Structured Review

Boehringer Mannheim taq dna polymerase
(A) Agarose (2%) gel containing RT-PCR samples from heat-shocked and control cells. Lanes 1 and 2 contained control and heat-shocked RNA, respectively. Lanes 3 and 4 contained the RT products in lanes 1 and 2, respectively. Lane 5 contained the products resulting from PCR amplification of the material in lane 3. Lanes 6 and 7 contained parallel PCR mixtures from lane 4. Lanes c4 through c7 contained controls. Lane c4 contained the product resulting from PCR amplification of purified RNA (lanes 1 and 2). Lane c5 contained the product resulting from a complete RT-PCR performed with no initial template RNA. Lane c6 contained the RT-PCR product obtained without AMVRT, and lane c7 contained the PCR product resulting from RNA obtained without <t>DNA</t> <t>Taq</t> polymerase. (B) Agarose (2%) gel containing new RNA samples loaded in the same manner as those in panel A. However, the purified RNA samples were contaminated with DNA, as shown by the multiple bands in control lanes c4 and c6.
Taq Dna Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Price from $9.99 to $1999.99
taq dna polymerase - by Bioz Stars, 2020-12
92/100 stars

Images

1) Product Images from "Reverse Transcription-PCR Differential Display Analysis of Escherichia coli Global Gene Regulation in Response to Heat Shock"

Article Title: Reverse Transcription-PCR Differential Display Analysis of Escherichia coli Global Gene Regulation in Response to Heat Shock

Journal: Applied and Environmental Microbiology

doi:

(A) Agarose (2%) gel containing RT-PCR samples from heat-shocked and control cells. Lanes 1 and 2 contained control and heat-shocked RNA, respectively. Lanes 3 and 4 contained the RT products in lanes 1 and 2, respectively. Lane 5 contained the products resulting from PCR amplification of the material in lane 3. Lanes 6 and 7 contained parallel PCR mixtures from lane 4. Lanes c4 through c7 contained controls. Lane c4 contained the product resulting from PCR amplification of purified RNA (lanes 1 and 2). Lane c5 contained the product resulting from a complete RT-PCR performed with no initial template RNA. Lane c6 contained the RT-PCR product obtained without AMVRT, and lane c7 contained the PCR product resulting from RNA obtained without DNA Taq polymerase. (B) Agarose (2%) gel containing new RNA samples loaded in the same manner as those in panel A. However, the purified RNA samples were contaminated with DNA, as shown by the multiple bands in control lanes c4 and c6.
Figure Legend Snippet: (A) Agarose (2%) gel containing RT-PCR samples from heat-shocked and control cells. Lanes 1 and 2 contained control and heat-shocked RNA, respectively. Lanes 3 and 4 contained the RT products in lanes 1 and 2, respectively. Lane 5 contained the products resulting from PCR amplification of the material in lane 3. Lanes 6 and 7 contained parallel PCR mixtures from lane 4. Lanes c4 through c7 contained controls. Lane c4 contained the product resulting from PCR amplification of purified RNA (lanes 1 and 2). Lane c5 contained the product resulting from a complete RT-PCR performed with no initial template RNA. Lane c6 contained the RT-PCR product obtained without AMVRT, and lane c7 contained the PCR product resulting from RNA obtained without DNA Taq polymerase. (B) Agarose (2%) gel containing new RNA samples loaded in the same manner as those in panel A. However, the purified RNA samples were contaminated with DNA, as shown by the multiple bands in control lanes c4 and c6.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Purification

Related Articles

Clone Assay:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. From a cloned dimeric wild-type HBV genome, 900 and 90 molecules were amplified with Taq polymerase and Taq-Pwo polymerase mixture, respectively. .. An aliquot of the PCR products was cloned, and 8 Taq polymerase-amplified and 17 Taq-Pwo polymerase-amplified HBV genomes were randomly selected for functional analysis.

Amplification:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. In contrast, reactions with Taq polymerase and Taq-Pwo polymerases efficiently amplified as few as 102 and 10 molecules, respectively (Fig. A). .. To confirm these enzyme type-dependent differences in sensitivity, a PCR kinetic was performed with approximately 105 to 106 HBV virion DNA molecules as a template.

