taq dna polymerase  (Millipore)


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    Structured Review

    Millipore taq dna polymerase
    (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the <t>Taq</t> <t>DNA</t> polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.
    Taq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    taq dna polymerase - by Bioz Stars, 2020-01
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    1) Product Images from "Platinum nanoparticles induce damage to DNA and inhibit DNA replication"

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0180798

    (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the Taq DNA polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.
    Figure Legend Snippet: (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the Taq DNA polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.

    Techniques Used: Polymerase Chain Reaction, Binding Assay, Concentration Assay, Fluorescence, Sequencing

    Related Articles

    Clone Assay:

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: The current study used a cloned mutant allele (Additional file ) as mutant genotype. .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ].

    Centrifugation:

    Article Title: Isolation and Antibiogram of Clostridium tetani from Clinically Diagnosed Tetanus Patients
    Article Snippet: The resultant cell suspension was boiled for 10 minutes, followed by centrifugation to pellet the cell debris and obtain the supernatant containing the C. tetani DNA. .. Sample DNAs were added to a mixture containing PCR buffer, dNTPs, reverse and forward primers (20 μmol/L each), and Taq polymerase using the standard concentrations for a 50 μL reaction (Sigma, United Kingdom).

    Amplification:

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    Article Snippet: The forward and reverse primers for the amplification of λ xis gene fragment were synthesized by Sigma-Aldrich and their sequences were 5'-CCTGCTCTGCCGCTTCACGC-3' and 5'-TCCGGATAAAAACGTCGATGACATTTGC-3' , respectively. .. The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA.

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    Article Snippet: .. The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection. .. For analysis of expression after DSB induction or rapamycin addition, the data were normalized to the expression level in untreated control; expression of IPP1 (inorganic pyrophosphatase) was used as a reference, and relative expression in WT and tor1Δ cells was calculated using the ΔCt method.

    Synthesized:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
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    Article Title: C-deletion in exon 4 codon 63 of p53 gene as a molecular marker for oral squamous cell carcinoma: A preliminary study
    Article Snippet: Tris-HCl, Tris-buffer, sodium dodecyl sulfate, NaCl, KCl, dNTP, Taq DNA polymerase, ethidium bromide, agarose, and RNAase were obtained from Sigma Chemical Company, St. Louie, USA. .. The primers were synthesized and provided by Bengaluru Genei Company, India.

    Quantitative RT-PCR:

    Article Title: MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2
    Article Snippet: Paragraph title: RT-qPCR ... The PCR reaction system is as follows: 2.5 µl dNTP (2.5 mM each); 2.5 µl 10× PCR buffer; 1.5 µl MgCl2 solution; 1 U Taq polymerase; 0.25× SYBRGreen I (Sigma-Aldrich; Merck KGaA) (final concentration); 1 µl 10 µM PCR forward and reverse primer; 1 µl cDNA; water (to a total volume of 25 µl).

    SYBR Green Assay:

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: .. The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection. .. For analysis of expression after DSB induction or rapamycin addition, the data were normalized to the expression level in untreated control; expression of IPP1 (inorganic pyrophosphatase) was used as a reference, and relative expression in WT and tor1Δ cells was calculated using the ΔCt method.

    Incubation:

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: .. The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection. .. For analysis of expression after DSB induction or rapamycin addition, the data were normalized to the expression level in untreated control; expression of IPP1 (inorganic pyrophosphatase) was used as a reference, and relative expression in WT and tor1Δ cells was calculated using the ΔCt method.

    Expressing:

    Article Title: Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae
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    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: Paragraph title: RNA isolation and in vivo gene expression ... The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection.

    Modification:

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    Transformation Assay:

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    Activated Clotting Time Assay:

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    Article Snippet: Briefly, the Template for AA × 6 (5′- GGA GAG GGU UUA AUC AGC AGC AGC AGC AGC AGU ACG AAA GUA CAG CAG CAG CAG CAG CAG AUU GGA UCC GCA AGG - 3′) and complementary sequence (5′- GGC CGG ATC CTA ATA CGA CTC ACT ATA GGG AGA GGG TTT AAT - 3′) were procured from Sigma-Aldrich Chemicals Ltd. (St. Louis, MO, USA). .. The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands.

    Sequencing:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
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    Article Title: Discovery of a potent small molecule inhibiting Huntington’s disease (HD) pathogenesis via targeting CAG repeats RNA and Poly Q protein
    Article Snippet: Briefly, the Template for AA × 6 (5′- GGA GAG GGU UUA AUC AGC AGC AGC AGC AGC AGU ACG AAA GUA CAG CAG CAG CAG CAG CAG AUU GGA UCC GCA AGG - 3′) and complementary sequence (5′- GGC CGG ATC CTA ATA CGA CTC ACT ATA GGG AGA GGG TTT AAT - 3′) were procured from Sigma-Aldrich Chemicals Ltd. (St. Louis, MO, USA). .. The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands.

