taq dna polymerase  (Jena Bioscience)


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    Structured Review

    Jena Bioscience taq dna polymerase
    Taq Dna Polymerase, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Jena Bioscience
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
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    Related Articles

    Modification:

    Article Title: Differentiation of Campylobacter species by surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry.
    Article Snippet: .. The genus Campylobacter contains several, widespread pathogens causing food-borne diseases of zoonotic nature in humans. .. The genus Campylobacter contains several, widespread pathogens causing food-borne diseases of zoonotic nature in humans.

    Polymerase Chain Reaction:

    Article Title: A signal amplification probe enhances sensitivity of antibodies and aptamers based Immuno-diagnostic assays.
    Article Snippet: .. One major unmet need is improving the sensitivity of immune-diagnostic assays. .. One major unmet need is improving the sensitivity of immune-diagnostic assays.

    Article Title: Cyanotrophic and arsenic oxidizing activities of Pseudomonas mendocina P6115 isolated from mine tailings containing high cyanide concentration.
    Article Snippet: .. Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations. .. Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations.

    Article Title: Prevalence of CYP2C19 alleles, pharmacokinetic and pharmacodynamic variation of clopidogrel and prasugrel in Bangladeshi population.
    Article Snippet: .. The extent to which cytochrome P450 (CYP) 2C19 genotype influences the effectiveness of clopidogrel remains uncertain due to considerable heterogeneity between studies. .. The extent to which cytochrome P450 (CYP) 2C19 genotype influences the effectiveness of clopidogrel remains uncertain due to considerable heterogeneity between studies.

    Article Title: Molecular detection of ochratoxigenic Aspergillus species isolated from coffee beans in Saudi Arabia.
    Article Snippet: .. All amplification reactions were carried out in a final volume of 25 µL containing 15 ng template DNA, 20 pmol of each primer, 1X PCR buffer, 2 µM MgCl2, 1 mM dNTPs and 0.04 U/µL Taq DNA polymerase (Jena-Bioscience). .. PCRs were performed in a Techne TC-312 (Techne) PCR system.

    Article Title: An efficient method for DNA extraction from Cladosporioid fungi.
    Article Snippet: .. Universally primed PCR Amplification reactions were performed in 0.2-mL microcentrifuge tubes in a 25-µL reaction volume containing 10 mM Tris-HCl, pH 8.8, 50 mM KCl, 0.8 mM NaCl, 3.5 mM MgCl2, 0.1% Triton X-100, 0.4 mM dNTPs, 20 pmol for primer L21 or AS4, 1.0 U Taq DNA polymerase (JenaBioscience) and 10 to 15 ng genomic DNA. .. PCR amplification was performed in a C1000 Thermal Cycler (Bio-Rad Laboratories, CA, USA) programmed for 30 cycles of denaturation at 94°C for 30 s (first denaturation step at 94°C for 3 min), annealing at 56°C for 70 s and polymerization at 72°C for 60 s, with a final extension step of 72°C for 5 min.

    Amplification:

    Article Title: Prevalence of CYP2C19 alleles, pharmacokinetic and pharmacodynamic variation of clopidogrel and prasugrel in Bangladeshi population.
    Article Snippet: .. The extent to which cytochrome P450 (CYP) 2C19 genotype influences the effectiveness of clopidogrel remains uncertain due to considerable heterogeneity between studies. .. The extent to which cytochrome P450 (CYP) 2C19 genotype influences the effectiveness of clopidogrel remains uncertain due to considerable heterogeneity between studies.

    Article Title: Molecular detection of ochratoxigenic Aspergillus species isolated from coffee beans in Saudi Arabia.
    Article Snippet: .. All amplification reactions were carried out in a final volume of 25 µL containing 15 ng template DNA, 20 pmol of each primer, 1X PCR buffer, 2 µM MgCl2, 1 mM dNTPs and 0.04 U/µL Taq DNA polymerase (Jena-Bioscience). .. PCRs were performed in a Techne TC-312 (Techne) PCR system.

