taq dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taq dna polymerase
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. Eight clones were found to contain an identical insert of 3,003 nt for a partial but continuous open reading frame.

    Amplification:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: PCR amplification of the variable 3 (V3) region of the bacterial 16 S rRNA gene was performed on extracted DNA from each sample independently, as previously described . .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Article Title: Frequent In Vitro Recombination in Internal Transcribed Spacers 1 and 2 during Genotyping of Pneumocystis jirovecii ▿
    Article Snippet: .. The P. jirovecii DNA mixture (specimens 926 and 232) was amplified with Taq polymerase under standard conditions, and 5 μl of PCR II products was transferred to a new master mix containing 2.5 U Taq polymerase (Invitrogen, Carlsbad, CA), 200 μM each deoxynucleotide, 1.5 mM MgCl2 , 20 pmol each of primers FX and RT2, 1× PCR buffer, and MilliQ water in a total volume of 50 μl. ..

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: The GPx1 P198L and CAT C-262T genotypes were determined by polymerase chain reaction (PCR) amplification and restriction analysis according to the method of Kasznicki et al. ( ) with some modification. .. A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ).

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: For microbiome analyses, gastric aspirate DNA was amplified by Illumina MiSeq compatible bar-coded primers 27F/534R, targeting the 16s rRNA gene V1-V3 region. .. PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds.

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: PCR of 16 S rRNA The V8 and V9 regions of the 16 S rRNA gene were amplified using fusion primers containing 454 adaptor sequences ligated to the primers 1114F3- 5′YAACGARCGCRACCC and 1392R- 5′ACGGGCGGTGTGTRC . .. Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: First-strand cDNA (5' and 3'-RACE-Ready) was generated from ~ 1-μg of total RNA in a 10-μl volume by using the SMARTTM RACE cDNA amplification Kit (Clontech, Mountain View, CA). .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen).

    Article Title: Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand
    Article Snippet: .. The grinding solution was heated at 95°C for 10 minutes and then centrifuged at 14,000 rpm for 1 min. Wolbachia DNA was amplified in 20 μl mixture of 2 μl of 10x buffers, 1 μl of 50mM MgCl2 , 1 μl of 10 mM dNTP, 0.5 μl of forward and reverse primers, 1 μl of Taq DNA polymerase (Invitrogen, USA), and 12 μl of distilled water. .. PCR was carried out to 35 cycles at 95°C for 3 min, followed by 95°C for 1 min, 50°C for 1 min, and 72°C for 1 min. PCR products were electrophoresed on 2% agarose gel in TAE buffer strained with ethidium bromide.

    Article Title: Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst. Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst
    Article Snippet: Taq DNA polymerase (Thermo scientific Cat no: #EPO402) was dispensed into each reaction tube, and then, 2 μl of DNA sample was added to each sample. .. Agarose gel electrophoresis was first performed to control amplification of the PCR product, and then, the allele was detected after RFLP.

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: .. Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. The amplification conditions used were: 95°C 10 min, 95°C 30 s, 58°C 45 s and 72°C 50 s during 35 cycles, 72°C 5 min and finally 4°C (Veriti 96 Well thermal cycler, Applied Biosystems, United States).

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: .. DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA. .. The reactions were run in a Mastercycler Gradient™ (Eppendorf, Vienna, Austria) using the following program: initial denaturation of five minutes at 94 ºC, and 40 cycles of one minute at 94 ºC for melting, 30 seconds for primer annealing, and 45 seconds at 72 ºC for extension, and a final four minutes elongation at 72 ºC.

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: Mutation analysis - polymerase chain reaction - sequencing The seven exons of the GLA gene were amplified by polymerase chain reaction with specific primers and sequenced by the Sanger method on a Genetic Analyzer (Applied Biosystems Inc.). .. The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C.

    Positive Control:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. P. aeruginosa PAO1 was used as positive control in each one of the systems and Δ lasR/rhlR PAO1 as negative control of transcriptional regulators.