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: Methods In T-ARMS PCR, the interaction of primers is a complex phenomenon and may partially depend on its sequence. .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ].

    Article Title: MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2
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    Article Title: Next Generation Sequencing and Transcriptome Analysis Predicts Biosynthetic Pathway of Sennosides from Senna (Cassia angustifolia Vahl.), a Non-Model Plant with Potent Laxative Properties
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    Antiviral Assay:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: PCR was performed in a volume of 50 μl containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2O up to 50 μl. .. The PCR products were digested by three restriction enzymes of Ava II, Hph I, and Hpa II (Fermentas, Canada) as previously described [ ].

    In Vivo:

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: Paragraph title: RNA isolation and in vivo gene expression ... The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection.

    Real-time Polymerase Chain Reaction:

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: .. The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection. .. For analysis of expression after DSB induction or rapamycin addition, the data were normalized to the expression level in untreated control; expression of IPP1 (inorganic pyrophosphatase) was used as a reference, and relative expression in WT and tor1Δ cells was calculated using the ΔCt method.

    Fluorescence:

    Article Title: MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2
    Article Snippet: The reactions were performed on a fluorescence RT-qPCR instrument, according to the manufacturer's protocol. .. The PCR reaction system is as follows: 2.5 µl dNTP (2.5 mM each); 2.5 µl 10× PCR buffer; 1.5 µl MgCl2 solution; 1 U Taq polymerase; 0.25× SYBRGreen I (Sigma-Aldrich; Merck KGaA) (final concentration); 1 µl 10 µM PCR forward and reverse primer; 1 µl cDNA; water (to a total volume of 25 µl).

    Mutagenesis:

    Article Title: Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae
    Article Snippet: The Agrocybe aegerita UPO secretion mutant (PaDa-I) was obtained as described elsewhere ( ). .. ABTS, DMP, veratryl alcohol, benzyl alcohol, Tween-20, hemoglobin from bovine blood, ascorbic acid, anthracene, Taq DNA polymerase, and a yeast transformation kit were purchased from Sigma-Aldrich (Madrid, Spain).

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: The current study used a cloned mutant allele (Additional file ) as mutant genotype. .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ].

    Isolation:

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: Paragraph title: RNA isolation and in vivo gene expression ... The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection.

    Size-exclusion Chromatography:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: The composition of PCR mixture (50 μl) consisted of 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 20ng DNA template and Millipore H2O up to 50 μl. .. The amplification conditions using PCR thermocycler (BIORAD, USA) were as follows: initial denaturation at 95°C for 5 min followed by 35 cycles of denaturation at 94°C for 15 sec, annealing at 58°C for 15 sec, and extension at 72°C for 30 sec and final extension at 72°C for 10 min. M. tuberculosis H37Rv, M. bovis BCG and M. kansasi (DSM44162) were used as control positive.

    Article Title: MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2
    Article Snippet: The PCR reaction system is as follows: 2.5 µl dNTP (2.5 mM each); 2.5 µl 10× PCR buffer; 1.5 µl MgCl2 solution; 1 U Taq polymerase; 0.25× SYBRGreen I (Sigma-Aldrich; Merck KGaA) (final concentration); 1 µl 10 µM PCR forward and reverse primer; 1 µl cDNA; water (to a total volume of 25 µl). .. The U6 reaction was as follows: 95°C for 5 min and 35 cycles of 95°C for 10 sec, 59°C for 15 sec, 72°C for 20 sec and 82°C for 5 sec.

    Purification:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA. .. The obtained DNA fragments (498 bp) were purified by MinElute PCR Purification Kit (Qiagen, Germany).

    Polymerase Chain Reaction:

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication
    Article Snippet: Paragraph title: Polymerase chain reaction (PCR) ... The volume of the reaction mixture was 25 μL, which was composed of 2.5 μL of 10× standard Taq reaction buffer, 0.5 μL of 1 mM deoxynucleotide solution, 0.5 μL of each of the primers (10 μM), 0.125 μL of Taq DNA polymerase; selected volume of water or drugs diluted with water (sterile, ACS purity, Sigma-Aldrich) and 0.5 μL of bacteriophage λ DNA.

    Article Title: A Mariner Transposon-Based Signature-Tagged Mutagenesis System for the Analysis of Oral Infection by Listeria monocytogenes
    Article Snippet: .. DNA was amplified from either end of the transposon with a series of two rounds of PCR with Taq polymerase in the first round and KOD High Fidelity polymerase (Novagen) in the second round. ..

