Structured Review

TaKaRa taq dna polymerase
Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq dna polymerase/product/TaKaRa
Average 99 stars, based on 1599 article reviews
Price from $9.99 to $1999.99
taq dna polymerase - by Bioz Stars, 2020-04
99/100 stars

Images

Related Articles

Amplification:

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: Polymorphism identification and genotyping with polymerase chain reaction-restriction fragment length polymorphism Genetic variation of the pCISH were analyzed by sequencing PCR products amplified by primer pairs CV-1, CV-2, CV-3, CV-4, and CV-5 , and all these primers were partially overlapped. .. Two PCR-restriction fragment length polymorphism (RFLP) assays were designed to genotype the selected single nucleotide polymorphisms (SNPs) using primer pairs CV-1, CV-41 ( ) and restriction enzymes Taq I (TaKaRa, Japan), Hae III (TaKaRa, Japan), respectively.

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: .. For the PCR-RFLP profile, 8.5 μL of PCR products amplified by primer pairs CV-1 or CV-41 were digested with 5 U of Taq I or Hae III (TaKaRa, Japan) for 5 h at 65°C or 37°C, and then separated on a 1.5% agarose gel. ..

Reporter Assay:

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) and luciferase reporter assay ... RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan).

Electrophoresis:

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Sequences of qPCR products were confirmed using an ABI 7700 model sequence detector with SDS v.-1.6.3 software (Applied Biosystems, Foster City, California, USA) at the Cosmo sequence facility service (Seoul, Korea) after electrophoresis using a 2% agarose gel; gene sequences were aligned using Clone Manager suite 7 (Sci-Ed Software, Cary, North Carolina, USA). .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Luciferase:

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: Paragraph title: Reverse transcription-polymerase chain reaction (RT-PCR) and luciferase reporter assay ... RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan).

Activity Assay:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Modification:

Article Title: Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Article Snippet: An aliquot (15 µl) of the qPCR product was digested with Taq I (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and DNA fragments were analyzed using 2.5% agarose gels. .. Modified acid-fast staining was performed on stool samples that were positive for C. hominis by using qPCR-based RFLP.

Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA
Article Snippet: Materials The following commercial available thermostable DNA polymerases were purchased: Taq (Takara Bio), Tth (Toyobo), Vent(exo-) (New England Biolabs) and KOD Dash (Toyobo). .. All modified nucleoside triphosphates used for PCR assays are listed in .

Western Blot:

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes by using a DNA Extractor WB Kit (Wako Pure Chemical Industries, Osaka, Japan). .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ].

Transfection:

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: After transfection for 48 h, the cells were exposed to 1 μg/mL LPS for 6 h. Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and 1 μg of RNA was reversely transcribed into cDNA, which was then subjected to 20–30 cycles of PCR (Applied Biosystems, Foster City, CA, USA). .. RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan).

Sequencing:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: Sequences of qPCR products were confirmed using the ABI 7700 Sequence Detector and SDS software (version 1.6.3; Applied Biosystems, Foster City, CA, USA) at the Cosmo Sequence Facility Service (Seoul, Korea). .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel.

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Sequences of qPCR products were confirmed using an ABI 7700 model sequence detector with SDS v.-1.6.3 software (Applied Biosystems, Foster City, California, USA) at the Cosmo sequence facility service (Seoul, Korea) after electrophoresis using a 2% agarose gel; gene sequences were aligned using Clone Manager suite 7 (Sci-Ed Software, Cary, North Carolina, USA). .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Inverse PCR:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: .. Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: .. Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Generated:

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: A 740-base-pair (bp) fragment was generated by PCR with primers located on intron 8 and exon 9. .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ].

CpG Methylation Assay:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence.

Polymerase Chain Reaction:

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: .. Two PCR-restriction fragment length polymorphism (RFLP) assays were designed to genotype the selected single nucleotide polymorphisms (SNPs) using primer pairs CV-1, CV-41 ( ) and restriction enzymes Taq I (TaKaRa, Japan), Hae III (TaKaRa, Japan), respectively. .. For the PCR-RFLP profile, 8.5 μL of PCR products amplified by primer pairs CV-1 or CV-41 were digested with 5 U of Taq I or Hae III (TaKaRa, Japan) for 5 h at 65°C or 37°C, and then separated on a 1.5% agarose gel.