    TA Cloning:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. After TA-cloning into pGEM-T easy vector (Promega, Madison, WI), plasmid DNA was prepared by using the DNA Purification Kit (iNtRON Biotechnology, Kyunki-Do, Korea), and the inserts were sequenced by using the BigDye Terminator v3.1 Cycle Sequencing Kit on ABI Prism 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Construct:

    Article Title: Human Eb Peptide: Not just a By-product of Pre-pro-IGF1b Processing?
    Article Snippet: Paragraph title: Human Eb constructs ... PCR reaction was performed in a final volume of 20 µl containing: 1 µM of each oligonucleotide ( ); 0.6 mM MgCl2 , 1 × KCl buffer for Taq polymerase, 0.4 U of Taq polymerase (Fermentas International, Burlington, Canada), 2 mM of each dNTP and about 10 ng of human cDNA.

    Real-time Polymerase Chain Reaction:

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds. .. Cleaned amplicons were quantified by qPCR using KAPA Library Quantification Kit Illumana Platforms (Kapa Biosystems, Woburn, MA, USA).

    Multiplex Assay:

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Multiplex identifiers of 5–7 nucleotides were incorporated in the reverse primer sequence to allow for multiplexing. .. Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Incubation:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Additional enzymatic lysis was performed by adding 50 μl lysozyme (100 mg/ml) and 10 μl RNase A (10 mg/ml) and incubated for 1 hour at 37 °C, followed by the addition of 25 μl 25% sodium dodecyl sulfate, 25 μl proteinase K and 62.5 μl 5 M NaCl and was incubated for 1 hour at 65 °C. .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. A sufficient volume of transformed bacteria was plated onto LB agar plates containing 50 µg of ampicillin (Aspen, Deakin, Australia) per ml and an appropriate amount of Xgal (Promega, Madison, USA) and IPTG (Invitrogen, Carlsbad, USA), followed by overnight incubation at 37 °C.

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: At the end of the incubation, the sample was agitated in a vortex and the tip of the IsoCodeTM was removed. .. DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA.

    Activity Assay:

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. Ligase activity was then stopped by heating the reaction mix at 72 °C for 10 min. One µl of inactive ligation mix was then used to transform 50 µl of maximum efficiency E. coli (DH5α) competent cells (Invitrogen, Carlsbad, USA) using a MicroPulser (BIO-RAD, Hercules, USA).

    Cell Culture:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: DNA Extraction Pseudomonas aeruginosa strains were cultured in 5% sheep blood agar during 18 h at 37°C, and then one single colony was taken and lysed in an Eppendorf tube with 500 μL of TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) and were set into a heat block at 95°C for 5 min. .. Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States).

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA. .. Positive controls were DNA from cultured L. infantum from dog bone marrow.

    Modification:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl. .. 341 F and 518 R rRNA gene primers were modified to include adapter sequences specific to the Illumina technology and 6-base pair barcodes were used to allow multiplexing of samples as described previously .

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: The GPx1 P198L and CAT C-262T genotypes were determined by polymerase chain reaction (PCR) amplification and restriction analysis according to the method of Kasznicki et al. ( ) with some modification. .. A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ).

    Transformation Assay:

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. A sufficient volume of transformed bacteria was plated onto LB agar plates containing 50 µg of ampicillin (Aspen, Deakin, Australia) per ml and an appropriate amount of Xgal (Promega, Madison, USA) and IPTG (Invitrogen, Carlsbad, USA), followed by overnight incubation at 37 °C.

    Produced:

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. Two mock community samples extracted using the PowerSoil kit, two control BAL samples, and all of the NTCs produced no detectable amplification products.

    Sequencing:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Paragraph title: Maternal microbiota profiling: DNA extraction and 16S rRNA gene sequencing ... Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: Paragraph title: Microbial 16S rRNA gene sequencing ... PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds.

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Multiplex identifiers of 5–7 nucleotides were incorporated in the reverse primer sequence to allow for multiplexing. .. Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: On a sequence alignment of this predicted amino acid (aa) sequence with other known vertebrate Tert proteins generated several highly conserved domains. .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen).

