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TaKaRa taq dna polymerase
Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 280 article reviews
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taq dna polymerase - by Bioz Stars, 2020-01
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Related Articles

Clone Assay:

Article Title: Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
Article Snippet: P . pastoris strain KM71 was grown in YPD broth (2% tryptone, 1% yeast extract, 2% dextrose) at 28 °C, or BMMY broth (1% yeast extract, 1.34% YNB, 4 × 10−5 % biotin, 2% dextrose, 100 mM potassium buffer, 0.5% methanol) at 28 °C, or MD broth plates (2% dextrose, 4 × 10−5 % biotin, 1.34% YNB and 2% agar) at 28 °C for screening yeast recombinants, or tributyrin agar plate (2% tryptone, 1% yeast extract, 1.34% YNB, 100 mM potassium buffer, 4 × 10−5 % biotin, 1% glycerol at 16 °C, 1% tributyrin and 2% agar) at 28 °C. pMD19-T cloning vector (Takara, Otsu, Japan), pET-28a (Novagen, Darmstadt, Germany) and the plasmid pPIC9K (Invitrogen, Carlsbad, USA) were applied for constructing the expression plasmids. .. Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan).

Amplification:

Article Title: A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis
Article Snippet: The final optimized concentration of universal primer (UP) was 500 nmol L−1 in both singlet PCR and UP-M-PCR, which is the same as in normal singlet PCR, while the compound specific primers were 25 nmol L−1 (about 1/20 of UP) in singlet PCR that can ensure an efficient amplification for all these primers, but in fifteen-plex UP-M-PCR, because of the interaction and the difference of work efficiency among primers, all compound specific primers at 25 nmol L−1 could not get an equivalent amount of amplified products, thus there need an adjustment. .. To test the efficiency of Taq Polymerase to be employed in PCR assays, comparative tests were made with several Taq polymerases, such as Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase, and TaKaRa Taq ™.

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: Paragraph title: Polymerase chain reaction (PCR) amplification of LukS-I and LukF-I ... PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes.

Article Title: Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes
Article Snippet: .. One microgram of total RNA was converted to cDNA by superscript reverse transcriptase and then amplified by a Taq polymerase using reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. The relative expression of iNOS, COX-2, filaggrin, and SPT were analyzed using PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control.

Article Title: Genetic Variations in ABCG2 Gene Predict Breast Carcinoma Susceptibility and Clinical Outcomes after Treatment with Anthracycline-Based Chemotherapy
Article Snippet: .. PCR amplification was performed as follows: 100 ng of genomic DNA, 300 nM of each primer, 200 nM dNTPs, and 0.5 U Taq polymerase in PCR buffer (TaKaRa Biotechnology (Dalian) Co. Ltd., Dalian, China). .. The reaction for amplification was carried out in the following conditions: an initial melting step of 5 min at 94°C, followed by 35 cycles of 30 s at 94°C, 30 s at 58°C, and 1 min at 72°C and a final elongation of 5 min at 72°C.

Article Title: Clinical verification of plasma messenger RNA as novel noninvasive biomarker identified through bioinformatics analysis for lung cancer
Article Snippet: .. The target sequences were amplified by PCR in 50μl of 1×Taq buffer containing 0.3μM of each primer, 1.5 mM magnesium chloride, 200μM dNTP mixture, 2.5 units of Taq polymerase and 1μl (10μg) of each cDNA by Ex Taq PCR kit (TAKARA cat#RR001A). ..

Article Title: 2,5-Dihydroxyacetophenone Induces Apoptosis of Multiple Myeloma Cells by Regulating the MAPK Activation Pathway
Article Snippet: .. One microgram of total RNA was changed to cDNA via reverse transcriptase, then amplified with a Taq polymerase via the use of an RT-PCR kit (Takara Bio Inc., Tokyo, Japan). .. The relative expressions of Bcl-xl , Bcl-2 , Cyclin D1 , Survivin , MMP-9 , COX-2 , Bax , and p21 were analyzed using a TaKaRa PCR Thermal Cycler (Code TP350, Takara Bio Inc., Tokyo, Japan) with glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) as an internal control.