Article Title: HapMap-based study of CIP2A gene polymorphisms and HCC susceptibility
Article Snippet: .. The PCR products were then digested by restriction enzyme Taq I (Takara Biotechnology Co., Ltd.) at 65°C overnight. .. The digested products were subsequently separated using 2% agarose gel electrophoresis, stained with ethidium bromide and visualized under ultraviolet light.

Article Title: Systematic characterization of 2?-deoxynucleoside- 5?-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA
Article Snippet: Materials The following commercial available thermostable DNA polymerases were purchased: Taq (Takara Bio), Tth (Toyobo), Vent(exo-) (New England Biolabs) and KOD Dash (Toyobo). .. All modified nucleoside triphosphates used for PCR assays are listed in .

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: The sequences of the PCR primers and the sizes of PCR products are shown in . .. RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan).

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ]. ..

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: .. For the PCR-RFLP profile, 8.5 μL of PCR products amplified by primer pairs CV-1 or CV-41 were digested with 5 U of Taq I or Hae III (TaKaRa, Japan) for 5 h at 65°C or 37°C, and then separated on a 1.5% agarose gel. ..

Cellular Antioxidant Activity Assay:

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: The primer sequences were 5′ cag agc atg gac agg gag caa 3′ (forward) and 5′ gca act cct cat ggc tga ggt ctc 3′ (reverse). .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ].

DNA Extraction:

Article Title: HapMap-based study of CIP2A gene polymorphisms and HCC susceptibility
Article Snippet: The DNA isolation kit (Dingguo Biotechnology Co., Ltd., Beijing, China) was used to extract the genomic DNA from 1 ml peripheral blood sample. .. The PCR products were then digested by restriction enzyme Taq I (Takara Biotechnology Co., Ltd.) at 65°C overnight.

Isolation:

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: After transfection for 48 h, the cells were exposed to 1 μg/mL LPS for 6 h. Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and 1 μg of RNA was reversely transcribed into cDNA, which was then subjected to 20–30 cycles of PCR (Applied Biosystems, Foster City, CA, USA). .. RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan).

Size-exclusion Chromatography:

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Briefly, reaction mixtures included 0.1×LightCycler® FastStart HybProbe master mix (Roche, Mannheim, Germany), each C. parvum primer set at 0.5 μM (Bioneer, Daejeon, Korea), and the probe set at 0.1 μM (TIB MOLBIO, Berlin, Germany). qPCR was performed using a LightCycler® 480 device (Roche), and each qPCR mixture was denatured at 95°C for 10 min, followed by 45 cycles of denaturation at 95°C for 10 sec, annealing at 60°C for 30 sec, and extension at 72°C for 3 sec, with a final cooling step at 40°C for 30 sec. DNase/RNase-free water was used in place of template DNA as a negative control. .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: HapMap-based study of CIP2A gene polymorphisms and HCC susceptibility
Article Snippet: A thermocycler was used to perform PCR on rs2278911 , as follows: 95°C for 5 min, 35 cycles of 95°C for 30 sec, 54°C for 40 sec, 72°C for 45 sec and extension at 72°C for 10 min. .. The PCR products were then digested by restriction enzyme Taq I (Takara Biotechnology Co., Ltd.) at 65°C overnight.

Purification:

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: The PCR products from Minzhu, Landrace, and Largewhite pig breeds were purified, sequenced and then compared with each other using Clustalw 2.0. .. Two PCR-restriction fragment length polymorphism (RFLP) assays were designed to genotype the selected single nucleotide polymorphisms (SNPs) using primer pairs CV-1, CV-41 ( ) and restriction enzymes Taq I (TaKaRa, Japan), Hae III (TaKaRa, Japan), respectively.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Lobolide, a diterpene, blockades the NF-κB pathway and p38 and ERK MAPK activity in macrophages in vitro
Article Snippet: .. RT-PCR was performed in two steps following the instructions included with the RTase M-MLV (RNase H– ) and Taq enzymes (Takara Shuzo, Kyoto, Japan). .. For the luciferase reporter assay, the same transfection protocol was used as that for RT-PCR, except that the cells were seeded in 96-well plates.

