure Legend Snippet: PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

Techniques Used: Polymerase Chain Reaction

6) Product Images from "Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer"

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer

Journal: Genetics and Molecular Biology

doi: 10.1590/S1415-47572012005000074

Genotype determination by using PCR-RFLP of the NAT2 gene fragment. Following PCR amplification separate digestions of each PCR product were carried out with the restriction enzymes Kpn I, Taq I, Bam H I and Alu I/ Msp I to detect the substitutions C481T, G590A, G857A and G191A, respectively. The different sizes of the digested products for each restriction enzyme which allow the individual’s genotype to be determined are shown diagrammatically.
Figure Legend Snippet: Genotype determination by using PCR-RFLP of the NAT2 gene fragment. Following PCR amplification separate digestions of each PCR product were carried out with the restriction enzymes Kpn I, Taq I, Bam H I and Alu I/ Msp I to detect the substitutions C481T, G590A, G857A and G191A, respectively. The different sizes of the digested products for each restriction enzyme which allow the individual’s genotype to be determined are shown diagrammatically.

Techniques Used: Polymerase Chain Reaction, Amplification

7) Product Images from "Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States"

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

Journal: Ecology and Evolution

doi: 10.1002/ece3.639

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.
Figure Legend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

Techniques Used: Selection

8) Product Images from "The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates"

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

Journal: Journal of Veterinary Science

doi: 10.4142/jvs.2006.7.3.277

PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.
Figure Legend Snippet: PCR-RFLP fragments of the 23S rRNA gene in 3% agarose gel electrophoresis digestion with Taq I. Lane M: 100 bp DNA ladder; lane 1: B. hyodysenteriae B204; lane 2: B. hyodysenteriae B234; lane 3: B. hyodysenteriae B169; lane 4: B. pilosicoli P43/6/78; lane 5 to 14: B. hyodysenteriae field isolates; lane 15: B. murdochii 56-150; lane 16: B. intermedia PWS/A; lane 17: B. innocens B256.

Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis

Related Articles

Polymerase Chain Reaction:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Article Snippet: .. PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures. .. To calculate the relative molecular masses of the digested fragments, a 100-bp DNA Ladder was used (Promega Co.).

Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting
Article Snippet: .. The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions. .. The restriction patterns were analyzed by electrophoresis of the 10 μL reaction volume on 2.0% (w/v) agarose gel in parallel with PCR 100 bp Low DNA ladder (Sigma-Aldrich) as molecular size standard.

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions. ..

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA). .. The digested fragments were separated on Agarose gels stained with ethidium bromide and visualized under UV light.

Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California
Article Snippet: .. Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene. .. Banding patterns were compared to the profiles of B. henselae Houston-1 (ATCC 49882), B. clarridgeiae (ATCC 700095), B. quintana (ATCC VR-358), B. bacilliformis (ATCC 35686), B. elizabethae (ATCC 49927), Bartonella strain cattle-1 (University of California, Davis), B. vinsonii subsp. vinsonii (ATCC VR-152), and B. vinsonii subsp. berkhoffii (ATCC 51672).

Incubation:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Amplification:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California
Article Snippet: .. The amplified product of the gltA gene obtained with the set of primers suggested by Regnery et al. ( ) was digested with Taq I (Promega, Madison, Wis.) and Hha I (new England BioLabs, Beverly, Mass.) restriction endonucleases. .. Taq I and Mse I (New England BioLabs) restriction endonucleases were utilized when using the set of primers suggested by Norman et al. ( ).

Article Title: Polymorphisms of arylamine N-acetyltransferase2 and risk of lung and colorectal cancer
Article Snippet: .. After amplification, PCR-products were digested with Kpn I (M1 allele), Taq I (M2 allele), Bam H I (M3 allele), and Msp I/Alu I (M4 allele) according to the protocol supplied by the manufacturer (Promega, USA). .. The digested fragments were separated on Agarose gels stained with ethidium bromide and visualized under UV light.

Size-exclusion Chromatography:

Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States
Article Snippet: .. Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ). .. Differences between the number of observed and expected genotypes at Crawfordsville, IA (historically known to be Z -race) and BENY colony individuals (pure E -race colony provided by Dr. C. Linn) were tested for significance with Chi-square (χ2 ) tests.

Purification:

Article Title: The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Article Snippet: .. Purified PCR products were digested with either Taq I or Alu I restriction enzymes (Promega, USA) according to the manufacturer's instructions. ..

