taq dna polymerase with thermopol buffer  (New England Biolabs)


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    New England Biolabs taq dna polymerase with thermopol buffer
    Taq Dna Polymerase With Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase with thermopol buffer/product/New England Biolabs
    Average 94 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase with thermopol buffer - by Bioz Stars, 2020-04
    94/100 stars

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    New England Biolabs taq dna polymerase
    Primer-dimers and UNG degradation products can inhibit PCR amplification . a) The negative control reactions (water and PCR reagents, but no <t>DNA)</t> from the mouse mitochondrial DNA PCR and the PSMA transgene PCR were diluted from 10 -2 to 10 -5 . Both sets of dilutions were used to contaminate a PCR for the PSMA transgene. All reactions were amplified with the ABI Gene Expression Master-mix (containing UNG). b) The same experiment was conducted except the NEB <t>Taq</t> Polymerase and buffer was used (no UNG in the PCR for both generation of the primer-dimers and subsequent PCR). NEB Taq is less efficient than ABI Taq at amplifying the target for the lower band in the PSMA transgene PCR, however inhibition by contaminating primer-dimers is the same regardless of PCR mix. c) The PSMA transgene PCR product and negative control were amplified with the ABI Gene Expression Master-mix and gel purified to remove primer-dimers. These products as well as non-purified PSMA transgene PCR product and negative control (that contain primer-dimers) were used to contaminate a subsequent PSMA transgene PCR. The results indicate that amplification of legitimate PCR target is significantly inhibited by contamination with previously generated negative control reactions using the same primers, regardless of the presence or absence of UNG. It also indicates that the UNG degradation products can weakly inhibit amplification and the UNG degradation products and primer-dimers can completely inhibit PCR amplification.
    Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/New England Biolabs
    Average 99 stars, based on 505 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs pcr
    Primer-dimers and UNG degradation products can inhibit PCR amplification . a) The negative control reactions (water and PCR reagents, but no <t>DNA)</t> from the mouse mitochondrial DNA PCR and the PSMA transgene PCR were diluted from 10 -2 to 10 -5 . Both sets of dilutions were used to contaminate a PCR for the PSMA transgene. All reactions were amplified with the ABI Gene Expression Master-mix (containing UNG). b) The same experiment was conducted except the NEB <t>Taq</t> Polymerase and buffer was used (no UNG in the PCR for both generation of the primer-dimers and subsequent PCR). NEB Taq is less efficient than ABI Taq at amplifying the target for the lower band in the PSMA transgene PCR, however inhibition by contaminating primer-dimers is the same regardless of PCR mix. c) The PSMA transgene PCR product and negative control were amplified with the ABI Gene Expression Master-mix and gel purified to remove primer-dimers. These products as well as non-purified PSMA transgene PCR product and negative control (that contain primer-dimers) were used to contaminate a subsequent PSMA transgene PCR. The results indicate that amplification of legitimate PCR target is significantly inhibited by contamination with previously generated negative control reactions using the same primers, regardless of the presence or absence of UNG. It also indicates that the UNG degradation products can weakly inhibit amplification and the UNG degradation products and primer-dimers can completely inhibit PCR amplification.
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 12229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/New England Biolabs
    Average 99 stars, based on 12229 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Primer-dimers and UNG degradation products can inhibit PCR amplification . a) The negative control reactions (water and PCR reagents, but no DNA) from the mouse mitochondrial DNA PCR and the PSMA transgene PCR were diluted from 10 -2 to 10 -5 . Both sets of dilutions were used to contaminate a PCR for the PSMA transgene. All reactions were amplified with the ABI Gene Expression Master-mix (containing UNG). b) The same experiment was conducted except the NEB Taq Polymerase and buffer was used (no UNG in the PCR for both generation of the primer-dimers and subsequent PCR). NEB Taq is less efficient than ABI Taq at amplifying the target for the lower band in the PSMA transgene PCR, however inhibition by contaminating primer-dimers is the same regardless of PCR mix. c) The PSMA transgene PCR product and negative control were amplified with the ABI Gene Expression Master-mix and gel purified to remove primer-dimers. These products as well as non-purified PSMA transgene PCR product and negative control (that contain primer-dimers) were used to contaminate a subsequent PSMA transgene PCR. The results indicate that amplification of legitimate PCR target is significantly inhibited by contamination with previously generated negative control reactions using the same primers, regardless of the presence or absence of UNG. It also indicates that the UNG degradation products can weakly inhibit amplification and the UNG degradation products and primer-dimers can completely inhibit PCR amplification.