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: Briefly, total RNA was first reverse transcribed into cDNA using Superscript II reverse transcriptase (Life Technologies) and then amplified in a programmable Applied Biosystems 2720 thermal cycler (Singapore). .. For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720.

Single-particle Tracking:

Article Title: Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes
Article Snippet: One microgram of total RNA was converted to cDNA by superscript reverse transcriptase and then amplified by a Taq polymerase using reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. The relative expression of iNOS, COX-2, filaggrin, and SPT were analyzed using PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control.

Synthesized:

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: The synthesized cDNA was stored at −20°C. .. The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.).

Article Title: Selection of Aptamers Specific for Adipose Tissue
Article Snippet: Library and aptamers were synthesized on a 3400 DNA/RNA synthesizer (Applied Biosystems, Foster City, CA). .. Taq-polymerase, dNTPs and other reagents were purchased from Takara.

Quantitative RT-PCR:

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: Paragraph title: Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ... The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.).

Real-time Polymerase Chain Reaction:

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: The qPCR assays were performed on an ABI 7500 FAST Real-Time System (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.).

Article Title: Anti-myeloma Effects of Icariin Are Mediated Through the Attenuation of JAK/STAT3-Dependent Signaling Cascade
Article Snippet: Then RNA was Reverse transcribed into cDNA using superscript reverse transcriptase and Taq polymerase by reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. For quantitative PCR analyses, Bcl-2, Bcl-xl, and Survivin were polymerized using the following primers: Bcl-2, 5′-TTGTGGCCTTCTTTGAGTTCGGTG-3′ and 5′-TACAGTTCCACAAAGGCATCCCAG-3′.

Microarray:

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: To confirm the differential gene expression of laryngeal cancer revealed during cDNA microarray analysis, we used a 2-step method of semi-quantitative RT-PCR starting with tissues from 32 cases of laryngeal cancer and matched normal adjacent tissues. .. For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720.

Incubation:

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: Subsequently, 10 µM deoxynucleotide triphosphate (1 µl), 0.1 M dithiothreitol (2 µl), 5× RT buffer (4 µl) and reverse transcriptase (1 µl) were added (all from Takara Biotechnology Co., Ltd., Dalian, China), followed by incubation at 50°C for 50 min in water bath. .. The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.).

Expressing:

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes. .. All ORFs were amplified without a histidine tag because pETBlue-2 allowed T7lac promoter-based expression of target genes with C-terminal histidine tagged sequences.

Article Title: Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
Article Snippet: P . pastoris strain KM71 was grown in YPD broth (2% tryptone, 1% yeast extract, 2% dextrose) at 28 °C, or BMMY broth (1% yeast extract, 1.34% YNB, 4 × 10−5 % biotin, 2% dextrose, 100 mM potassium buffer, 0.5% methanol) at 28 °C, or MD broth plates (2% dextrose, 4 × 10−5 % biotin, 1.34% YNB and 2% agar) at 28 °C for screening yeast recombinants, or tributyrin agar plate (2% tryptone, 1% yeast extract, 1.34% YNB, 100 mM potassium buffer, 4 × 10−5 % biotin, 1% glycerol at 16 °C, 1% tributyrin and 2% agar) at 28 °C. pMD19-T cloning vector (Takara, Otsu, Japan), pET-28a (Novagen, Darmstadt, Germany) and the plasmid pPIC9K (Invitrogen, Carlsbad, USA) were applied for constructing the expression plasmids. .. Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan).

Article Title: Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes
Article Snippet: One microgram of total RNA was converted to cDNA by superscript reverse transcriptase and then amplified by a Taq polymerase using reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. The relative expression of iNOS, COX-2, filaggrin, and SPT were analyzed using PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control.

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: To confirm the differential gene expression of laryngeal cancer revealed during cDNA microarray analysis, we used a 2-step method of semi-quantitative RT-PCR starting with tissues from 32 cases of laryngeal cancer and matched normal adjacent tissues. .. For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720.