    Article Title: An efficient method for DNA extraction from Cladosporioid fungi.
    Article Snippet: .. Universally primed PCR Amplification reactions were performed in 0.2-mL microcentrifuge tubes in a 25-µL reaction volume containing 10 mM Tris-HCl, pH 8.8, 50 mM KCl, 0.8 mM NaCl, 3.5 mM MgCl2, 0.1% Triton X-100, 0.4 mM dNTPs, 20 pmol for primer L21 or AS4, 1.0 U Taq DNA polymerase (JenaBioscience) and 10 to 15 ng genomic DNA. .. PCR amplification was performed in a C1000 Thermal Cycler (Bio-Rad Laboratories, CA, USA) programmed for 30 cycles of denaturation at 94°C for 30 s (first denaturation step at 94°C for 3 min), annealing at 56°C for 70 s and polymerization at 72°C for 60 s, with a final extension step of 72°C for 5 min.

    Synthesized:

    Article Title: A signal amplification probe enhances sensitivity of antibodies and aptamers based Immuno-diagnostic assays.
    Article Snippet: .. One major unmet need is improving the sensitivity of immune-diagnostic assays. .. One major unmet need is improving the sensitivity of immune-diagnostic assays.

    Isolation:

    Article Title: An efficient method for DNA extraction from Cladosporioid fungi.
    Article Snippet: .. This further confirmed that the isolated DNA was free of polysaccharide and polyphenols, which are known to inhibit Taq DNA polymerase and restriction endonucleases (Moyo et al., 2008). .. RNA can be removed by incubation with RNase A, either after the nucleic acids have been extracted or by inclusion in the extraction buffer (Abd-Elsalam et al., 2007).