Nested PCR:

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ]. .. For Cyclospora species identification, we performed nested PCR as described by Orlandi and Lampel [ ] with qPCR positive vegetable samples and the 18S ribosomal DNA gene as the target.

Activated Clotting Time Assay:

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: The primer sequences were 5′ cag agc atg gac agg gag caa 3′ (forward) and 5′ gca act cct cat ggc tga ggt ctc 3′ (reverse). .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ].

Plasmid Preparation:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: The plasmid DNA standard for qPCR was prepared as described previously using Rad 16 as the target gene ( ). .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel.

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: The plasmid DNA standard for real-time PCR was prepared as described previously using the Rad 16 gene of Cryptosporidium and ITS2 gene of Cyclospora as targets [ ]. .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Software:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: The gene sequences were aligned using Clone ManagerSuite 7 (Sci-Ed Software, Cary, NC, USA). .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel.

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Sequences of qPCR products were confirmed using an ABI 7700 model sequence detector with SDS v.-1.6.3 software (Applied Biosystems, Foster City, California, USA) at the Cosmo sequence facility service (Seoul, Korea) after electrophoresis using a 2% agarose gel; gene sequences were aligned using Clone Manager suite 7 (Sci-Ed Software, Cary, North Carolina, USA). .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Article Snippet: CP2 sequences of C. parvum (AY471868) and C. hominis (XM_661199) were aligned using Clone Manager Suite 7 (Sci-Ed Software, Cary, North Carolina, USA), and restriction enzyme cleavage sites were identified using NEBcutter V2.0 ( http://tools.neb.com/NEBcutter2/ ). .. An aliquot (15 µl) of the qPCR product was digested with Taq I (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and DNA fragments were analyzed using 2.5% agarose gels.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Inverse PCR Inverse PCR were performed using restriction enzyme Taq I (TaKaRa, Shiga, Japan) to isolate the unknown sequences adjacent to the duplicated region of the OTC gene in the study patient. .. ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme.

Real-time Polymerase Chain Reaction:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel. ..

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ]. .. For Cyclospora species identification, we performed nested PCR as described by Orlandi and Lampel [ ] with qPCR positive vegetable samples and the 18S ribosomal DNA gene as the target.

Article Title: Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Article Snippet: .. An aliquot (15 µl) of the qPCR product was digested with Taq I (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and DNA fragments were analyzed using 2.5% agarose gels. ..

Negative Control:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: DNase/RNase-free water was used instead of the template DNA as a negative control. .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel.

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Briefly, reaction mixtures included 0.1×LightCycler® FastStart HybProbe master mix (Roche, Mannheim, Germany), each C. parvum primer set at 0.5 μM (Bioneer, Daejeon, Korea), and the probe set at 0.1 μM (TIB MOLBIO, Berlin, Germany). qPCR was performed using a LightCycler® 480 device (Roche), and each qPCR mixture was denatured at 95°C for 10 min, followed by 45 cycles of denaturation at 95°C for 10 sec, annealing at 60°C for 30 sec, and extension at 72°C for 3 sec, with a final cooling step at 40°C for 30 sec. DNase/RNase-free water was used in place of template DNA as a negative control. .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Article Snippet: DNase/RNase-free water was used in place of template DNA as a negative control. .. An aliquot (15 µl) of the qPCR product was digested with Taq I (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and DNA fragments were analyzed using 2.5% agarose gels.

Agarose Gel Electrophoresis:

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: .. To differentiate Cryptosporidium sp. by using the restriction enzyme fragmentation method, the qPCR products were digested with Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and the DNA fragments were analyzed on 2% agarose gel. ..

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: Two PCR-restriction fragment length polymorphism (RFLP) assays were designed to genotype the selected single nucleotide polymorphisms (SNPs) using primer pairs CV-1, CV-41 ( ) and restriction enzymes Taq I (TaKaRa, Japan), Hae III (TaKaRa, Japan), respectively. .. For the PCR-RFLP profile, 8.5 μL of PCR products amplified by primer pairs CV-1 or CV-41 were digested with 5 U of Taq I or Hae III (TaKaRa, Japan) for 5 h at 65°C or 37°C, and then separated on a 1.5% agarose gel.