Modification:

Article Title: Selection of Aptamers Specific for Adipose Tissue
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM), calf serum (CS), FBS, Oil Red O, phosphate-buffered saline (PBS), paraformaldehyde, bovine serum albumin (BSA), 3-isobutyl-1-methyxanthine (IBMX), insulin and dexamethasone (DEX) were purchased from Sigma (St. Louis, MO). .. Taq-polymerase, dNTPs and other reagents were purchased from Takara.

High Performance Liquid Chromatography:

Article Title: Selection of Aptamers Specific for Adipose Tissue
Article Snippet: The sequences were then deprotected in AMA (ammonium hydroxide/40% aqueous methylamine 1∶1) and purified by reversed-phase HPLC (ProStar, Varian, Walnut Creek, CA, USA). .. Taq-polymerase, dNTPs and other reagents were purchased from Takara.

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. A high-performance liquid chromatography system (Hitachi, Tokyo, Japan) was used for squalene analysis.

Sequencing:

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: Oligonucleotide primers (Integrated DNA Technology, Coralville, USA) ( ) were designed using a PrimerQuest Tool ( https://www.idtdna.com/Primerquest/Home/Index ) based on the genomic sequence of S . pseudintermedius strain 06–3228 (20). .. PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes.

Positive Control:

Article Title: Clinical verification of plasma messenger RNA as novel noninvasive biomarker identified through bioinformatics analysis for lung cancer
Article Snippet: The target sequences were amplified by PCR in 50μl of 1×Taq buffer containing 0.3μM of each primer, 1.5 mM magnesium chloride, 200μM dNTP mixture, 2.5 units of Taq polymerase and 1μl (10μg) of each cDNA by Ex Taq PCR kit (TAKARA cat#RR001A). .. Although a range of annealing temperatures (from 5°C below the Tm to 5°C above the Tm) had been tried, the PCR products of other 9 mRNAs (TOP2A, GINS2, TK1, CDCA5, AGER, FHL1 CLDN18, ADH1B, and GPIHBP1) were rare compared with positive control.

Cell Culture:

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. Engineered strains for squalene overproduction were cultured at 37 °C in LB medium (10 g/L NaCl, 10 g/L peptone, 5 g/L yeast extract) supplemented with 10 g/L glucose and 1.0 g/L MgSO4 ·7H2 O.

DNA Sequencing:

Article Title: Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
Article Snippet: Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan). .. Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes
Article Snippet: .. One microgram of total RNA was converted to cDNA by superscript reverse transcriptase and then amplified by a Taq polymerase using reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. The relative expression of iNOS, COX-2, filaggrin, and SPT were analyzed using PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control.

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: .. The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.). .. PCR conditions were as follows: 95°C for 2 min, followed by 40 cycles of 94°C for 20 sec, 60°C for 20 sec and 72°C for 30 sec, and a final elongation at 72°C for 10 min. Primers were as follows: TLR4 forward, 5′-AAGCCGAAAGGTGATTGTTG-3′ and reverse, 5′-CTGAGCAGGGTCTTCTCCAC-3′; β-actin (reference gene) forward, 5′-AGCGAGCATCCCCCAAAGTT-3′ and reverse, 5′-GGGCACGAAGGCTCATCATT-3′.

Article Title: Clinical verification of plasma messenger RNA as novel noninvasive biomarker identified through bioinformatics analysis for lung cancer
Article Snippet: Paragraph title: Conventional RT-PCR analysis ... The target sequences were amplified by PCR in 50μl of 1×Taq buffer containing 0.3μM of each primer, 1.5 mM magnesium chloride, 200μM dNTP mixture, 2.5 units of Taq polymerase and 1μl (10μg) of each cDNA by Ex Taq PCR kit (TAKARA cat#RR001A).

