taq enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs taq enzyme
    Taq Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq enzyme/product/New England Biolabs
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    taq enzyme - by Bioz Stars, 2020-05
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Impaired telomerase activity hinders proliferation and in vitro transformation of Penaeus monodon lymphoid cells
    Article Snippet: .. Each precipitate was suspended in 50 µl PCR mixture consisting 5 µl 10× Taq Buffer, 0.2 mM each dNTP, 1 µM CX-ext primer, and 2 U Taq enzyme (New England Biolabs, Hitchin, Herts, UK). .. PCR was performed in Mastercycler personal™ (Eppendorf, Wesseling, Germany) and the programme used for the amplification was 95 °C for 5 min followed by 30 cycles of denaturation at 94 °C for 30 s, annealing at 50 °C for 30 s, extension at 72 °C for 60 s, followed by final extension at 72 °C for 10 min.

    Article Title: SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damage
    Article Snippet: .. The cDNA was subjected to standard PCR techniques using the Taq enzyme (New England Biolabs) and dNTPs from Invitrogen. ..

    Article Title: Spotted leaf11, a Negative Regulator of Plant Cell Death and Defense, Encodes a U-Box/Armadillo Repeat Protein Endowed with E3 Ubiquitin Ligase Activity W⃞
    Article Snippet: .. For PCR amplification of the Spl11 cDNA fragment, 1.5 μL of the diluted first strand cDNA was used in a 25-μL reaction volume with the Taq enzyme from New England Biolabs. .. Spl11- specific primers Uc-3 (5′-GATGCTTGCCTTATTGTCCTCA-3′) and Uc-4 (5′-ACGGATTGATATGCCTGACGAT-3′) were used for the amplification.

    Article Title: A Congenic Line of the C57BL/6J Mouse Strain that is Proficient in Melatonin Synthesis
    Article Snippet: .. PCR enzymes were either Taq enzyme (New England Biolabs, Ipswich, MA) or Takara LA Taq (Clontech Laboratories, Mountain View, CA). .. In more details, we first used a PCR method [ ] to amplify the genomic DNA and then directly sequence the PCR product to establish the genomic DNA sequence of the CBA alleles since this information was not readily available.

    Amplification:

    Article Title: Spotted leaf11, a Negative Regulator of Plant Cell Death and Defense, Encodes a U-Box/Armadillo Repeat Protein Endowed with E3 Ubiquitin Ligase Activity W⃞
    Article Snippet: .. For PCR amplification of the Spl11 cDNA fragment, 1.5 μL of the diluted first strand cDNA was used in a 25-μL reaction volume with the Taq enzyme from New England Biolabs. .. Spl11- specific primers Uc-3 (5′-GATGCTTGCCTTATTGTCCTCA-3′) and Uc-4 (5′-ACGGATTGATATGCCTGACGAT-3′) were used for the amplification.

    Fluorescence:

    Article Title: Disomic Inheritance and Segregation Distortion of SSR Markers in Two Populations of Cynodon dactylon (L.) Pers. var. dactylon
    Article Snippet: .. Each 10.5 μl reaction (one well) included 1.50 μl of 10 ng/ μl genomic DNA, 4.87 μl nuclease free water, 1.00 μl 1× standard Taq reaction buffer (10 mM Tris-HCl, 50 mM KCl and 1.5 mM MgCl2 ) (New England Biolabs Inc., MA, USA), 0.20 μl 10 mM dNTPs (Promega Corporation, WI, USA), 0.05 μl Taq enzyme (0.025 Units) (New England Biolabs Inc., MA, USA), 0.20 μl 1 μM IR-700 or -800 fluorescence labeled M 13 primer, and 1.34 μl each of 10 μM/μl forward and reverse primers. .. The PCR cycling was started with a denaturing step at 95°C for 5 min; followed by 14 cycles of 94°C for 20 s, 58°C 1 min, and 72°C 30 s; 28 cycles of 94°C for 20 s, 55°C 1 min, and 72°C 30 s; and a final extension at 72°C for 10 min, and then was stored at 4°C.

    Labeling:

    Article Title: Disomic Inheritance and Segregation Distortion of SSR Markers in Two Populations of Cynodon dactylon (L.) Pers. var. dactylon
    Article Snippet: .. Each 10.5 μl reaction (one well) included 1.50 μl of 10 ng/ μl genomic DNA, 4.87 μl nuclease free water, 1.00 μl 1× standard Taq reaction buffer (10 mM Tris-HCl, 50 mM KCl and 1.5 mM MgCl2 ) (New England Biolabs Inc., MA, USA), 0.20 μl 10 mM dNTPs (Promega Corporation, WI, USA), 0.05 μl Taq enzyme (0.025 Units) (New England Biolabs Inc., MA, USA), 0.20 μl 1 μM IR-700 or -800 fluorescence labeled M 13 primer, and 1.34 μl each of 10 μM/μl forward and reverse primers. .. The PCR cycling was started with a denaturing step at 95°C for 5 min; followed by 14 cycles of 94°C for 20 s, 58°C 1 min, and 72°C 30 s; 28 cycles of 94°C for 20 s, 55°C 1 min, and 72°C 30 s; and a final extension at 72°C for 10 min, and then was stored at 4°C.

