taq dna polymerase  (New England Biolabs)


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  • 98

    Structured Review

    New England Biolabs taq dna polymerase
    Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/New England Biolabs
    Average 98 stars, based on 1274 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-01
    98/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: Pre-PCR A multiplex pre-PCR step was included before PCR analysis in order to detect low expression miRNAs (Tang et al., ). miRNA-specific sense-primers and a universal anti-sense primer pairing to the cDNA stem-loop region were designed as described by Chen et al. (Chen et al., ; Tang et al., ; Schmittgen et al., ). .. Five microliters of the RT product was combined with 50 nM sense primer, 2 μM universal anti-sense primer, 5 μl of 5 × PCR buffer, 0.5 mM dNTP, and 0.25 U/μl Taq polymerase (NEB, cat. No M0324S) in a 25-μl total volume reaction.

    Amplification:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: Semi-quantitative RT-PCR Equal volumes of diluted pre-PCR product were combined with 1 μM each sense and anti-sense-primers, 3 μl of 5 × PCR buffer, 0.5 mM dNTP, and 1 U of Taq polymerase (NEB, cat. No M0324S) in a 15-μl total volume reaction. .. The PCR amplification was performed in the Biometra T1 plus thermocycler with the following cycling conditions: 95°C for 3 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. Final extension was carried out at 72°C for 10 min. PCR product was resolved by 20% polyacrylamide gel electrophoresis and stained with ethidium bromide for 45 min prior to image acquisition.

    Expressing:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: Pre-PCR A multiplex pre-PCR step was included before PCR analysis in order to detect low expression miRNAs (Tang et al., ). miRNA-specific sense-primers and a universal anti-sense primer pairing to the cDNA stem-loop region were designed as described by Chen et al. (Chen et al., ; Tang et al., ; Schmittgen et al., ). .. Five microliters of the RT product was combined with 50 nM sense primer, 2 μM universal anti-sense primer, 5 μl of 5 × PCR buffer, 0.5 mM dNTP, and 0.25 U/μl Taq polymerase (NEB, cat. No M0324S) in a 25-μl total volume reaction.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: .. Semi-quantitative RT-PCR Equal volumes of diluted pre-PCR product were combined with 1 μM each sense and anti-sense-primers, 3 μl of 5 × PCR buffer, 0.5 mM dNTP, and 1 U of Taq polymerase (NEB, cat. No M0324S) in a 15-μl total volume reaction. .. The PCR amplification was performed in the Biometra T1 plus thermocycler with the following cycling conditions: 95°C for 3 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. Final extension was carried out at 72°C for 10 min. PCR product was resolved by 20% polyacrylamide gel electrophoresis and stained with ethidium bromide for 45 min prior to image acquisition.

    Article Title: Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq strategy to profile poly(A+) RNA
    Article Snippet: SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies, cat. no. 18080-051), including 10× RT buffer, 25 mM MgCl2 , 0.1 M DTT, 10 mM dNTP mix, 200 U/μL SuperScript III RT, and 40 U/μL RNaseOUT. .. Taq DNA polymerase (NEB, cat. no. M0237L).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: Semi-quantitative RT-PCR Equal volumes of diluted pre-PCR product were combined with 1 μM each sense and anti-sense-primers, 3 μl of 5 × PCR buffer, 0.5 mM dNTP, and 1 U of Taq polymerase (NEB, cat. No M0324S) in a 15-μl total volume reaction. .. The PCR amplification was performed in the Biometra T1 plus thermocycler with the following cycling conditions: 95°C for 3 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. Final extension was carried out at 72°C for 10 min. PCR product was resolved by 20% polyacrylamide gel electrophoresis and stained with ethidium bromide for 45 min prior to image acquisition.

    Polymerase Chain Reaction:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: .. Semi-quantitative RT-PCR Equal volumes of diluted pre-PCR product were combined with 1 μM each sense and anti-sense-primers, 3 μl of 5 × PCR buffer, 0.5 mM dNTP, and 1 U of Taq polymerase (NEB, cat. No M0324S) in a 15-μl total volume reaction. .. The PCR amplification was performed in the Biometra T1 plus thermocycler with the following cycling conditions: 95°C for 3 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. Final extension was carried out at 72°C for 10 min. PCR product was resolved by 20% polyacrylamide gel electrophoresis and stained with ethidium bromide for 45 min prior to image acquisition.

    Article Title: Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq strategy to profile poly(A+) RNA
    Article Snippet: NucleoSpin gel and PCR clean-up (Macherey-Nagel, cat. no. 470609). .. Taq DNA polymerase (NEB, cat. no. M0237L).

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: .. Five microliters of the RT product was combined with 50 nM sense primer, 2 μM universal anti-sense primer, 5 μl of 5 × PCR buffer, 0.5 mM dNTP, and 0.25 U/μl Taq polymerase (NEB, cat. No M0324S) in a 25-μl total volume reaction. .. PCR cycling parameters were: 95°C for 10 min, 55°C for 2 min followed by 18 cycles of 95°C for 1 s and 65°C for 1 min.

    Staining:

    Article Title: Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes
    Article Snippet: Semi-quantitative RT-PCR Equal volumes of diluted pre-PCR product were combined with 1 μM each sense and anti-sense-primers, 3 μl of 5 × PCR buffer, 0.5 mM dNTP, and 1 U of Taq polymerase (NEB, cat. No M0324S) in a 15-μl total volume reaction. .. The PCR amplification was performed in the Biometra T1 plus thermocycler with the following cycling conditions: 95°C for 3 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s. Final extension was carried out at 72°C for 10 min. PCR product was resolved by 20% polyacrylamide gel electrophoresis and stained with ethidium bromide for 45 min prior to image acquisition.

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  • 90
    New England Biolabs taq pcr kit
    Taq Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq pcr kit/product/New England Biolabs
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    taq pcr kit - by Bioz Stars, 2020-01
    90/100 stars
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