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    Jena Bioscience cy5 modified
    Details of the ISD assay. ( a ) Intercalation induced Supercoiling of DNA- Schematics for the preparation of the intercalation-induced supercoiled DNA. A doubly biotinylated DNA at the ends is flowed along a streptavidin-coated surface at a constant flow velocity. One end of the DNA first binds to the surface via biotin-streptavidin interaction, which is followed by stretching of the molecule along the flow. The other end of the DNA then binds to surface resulting in a torsionally constrained DNA. Upon addition of an intercalating dye (Sytox Orange), DNA becomes supercoiled due to local unwinding induced by intercalation. Inset at the bottom panel, Schematics showing local unwinding of stacked base pairs due to intercalation of a dye molecule. In the B-form DNA structure, a pair (orange) of stacked bases make an angle of 34° with the next pair (green) and is separated by 0.34 nm. Intercalation of a dye molecule between the stacked bases increases the separation and decreases the angle, resulting in local unwinding of the DNA, which adds positive supercoiling to the rotationally constrained DNA molecule. ( b ) Schematics for DNA template preparation. Two plasmids (pCR-XL 12.5 kb and 14.7 kb) were expressed in methylation-free E. coli cells using a midiprep kit. Each plasmid contains two BsaI sites. The plasmids were first PCR-amplified and then digested with BsaI endonuclease to obtain the required sticky ends. An 8.4 kb DNA fragment was obtained from the 12.5 kb plasmid and a 11.2 kb DNA fragment from the 14.7 kb plasmid. The 8.4 kb fragment, a sequence of interest, and <t>biotin-Cy5-DNA</t> handle were ligated together. At the same time, the 11.2 kb fragment is ligated with biotin-DNA handle. The ligated fragments were then agarose gel-purified and ligated together. The final ligation product is purified by agarose gel electrophoresis. In the case of template 1, we skipped the sequence of interest and the 8.4 kb and 11.2 kb fragments were directly ligated together. Template two was obtained by digesting the single 21 kb plasmid and ligating with biotin handle at one end and biotin/Cy5 handle at the other end. ( c ) Fluorescence snap-shot showing several supercoiled DNA molecules. Yellow arrows point to the plectonemes on supercoiled DNA.
    Cy5 Modified, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
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    93
    Jena Bioscience fluorescein 12 dutp
    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with <t>fluorescein-12-dUTP</t> (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.
    Fluorescein 12 Dutp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jena Bioscience c8 alkyne dctp
    Effect of nucleotides on dNTP quantification by click assay. ( a – c ) Standard curves for ( a ) dATP, ( b ) <t>dCTP,</t> ( c ) dGTP were performed without- or with 100 pmol of dTTP and using Taq polymerase. 5-TAMRA-labeling of streptavidin Sepharose was determined by fluorescence quantitation. The red circle at the 5′ end of the primer indicates biotin. ( d ) Recovery of 20 pmol of dTTP analyzed by <t>C8-alkyne-dCTP</t> incorporation by Vent (exo-) polymerase in the presence of 50 pmol dCTP or 100 pmol dCTP. ( e ) Recovery of 10 pmol of dCTP and 20 pmol of dTTP were analyzed in the presence of rNTP mixtures (5000 pmol of each ATP, CTP, GTP and UTP). Bars represent mean ± SEM and assays were performed in triplicate.
    C8 Alkyne Dctp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c8 alkyne dctp/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Details of the ISD assay. ( a ) Intercalation induced Supercoiling of DNA- Schematics for the preparation of the intercalation-induced supercoiled DNA. A doubly biotinylated DNA at the ends is flowed along a streptavidin-coated surface at a constant flow velocity. One end of the DNA first binds to the surface via biotin-streptavidin interaction, which is followed by stretching of the molecule along the flow. The other end of the DNA then binds to surface resulting in a torsionally constrained DNA. Upon addition of an intercalating dye (Sytox Orange), DNA becomes supercoiled due to local unwinding induced by intercalation. Inset at the bottom panel, Schematics showing local unwinding of stacked base pairs due to intercalation of a dye molecule. In the B-form DNA structure, a pair (orange) of stacked bases make an angle of 34° with the next pair (green) and is separated by 0.34 nm. Intercalation of a dye molecule between the stacked bases increases the separation and decreases the angle, resulting in local unwinding of the DNA, which adds positive supercoiling to the rotationally constrained DNA molecule. ( b ) Schematics for DNA template preparation. Two plasmids (pCR-XL 12.5 kb and 14.7 kb) were expressed in methylation-free E. coli cells using a midiprep kit. Each plasmid contains two BsaI sites. The plasmids were first PCR-amplified and then digested with BsaI endonuclease to obtain the required sticky ends. An 8.4 kb DNA fragment was obtained from the 12.5 kb plasmid and a 11.2 kb DNA fragment from the 14.7 kb plasmid. The 8.4 kb fragment, a sequence of interest, and biotin-Cy5-DNA handle were ligated together. At the same time, the 11.2 kb fragment is ligated with biotin-DNA handle. The ligated fragments were then agarose gel-purified and ligated together. The final ligation product is purified by agarose gel electrophoresis. In the case of template 1, we skipped the sequence of interest and the 8.4 kb and 11.2 kb fragments were directly ligated together. Template two was obtained by digesting the single 21 kb plasmid and ligating with biotin handle at one end and biotin/Cy5 handle at the other end. ( c ) Fluorescence snap-shot showing several supercoiled DNA molecules. Yellow arrows point to the plectonemes on supercoiled DNA.