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: Paragraph title: Mutation analysis - polymerase chain reaction - sequencing ... The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C.

    Ligation:

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. Ligase activity was then stopped by heating the reaction mix at 72 °C for 10 min. One µl of inactive ligation mix was then used to transform 50 µl of maximum efficiency E. coli (DH5α) competent cells (Invitrogen, Carlsbad, USA) using a MicroPulser (BIO-RAD, Hercules, USA).

    Genomic Sequencing:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: The genomic sequences encoding these domains were used to design primers TF and TR (Table ) for isolating a partial cDNA by reverse transcriptase-polymerase chain reaction (RT-PCR). .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen).

    Infection:

    Article Title: Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand
    Article Snippet: Each generation, randomly selected offspring were detected for Wolbachia infection. .. The grinding solution was heated at 95°C for 10 minutes and then centrifuged at 14,000 rpm for 1 min. Wolbachia DNA was amplified in 20 μl mixture of 2 μl of 10x buffers, 1 μl of 50mM MgCl2 , 1 μl of 10 mM dNTP, 0.5 μl of forward and reverse primers, 1 μl of Taq DNA polymerase (Invitrogen, USA), and 12 μl of distilled water.

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: The method dismisses additional DNA extraction and it has been used for human L. infantum infection identification . .. DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA.

    Generated:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: First-strand cDNA (5' and 3'-RACE-Ready) was generated from ~ 1-μg of total RNA in a 10-μl volume by using the SMARTTM RACE cDNA amplification Kit (Clontech, Mountain View, CA). .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen).

    DNA Sequencing:

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C. .. Polymerase chain reaction products were purified using the ZR-96 DNA Sequencing Clean-up Kit (Zymo Research Corp.), and the purified products were sequenced bidirectionally on an ABI 3130 capillary gel electrophoresis system (Applied Biosystems Inc.) according to the manufacturer’s protocol.

    Transmission Assay:

    Article Title: Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand
    Article Snippet: Similarly, their offspring were later checked for Wolbachia maternal transmission using PCR [ ]. .. The grinding solution was heated at 95°C for 10 minutes and then centrifuged at 14,000 rpm for 1 min. Wolbachia DNA was amplified in 20 μl mixture of 2 μl of 10x buffers, 1 μl of 50mM MgCl2 , 1 μl of 10 mM dNTP, 0.5 μl of forward and reverse primers, 1 μl of Taq DNA polymerase (Invitrogen, USA), and 12 μl of distilled water.

    Polymerase Chain Reaction:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: PCR amplification of the variable 3 (V3) region of the bacterial 16 S rRNA gene was performed on extracted DNA from each sample independently, as previously described . .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Article Title: Frequent In Vitro Recombination in Internal Transcribed Spacers 1 and 2 during Genotyping of Pneumocystis jirovecii ▿
    Article Snippet: .. The P. jirovecii DNA mixture (specimens 926 and 232) was amplified with Taq polymerase under standard conditions, and 5 μl of PCR II products was transferred to a new master mix containing 2.5 U Taq polymerase (Invitrogen, Carlsbad, CA), 200 μM each deoxynucleotide, 1.5 mM MgCl2 , 20 pmol each of primers FX and RT2, 1× PCR buffer, and MilliQ water in a total volume of 50 μl. ..

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: .. A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ). .. The PCR was performed as follows: pre-denaturation at 95°C for 5 min, followed by 33 (GPx1)/29 (CAT) cycles of 45 s at 95°C, 45 s at 66°C (GPx1)/70°C (CAT), and 45 s at 72°C, and ending with a single 7 min extension step at 72°C.