Article Title: Simultaneous Molecular Detection of Cryptosporidium and Cyclospora from Raw Vegetables in Korea
Article Snippet: Sequences of qPCR products were confirmed using an ABI 7700 model sequence detector with SDS v.-1.6.3 software (Applied Biosystems, Foster City, California, USA) at the Cosmo sequence facility service (Seoul, Korea) after electrophoresis using a 2% agarose gel; gene sequences were aligned using Clone Manager suite 7 (Sci-Ed Software, Cary, North Carolina, USA). .. To differentiate Cryptosporidium species, the qPCR products were digested with the Taq I restriction enzyme (Takara Bio Inc., Shiga, Japan) at 65°C for 2 hr, and analyzed using 2% agarose gels [ ].

Article Title: HapMap-based study of CIP2A gene polymorphisms and HCC susceptibility
Article Snippet: The PCR products were then digested by restriction enzyme Taq I (Takara Biotechnology Co., Ltd.) at 65°C overnight. .. The digested products were subsequently separated using 2% agarose gel electrophoresis, stained with ethidium bromide and visualized under ultraviolet light.

Article Title: Detection of Cryptosporidium parvum in Environmental Soil and Vegetables
Article Snippet: .. Agarose gel electrophoresis revealed a 242-bp product from all Cryptosporidium -positive samples , and Taq I restriction enzyme digest revealed that all positive samples had fragmented into 117- and 125-bp bands ( ). ..

Article Title: Association between bone mineral density and lifestyle factors or vitamin D receptor gene polymorphism in adult male workers: a cross-sectional study
Article Snippet: .. PCR was performed for 35 cycles using Taq polymerase (Perkin Elmer Co., Ltd., NJ, USA) under the following conditions: denaturation at 94°C for 1 min, annealing at 57°C for 1 min, and extension at 72°C for 1 min. Then 10 μl of the PCR products were subjected to digestion with Taq I (Takara Shuzo Co., Ltd., Kyoto, Japan) at 65°C for 3 h and were separated on 3% Nusieve agarose gel (FMC Bioproducts, Rockland, ME, USA) [ ]. ..

Article Title: Characterization of porcine cytokine inducible SH2-containing protein gene and its association with piglet diarrhea traits
Article Snippet: .. For the PCR-RFLP profile, 8.5 μL of PCR products amplified by primer pairs CV-1 or CV-41 were digested with 5 U of Taq I or Hae III (TaKaRa, Japan) for 5 h at 65°C or 37°C, and then separated on a 1.5% agarose gel. ..

Staining:

Article Title: HapMap-based study of CIP2A gene polymorphisms and HCC susceptibility
Article Snippet: The PCR products were then digested by restriction enzyme Taq I (Takara Biotechnology Co., Ltd.) at 65°C overnight. .. The digested products were subsequently separated using 2% agarose gel electrophoresis, stained with ethidium bromide and visualized under ultraviolet light.

Article Title: Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
Article Snippet: An aliquot (15 µl) of the qPCR product was digested with Taq I (Takara Bio Inc., Shiga, Japan) at 65℃ for 2 hr, and DNA fragments were analyzed using 2.5% agarose gels. .. Modified acid-fast staining was performed on stool samples that were positive for C. hominis by using qPCR-based RFLP.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa taq dna polymerase
    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic <t>DNA.</t> PCR was performed on the isolated genomic DNA using <t>taq</t> DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/TaKaRa
    Average 99 stars, based on 1599 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    TaKaRa pcr amplification
    Co-transformation of <t>Phlebia</t> sp. strain MG-60 with p PbGPD -MG mnp 2 and p PbGPD-HPT . A , Detection of the MG mnp2 gene in 10 of 14 regenerated protoplasts co-transformed with p PbGPD-HPT and p PbGPD -MG mnp 2 by <t>PCR</t> amplification using primers PbGPD-prom -F1 and g MG mnp2-Asc- R1. M indicates a 1 kb ladder size marker, Wt indicates the wild type, HPT indicates the hygromycin resistant transformant, and M1–M14 indicate MG mnp2 regenerated transformants. B , MnP activity in Kirk’s high-nitrogen culture medium under aerobic condition for 3 d (white column) and 6 d (black column). One unit of MnP activity was defined as 1 μmol reaction product formed per minute. C , MnP activity of various strains in the extracts of Quercus wood powder medium under aerobic condition for 20 d. The tests, using three flasks per strain, were carried out independently. Data are means ± SE (n = 3) and values without a common superscript letter are significantly different at p
    Pcr Amplification, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr amplification/product/TaKaRa
    Average 99 stars, based on 611 article reviews
    Price from $9.99 to $1999.99
    pcr amplification - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Journal: Neuroscience letters