Article Title: 2,5-Dihydroxyacetophenone Induces Apoptosis of Multiple Myeloma Cells by Regulating the MAPK Activation Pathway
Article Snippet: .. One microgram of total RNA was changed to cDNA via reverse transcriptase, then amplified with a Taq polymerase via the use of an RT-PCR kit (Takara Bio Inc., Tokyo, Japan). .. The relative expressions of Bcl-xl , Bcl-2 , Cyclin D1 , Survivin , MMP-9 , COX-2 , Bax , and p21 were analyzed using a TaKaRa PCR Thermal Cycler (Code TP350, Takara Bio Inc., Tokyo, Japan) with glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) as an internal control.

Article Title: Anti-myeloma Effects of Icariin Are Mediated Through the Attenuation of JAK/STAT3-Dependent Signaling Cascade
Article Snippet: .. Then RNA was Reverse transcribed into cDNA using superscript reverse transcriptase and Taq polymerase by reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). ..

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: Paragraph title: Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) ... For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720.

DNA Extraction:

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: DNA was extracted using a MO BIO DNA Isolation Kit (QIAGEN Inc. Cat No.12224-50) according to the manufacturer’s instructions. .. PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes.

Article Title: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Article Snippet: Genomic DNA was extracted using a Bacterial genomic DNA Extraction Kit (TIANGEN, Beijing, China). .. Taq polymerase and T4 DNA ligase were purchased from Takara (Dalian, China).

Mutagenesis:

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: The native LukS-I and LukF-I open reading frames (ORF) (933 and 981 bp, respectively) without the regions encoding the predicted N-terminal signal peptide were amplified from S . pseudintermedius 06–3228 genomic DNA and the ORF of mutant LukS-I and LukF-I were amplified from pMA-attenuated LukS-I -M and pMA-attenuated LukF-I -M plasmids (Life Technologies Corp., Carlsbad, CA), respectively ( ) . .. PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes.

Isolation:

Article Title: Genetic Variations in ABCG2 Gene Predict Breast Carcinoma Susceptibility and Clinical Outcomes after Treatment with Anthracycline-Based Chemotherapy
Article Snippet: Genotyping Analysis Genomic DNA was isolated from a leukocyte cell pellet of each blood sample according to the TIANGEN manufacturer's instructions. .. PCR amplification was performed as follows: 100 ng of genomic DNA, 300 nM of each primer, 200 nM dNTPs, and 0.5 U Taq polymerase in PCR buffer (TaKaRa Biotechnology (Dalian) Co. Ltd., Dalian, China).

Size-exclusion Chromatography:

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.). .. PCR conditions were as follows: 95°C for 2 min, followed by 40 cycles of 94°C for 20 sec, 60°C for 20 sec and 72°C for 30 sec, and a final elongation at 72°C for 10 min. Primers were as follows: TLR4 forward, 5′-AAGCCGAAAGGTGATTGTTG-3′ and reverse, 5′-CTGAGCAGGGTCTTCTCCAC-3′; β-actin (reference gene) forward, 5′-AGCGAGCATCCCCCAAAGTT-3′ and reverse, 5′-GGGCACGAAGGCTCATCATT-3′.

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: .. For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720. .. The PCR reactions were performed in triplicate.

Purification:

Article Title: Selection of Aptamers Specific for Adipose Tissue
Article Snippet: The sequences were then deprotected in AMA (ammonium hydroxide/40% aqueous methylamine 1∶1) and purified by reversed-phase HPLC (ProStar, Varian, Walnut Creek, CA, USA). .. Taq-polymerase, dNTPs and other reagents were purchased from Takara.

Article Title: Anti-myeloma Effects of Icariin Are Mediated Through the Attenuation of JAK/STAT3-Dependent Signaling Cascade
Article Snippet: Cells were suspended in Trizol, and RNA purified using chloroform and isopropanol. .. Then RNA was Reverse transcribed into cDNA using superscript reverse transcriptase and Taq polymerase by reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan).

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. Gel extraction kit, PCR purification kit and plasmid purification kit were purchased from QIAGEN (Hilden, Germany).