    Article Title: IAP Display: A Simple Method to Identify Mouse Strain Specific IAP Insertions
    Article Snippet: .. Display PCR 1 μl of secondary PCR reaction was added to 9 μl display PCR reactions containing 1× PCR buffer C (45 mM Tris-HCl pH 8.8, 11 mM NH4 SO4 , 0.9 mM MgCl2 , 6.7 mM β-mercaptoethanol, 113 μg/ml BSA (Ambion, Huntingdon UK), 0.25 mM dNTPs,), 0.9–1.9 pmol of γ32 P labelled RBIAPDP1 and 0.02 units/μl Taq DNA polymerase (ABgene, Epsom, UK). .. Reactions were cycled in a Perkin Elmer (Cambridge, UK) Cetus GeneAmp 9600 PCR machine using the following conditions: 96°C-2 min; 30× [96°C-30 s; 64°C-30 s; 72°C-30 s]; 72°C-10 min. All the controls from previous steps were carried forward in duplicate, and duplicate “display PCR DNA −ve controls” added.

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: .. PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds. .. Amplicons were individually identified by 2% agarose gel and cleaned by PureLink Quick PCR Purification kit (Invitrogen Corp., Carlsbad, CA, USA).

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Paragraph title: PCR of 16 S rRNA ... Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. The PCR product was separated on 1% agarose gels, documented using a UV transilluminator coupled with a CCD camera (Advanced American Biotechnology, Fullerton, CA) and recovered by using UltraCleanTM 15 DNA Purification Kit (MO BIO Laboratories, Carlsbad, CA).

    Article Title: Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand
    Article Snippet: Similarly, their offspring were later checked for Wolbachia maternal transmission using PCR [ ]. .. The grinding solution was heated at 95°C for 10 minutes and then centrifuged at 14,000 rpm for 1 min. Wolbachia DNA was amplified in 20 μl mixture of 2 μl of 10x buffers, 1 μl of 50mM MgCl2 , 1 μl of 10 mM dNTP, 0.5 μl of forward and reverse primers, 1 μl of Taq DNA polymerase (Invitrogen, USA), and 12 μl of distilled water.

    Article Title: Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst. Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst
    Article Snippet: The PCR was carried out in a volume of 20 μl containing 50 ng of DNA, 16 pmol of each primer, 200 μM of each dNTP, 50 mM KCl, 10 mM Tris‐HCl (pH 8.3), 2.0 mM MgCl2 , and 1.0 U AmpliTaq Gold (Applied Biosystems). .. Taq DNA polymerase (Thermo scientific Cat no: #EPO402) was dispensed into each reaction tube, and then, 2 μl of DNA sample was added to each sample.

    Article Title: The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study
    Article Snippet: .. For PCR, 25 μl reaction mixture contained as follows: 2.5 μl of 10× reaction buffer (with 1.5 mM MgCl2 ), 2 μl of deoxynucleotide triphosphate (dNTP; 2.5 mM), 2 μl of each pair primer, 50 ng DNA template, 1 μl of 0.4U Taq polymerase (Applied Biosystems, Evry, France) and 14.5 μl ddH2 O. ..

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: .. Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. Two µl of treated cDNAs was then ligated with 25 ng of pGEM-T plasmid vector (Promega, Madison, USA) using T4 DNA ligase in an overnight reaction at 16 °C.

    Article Title: Human Eb Peptide: Not just a By-product of Pre-pro-IGF1b Processing?
    Article Snippet: .. PCR reaction was performed in a final volume of 20 µl containing: 1 µM of each oligonucleotide ( ); 0.6 mM MgCl2 , 1 × KCl buffer for Taq polymerase, 0.4 U of Taq polymerase (Fermentas International, Burlington, Canada), 2 mM of each dNTP and about 10 ng of human cDNA. .. All PCR reactions were performed in Biometra T-gradient thermocycler under the following conditions: (1) predenaturation at 95 °C for 5 min, (2) denaturation at 95 °C for 30 s, (3) annealing at 55 °C for 30 s, (4) elongation at 72 °C for 30 s, and (5) final elongation at 72 °C for 7 min. Steps 2–4 were repeated 25 times.

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: Paragraph title: Polymerase chain reaction (PCR) ... DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA.

    Binding Assay:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: The aqueous phase was removed and combined with 200 μl DNA binding buffer (Zymo Research, Irvine, CA, USA). .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Multiplexing:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl. .. 341 F and 518 R rRNA gene primers were modified to include adapter sequences specific to the Illumina technology and 6-base pair barcodes were used to allow multiplexing of samples as described previously .

