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Promega taq dnapol
Taq Dnapol, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq dnapol/product/Promega
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
taq dnapol - by Bioz Stars, 2020-04
85/100 stars

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Related Articles

Amplification:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: A cDNA segment of 1,806 nucleotides encompassing the M and E genes was amplified by polymerase chain reaction (PCR). .. The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI).

Agarose Gel Electrophoresis:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI). .. Products were verified by 0.8% agarose gel electrophoresis and then purified using the QIAquick PCR purification kit (Qiagen).

Cell Culture:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: Viral RNA (vRNA) was extracted from cell culture supernatant using a commercial silica-based method (QIAamp viral RNA, Qiagen, Valencia, CA). .. The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI).

Purification:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI). .. Products were verified by 0.8% agarose gel electrophoresis and then purified using the QIAquick PCR purification kit (Qiagen).

Polymerase Chain Reaction:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: .. The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI). .. Products were verified by 0.8% agarose gel electrophoresis and then purified using the QIAquick PCR purification kit (Qiagen).

Sequencing:

Article Title: Prolonged Co-circulation of Two Distinct Dengue Virus Type 3 Lineages in the Hyperendemic Area of Medell?n, Colombia
Article Snippet: The PCR mix included 1.75 mM MgCl, 0.25 mM dNTPs, 0.5 μM primers, and 0.05 U/μL Taq DNApol (Promega, Madison WI). .. Both strands of the amplicons were sequenced using appropriate primers and the Big Dye Terminator Cycle Sequencing System (ABI, Foster City, CA) following the protocols recommended by the manufacturer.

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  • 99
    Promega taq dna polymerase
    Superimposition of the thumb domains of <t>Taq</t> <t>DNA</t> polymerase (blue) with T7 DNA polymerase (pink). The arrows indicate the site of insertion of the T3 TBD (yellow). The primary amino acid sequence of Taq DNA polymerase from residue 470–507 is indicated below (blue) with the sequence of T3 TBD in yellow and the deleted region in red.
    Taq Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Promega
    Average 99 stars, based on 503 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Superimposition of the thumb domains of Taq DNA polymerase (blue) with T7 DNA polymerase (pink). The arrows indicate the site of insertion of the T3 TBD (yellow). The primary amino acid sequence of Taq DNA polymerase from residue 470–507 is indicated below (blue) with the sequence of T3 TBD in yellow and the deleted region in red.

    Journal: Nucleic Acids Research

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

    doi:

    Figure Lengend Snippet: Superimposition of the thumb domains of Taq DNA polymerase (blue) with T7 DNA polymerase (pink). The arrows indicate the site of insertion of the T3 TBD (yellow). The primary amino acid sequence of Taq DNA polymerase from residue 470–507 is indicated below (blue) with the sequence of T3 TBD in yellow and the deleted region in red.

    Article Snippet: A decrease of 4-fold in slippage peak was observed in DNA obtained from processive amplification using Taq DNA pol/TBD compared with Taq DNA polymerase.

    Techniques: Sequencing

    Slippage chromatograms were obtained from PCR products amplified with either Taq DNA polymerase or Taq DNA pol/TBD. One primer was labeled with 6-FAM fluorophore and the PCR product was digested with EcoRI. The DNA was gel purified and slippage polymorphisms detected using an automated DNA sequencer (model 377; Applied Biosystems) and GENESCAN 672 software. The result is one representative of three experiments.

    Journal: Nucleic Acids Research

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

    doi:

    Figure Lengend Snippet: Slippage chromatograms were obtained from PCR products amplified with either Taq DNA polymerase or Taq DNA pol/TBD. One primer was labeled with 6-FAM fluorophore and the PCR product was digested with EcoRI. The DNA was gel purified and slippage polymorphisms detected using an automated DNA sequencer (model 377; Applied Biosystems) and GENESCAN 672 software. The result is one representative of three experiments.

    Article Snippet: A decrease of 4-fold in slippage peak was observed in DNA obtained from processive amplification using Taq DNA pol/TBD compared with Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Amplification, Labeling, Purification, Software

    Streptavidin processivity assay. An immobilized single-stranded DNA molecule of 2000 nt in length was incubated in a reaction containing a primer hybridized to the 5′ end, and polymerase. Extension was initiated by the addition of dNTPs including [α- 32 P]dGTP, Mg 2+ and 0.8 mg/ml activated calf thymus DNA as described in Materials and Methods. Cleavage with restriction enzymes located 18, 96, 492, 1122 and 1898 nt, respectively, from the primer terminus only occurs if primer extension results in a double-stranded DNA substrate. Full extension with 5 U Promega Taq DNA polymerase in the absence of trap DNA allowed the percentage of primers extended to be determined.

    Journal: Nucleic Acids Research

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

    doi:

    Figure Lengend Snippet: Streptavidin processivity assay. An immobilized single-stranded DNA molecule of 2000 nt in length was incubated in a reaction containing a primer hybridized to the 5′ end, and polymerase. Extension was initiated by the addition of dNTPs including [α- 32 P]dGTP, Mg 2+ and 0.8 mg/ml activated calf thymus DNA as described in Materials and Methods. Cleavage with restriction enzymes located 18, 96, 492, 1122 and 1898 nt, respectively, from the primer terminus only occurs if primer extension results in a double-stranded DNA substrate. Full extension with 5 U Promega Taq DNA polymerase in the absence of trap DNA allowed the percentage of primers extended to be determined.