    Journal: eLife

    Article Title: DNA sequence encodes the position of DNA supercoils

    doi: 10.7554/eLife.36557

    Figure Lengend Snippet: Details of the ISD assay. ( a ) Intercalation induced Supercoiling of DNA- Schematics for the preparation of the intercalation-induced supercoiled DNA. A doubly biotinylated DNA at the ends is flowed along a streptavidin-coated surface at a constant flow velocity. One end of the DNA first binds to the surface via biotin-streptavidin interaction, which is followed by stretching of the molecule along the flow. The other end of the DNA then binds to surface resulting in a torsionally constrained DNA. Upon addition of an intercalating dye (Sytox Orange), DNA becomes supercoiled due to local unwinding induced by intercalation. Inset at the bottom panel, Schematics showing local unwinding of stacked base pairs due to intercalation of a dye molecule. In the B-form DNA structure, a pair (orange) of stacked bases make an angle of 34° with the next pair (green) and is separated by 0.34 nm. Intercalation of a dye molecule between the stacked bases increases the separation and decreases the angle, resulting in local unwinding of the DNA, which adds positive supercoiling to the rotationally constrained DNA molecule. ( b ) Schematics for DNA template preparation. Two plasmids (pCR-XL 12.5 kb and 14.7 kb) were expressed in methylation-free E. coli cells using a midiprep kit. Each plasmid contains two BsaI sites. The plasmids were first PCR-amplified and then digested with BsaI endonuclease to obtain the required sticky ends. An 8.4 kb DNA fragment was obtained from the 12.5 kb plasmid and a 11.2 kb DNA fragment from the 14.7 kb plasmid. The 8.4 kb fragment, a sequence of interest, and biotin-Cy5-DNA handle were ligated together. At the same time, the 11.2 kb fragment is ligated with biotin-DNA handle. The ligated fragments were then agarose gel-purified and ligated together. The final ligation product is purified by agarose gel electrophoresis. In the case of template 1, we skipped the sequence of interest and the 8.4 kb and 11.2 kb fragments were directly ligated together. Template two was obtained by digesting the single 21 kb plasmid and ligating with biotin handle at one end and biotin/Cy5 handle at the other end. ( c ) Fluorescence snap-shot showing several supercoiled DNA molecules. Yellow arrows point to the plectonemes on supercoiled DNA.

    Article Snippet: The ‘Cy5-biotin handle’ and ‘biotin handle’ were prepared by PCR methods in the presence of Cy5-modified and/or biotinylated dUTP (aminoallyl-dUTP-Cy5 and biotin-16-dUTP, Jena Bioscience).

    Techniques: Flow Cytometry, Polymerase Chain Reaction, Methylation, Plasmid Preparation, Amplification, Sequencing, Agarose Gel Electrophoresis, Purification, Ligation, Fluorescence

    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Journal: eLife

    Article Title: DNA sequence encodes the position of DNA supercoils

    doi: 10.7554/eLife.36557

    Figure Lengend Snippet: Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Article Snippet: The ‘Cy5-biotin handle’ and ‘biotin handle’ were prepared by PCR methods in the presence of Cy5-modified and/or biotinylated dUTP (aminoallyl-dUTP-Cy5 and biotin-16-dUTP, Jena Bioscience).

    Techniques: Flow Cytometry, Labeling, Binding Assay, Fluorescence, Mass Spectrometry

    Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Insights into the Structure of the Spruce Budworm (Choristoneura fumiferana) Genome, as Revealed by Molecular Cytogenetic Analyses and a High-Density Linkage Map

    doi: 10.1534/g3.118.200263

    Figure Lengend Snippet: Genomic in situ hybridization combined with the (TTAGG) n telomeric probe in pachytene chromosomes in male (a-c) and female (d-f) Choristoneura fumiferana . Female-derived genomic probe was labeled with fluorescein-12-dUTP (green), and the telomeric probe with Cy3-dUTP (red); chromosomes were counterstained with DAPI (blue). Panels (a-c) show a male pachytene complement; arrows indicate heterochromatic block highlighted with the female genomic probe. Panels (d-f) show a female pachytene complement; “WZ” label identifies the sex chromosome bivalent (see schematic drawing in the lower left corner of panel d), where discrimination of the W chromosome is provided by the female-derived genomic probe; “N” indicates a nucleolus associated with a heterochromatic region (showing strong hybridization signals of the female genomic probe in panel d-f) of an autosome bivalent. (a, d) Merged images of preparations hybridized with female-derived genomic probe and telomeric probe, and counterstained with DAPI; (b, e) DAPI staining pattern; (c, f) hybridization pattern obtained using female-derived genomic probe. Scale bars = 10 μm.

    Article Snippet: Female gDNA was labeled with fluorescein-12-dUTP (Jena Bioscience) using the Nick Translation Kit (Abbott Molecular Inc.), with 4 h of incubation at 15°, and male gDNA was sonicated using a Sonopuls HD 2070 (Bandelin Electric, Berlin, Germany) and used as competitor DNA.