    Article Title: Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain

    doi: 10.1016/j.neulet.2010.12.060

    Figure Lengend Snippet: (A) Genotype in each generation of mutant mice confirmed by PCR analysis on isolated genomic DNA. PCR was performed on the isolated genomic DNA using taq DNA polymerase and primer sets (#1 and #2 are for wild-type and GluR2 delta7 KI, #3 and #4 are for

    Article Snippet: PCR was performed on the isolated genomic DNA using taq DNA polymerase (TaKaRa Ex Taq™, Takara, Otsu, Japan) and primer sets (primer #21–23 for GluR2 delta7: #21, 5′-ACA GAG GAA GGT AGT GGA AGG GAG-3′; #22, 5′-CTT GGT TTG GTT GTT GGT CAT AGC-3′; #23, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′; primer #31–33 for GluR3 delta7: #31, 5′-CCA ATA CTC CAC AGG GGC AAT TTA TC-3′; #32, 5′-CCG TTG ACT GTT TTG AAT CTC ACA CC-3′; #33, 5′-CTA GTG AAC CTC TTC GAG GGA C-3′).

    Techniques: Mutagenesis, Mouse Assay, Polymerase Chain Reaction, Isolation

    Co-transformation of Phlebia sp. strain MG-60 with p PbGPD -MG mnp 2 and p PbGPD-HPT . A , Detection of the MG mnp2 gene in 10 of 14 regenerated protoplasts co-transformed with p PbGPD-HPT and p PbGPD -MG mnp 2 by PCR amplification using primers PbGPD-prom -F1 and g MG mnp2-Asc- R1. M indicates a 1 kb ladder size marker, Wt indicates the wild type, HPT indicates the hygromycin resistant transformant, and M1–M14 indicate MG mnp2 regenerated transformants. B , MnP activity in Kirk’s high-nitrogen culture medium under aerobic condition for 3 d (white column) and 6 d (black column). One unit of MnP activity was defined as 1 μmol reaction product formed per minute. C , MnP activity of various strains in the extracts of Quercus wood powder medium under aerobic condition for 20 d. The tests, using three flasks per strain, were carried out independently. Data are means ± SE (n = 3) and values without a common superscript letter are significantly different at p

    Journal: SpringerPlus

    Article Title: Expression of a manganese peroxidase isozyme 2 transgene in the ethanologenic white rot fungus Phlebia sp. strain MG-60

    doi: 10.1186/2193-1801-3-699

    Figure Lengend Snippet: Co-transformation of Phlebia sp. strain MG-60 with p PbGPD -MG mnp 2 and p PbGPD-HPT . A , Detection of the MG mnp2 gene in 10 of 14 regenerated protoplasts co-transformed with p PbGPD-HPT and p PbGPD -MG mnp 2 by PCR amplification using primers PbGPD-prom -F1 and g MG mnp2-Asc- R1. M indicates a 1 kb ladder size marker, Wt indicates the wild type, HPT indicates the hygromycin resistant transformant, and M1–M14 indicate MG mnp2 regenerated transformants. B , MnP activity in Kirk’s high-nitrogen culture medium under aerobic condition for 3 d (white column) and 6 d (black column). One unit of MnP activity was defined as 1 μmol reaction product formed per minute. C , MnP activity of various strains in the extracts of Quercus wood powder medium under aerobic condition for 20 d. The tests, using three flasks per strain, were carried out independently. Data are means ± SE (n = 3) and values without a common superscript letter are significantly different at p

    Article Snippet: The PbGPD gene was obtained from Phlebia brevispora HHB-7030 genomic DNA (protein ID: 29450) by PCR amplification with the primers PbGPD -F1 and PbGPD -R1, and the amplified fragment was ligated into the T-Vector pMD20 (TAKARA BIO INC, Shiga, Japan) (steps 1 and 2).