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. From a cloned dimeric wild-type HBV genome, 900 and 90 molecules were amplified with Taq polymerase and Taq-Pwo polymerase mixture, respectively. .. An aliquot of the PCR products was cloned, and 8 Taq polymerase-amplified and 17 Taq-Pwo polymerase-amplified HBV genomes were randomly selected for functional analysis.

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Concentration Assay:

Article Title: Reverse Transcription-PCR Differential Display Analysis of Escherichia coli Global Gene Regulation in Response to Heat Shock
Article Snippet: .. Each reaction mixture contained Taq DNA polymerase reaction buffer (10 mM Tris HCl [pH 8.3], 50 mM KCl, 1.5 mM MgCl2 )(Boehringer Mannheim), 0.5 mM dATP, 0.5 mM dCTP, 0.5 mM dTTP, 0.5 mM dGTP (all deoxynucleoside triphosphates were obtained from Boehringer Mannheim), each RT primer (Life Technologies) at a concentration of 0.5 μM, each PCR primer (Life Technologies) at a concentration of 0.5 μM, 0.05 U of Taq DNA polymerase (Boehringer Mannheim) per μl, 5 μl of cDNA, and enough autoclaved deionized water to bring the total volume to 60 μl. .. The PCR cycle consisted of initial denaturation at 94°C for 4 min, followed by 40 cycles consisting of denaturation at 94°C for 1 min, annealing at 40°C for 1 min, and extension at 72°C for 1.5 min. A final extension step consisting of 72°C for 5 min was included.

other:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: The combination of Pwo polymerase with Taq polymerase improved the fidelity of polymerization twofold, which is close to the threefold increase previously determined with a lacI -based assay ( ).

Activity Assay:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Polymerase Chain Reaction:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. Therefore, we calculated the error rate of Taq polymerase and Taq-Pwo polymerase mixture in the 40-cycle full-length PCR. .. Based on the number of initial template molecules, the number of final reaction products, and the number of mutations found per number of bases sequenced, the error rate of Taq polymerase was determined to be 12.1 × 10−5 misincorporations per polymerized nucleotide and that of the Taq-Pwo polymerase mixture was determined to be 6.0 × 10−5 (Table ).

Article Title: Staphylococcus schleiferi subsp. schleiferi Expresses a Fibronectin-Binding Protein
Article Snippet: .. PCR amplifications were performed in a DNA thermal cycler (Perkin-Elmer Cetus) with Taq polymerase (Boehringer Mannheim). .. The PCR mixtures contained 100 pmol of forward and reverse primers, 100 ng of genomic DNA, 200 μM deoxynucleoside triphosphate, reaction buffer (1×), 1.5 mM MgSO4 , and 2.5 U of Taq polymerase in a 100-μl volume.

Article Title: Reverse Transcription-PCR Differential Display Analysis of Escherichia coli Global Gene Regulation in Response to Heat Shock
Article Snippet: .. Each reaction mixture contained Taq DNA polymerase reaction buffer (10 mM Tris HCl [pH 8.3], 50 mM KCl, 1.5 mM MgCl2 )(Boehringer Mannheim), 0.5 mM dATP, 0.5 mM dCTP, 0.5 mM dTTP, 0.5 mM dGTP (all deoxynucleoside triphosphates were obtained from Boehringer Mannheim), each RT primer (Life Technologies) at a concentration of 0.5 μM, each PCR primer (Life Technologies) at a concentration of 0.5 μM, 0.05 U of Taq DNA polymerase (Boehringer Mannheim) per μl, 5 μl of cDNA, and enough autoclaved deionized water to bring the total volume to 60 μl. .. The PCR cycle consisted of initial denaturation at 94°C for 4 min, followed by 40 cycles consisting of denaturation at 94°C for 1 min, annealing at 40°C for 1 min, and extension at 72°C for 1.5 min. A final extension step consisting of 72°C for 5 min was included.