    Techniques: In Situ Hybridization, Derivative Assay, Labeling, Blocking Assay, Hybridization, Staining

    Effect of nucleotides on dNTP quantification by click assay. ( a – c ) Standard curves for ( a ) dATP, ( b ) dCTP, ( c ) dGTP were performed without- or with 100 pmol of dTTP and using Taq polymerase. 5-TAMRA-labeling of streptavidin Sepharose was determined by fluorescence quantitation. The red circle at the 5′ end of the primer indicates biotin. ( d ) Recovery of 20 pmol of dTTP analyzed by C8-alkyne-dCTP incorporation by Vent (exo-) polymerase in the presence of 50 pmol dCTP or 100 pmol dCTP. ( e ) Recovery of 10 pmol of dCTP and 20 pmol of dTTP were analyzed in the presence of rNTP mixtures (5000 pmol of each ATP, CTP, GTP and UTP). Bars represent mean ± SEM and assays were performed in triplicate.

    Journal: Scientific Reports

    Article Title: Quantitation of deoxynucleoside triphosphates by click reactions

    doi: 10.1038/s41598-020-57463-3

    Figure Lengend Snippet: Effect of nucleotides on dNTP quantification by click assay. ( a – c ) Standard curves for ( a ) dATP, ( b ) dCTP, ( c ) dGTP were performed without- or with 100 pmol of dTTP and using Taq polymerase. 5-TAMRA-labeling of streptavidin Sepharose was determined by fluorescence quantitation. The red circle at the 5′ end of the primer indicates biotin. ( d ) Recovery of 20 pmol of dTTP analyzed by C8-alkyne-dCTP incorporation by Vent (exo-) polymerase in the presence of 50 pmol dCTP or 100 pmol dCTP. ( e ) Recovery of 10 pmol of dCTP and 20 pmol of dTTP were analyzed in the presence of rNTP mixtures (5000 pmol of each ATP, CTP, GTP and UTP). Bars represent mean ± SEM and assays were performed in triplicate.

    Article Snippet: C8-alkyne-dCTP and 5-ethynyl-dUTP were purchased from Jena Bioscience or Baseclick.

    Techniques: Labeling, Fluorescence, Quantitation Assay

    Standard curves for dNTP measurements. Templates as indicated were incubated with Zgene Taq polymerase in the presence of increasing amounts of ( a ) dCTP, ( b ) dATP and ( c ) dGTP together with EdUTP as described in Materials and Methods. ( d ) For dTTP measurement, templates were incubated with Vent (exo-) in the presence of known amounts of dTTP and C8-Alkyne-dCTP. The oligonucleotides pull-down using streptavidin Sepharose were labeled with 5-TAMRA-azide by click reaction. Fluorescence units of control reactions without dNTPs were subtracted from the values obtained in order to give normalized fluorescence units (NFU). ( e ) The chemical structure of C8-alkyne-dCTP.

    Journal: Scientific Reports

    Article Title: Quantitation of deoxynucleoside triphosphates by click reactions

    doi: 10.1038/s41598-020-57463-3

    Figure Lengend Snippet: Standard curves for dNTP measurements. Templates as indicated were incubated with Zgene Taq polymerase in the presence of increasing amounts of ( a ) dCTP, ( b ) dATP and ( c ) dGTP together with EdUTP as described in Materials and Methods. ( d ) For dTTP measurement, templates were incubated with Vent (exo-) in the presence of known amounts of dTTP and C8-Alkyne-dCTP. The oligonucleotides pull-down using streptavidin Sepharose were labeled with 5-TAMRA-azide by click reaction. Fluorescence units of control reactions without dNTPs were subtracted from the values obtained in order to give normalized fluorescence units (NFU). ( e ) The chemical structure of C8-alkyne-dCTP.

    Article Snippet: C8-alkyne-dCTP and 5-ethynyl-dUTP were purchased from Jena Bioscience or Baseclick.

    Techniques: Incubation, Labeling, Fluorescence