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    New England Biolabs restriction enzyme taq i
    Restriction enzyme digestion patterns obtained with <t>Taq</t> I. Digestion mixtures were electrophoresed on 1.5% agarose gels. On each panel, the gel is flanked by a 100-bp ladder (Invitrogen Life Technologies) (lane MW). (A) The three species illustrated are unambiguously identified by the <t>Taq</t> I pattern. However, for the species illustrated in panel B, the Taq I patterns are similar to one another, although clearly distinct from that of the species in panel A.
    Restriction Enzyme Taq I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme taq i/product/New England Biolabs
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme taq i - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    New England Biolabs taq i enzyme
    Agarose gel electrophoresis for restriction fragment length polymorphism of VDR <t>Taq</t> I .
    Taq I Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq i enzyme/product/New England Biolabs
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    taq i enzyme - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    91
    New England Biolabs restriction enzymes taq i
    Schematic diagram of the RCCX module with Southern blotting and <t>Taq</t> I digestion analysis
    Restriction Enzymes Taq I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes taq i/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes taq i - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Restriction enzyme digestion patterns obtained with Taq I. Digestion mixtures were electrophoresed on 1.5% agarose gels. On each panel, the gel is flanked by a 100-bp ladder (Invitrogen Life Technologies) (lane MW). (A) The three species illustrated are unambiguously identified by the Taq I pattern. However, for the species illustrated in panel B, the Taq I patterns are similar to one another, although clearly distinct from that of the species in panel A.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection and Identification of Bartonella Species Pathogenic for Humans by PCR Amplification Targeting the Riboflavin Synthase Gene (ribC)

    doi: 10.1128/JCM.41.3.1069-1072.2003

    Figure Lengend Snippet: Restriction enzyme digestion patterns obtained with Taq I. Digestion mixtures were electrophoresed on 1.5% agarose gels. On each panel, the gel is flanked by a 100-bp ladder (Invitrogen Life Technologies) (lane MW). (A) The three species illustrated are unambiguously identified by the Taq I pattern. However, for the species illustrated in panel B, the Taq I patterns are similar to one another, although clearly distinct from that of the species in panel A.

    Article Snippet: Each reaction mixture in which amplicons were detected was subjected to digestion with the restriction enzyme Taq I (New England Biolabs).

    Techniques:

    Restriction fragment length polymorphism (RFLP) analysis of three generations of the clade C Symbiodinium -infected anemones. Amplified genomic fragments of small subunit ribosomal RNA genes (18S rDNA) from Symbiodinium were digested using the restriction enzymes Taq I and Sau 3A I (A and B, respectively). Lane 1 (control): Normal anemone harboring clade B Symbiodinium . Lanes 2–4: Generation 1 (G1) anemones. Lanes 5–7: Generation 2 (G2) anemones. Lanes 8–10: Generation 3 (G3) anemones. Ep, Exaiptasia pallida ; MW, molecular weight.

    Journal: PeerJ

    Article Title: Transmission of a heterologous clade C Symbiodinium in a model anemone infection system via asexual reproduction

    doi: 10.7717/peerj.2358

    Figure Lengend Snippet: Restriction fragment length polymorphism (RFLP) analysis of three generations of the clade C Symbiodinium -infected anemones. Amplified genomic fragments of small subunit ribosomal RNA genes (18S rDNA) from Symbiodinium were digested using the restriction enzymes Taq I and Sau 3A I (A and B, respectively). Lane 1 (control): Normal anemone harboring clade B Symbiodinium . Lanes 2–4: Generation 1 (G1) anemones. Lanes 5–7: Generation 2 (G2) anemones. Lanes 8–10: Generation 3 (G3) anemones. Ep, Exaiptasia pallida ; MW, molecular weight.

    Article Snippet: PCR products were purified by High Pure PCR Product Purification Kit (Roche, Germany) and then digested with either the restriction enzyme Taq I or Sau 3A I (New England Biolabs, USA) according to the manufacturer’s recommendations.

    Techniques: Infection, Amplification, Molecular Weight

    Agarose gel electrophoresis for restriction fragment length polymorphism of VDR Taq I .

    Journal: PLoS ONE

    Article Title: Serum 25-hydroxyvitamin D, serum calcium and vitamin D receptor (VDR) polymorphisms in a selected population with lumbar disc herniation—A case control study

    doi: 10.1371/journal.pone.0205841

    Figure Lengend Snippet: Agarose gel electrophoresis for restriction fragment length polymorphism of VDR Taq I .

    Article Snippet: Restriction enzyme digestion mixture for Taq I which consisted of 2 μL of 10X buffer (50 mM Potassium acetate, 20 mM Tris acetate, 10 mM Magnesium acetate, 100 μg/mL BSA, pH 7.9), 0.5 μL of Taq I enzyme (New England Bio Labs, UK), 5 μL of amplified PCR product (740 bp).

    Techniques: Agarose Gel Electrophoresis

    Schematic diagram of the RCCX module with Southern blotting and Taq I digestion analysis

    Journal: Clinical chemistry

    Article Title: Junction Site Analysis of Chimeric CYP21A1P/CYP21A2 Genes in 21-Hydroxylase Deficiency

    doi: 10.1373/clinchem.2011.174037

    Figure Lengend Snippet: Schematic diagram of the RCCX module with Southern blotting and Taq I digestion analysis

    Article Snippet: Restriction enzymes Taq I and Psh AI (New England Biolabs) were used to digest genomic DNA for Southern blotting.

    Techniques: Southern Blot