taq hotstartaq dna polymerase  (Qiagen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    HotStarTaq DNA Polymerase
    Description:
    For highly specific amplification with minimal optimization Kit contents Qiagen HotStarTaq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample Recombinant Enzyme Extra A Addition PCR Amplification Reaction Type High PCR Specificity For Highly Specific Amplification with Minimal Optimization Includes HotStarTaq DNA Polymerase 10x PCR Buffer 5x Q Solution 25mM MgCl2 Benefits Minimal optimization requirements High PCR specificity Easy handling and room temperature setup
    Catalog Number:
    203203
    Price:
    159
    Category:
    HotStarTaq DNA Polymerase
    Buy from Supplier


    Structured Review

    Qiagen taq hotstartaq dna polymerase
    HotStarTaq DNA Polymerase
    For highly specific amplification with minimal optimization Kit contents Qiagen HotStarTaq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample Recombinant Enzyme Extra A Addition PCR Amplification Reaction Type High PCR Specificity For Highly Specific Amplification with Minimal Optimization Includes HotStarTaq DNA Polymerase 10x PCR Buffer 5x Q Solution 25mM MgCl2 Benefits Minimal optimization requirements High PCR specificity Easy handling and room temperature setup
    https://www.bioz.com/result/taq hotstartaq dna polymerase/product/Qiagen
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    taq hotstartaq dna polymerase - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    pcr buffer
    dntp
    pcr

    Images

    Related Articles

    Amplification:

    Article Title: Small-Molecule Inhibition of HIV pre-mRNA Splicing as a Novel Antiretroviral Therapy to Overcome Drug Resistance
    Article Snippet: .. The mixture was aliquoted in a 96-well plate and subjected to PCR amplification using 0.375 U/15 μl of hotStarTaq DNA Polymerase with specific primers (0.3–0.6 μM) using the buffer provided by the manufacturer (QIAGEN). .. The PCR reaction was carried out in a GeneAmp 9700 PCR system.

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. The 16S rRNA sequences had an average substitution rate per base of 0.322% ± 0.277%, which represents a significant accuracy improvement to libraries amplified by HotStarTaq DNA polymerase (0.383% ± 0.275%) (T -test, P < 0.05). ..

    Next-Generation Sequencing:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    Hot Start PCR:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

    Polymerase Chain Reaction:

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C. .. Amplification success was assessed by agarose gel electrophoresis and the resulting products were pyrosequenced with the Pyromark Q24 System (Qiagen).

    Article Title: Small-Molecule Inhibition of HIV pre-mRNA Splicing as a Novel Antiretroviral Therapy to Overcome Drug Resistance
    Article Snippet: .. The mixture was aliquoted in a 96-well plate and subjected to PCR amplification using 0.375 U/15 μl of hotStarTaq DNA Polymerase with specific primers (0.3–0.6 μM) using the buffer provided by the manufacturer (QIAGEN). .. The PCR reaction was carried out in a GeneAmp 9700 PCR system.

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

    Generated:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    other:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: HotStarTaq DNA Polymerase, on the other hand, reached the highest MFI values.

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Sequencing:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    T-Test:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. The 16S rRNA sequences had an average substitution rate per base of 0.322% ± 0.277%, which represents a significant accuracy improvement to libraries amplified by HotStarTaq DNA polymerase (0.383% ± 0.275%) (T -test, P < 0.05). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen hotstart taq dna polymerase
    Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of <t>Taq</t> Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template <t>DNA.</t>
    Hotstart Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstart taq dna polymerase/product/Qiagen
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    hotstart taq dna polymerase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of Taq Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template DNA.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay

    doi: 10.1073/pnas.041619998

    Figure Lengend Snippet: Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of Taq Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template DNA.

    Article Snippet: The reaction mixture consisted of 67 mM Tris⋅HCl (pH 8.4) at 25°C, 16.6 mM ammonium sulfate, 2 mM magnesium chloride, 0.01% (vol/vol) Tween 20, 200 μM of each dNTP, 1 μM of each primer, and 0.5 units Hotstart Taq DNA polymerase (Qiagen) or AmpliTaq Gold (Perkin–Elmer).

    Techniques: Polymerase Chain Reaction, Fluorescence, Amplification, Produced