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Multiplex identifiers of 5–7 nucleotides were incorporated in the reverse primer sequence to allow for multiplexing. .. Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst. Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst
    Article Snippet: ELP4 genotype was determined by PCR, endonuclease restriction analysis, and gel electrophoresis. .. Taq DNA polymerase (Thermo scientific Cat no: #EPO402) was dispensed into each reaction tube, and then, 2 μl of DNA sample was added to each sample.

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C. .. Polymerase chain reaction products were purified using the ZR-96 DNA Sequencing Clean-up Kit (Zymo Research Corp.), and the purified products were sequenced bidirectionally on an ABI 3130 capillary gel electrophoresis system (Applied Biosystems Inc.) according to the manufacturer’s protocol.

    Irradiation:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl. .. 341 F and 518 R rRNA gene primers were modified to include adapter sequences specific to the Illumina technology and 6-base pair barcodes were used to allow multiplexing of samples as described previously .

    Mutagenesis:

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: Paragraph title: Mutation analysis - polymerase chain reaction - sequencing ... The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C.

    Isolation:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: Paragraph title: Gene identification and isolation ... RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen).

    Size-exclusion Chromatography:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. The PCR product was separated on 1% agarose gels, documented using a UV transilluminator coupled with a CCD camera (Advanced American Biotechnology, Fullerton, CA) and recovered by using UltraCleanTM 15 DNA Purification Kit (MO BIO Laboratories, Carlsbad, CA).

    Purification:

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds. .. Amplicons were individually identified by 2% agarose gel and cleaned by PureLink Quick PCR Purification kit (Invitrogen Corp., Carlsbad, CA, USA).

    Article Title: Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
    Article Snippet: Each contained 10–15 µL (mock community and water samples) or 5 uL (BAL samples) of template DNA, 5 uL of 10× buffer (Invitrogen, Carlsbad, CA, USA), 1 µL of 10 mM dNTP mix (Invitrogen, Carlsbad, CA, USA), 1.5 µL BSAI (Fermentas, CA, USA), 1.5 µL 50 mM MgCl2 (Invitrogen, Carlsbad, CA, USA), 1 µL of each 10 uM primer, and 1 unit of Taq Polymerase (Invitrogen, Carlsbad, CA, USA). .. Following amplification, PCR products for each sample were pooled and purified using the QIAquick PCR Purification Kit and quantitated using the Qbit Fluorimeter (Invitrogen, Carlsbad, CA, USA).

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C. .. Polymerase chain reaction products were purified using the ZR-96 DNA Sequencing Clean-up Kit (Zymo Research Corp.), and the purified products were sequenced bidirectionally on an ABI 3130 capillary gel electrophoresis system (Applied Biosystems Inc.) according to the manufacturer’s protocol.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: .. RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. The PCR product was separated on 1% agarose gels, documented using a UV transilluminator coupled with a CCD camera (Advanced American Biotechnology, Fullerton, CA) and recovered by using UltraCleanTM 15 DNA Purification Kit (MO BIO Laboratories, Carlsbad, CA).

    Blocking Assay:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: DNA Extraction Pseudomonas aeruginosa strains were cultured in 5% sheep blood agar during 18 h at 37°C, and then one single colony was taken and lysed in an Eppendorf tube with 500 μL of TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) and were set into a heat block at 95°C for 5 min. .. Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States).

    Staining:

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ). .. The products were analyzed by electrophoresis on a 2.5 or 3.5% agarose gel and visualized by staining with Genecolour fluorescent dye.

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. Amplification products were loaded into a 1% agarose gel stained with SYBR® green I (S7567, Life Technologies, United States) and visualized with Gel DOCTM XR + with Image LabTM software (Bio-Rad, United States).

    Nested PCR:

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: .. Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. Two µl of treated cDNAs was then ligated with 25 ng of pGEM-T plasmid vector (Promega, Madison, USA) using T4 DNA ligase in an overnight reaction at 16 °C.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: The GPx1 P198L and CAT C-262T genotypes were determined by polymerase chain reaction (PCR) amplification and restriction analysis according to the method of Kasznicki et al. ( ) with some modification. .. A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ).