Article Title: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Article Snippet: DNA fragments from polymerase chain reactions (PCRs) and restriction enzyme digestions were purified using the AxyPrep Gel Extraction Kit (Axygen, Union City, CA, USA). .. Taq polymerase and T4 DNA ligase were purchased from Takara (Dalian, China).

Polymerase Chain Reaction:

Article Title: A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis
Article Snippet: .. To test the efficiency of Taq Polymerase to be employed in PCR assays, comparative tests were made with several Taq polymerases, such as Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase, and TaKaRa Taq ™. .. The Phire™ HotStart DNA polymerase, coupled with a preoptimized primer mix for different multiplex reactions, gave the best results both in terms of reproducibility and robustness.

Article Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Article Snippet: .. PCR was performed using taq polymerase (rTaq, Takara, Cat No. R004) and the following cycling conditions were performed: initial denaturation at 95°C for 90 seconds, 30 cycles of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds and extension at 72°C for 1 minute followed by a final extension at 72°C for 5 minutes. .. All ORFs were amplified without a histidine tag because pETBlue-2 allowed T7lac promoter-based expression of target genes with C-terminal histidine tagged sequences.

Article Title: Fermented dried Citrus unshiu peel extracts exert anti-inflammatory activities in LPS-induced RAW264.7 macrophages and improve skin moisturizing efficacy in immortalized human HaCaT keratinocytes
Article Snippet: One microgram of total RNA was converted to cDNA by superscript reverse transcriptase and then amplified by a Taq polymerase using reverse transcription polymerase chain reaction (RT-PCR) (TAKARA, Tokyo, Japan). .. The relative expression of iNOS, COX-2, filaggrin, and SPT were analyzed using PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an internal control.

Article Title: Expression of TLR4 gene is downregulated in acquired immune deficiency syndrome-associated Kaposi's sarcoma
Article Snippet: .. The PCR reaction system contained cDNA (1 µl), 10X buffer (2 µl), MgCl2 (1 µl), SYBR PrimeScript RT-PCR kit assay mix (1 µl), double distilled H2 O (14.8 µl) and Taq polymerase (0.2 µl) (SYBR PrimeScript RT-PCR kit; Takara Biotechnology Co., Ltd.). .. PCR conditions were as follows: 95°C for 2 min, followed by 40 cycles of 94°C for 20 sec, 60°C for 20 sec and 72°C for 30 sec, and a final elongation at 72°C for 10 min. Primers were as follows: TLR4 forward, 5′-AAGCCGAAAGGTGATTGTTG-3′ and reverse, 5′-CTGAGCAGGGTCTTCTCCAC-3′; β-actin (reference gene) forward, 5′-AGCGAGCATCCCCCAAAGTT-3′ and reverse, 5′-GGGCACGAAGGCTCATCATT-3′.

Article Title: Genetic Variations in ABCG2 Gene Predict Breast Carcinoma Susceptibility and Clinical Outcomes after Treatment with Anthracycline-Based Chemotherapy
Article Snippet: .. PCR amplification was performed as follows: 100 ng of genomic DNA, 300 nM of each primer, 200 nM dNTPs, and 0.5 U Taq polymerase in PCR buffer (TaKaRa Biotechnology (Dalian) Co. Ltd., Dalian, China). .. The reaction for amplification was carried out in the following conditions: an initial melting step of 5 min at 94°C, followed by 35 cycles of 30 s at 94°C, 30 s at 58°C, and 1 min at 72°C and a final elongation of 5 min at 72°C.

Article Title: Clinical verification of plasma messenger RNA as novel noninvasive biomarker identified through bioinformatics analysis for lung cancer
Article Snippet: .. The target sequences were amplified by PCR in 50μl of 1×Taq buffer containing 0.3μM of each primer, 1.5 mM magnesium chloride, 200μM dNTP mixture, 2.5 units of Taq polymerase and 1μl (10μg) of each cDNA by Ex Taq PCR kit (TAKARA cat#RR001A). ..