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  • 92
    Promega taq i
    PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, <t>Taq</t> I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).
    Taq I, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq i/product/Promega
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    taq i - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).

    Journal: Journal of Clinical Microbiology

    Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

    doi:

    Figure Lengend Snippet: PCR (lanes 2 to 5) and PCR-RFLP (lanes 6 to 9, Taq I digestion; lanes 10 to 13, Hha I digestion) analyses of the gltA ). Lanes 1 and 14, standard 100-bp molecular ladder; lanes 2, 6, and 10, coyote isolates; lanes 3, 7, and 11, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 4, 8, and 12, B. vinsonii ATCC VR152; lanes 5, 9 and 13, B. henselae (strain U-4, University of California, Davis).

    Article Snippet: The amplified product of the gltA gene obtained with the set of primers suggested by Regnery et al. ( ) was digested with Taq I (Promega, Madison, Wis.) and Hha I (new England BioLabs, Beverly, Mass.) restriction endonucleases.

    Techniques: Polymerase Chain Reaction

    PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).

    Journal: Journal of Clinical Microbiology

    Article Title: Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology of Bartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

    doi:

    Figure Lengend Snippet: PCR-RFLP analysis (lanes 2 to 11, Taq I digestion; lanes 12 to 21, Mse I digestion) of the gltA ). Lanes 1 and 22, standard 100-bp molecular ladder; lanes 2 to 9 and 12 to 19, coyote isolates; lanes 10 and 20, B. vinsonii subsp. berkhoffii ATCC 51672; lanes 11 and 21, B. henselae (strain U-4; University of California, Davis).

    Article Snippet: The amplified product of the gltA gene obtained with the set of primers suggested by Regnery et al. ( ) was digested with Taq I (Promega, Madison, Wis.) and Hha I (new England BioLabs, Beverly, Mass.) restriction endonucleases.

    Techniques: Polymerase Chain Reaction

    Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

    Journal: Ecology and Evolution

    Article Title: Frequency of hybridization between Ostrinia nubilalis E-and Z-pheromone races in regions of sympatry within the United States

    doi: 10.1002/ece3.639

    Figure Lengend Snippet: Estimates of directional selection using Tajima's D by analysis of 100-bp sliding windows with 25-bp iterations across the Ostrinia nubilalis pheromone gland fatty-acyl reductase ( pgfar ) gene. This figure was adapted from a similar analysis by Lassance et al. ( 35 ) to show the location single nucleotide polymorphisms (SNPs) detected by markers at Taq I, Nde II, and Mse I restriction endonuclease sites.

    Article Snippet: Final PCR amplification took place with 32 cycles of 96°C for 20 sec, 52°C for 30 sec, and 72°C for 30 sec. PCR product in the entire reaction volume was digested with a restriction endonuclease (RE) by adding 2.0 μL 10× buffer and 0.5 U of Mse I (New England BioLabs, Ipswich, MA), or Nde II or Taq I (Promega) in a 20-μL total reaction volume , then incubated at 37°C (or 65°C for Taq I) for 14 h. Entire RE digest reaction volumes were loaded into 10-cm 3% agarose gels and separated at 80 V for 2 h. Resulting fragment sizes were estimated by comparison to a 50-bp ladder (Promega), and compared to those predicted for pgfar-e and pgfar-z alleles ( ).

    Techniques: Selection

    Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

    Journal: Brazilian Journal of Microbiology

    Article Title: Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)

    doi: 10.1590/S1517-838246220120018

    Figure Lengend Snippet: Dendrogram constructed by joint analysis of the bands generated by the restriction endonucleases Taq I, Tru 1I, Sau 3AI, and Msl I.

    Article Snippet: PCR products were subjected to restriction digestion with Taq I,Tru 1I, Sau 3AI and Msl I endonucleases (Promega Co.) for 3 h at the recommended temperatures.

    Techniques: Construct, Generated

    Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).

    Journal: BMC Microbiology

    Article Title: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting

    doi: 10.1186/1471-2180-14-52

    Figure Lengend Snippet: Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A : Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B : Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).

    Article Snippet: The PCR product (4 μL) was digested with 5 U of Taq I (Promega, Madison, USA) in a 10 μL reaction volume at 65°C as per manufacturer’s instructions.

    Techniques: Sequencing, Polymerase Chain Reaction