    Plasmid Preparation:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. After TA-cloning into pGEM-T easy vector (Promega, Madison, WI), plasmid DNA was prepared by using the DNA Purification Kit (iNtRON Biotechnology, Kyunki-Do, Korea), and the inserts were sequenced by using the BigDye Terminator v3.1 Cycle Sequencing Kit on ABI Prism 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA).

    Article Title: Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice
    Article Snippet: Preparation of subtracted cDNA libraries Subtracted cDNAs (the nested PCR products described in the previous section) were treated with Taq DNA polymerase for 10 min at 72 °C in a reaction mix of 20 µl containing 17 µl of nested PCR products, 2 µl of PCR buffer (×10), 0.5 µl of MgCl2 , 0.25 µl of dNTP mix, and 0.25 µl of Taq DNA polymerase (Invitrogen, California, USA). .. Two µl of treated cDNAs was then ligated with 25 ng of pGEM-T plasmid vector (Promega, Madison, USA) using T4 DNA ligase in an overnight reaction at 16 °C.

    Software:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. Amplification products were loaded into a 1% agarose gel stained with SYBR® green I (S7567, Life Technologies, United States) and visualized with Gel DOCTM XR + with Image LabTM software (Bio-Rad, United States).

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: Results were analyzed using the software SeqScape 2.5.0 (Applied Biosystems Inc.). .. The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C.

    SYBR Green Assay:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. Amplification products were loaded into a 1% agarose gel stained with SYBR® green I (S7567, Life Technologies, United States) and visualized with Gel DOCTM XR + with Image LabTM software (Bio-Rad, United States).

    Negative Control:

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. P. aeruginosa PAO1 was used as positive control in each one of the systems and Δ lasR/rhlR PAO1 as negative control of transcriptional regulators.

    Agarose Gel Electrophoresis:

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ). .. The products were analyzed by electrophoresis on a 2.5 or 3.5% agarose gel and visualized by staining with Genecolour fluorescent dye.

    Article Title: Initial microbial community of the neonatal stomach immediately after birth
    Article Snippet: PCR reactions were performed with Taq DNA Polymerase (Invitrogen Corp., Carlsbad, CA, USA) under the following condition: initial melting step at 94°C for 2 minutes, followed by 30 cycles of 94°C for 20°seconds, 56°C for 30 seconds, and 72°C for 45°seconds. .. Amplicons were individually identified by 2% agarose gel and cleaned by PureLink Quick PCR Purification kit (Invitrogen Corp., Carlsbad, CA, USA).

    Article Title: Combined sterile insect technique and incompatible insect technique: The first proof-of-concept to suppress Aedes aegypti vector populations in semi-rural settings in Thailand
    Article Snippet: The grinding solution was heated at 95°C for 10 minutes and then centrifuged at 14,000 rpm for 1 min. Wolbachia DNA was amplified in 20 μl mixture of 2 μl of 10x buffers, 1 μl of 50mM MgCl2 , 1 μl of 10 mM dNTP, 0.5 μl of forward and reverse primers, 1 μl of Taq DNA polymerase (Invitrogen, USA), and 12 μl of distilled water. .. PCR was carried out to 35 cycles at 95°C for 3 min, followed by 95°C for 1 min, 50°C for 1 min, and 72°C for 1 min. PCR products were electrophoresed on 2% agarose gel in TAE buffer strained with ethidium bromide.

    Article Title: The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study
    Article Snippet: For PCR, 25 μl reaction mixture contained as follows: 2.5 μl of 10× reaction buffer (with 1.5 mM MgCl2 ), 2 μl of deoxynucleotide triphosphate (dNTP; 2.5 mM), 2 μl of each pair primer, 50 ng DNA template, 1 μl of 0.4U Taq polymerase (Applied Biosystems, Evry, France) and 14.5 μl ddH2 O. .. The PCR products were analyzed by horizontal electrophoresis on an ethidium bromide-stained agarose gel (2% w/v) and then photographed.