    Techniques: Transformation Assay, Polymerase Chain Reaction, Amplification, Marker, Activity Assay

    Expression and localization of S100A14 and S100A16 in human breast cancer cell lines. A , Relative mRNA expression levels measured by real-time RT-PCR. Expression levels were normalized to β-actin levels within the same sample. B , Protein expression of S100A14 and S100A16. Expression levels and subcellular localization were visualized using immunofluorescence staining. Scale bar; 50 μm. C , Subcellular localization of the S100A14 protein on the cell membrane. Z-axis images of confluent MCF7 cells were constructed using confocal laser scanning microscopy. Scale bar; 10 μm. D , Effect of omission of cell permeabilization on the immunofluorescent staining of S100A14. Following fixation with 4% paraformaldehyde, the cells were treated with or without 0.1% Triton X-100 in PBS prior to staining. Scale bar; 50 μm. E , Ca 2 -independent localization of S100A14 on the cell membrane. MCF7 cells transfected with the S100A14-GFP expression vector were observed by fluorescence microscopy over 180 min after addition of 10 mM EGTA in PBS. Scale bar; 50 μm.

    Journal: BMC Cancer

    Article Title: Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion

    doi: 10.1186/s12885-015-1059-6

    Figure Lengend Snippet: Expression and localization of S100A14 and S100A16 in human breast cancer cell lines. A , Relative mRNA expression levels measured by real-time RT-PCR. Expression levels were normalized to β-actin levels within the same sample. B , Protein expression of S100A14 and S100A16. Expression levels and subcellular localization were visualized using immunofluorescence staining. Scale bar; 50 μm. C , Subcellular localization of the S100A14 protein on the cell membrane. Z-axis images of confluent MCF7 cells were constructed using confocal laser scanning microscopy. Scale bar; 10 μm. D , Effect of omission of cell permeabilization on the immunofluorescent staining of S100A14. Following fixation with 4% paraformaldehyde, the cells were treated with or without 0.1% Triton X-100 in PBS prior to staining. Scale bar; 50 μm. E , Ca 2 -independent localization of S100A14 on the cell membrane. MCF7 cells transfected with the S100A14-GFP expression vector were observed by fluorescence microscopy over 180 min after addition of 10 mM EGTA in PBS. Scale bar; 50 μm.

    Article Snippet: Construction of S100A14 and S100A16 expression vectors and transfection cDNA expression vectors for the human S100A14 and S100A16 genes were constructed by PCR amplification of their coding regions using cDNAs derived from MCF7 cells as templates and specific primers, followed by cloning of the genes into a pEGFP expression vector (Takara-Clontech, Shiga, Japan).

    Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Construct, Confocal Laser Scanning Microscopy, Transfection, Plasmid Preparation, Fluorescence, Microscopy

    Morphological diversity of inflated calyx and expression of MPF2-like genes. A ) Photographs exhibiting diversity in the flowering and fruiting calyces of Withania , Tubocapsicum , Vassobia and Physalis . B ) Graph showing variations in flowering and fruiting calyces of Withania , Tubocapsicum , Vassobia and Physalis . Length and width of 10 calyces at different position of flowering and fruiting calyces were measured using a Vernier scale and size of calyx was calculated. Different accessions of Solanaceous plants are indicated on horizontal axis. Error bars indicate the standard deviations of the mean. C ) Scanning electron microscopy of Withania exhibits differences in the growth patterns of Withania and Tubocapsicum calyx epidermal cells surrounding berry. Note the increase and lobation of Withania cells in comparison with Tubocapsicum . Bars correspond to 20 mm. D ) Expression analysis of MPF2-like genes. The RNAs isolated from leaves, flower buds, sepals, stamens, carpels and siliques of Withania somnifera and Tubocapsicum anomalum were subjected to real-time RT-PCR analysis with gene specific primer pairs. The columns show the expression of MPF2-like-A of Withania ( WSA206 ; white), MPF2-like-B of Withania ( WSB206 ; reddish brown) and MPF2-like-B of Tubocapsicum ( TAB201 ; light blue). The values given are relative expression based on three independent experiments normalized with respect to 18 S rRNA . Error bars indicate the standard deviation.

