taq dna polymerase  (Millipore)


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    Structured Review

    Millipore taq dna polymerase
    (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the <t>Taq</t> <t>DNA</t> polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.
    Taq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Millipore
    Average 96 stars, based on 577 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Platinum nanoparticles induce damage to DNA and inhibit DNA replication"

    Article Title: Platinum nanoparticles induce damage to DNA and inhibit DNA replication

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0180798

    (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the Taq DNA polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.
    Figure Legend Snippet: (A) The suggested effects of PtNPs on polymerase chain reaction (PCR) is based on binding of PtNPs to the Taq DNA polymerase, which leads to ceasing of PCR, (B) whereas CisPt primarily intercalates in DNA structure and stops PCR by this way. The gel electrophoregrams of PCR product mixture with particular concentration of (C) PtNPs (0.04–4 200 ng/mL of Pt) and (D) CisPt (0.04–42 000 ng/mL of Pt). (E) DNA denaturation temperature affected by the 0–200 μg/mL of Pt derivatives. Fluorescence of labelled nucleotides of DNA fragment after sequencing, which was influenced by (F) 0–20 μg/mL of PtNPs and (G) 0–0.33 μg/mL of CisPt. For all measurement n = 3.

    Techniques Used: Polymerase Chain Reaction, Binding Assay, Concentration Assay, Fluorescence, Sequencing

    2) Product Images from "Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins"

    Article Title: Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

    Journal: BMC Biotechnology

    doi: 10.1186/s12896-016-0316-3

    Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I
    Figure Legend Snippet: Performance of four thermostable DNA polymerases for the synthesis of gene B using PCA-DTF. Lane 1: negative control reaction performed at the same conditions, without addition of primers; lane 2: KOD Hot Start DNA polymerase; lane 3: Q5 Hot Start High Fidelity DNA polymerase; lane 4: Pfu Turbo DNA polymerase and lane 5: Taq DNA polymerase. M2: NZYladder I

    Techniques Used: Negative Control

    Related Articles

    Amplification:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Chromatography:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Purification:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Concentration Assay:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Generated:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Polymerase Chain Reaction:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Plasmid Preparation:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis
    Article Snippet: .. GenElute™ Plasmid Miniprep Kit, Lysozyme from chicken egg white, dNTP Mix, Taq DNA Polymerase, ampicillin, kanamycin sulphate, and albumin from bovine serum (BSA) were purchased from Sigma-Aldrich, USA. .. Antigen of RBPT was obtained from Pendik Veterinary Control and Research Institute.

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    Millipore one step rt pcr master mix kit
    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the <t>EMD</t> Millipore NSP3 <t>qRT-PCR</t> assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
    One Step Rt Pcr Master Mix Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one step rt pcr master mix kit/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    one step rt pcr master mix kit - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Millipore pcr
    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the <t>EMD</t> Millipore NSP3 <t>qRT-PCR</t> assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
    Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Millipore
    Average 99 stars, based on 11792 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the EMD Millipore NSP3 qRT-PCR assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of an Alternative Recombinant Thermostable Thermus thermophilus (rTth)-Based Real-Time Reverse Transcription-PCR Kit for Detection of Rotavirus A

    doi: 10.1128/JCM.00126-17

    Figure Lengend Snippet: Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the EMD Millipore NSP3 qRT-PCR assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.

    Article Snippet: Here, we report evaluation of a one-step RT-PCR master mix kit (EMD Millipore Corporation, Billerica, MA, USA) for detection of the RVA NSP3 gene.

    Techniques: Amplification, Quantitative RT-PCR