    Article Snippet: A decrease of 4-fold in slippage peak was observed in DNA obtained from processive amplification using Taq DNA pol/TBD compared with Taq DNA polymerase.

    Techniques: Incubation

    The effect of thioredoxin on processivity of the hybrid Taq DNA pol/TBD. ( A ) Extension assays were performed with a molar excess of template corresponding to a primer/template ratio of 470 for Taq DNA polymerase and Taq DNA polymerase (exo–) and 67 for Taq DNA pol/TBD and Taq DNA pol/TBD(exo–). Different ratios for the enzymes were used to ensure equal activity was loaded on the gel. (+) 100 µM thioredoxin, (–) no thioredoxin. No enzyme control shows the labeled primer alone. ( B ) Extension assay showing the effect of increasing concentrations of thioredoxin and enzyme dilution for Taq DNA pol/TBD(exo–). For each thioredoxin concentration (0.2, 2 and 20 µM), three enzyme concentrations were used (56, 28 and 5.6 pM) corresponding to a primer/template ratio of 67, 134 and 670.

    Journal: Nucleic Acids Research

    Article Title: Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase

    doi:

    Figure Lengend Snippet: The effect of thioredoxin on processivity of the hybrid Taq DNA pol/TBD. ( A ) Extension assays were performed with a molar excess of template corresponding to a primer/template ratio of 470 for Taq DNA polymerase and Taq DNA polymerase (exo–) and 67 for Taq DNA pol/TBD and Taq DNA pol/TBD(exo–). Different ratios for the enzymes were used to ensure equal activity was loaded on the gel. (+) 100 µM thioredoxin, (–) no thioredoxin. No enzyme control shows the labeled primer alone. ( B ) Extension assay showing the effect of increasing concentrations of thioredoxin and enzyme dilution for Taq DNA pol/TBD(exo–). For each thioredoxin concentration (0.2, 2 and 20 µM), three enzyme concentrations were used (56, 28 and 5.6 pM) corresponding to a primer/template ratio of 67, 134 and 670.

    Article Snippet: A decrease of 4-fold in slippage peak was observed in DNA obtained from processive amplification using Taq DNA pol/TBD compared with Taq DNA polymerase.

    Techniques: Activity Assay, Labeling, Concentration Assay

    Agarose gel electrophoresis of PCR-amplified DNA fragments of the pbp2B gene from S. pneumoniae . Lane M, molecular size marker (in base pairs). Lane 1, negative control; lane 2, penicillin-susceptible S. pneumoniae . Primer combinations are as follows: R1 + P5 + P6 (lane 3), R3 + P5 + P6 (lane 4), R1 + R3 + P5 + P6 (lane 5), R2 + P5 + P6 (lane 6), R4 + P5 + P6 (lane 7), and R2 + R4 + P5 + P6 (band C is poorly visible) (lane 8). (A) A 682-bp species-specific product arising from amplification with primers P5 and P6. (B) A 328- to 334-bp products arising from amplification with primers R1 to R3 and P6. (C) A 214-bp product arising from amplification with primers R4 and P6. (D) Amplification products produced as a result of annealing between a resistance product(s) and the 682-bp product and which are subsequently extended by Taq DNA polymerase to produce a larger product (±900 to 1,000 bp).

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Detection of Penicillin-Resistant Streptococcus pneumoniae in Cerebrospinal Fluid by a Seminested-PCR Strategy

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis of PCR-amplified DNA fragments of the pbp2B gene from S. pneumoniae . Lane M, molecular size marker (in base pairs). Lane 1, negative control; lane 2, penicillin-susceptible S. pneumoniae . Primer combinations are as follows: R1 + P5 + P6 (lane 3), R3 + P5 + P6 (lane 4), R1 + R3 + P5 + P6 (lane 5), R2 + P5 + P6 (lane 6), R4 + P5 + P6 (lane 7), and R2 + R4 + P5 + P6 (band C is poorly visible) (lane 8). (A) A 682-bp species-specific product arising from amplification with primers P5 and P6. (B) A 328- to 334-bp products arising from amplification with primers R1 to R3 and P6. (C) A 214-bp product arising from amplification with primers R4 and P6. (D) Amplification products produced as a result of annealing between a resistance product(s) and the 682-bp product and which are subsequently extended by Taq DNA polymerase to produce a larger product (±900 to 1,000 bp).

    Article Snippet: The 50-μl reaction mixture contained 0.5 μg of chromosomal DNA or 5 μl of boiled cells, 2 mM MgCl2 , 200 μM deoxynucleoside triphosphates (Boehringer Mannheim, Mannheim, Germany), 50 mM KCl, 10 mM Tris-HCl (pH 8), 1.0 μM (each) primer, and 2.5 U of Taq DNA polymerase (Promega Corp., Madison, Wis.).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Marker, Negative Control, Produced