Article Title: Human Macrophage-derived Chemokine (MDC), a Novel Chemoattractant for Monocytes, Monocyte-derived Dendritic Cells, and Natural Killer Cells
Article Snippet: .. The PCR mix contained 0.2 μg of pMP390 plasmid DNA, 1.5 mM MgCl2 , 50 mM KCl, 10 mM Tris, pH 8.4, 0.2 mM each dNTP, 10 μg/ml each primer, and 0.5 μl Taq polymerase (5 U/μl) ( Boehringer Mannheim ). .. The PCR fragment was cloned into the expression vector pDC1, a derivative of pRc/CMV in which the neomycin phosphotransferase gene had been replaced by the mouse dihydrofolate reductase gene from the vector pSV2-dhfr (vector #37146; American Type Culture Collection, Rockville, MD).

Plasmid Preparation:

Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations
Article Snippet: .. To determine which PCR system can be used for amplification of HBV genomes in sera from patients with low levels of viremia, defined copy numbers of plasmid-integrated HBV genomes were amplified with Pwo polymerase, which possesses proofreading activity , Taq polymerase, which lacks proofreading activity , and a Taq-Pwo polymerase mixture. .. The Pwo polymerase did not amplify less than 105 template molecules to a level detectable in an ethidium bromide-stained gel (Fig. A).

Article Title: Human Macrophage-derived Chemokine (MDC), a Novel Chemoattractant for Monocytes, Monocyte-derived Dendritic Cells, and Natural Killer Cells
Article Snippet: .. The PCR mix contained 0.2 μg of pMP390 plasmid DNA, 1.5 mM MgCl2 , 50 mM KCl, 10 mM Tris, pH 8.4, 0.2 mM each dNTP, 10 μg/ml each primer, and 0.5 μl Taq polymerase (5 U/μl) ( Boehringer Mannheim ). .. The PCR fragment was cloned into the expression vector pDC1, a derivative of pRc/CMV in which the neomycin phosphotransferase gene had been replaced by the mouse dihydrofolate reductase gene from the vector pSV2-dhfr (vector #37146; American Type Culture Collection, Rockville, MD).

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  • 93
    Boehringer Mannheim taq polymerase
    ) (plasmid pSM2) with <t>Taq</t> polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with <t>Taq-Pwo</t> ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.
    Taq Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq polymerase/product/Boehringer Mannheim
    Average 93 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2020-12
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    80
    Boehringer Mannheim taq dna polymerase components
    Effect on product amplification of treating <t>Taq</t> <t>DNA</t> polymerase with decreasing amounts of Sau 3A1. Positive reactions contained 20 ng of Escherichia coli NTCC 11151 DNA. Negative reaction mixtures contained no added template. (A) First round of amplification with primers 16F and 16R. Lanes 1, 3, 5, 7, 9, 11, and 13 were positive reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lanes 2, 4, 6, 8, 10, 12, and 14 were negative reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 15 contained the molecular size marker (GIBCO, Paisley, Scotland). (B) Second round of amplification with primers NF and NR. Lanes 1 to 7 contained 1 μl of the negative reaction mixtures from round 1 that had been treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 8 contained the positive control for the amplification, and lane 9 contained the reagent control. Lane 10 contained the molecular size marker (GIBCO).
    Taq Dna Polymerase Components, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase components/product/Boehringer Mannheim
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase components - by Bioz Stars, 2020-12
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      Buy from Supplier

    Image Search Results


    ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

    Journal: Journal of Clinical Microbiology

    Article Title: Amplification of Full-Length Hepatitis B Virus Genomes from Samples from Patients with Low Levels of Viremia: Frequency and Functional Consequences of PCR-Introduced Mutations

    doi:

    Figure Lengend Snippet: ) (plasmid pSM2) with Taq polymerase. (B) Analysis of cloned genomes amplified from 90 template molecules of wild-type HBV DNA with Taq-Pwo ) (plasmid pHBV-SapI); ⊘, mock transfection; M, marker lane with 3.2-kb double-stranded (ds) and single-stranded (ss) HBV DNA. Note that the bands at the 3.2-kb position seen in all lanes of the amplified genomes represent input rather than progeny HBV DNA.

    Article Snippet: Therefore, we calculated the error rate of Taq polymerase and Taq-Pwo polymerase mixture in the 40-cycle full-length PCR.