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: .. PCR was performed in a volume of 50 μl containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2O up to 50 μl. ..

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: .. The composition of PCR mixture (50 μl) consisted of 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 20ng DNA template and Millipore H2O up to 50 μl. .. The amplification conditions using PCR thermocycler (BIORAD, USA) were as follows: initial denaturation at 95°C for 5 min followed by 35 cycles of denaturation at 94°C for 15 sec, annealing at 58°C for 15 sec, and extension at 72°C for 30 sec and final extension at 72°C for 10 min. M. tuberculosis H37Rv, M. bovis BCG and M. kansasi (DSM44162) were used as control positive.

    Article Title: Discovery of a potent small molecule inhibiting Huntington’s disease (HD) pathogenesis via targeting CAG repeats RNA and Poly Q protein
    Article Snippet: .. The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands. .. The amplified products were mixed with 6X DNA loading dye and resolved on 3% agarose gel stained with ethidium bromide.

    Article Title: Isolation and Antibiogram of Clostridium tetani from Clinically Diagnosed Tetanus Patients
    Article Snippet: .. Sample DNAs were added to a mixture containing PCR buffer, dNTPs, reverse and forward primers (20 μmol/L each), and Taq polymerase using the standard concentrations for a 50 μL reaction (Sigma, United Kingdom). .. The resulting PCR amplicons were examined on a 1% agarose gel with ethidium bromide.

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Article Title: Next Generation Sequencing and Transcriptome Analysis Predicts Biosynthetic Pathway of Sennosides from Senna (Cassia angustifolia Vahl.), a Non-Model Plant with Potent Laxative Properties
    Article Snippet: .. Each PCR reaction mixture contained 2–4 pmol of primer, 1–4 mM MgCl2 , 0.1–0.2 mM dNTP, 0.75 U Taq DNA polymerase and 1.5µl 10x PCR buffer (Sigma–Aldrich, St. Louis, MO, USA) and 30–50 ng of genomic DNA as a template. .. Temperature cycling was carried out using the S1000 Thermal Cycler (Bio-Rad Laboratories, Philadelphia, PA, USA) and touch-down PCR amplification [ ]: one 15-min denaturation cycle, followed by ten cycles of 94° C for 10 s, 61° C for 20 s (reducing by 1° C per cycle) and 72° C for 30 s, then by 31 cycles of 94° C for 10 s, 54° C for 20 s and 72° C for 30 s. After completion of the 31 cycles, a final extension of 20 min at 72° C was included to minimize the +A overhang [ ].

    Article Title: DNA damage regulates direct association of TOR kinase with the RNA polymerase II–transcribed HMO1 gene
    Article Snippet: Contaminating DNA was removed using Turbo DNase (Ambion), and absence of DNA was verified by PCR. .. The mixture was incubated at 42°C for 1 h. A ViiA 7 (Applied Biosystems) was used for quantitative PCR (qPCR) using Taq polymerase for amplification and SYBR Green I (Sigma) for detection.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Titration:

    Article Title: Discovery of a potent small molecule inhibiting Huntington’s disease (HD) pathogenesis via targeting CAG repeats RNA and Poly Q protein
    Article Snippet: .. The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands. .. The amplified products were mixed with 6X DNA loading dye and resolved on 3% agarose gel stained with ethidium bromide.

    Plasmid Preparation:

    Article Title: Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae
    Article Snippet: The expression shuttle vector pJRoC30, with uracil auxotrophy and an ampicillin marker for selection, came from the California Institute of Technology (USA). .. ABTS, DMP, veratryl alcohol, benzyl alcohol, Tween-20, hemoglobin from bovine blood, ascorbic acid, anthracene, Taq DNA polymerase, and a yeast transformation kit were purchased from Sigma-Aldrich (Madrid, Spain).

    Article Title: Evaluation of Recombinant Multi-Epitope Outer Membrane Protein-Based Klebsiella pneumoniae Subunit Vaccine in Mouse Model
    Article Snippet: Taq DNA polymerase, dNTPs, IPTG and adjuvants were from Sigma–Aldrich, India. .. Pfu DNA polymerase was from Fermentas, United States. pRSET vector series, Escherichia coli DH5α and BL21DE3 strains are from Invitrogen, United States.

    Software:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: In brief, the reaction mixture were performed in a volume of 50 μl containing 20 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200mM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2 O up to 50 μl. .. In brief, the reaction mixture were performed in a volume of 50 μl containing 20 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200mM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2 O up to 50 μl.