Article Title: 2,5-Dihydroxyacetophenone Induces Apoptosis of Multiple Myeloma Cells by Regulating the MAPK Activation Pathway
Article Snippet: One microgram of total RNA was changed to cDNA via reverse transcriptase, then amplified with a Taq polymerase via the use of an RT-PCR kit (Takara Bio Inc., Tokyo, Japan). .. The relative expressions of Bcl-xl , Bcl-2 , Cyclin D1 , Survivin , MMP-9 , COX-2 , Bax , and p21 were analyzed using a TaKaRa PCR Thermal Cycler (Code TP350, Takara Bio Inc., Tokyo, Japan) with glyceraldehyde-3-phosphate dehydrogenase (GAPDH ) as an internal control.

Article Title: Selection of Aptamers Specific for Adipose Tissue
Article Snippet: Taq-polymerase, dNTPs and other reagents were purchased from Takara. .. PCR was performed on a Biorad Thermocycler.

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. Gel extraction kit, PCR purification kit and plasmid purification kit were purchased from QIAGEN (Hilden, Germany).

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: .. For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720. .. The PCR reactions were performed in triplicate.

Staining:

Article Title: 2,5-Dihydroxyacetophenone Induces Apoptosis of Multiple Myeloma Cells by Regulating the MAPK Activation Pathway
Article Snippet: One microgram of total RNA was changed to cDNA via reverse transcriptase, then amplified with a Taq polymerase via the use of an RT-PCR kit (Takara Bio Inc., Tokyo, Japan). .. PCR products were run on 1% agarose gel, after which they were stained with loading star (Dynebio, Gyeonggi, Korea).

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720. .. The PCR-amplified gene products were visualized in a 2% (w/v) agarose gel stained with ethidium bromide.

Chloramphenicol Acetyltransferase Assay:

Article Title: Clinical verification of plasma messenger RNA as novel noninvasive biomarker identified through bioinformatics analysis for lung cancer
Article Snippet: .. The target sequences were amplified by PCR in 50μl of 1×Taq buffer containing 0.3μM of each primer, 1.5 mM magnesium chloride, 200μM dNTP mixture, 2.5 units of Taq polymerase and 1μl (10μg) of each cDNA by Ex Taq PCR kit (TAKARA catRR001A). ..

Plasmid Preparation:

Article Title: Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
Article Snippet: P . pastoris strain KM71 was grown in YPD broth (2% tryptone, 1% yeast extract, 2% dextrose) at 28 °C, or BMMY broth (1% yeast extract, 1.34% YNB, 4 × 10−5 % biotin, 2% dextrose, 100 mM potassium buffer, 0.5% methanol) at 28 °C, or MD broth plates (2% dextrose, 4 × 10−5 % biotin, 1.34% YNB and 2% agar) at 28 °C for screening yeast recombinants, or tributyrin agar plate (2% tryptone, 1% yeast extract, 1.34% YNB, 100 mM potassium buffer, 4 × 10−5 % biotin, 1% glycerol at 16 °C, 1% tributyrin and 2% agar) at 28 °C. pMD19-T cloning vector (Takara, Otsu, Japan), pET-28a (Novagen, Darmstadt, Germany) and the plasmid pPIC9K (Invitrogen, Carlsbad, USA) were applied for constructing the expression plasmids. .. Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan).

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. Gel extraction kit, PCR purification kit and plasmid purification kit were purchased from QIAGEN (Hilden, Germany).

Article Title: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Article Snippet: Plasmid DNA was extracted using an AxyPrep Plasmid Miniprep kit (Axygen, Union City, CA, USA). .. Taq polymerase and T4 DNA ligase were purchased from Takara (Dalian, China).

Multiplex Assay:

Article Title: A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis
Article Snippet: Optimization of the UP-M-PCR The concentrations of primers strongly influence the efficiency and disparity of PCR reaction, which is very important for the PCR reaction, especially in multiplex PCR. .. To test the efficiency of Taq Polymerase to be employed in PCR assays, comparative tests were made with several Taq polymerases, such as Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase, and TaKaRa Taq ™.