    Journal: PLoS ONE

    Article Title: Reciprocal Loss of CArG-Boxes and Auxin Response Elements Drives Expression Divergence of MPF2-Like MADS-Box Genes Controlling Calyx Inflation

    doi: 10.1371/journal.pone.0042781

    Figure Lengend Snippet: Morphological diversity of inflated calyx and expression of MPF2-like genes. A ) Photographs exhibiting diversity in the flowering and fruiting calyces of Withania , Tubocapsicum , Vassobia and Physalis . B ) Graph showing variations in flowering and fruiting calyces of Withania , Tubocapsicum , Vassobia and Physalis . Length and width of 10 calyces at different position of flowering and fruiting calyces were measured using a Vernier scale and size of calyx was calculated. Different accessions of Solanaceous plants are indicated on horizontal axis. Error bars indicate the standard deviations of the mean. C ) Scanning electron microscopy of Withania exhibits differences in the growth patterns of Withania and Tubocapsicum calyx epidermal cells surrounding berry. Note the increase and lobation of Withania cells in comparison with Tubocapsicum . Bars correspond to 20 mm. D ) Expression analysis of MPF2-like genes. The RNAs isolated from leaves, flower buds, sepals, stamens, carpels and siliques of Withania somnifera and Tubocapsicum anomalum were subjected to real-time RT-PCR analysis with gene specific primer pairs. The columns show the expression of MPF2-like-A of Withania ( WSA206 ; white), MPF2-like-B of Withania ( WSB206 ; reddish brown) and MPF2-like-B of Tubocapsicum ( TAB201 ; light blue). The values given are relative expression based on three independent experiments normalized with respect to 18 S rRNA . Error bars indicate the standard deviation.

    Article Snippet: Their corresponding MPF2-like large 1st intron sequences were isolated by PCR amplification by using primers designed to bind to promoter region and second exon of MPF2-like genes ( ) with Takara LA Taq polymerase.

    Techniques: Expressing, Electron Microscopy, Isolation, Quantitative RT-PCR, Standard Deviation

    Phylogenetic reconstruction of MPF2-like genes and expression patterns of their progenitors. A ) A maximum likelihood tree of MPF2-like sequences from various Solanaceous species is established using STMADS16 as an out-group. The ML tree reconstruction is carried out with PAUP 4.0b10. The robustness of the tree structure is evaluated by 1000 replicates of bootstrap searches using maximum parsimony (MP) and maximum likelihood (ML) in PAUP and Bayesian posterior probability is indicated as *. The multiple sequences for a gene are also indicated. B ) The expression patterns of MPF2-like duplicates replicate their subsumed progenitors. RNA isolated from leaves and sepals of Physalis and Vassobia was subjected to real-time RT-PCR analysis. The values given for leaf (empty bar) and calyx (light grey bar) represent the relative expression normalized with respect to 18 S rRNA . Error bars indicate the standard deviation.

    Journal: PLoS ONE

    Article Title: Reciprocal Loss of CArG-Boxes and Auxin Response Elements Drives Expression Divergence of MPF2-Like MADS-Box Genes Controlling Calyx Inflation

    doi: 10.1371/journal.pone.0042781

    Figure Lengend Snippet: Phylogenetic reconstruction of MPF2-like genes and expression patterns of their progenitors. A ) A maximum likelihood tree of MPF2-like sequences from various Solanaceous species is established using STMADS16 as an out-group. The ML tree reconstruction is carried out with PAUP 4.0b10. The robustness of the tree structure is evaluated by 1000 replicates of bootstrap searches using maximum parsimony (MP) and maximum likelihood (ML) in PAUP and Bayesian posterior probability is indicated as *. The multiple sequences for a gene are also indicated. B ) The expression patterns of MPF2-like duplicates replicate their subsumed progenitors. RNA isolated from leaves and sepals of Physalis and Vassobia was subjected to real-time RT-PCR analysis. The values given for leaf (empty bar) and calyx (light grey bar) represent the relative expression normalized with respect to 18 S rRNA . Error bars indicate the standard deviation.

    Article Snippet: Their corresponding MPF2-like large 1st intron sequences were isolated by PCR amplification by using primers designed to bind to promoter region and second exon of MPF2-like genes ( ) with Takara LA Taq polymerase.

    Techniques: Expressing, Isolation, Quantitative RT-PCR, Standard Deviation