    Journal: BMC Research Notes

    Article Title: False negative results from using common PCR reagents

    doi: 10.1186/1756-0500-4-457

    Figure Lengend Snippet: Primer-dimers and UNG degradation products can inhibit PCR amplification . a) The negative control reactions (water and PCR reagents, but no DNA) from the mouse mitochondrial DNA PCR and the PSMA transgene PCR were diluted from 10 -2 to 10 -5 . Both sets of dilutions were used to contaminate a PCR for the PSMA transgene. All reactions were amplified with the ABI Gene Expression Master-mix (containing UNG). b) The same experiment was conducted except the NEB Taq Polymerase and buffer was used (no UNG in the PCR for both generation of the primer-dimers and subsequent PCR). NEB Taq is less efficient than ABI Taq at amplifying the target for the lower band in the PSMA transgene PCR, however inhibition by contaminating primer-dimers is the same regardless of PCR mix. c) The PSMA transgene PCR product and negative control were amplified with the ABI Gene Expression Master-mix and gel purified to remove primer-dimers. These products as well as non-purified PSMA transgene PCR product and negative control (that contain primer-dimers) were used to contaminate a subsequent PSMA transgene PCR. The results indicate that amplification of legitimate PCR target is significantly inhibited by contamination with previously generated negative control reactions using the same primers, regardless of the presence or absence of UNG. It also indicates that the UNG degradation products can weakly inhibit amplification and the UNG degradation products and primer-dimers can completely inhibit PCR amplification.

    Article Snippet: Sixty nanograms of transgenic mouse DNA was amplified in 25 μL reaction containing 0.5 U Taq DNA Polymerase, 10× ThermoPol Reaction Buffer (New England BioLabs, Inc.), 0.4 mM dNTP (dATP, dTTP, dCTP, and dGTP), 1 μM S49, 1 μM INTA, 1 μM S1368, and 1 μM AS2015.

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control, Expressing, Inhibition, Purification, Generated

    Primer-dimers from previously generated reactions demonstrate a strong inhibitory effect on legitimate target amplification of MLV Gag region . a) The negative control reactions (water and PCR reagents but no DNA) from the PSMA PCR and MLV Gag PCR were diluted as shown. Both sets of dilutions were used to contaminate a PCR for MLV Gag. The MLV Gag PCR used Titanium Taq Polymerase and buffer and the PSMA PCR used NEB Taq Polymerase and buffer, with both PCRs being non-UNG containing. b) A negative control reaction (water and PCR reagents but no DNA) from the MLV Gag PCR was diluted 1 × 10 -7 . This PCR, which used non-UNG containing Titanium Taq Polymerase and buffer, was used to contaminate a subsequent MLV Gag PCR. The MLV-plasmid was used in decreasing copy number, as indicated.

    Journal: BMC Research Notes

    Article Title: False negative results from using common PCR reagents

    doi: 10.1186/1756-0500-4-457

    Figure Lengend Snippet: Primer-dimers from previously generated reactions demonstrate a strong inhibitory effect on legitimate target amplification of MLV Gag region . a) The negative control reactions (water and PCR reagents but no DNA) from the PSMA PCR and MLV Gag PCR were diluted as shown. Both sets of dilutions were used to contaminate a PCR for MLV Gag. The MLV Gag PCR used Titanium Taq Polymerase and buffer and the PSMA PCR used NEB Taq Polymerase and buffer, with both PCRs being non-UNG containing. b) A negative control reaction (water and PCR reagents but no DNA) from the MLV Gag PCR was diluted 1 × 10 -7 . This PCR, which used non-UNG containing Titanium Taq Polymerase and buffer, was used to contaminate a subsequent MLV Gag PCR. The MLV-plasmid was used in decreasing copy number, as indicated.

    Article Snippet: Sixty nanograms of transgenic mouse DNA was amplified in 25 μL reaction containing 0.5 U Taq DNA Polymerase, 10× ThermoPol Reaction Buffer (New England BioLabs, Inc.), 0.4 mM dNTP (dATP, dTTP, dCTP, and dGTP), 1 μM S49, 1 μM INTA, 1 μM S1368, and 1 μM AS2015.

    Techniques: Generated, Amplification, Negative Control, Polymerase Chain Reaction, Plasmid Preparation