    Electrophoresis:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: In brief, the reaction mixture were performed in a volume of 50 μl containing 20 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200mM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2 O up to 50 μl. .. The programming conditions were as initial denaturation at 94°C for 2 min, followed by 35 cycles of denaturation at 94°C for 45s, annealing at 52°C for 1 min and extension at 70°C for 1 min, with a final extension at 72°C for 10 min. Amplified products were separated by electrophoresis in 2% w/v agarose gels after staining with ethidium bromide.

    Selection:

    Article Title: Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae
    Article Snippet: The expression shuttle vector pJRoC30, with uracil auxotrophy and an ampicillin marker for selection, came from the California Institute of Technology (USA). .. ABTS, DMP, veratryl alcohol, benzyl alcohol, Tween-20, hemoglobin from bovine blood, ascorbic acid, anthracene, Taq DNA polymerase, and a yeast transformation kit were purchased from Sigma-Aldrich (Madrid, Spain).

    Agarose Gel Electrophoresis:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: PCR was performed in a volume of 50 μl containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2O up to 50 μl. .. Digested products were electrophoresed in 3% w/v agarose gel, stained with ethidium bromide and were visualized using gel documentation system (UV Tech, UK).

    Article Title: Discovery of a potent small molecule inhibiting Huntington’s disease (HD) pathogenesis via targeting CAG repeats RNA and Poly Q protein
    Article Snippet: The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands. .. The amplified products were mixed with 6X DNA loading dye and resolved on 3% agarose gel stained with ethidium bromide.

    Article Title: Isolation and Antibiogram of Clostridium tetani from Clinically Diagnosed Tetanus Patients
    Article Snippet: Sample DNAs were added to a mixture containing PCR buffer, dNTPs, reverse and forward primers (20 μmol/L each), and Taq polymerase using the standard concentrations for a 50 μL reaction (Sigma, United Kingdom). .. The resulting PCR amplicons were examined on a 1% agarose gel with ethidium bromide.

    Concentration Assay:

    Article Title: MicroRNA-206 regulates the epithelial-mesenchymal transition and inhibits the invasion and metastasis of prostate cancer cells by targeting Annexin A2
    Article Snippet: .. The PCR reaction system is as follows: 2.5 µl dNTP (2.5 mM each); 2.5 µl 10× PCR buffer; 1.5 µl MgCl2 solution; 1 U Taq polymerase; 0.25× SYBRGreen I (Sigma-Aldrich; Merck KGaA) (final concentration); 1 µl 10 µM PCR forward and reverse primer; 1 µl cDNA; water (to a total volume of 25 µl). .. The U6 reaction was as follows: 95°C for 5 min and 35 cycles of 95°C for 10 sec, 59°C for 15 sec, 72°C for 20 sec and 82°C for 5 sec.

    Marker:

    Article Title: Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae
    Article Snippet: The expression shuttle vector pJRoC30, with uracil auxotrophy and an ampicillin marker for selection, came from the California Institute of Technology (USA). .. ABTS, DMP, veratryl alcohol, benzyl alcohol, Tween-20, hemoglobin from bovine blood, ascorbic acid, anthracene, Taq DNA polymerase, and a yeast transformation kit were purchased from Sigma-Aldrich (Madrid, Spain).

    Staining:

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: PCR was performed in a volume of 50 μl containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200μM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2O up to 50 μl. .. Digested products were electrophoresed in 3% w/v agarose gel, stained with ethidium bromide and were visualized using gel documentation system (UV Tech, UK).

    Article Title: Discovery of a potent small molecule inhibiting Huntington’s disease (HD) pathogenesis via targeting CAG repeats RNA and Poly Q protein
    Article Snippet: The reaction was performed in a master mix consisting 1X PCR buffer, 0.33 mM dNTPs, 4.25 mM MgCl2 , 2 μM oligonucleotide, 2.5 units Taq DNA polymerase (Sigma-Aldrich Chemicals Ltd. St. Louis, MO, USA) and a dose of titration of ligands. .. The amplified products were mixed with 6X DNA loading dye and resolved on 3% agarose gel stained with ethidium bromide.

    Article Title: Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR
    Article Snippet: In brief, the reaction mixture were performed in a volume of 50 μl containing 20 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200mM dNTPs, 10pmol of each primer, 0.5U of Taq DNA polymerase and 50ng DNA template and Millipore H2 O up to 50 μl. .. The programming conditions were as initial denaturation at 94°C for 2 min, followed by 35 cycles of denaturation at 94°C for 45s, annealing at 52°C for 1 min and extension at 70°C for 1 min, with a final extension at 72°C for 10 min. Amplified products were separated by electrophoresis in 2% w/v agarose gels after staining with ethidium bromide.

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