    Techniques: Plasmid Preparation, Clone Assay, Amplification, Transfection, Marker

    Effect on product amplification of treating Taq DNA polymerase with decreasing amounts of Sau 3A1. Positive reactions contained 20 ng of Escherichia coli NTCC 11151 DNA. Negative reaction mixtures contained no added template. (A) First round of amplification with primers 16F and 16R. Lanes 1, 3, 5, 7, 9, 11, and 13 were positive reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lanes 2, 4, 6, 8, 10, 12, and 14 were negative reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 15 contained the molecular size marker (GIBCO, Paisley, Scotland). (B) Second round of amplification with primers NF and NR. Lanes 1 to 7 contained 1 μl of the negative reaction mixtures from round 1 that had been treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 8 contained the positive control for the amplification, and lane 9 contained the reagent control. Lane 10 contained the molecular size marker (GIBCO).

    Journal: Journal of Clinical Microbiology

    Article Title: Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion

    doi:

    Figure Lengend Snippet: Effect on product amplification of treating Taq DNA polymerase with decreasing amounts of Sau 3A1. Positive reactions contained 20 ng of Escherichia coli NTCC 11151 DNA. Negative reaction mixtures contained no added template. (A) First round of amplification with primers 16F and 16R. Lanes 1, 3, 5, 7, 9, 11, and 13 were positive reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lanes 2, 4, 6, 8, 10, 12, and 14 were negative reaction mixtures treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 15 contained the molecular size marker (GIBCO, Paisley, Scotland). (B) Second round of amplification with primers NF and NR. Lanes 1 to 7 contained 1 μl of the negative reaction mixtures from round 1 that had been treated with 4, 2, 1, 0.5, 0.25, 0.13, and 0 U of Sau 3AI, respectively. Lane 8 contained the positive control for the amplification, and lane 9 contained the reagent control. Lane 10 contained the molecular size marker (GIBCO).

    Article Snippet: Prior to PCR amplification, the water, buffer, MgCl2 and Taq DNA polymerase components were mixed and incubated for 30 min at 37°C with 1.0 U of Sau 3AI (Boehringer Mannheim) per U of Taq DNA polymerase.

    Techniques: Amplification, Marker, Positive Control

    Nested PCRs using primer pairs 16F plus 16R and NR plus NF amplify a product in the absence of an added template. Lanes 1 to 5 were amplified by using Amplitaq LD (Perkin-Elmer, Cheshire, United Kingdom), lanes 6 to 10 were amplified with Amplitaq (Perkin-Elmer), and lanes 11 to 15 were amplified with Taq DNA polymerase (Stratagene, Amsterdam, The Netherlands). Lanes 1, 6, and 11 were positive controls for the outer PCRs; lanes 2, 7, and 12 were reagent controls for the outer reaction; lanes 3, 8, and 13 were positive controls for the nested reaction; lanes 4, 9, and 14 contained 1 μl of the first-round reagent control amplified with the nested primers; and lanes 5, 10, and 15 were reagent controls for the nested PCR. Lane 16 contained the molecular size marker (Promega, Wis.).

    Journal: Journal of Clinical Microbiology

    Article Title: Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion

    doi:

    Figure Lengend Snippet: Nested PCRs using primer pairs 16F plus 16R and NR plus NF amplify a product in the absence of an added template. Lanes 1 to 5 were amplified by using Amplitaq LD (Perkin-Elmer, Cheshire, United Kingdom), lanes 6 to 10 were amplified with Amplitaq (Perkin-Elmer), and lanes 11 to 15 were amplified with Taq DNA polymerase (Stratagene, Amsterdam, The Netherlands). Lanes 1, 6, and 11 were positive controls for the outer PCRs; lanes 2, 7, and 12 were reagent controls for the outer reaction; lanes 3, 8, and 13 were positive controls for the nested reaction; lanes 4, 9, and 14 contained 1 μl of the first-round reagent control amplified with the nested primers; and lanes 5, 10, and 15 were reagent controls for the nested PCR. Lane 16 contained the molecular size marker (Promega, Wis.).

    Article Snippet: Prior to PCR amplification, the water, buffer, MgCl2 and Taq DNA polymerase components were mixed and incubated for 30 min at 37°C with 1.0 U of Sau 3AI (Boehringer Mannheim) per U of Taq DNA polymerase.

    Techniques: Amplification, Nested PCR, Marker