Positron Emission Tomography:

Article Title: Heterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
Article Snippet: P . pastoris strain KM71 was grown in YPD broth (2% tryptone, 1% yeast extract, 2% dextrose) at 28 °C, or BMMY broth (1% yeast extract, 1.34% YNB, 4 × 10−5 % biotin, 2% dextrose, 100 mM potassium buffer, 0.5% methanol) at 28 °C, or MD broth plates (2% dextrose, 4 × 10−5 % biotin, 1.34% YNB and 2% agar) at 28 °C for screening yeast recombinants, or tributyrin agar plate (2% tryptone, 1% yeast extract, 1.34% YNB, 100 mM potassium buffer, 4 × 10−5 % biotin, 1% glycerol at 16 °C, 1% tributyrin and 2% agar) at 28 °C. pMD19-T cloning vector (Takara, Otsu, Japan), pET-28a (Novagen, Darmstadt, Germany) and the plasmid pPIC9K (Invitrogen, Carlsbad, USA) were applied for constructing the expression plasmids. .. Taq polymerase, Restriction enzyme and T4 ligase were obtained from Takara (Otsu, Japan).

Agarose Gel Electrophoresis:

Article Title: 2,5-Dihydroxyacetophenone Induces Apoptosis of Multiple Myeloma Cells by Regulating the MAPK Activation Pathway
Article Snippet: One microgram of total RNA was changed to cDNA via reverse transcriptase, then amplified with a Taq polymerase via the use of an RT-PCR kit (Takara Bio Inc., Tokyo, Japan). .. PCR products were run on 1% agarose gel, after which they were stained with loading star (Dynebio, Gyeonggi, Korea).

Article Title: Detection of differentially expressed genes and association with clinicopathological features in laryngeal squamous cell carcinoma
Article Snippet: For each reaction, a 50- μ l PCR mixture containing 200 μ M dNTPs, 1.25 units Taq polymerase in 10X Taq polymerase buffer (Takara Bio, Inc., Shiga, Japan), and corresponding concentrations of primers ( ) was set to an initial denaturing at 95°C for 5 min and then appropriate PCR cycles for different genes of 94°C for 1 min, annealing temperature ( ) for 1 min, 72°C for 30 sec and a final extension at 72°C for 10 min in a programmable 2720. .. The PCR-amplified gene products were visualized in a 2% (w/v) agarose gel stained with ethidium bromide.

Concentration Assay:

Article Title: A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis
Article Snippet: The final optimized concentration of the compound specific primers were 10 nmol L−1 for 35s-195-F/R and Nos-F/R (about 1/50 of UP), 16 nmol L−1 for nptII-508-F/R and Pa-363-F/R(about 1/30 of UP), 50 nmol L−1 for hpt-839-F/R, Ivr-262-F/R and Lec-110-F/R(about 1/10 of UP), and 25 nmol L−1 for all other primers (including gus-565-F/R, aadA-406-F/R, pat-262-F/R, bar-177-F/R, sps-110-F/R, sad1-91-F/R, uidA-82-F/R and FatA-76-F/R). .. To test the efficiency of Taq Polymerase to be employed in PCR assays, comparative tests were made with several Taq polymerases, such as Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase, and TaKaRa Taq ™.

Gel Extraction:

Article Title: Improving squalene production by enhancing the NADPH/NADP+ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli
Article Snippet: Restriction enzymes, Taq polymerase and T4 ligase were purchased from Takara (Dalian City, China). .. Gel extraction kit, PCR purification kit and plasmid purification kit were purchased from QIAGEN (Hilden, Germany).

Article Title: Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
Article Snippet: DNA fragments from polymerase chain reactions (PCRs) and restriction enzyme digestions were purified using the AxyPrep Gel Extraction Kit (Axygen, Union City, CA, USA). .. Taq polymerase and T4 DNA ligase were purchased from Takara (Dalian, China).

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    TaKaRa la pcr kit
    La Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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