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States). .. Amplification products were loaded into a 1% agarose gel stained with SYBR® green I (S7567, Life Technologies, United States) and visualized with Gel DOCTM XR + with Image LabTM software (Bio-Rad, United States).

    Article Title: The Prevalence of Fabry Disease Among Turkish Patients with Non-Obstructive Hypertrophic Cardiomyopathy: Insights from a Screening Study
    Article Snippet: The polymerase chain reaction amplifications were carried out using Taq DNA polymerase (PhireII HS, Thermo Inc.) and a polymerase chain reaction protocol with an initial hold of 1 minutes at 95 °C, 45 cycles (of 10 seconds at 95 °C, 10 seconds at 60 °C, and 20 seconds at 72 °C), and then a final extension of 1 minute at 72 °C. .. After the thermal cycle protocol for polymerase chain reaction, the product was checked using 2% agarose gel electrophoresis.

    Electrophoresis:

    Article Title: Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women
    Article Snippet: A total volume of 25 μl containing 2.5 μl of 10X PCR buffer, 200 μM each of dNTP, 1.5 mM MgCl2 , and 0.75 U of Taq polymerase (Thermo Fisher Scientific Inc. Vilnius, Lithuania), 1.5 μl of genomic DNA template (about 30–80 ng), 0.30 μM of each primer: forward 5′-GTGCCCCTACGCAGGTACAG-3′ and reverse 5′-GGACATACACACAGTTCTGCTGAC-3′ for the P198L genotype (designed by Primer-BLAST); forward 5′-CTGATAACCGGGAGCCCCGCCCTGGGTTCGGATAT-3′ and reverse 5′-CTAGGCAGGCCAAGATTGGAAGCCCAATGG-3′ for the C-262T genotype were used ( ). .. The products were analyzed by electrophoresis on a 2.5 or 3.5% agarose gel and visualized by staining with Genecolour fluorescent dye.

    Article Title: The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study
    Article Snippet: For PCR, 25 μl reaction mixture contained as follows: 2.5 μl of 10× reaction buffer (with 1.5 mM MgCl2 ), 2 μl of deoxynucleotide triphosphate (dNTP; 2.5 mM), 2 μl of each pair primer, 50 ng DNA template, 1 μl of 0.4U Taq polymerase (Applied Biosystems, Evry, France) and 14.5 μl ddH2 O. .. The PCR products were analyzed by horizontal electrophoresis on an ethidium bromide-stained agarose gel (2% w/v) and then photographed.

    Sampling:

    Article Title: The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study
    Article Snippet: Paragraph title: Blood sampling and genotyping ... For PCR, 25 μl reaction mixture contained as follows: 2.5 μl of 10× reaction buffer (with 1.5 mM MgCl2 ), 2 μl of deoxynucleotide triphosphate (dNTP; 2.5 mM), 2 μl of each pair primer, 50 ng DNA template, 1 μl of 0.4U Taq polymerase (Applied Biosystems, Evry, France) and 14.5 μl ddH2 O.

    DNA Extraction:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Paragraph title: Maternal microbiota profiling: DNA extraction and 16S rRNA gene sequencing ... Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Article Title: Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst. Association between ELP4 rs986527 polymorphism and the occurrence and development of intracranial arachnoid cyst
    Article Snippet: 2.2.3 ELP4 rs986527 polymorphism genotyping DNA was extracted from blood using the Puregene DNA Isolation Kit (Gentra). .. Taq DNA polymerase (Thermo scientific Cat no: #EPO402) was dispensed into each reaction tube, and then, 2 μl of DNA sample was added to each sample.

    Article Title: AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains of Pseudomonas aeruginosa Isolated From Burned Patients
    Article Snippet: Paragraph title: DNA Extraction ... Genes related to Las and Rhl systems were amplified ( ) in a final volume of 25 μL of buffer 1X, 3 mM MgCl2 , 200 μM dNTP′s, 0.2 μM primer forward and reverse, 0.026 U/μL Taq polymerase (Amplitaq Gold® DNA Polymerase, Applied Biosystems N808-0241, United States).

    Article Title: The performance of serological tests for Leishmaniainfantum infection screening in dogs depends on the prevalence of the disease
    Article Snippet: The method dismisses additional DNA extraction and it has been used for human L. infantum infection identification . .. DNA amplification was performed in a final volume of 25 µL containing 2.5 µL of Taq polymerase 10 x buffer (160 mM (NH4 )2 SO4 , 670 mM Tris-HCl pH 8.8, and 0.1% Tween-20); 1.5 mM MgCl2 ; 100 µM of dATP, dCTP, dGTP and dTTP; 0.2 µM of each primer; 0.5 units of Taq polymerase (Platinum™ Taq DNA Polymerase, Invitrogen, Carlsbad, CA, EUA); and 5 µL of DNA.

    Concentration Assay:

    Article Title: The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study
    Article Snippet: The quality and concentration of extracted DNA was measured in two OD wavelength 260 and 280 nm using NanoDrop (Thermo Scientific, U.S.A.). .. For PCR, 25 μl reaction mixture contained as follows: 2.5 μl of 10× reaction buffer (with 1.5 mM MgCl2 ), 2 μl of deoxynucleotide triphosphate (dNTP; 2.5 mM), 2 μl of each pair primer, 50 ng DNA template, 1 μl of 0.4U Taq polymerase (Applied Biosystems, Evry, France) and 14.5 μl ddH2 O.

    DNA Purification:

    Article Title: Medaka tert produces multiple variants with differential expression during differentiation in vitro and in vivo
    Article Snippet: RT-PCR was run in 25 μl of 1 X PCR buffer containing 2 mM MgCl2 , 0.2 μM of each primer, 200 μM dNTPs each, 50-200 ng cDNA template and 1 U Taq DNA polymerase for 35 cycles (94°C 30 s, 60°C 30 s and 72°C 3 min 10 sec at 94ºC, 10 sec at 53ºC, and 3 min at 72ºC for 35 cycles, followed by 10 min at 72ºC with Taq DNA polymerase (Invitrogen). .. The PCR product was separated on 1% agarose gels, documented using a UV transilluminator coupled with a CCD camera (Advanced American Biotechnology, Fullerton, CA) and recovered by using UltraCleanTM 15 DNA Purification Kit (MO BIO Laboratories, Carlsbad, CA).

    Lysis:

    Article Title: Obesity during pregnancy results in maternal intestinal inflammation, placental hypoxia, and alters fetal glucose metabolism at mid-gestation
    Article Snippet: Additional enzymatic lysis was performed by adding 50 μl lysozyme (100 mg/ml) and 10 μl RNase A (10 mg/ml) and incubated for 1 hour at 37 °C, followed by the addition of 25 μl 25% sodium dodecyl sulfate, 25 μl proteinase K and 62.5 μl 5 M NaCl and was incubated for 1 hour at 65 °C. .. Briefly, each reaction contained 5 pmol of primer, 200 mM of dNTPs, 1.5 μl 50 mM MgCl2 , 2 μl of 10 mg/ml bovine serum albumin (irradiated with a transilluminator to eliminate contaminating DNA) and 0.25 μl Taq DNA polymerase (Life Technologies, Canada) for a total reaction volume of 50 μl.

    Variant Assay:

    Article Title: Human Eb Peptide: Not just a By-product of Pre-pro-IGF1b Processing?
    Article Snippet: The term hEb used in the current paper correspond to exon 4/5 splice variant, unless otherwise stated. .. PCR reaction was performed in a final volume of 20 µl containing: 1 µM of each oligonucleotide ( ); 0.6 mM MgCl2 , 1 × KCl buffer for Taq polymerase, 0.4 U of Taq polymerase (Fermentas International, Burlington, Canada), 2 mM of each dNTP and about 10 ng of human cDNA.

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  • 90
    Thermo Fisher taq dna polymerase
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    taq dna polymerase - by Bioz Stars, 2020-01
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