taq dna ligase buffer  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Taq DNA Ligase
    Description:
    Taq DNA Ligase 10 000 units
    Catalog Number:
    m0208l
    Price:
    316
    Size:
    10 000 units
    Category:
    DNA Ligases
    Buy from Supplier


    Structured Review

    New England Biolabs taq dna ligase buffer
    Taq DNA Ligase
    Taq DNA Ligase 10 000 units
    https://www.bioz.com/result/taq dna ligase buffer/product/New England Biolabs
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase buffer - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage"

    Article Title: Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gni058

    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).
    Figure Legend Snippet: Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Techniques Used: Functional Assay, Clone Assay, Activity Assay, Amplification, Incubation

    2) Product Images from "SNPWaveTM: a flexible multiplexed SNP genotyping technology"

    Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gnh045

    Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only
    Figure Legend Snippet: Principle of the SNPWave method. Allele-specific ligation probes are hybridized to denatured genomic DNA. SNP allele discrimination is based on the specificity of the Taq ( Thermus aquaticus ) DNA ligase. ( A and B ) Closed circular probes are formed only

    Techniques Used: Ligation

    Related Articles

    Transduction:

    Article Title: RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis
    Article Snippet: The amplified product was transformed into competent cells of PY79 and then transferred to the 3610 background using SPP1-mediated generalized transduction. .. An assembly master mixture was made by combining prepared 5× isothermal assembly reaction buffer (131 mM Tris-HCl, 13.1 mM MgCl2 , 13.1 mM DTT, 8.21 mM PEG-8000, 1.32 mM NAD and 0.26 mM each dNTP) with Phusion DNA polymerase (New England BioLabs) (0.033 units µl−1 ), T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units µl−1 ), Taq DNA ligase (New England BioLabs) (5328 units µl−1 ) and additional dNTPs (267 µM).

    Clone Assay:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs). .. TetR clones (indicative for the presence of pSB890Y) were selected and the presence of the inserts comprising the up- and downstream regions of the target genes was verified by PCR with primers pSB890_seq_f and pSB890_seq_r.

    Article Title: Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose
    Article Snippet: The three amplicons were ligated to pUC19, which was digested with Sal I (Fermentas, China) according to the method of Gibson assembly that included T5 exonuclease (Epicentre, USA), Phusion DNA polymerase (New England Biolabs, USA), and Taq DNA ligase (New England Biolabs, USA) [ ]. .. Moreover, gltX , hemA , and hemL were cloned from the genome of C. glutamicum ATCC 13032 using the primers cggltx-F, cggltx-R and cghema-F, cgdhema-R, and cgdheml-F, cgheml-R. To enhance the stability of HemA, hemA was cloned from the S. arizona genome, which encodes the same predicted HemA amino acid sequence of S. typhimurium .

    Amplification:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: Also the flanking regions ∼1000 bp up- and downstream of the target genes were amplified by PCR using the primer pairs listed in the category “Generation of deletion mutants” in Table S2. .. The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs).

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: .. Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O. ..

    Article Title: Association among Polymorphisms in EGFR Gene Exons, Lifestyle and Risk of Gastric Cancer with Gender Differences in Chinese Han Subjects
    Article Snippet: The protocol for rs28384375 amplification consisted of an initial denaturation for 15 min at 95°C, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 56°C for 1 min, and extension at 72°C for 1 min, followed by a final extension at 72°C for 7 min. .. The ligation reaction for each subject was carried out in a final volume of 10 µl containing 1 × NEB Taq DNA ligase buffer, 12.5 pmol of each probe mix, 0.05 µl Taq DNA ligase [NEB Biotechnology (Beijing)], and 1 µl of multi-PCR product.

    Article Title: RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis
    Article Snippet: Insertions were verified by PCR amplification using primers 3318/3321. .. An assembly master mixture was made by combining prepared 5× isothermal assembly reaction buffer (131 mM Tris-HCl, 13.1 mM MgCl2 , 13.1 mM DTT, 8.21 mM PEG-8000, 1.32 mM NAD and 0.26 mM each dNTP) with Phusion DNA polymerase (New England BioLabs) (0.033 units µl−1 ), T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units µl−1 ), Taq DNA ligase (New England BioLabs) (5328 units µl−1 ) and additional dNTPs (267 µM).

    Article Title: Association of RAGE gene four single nucleotide polymorphisms with the risk, invasion, metastasis and overall survival of gastric cancer in Chinese
    Article Snippet: The steps were as follows: initial denaturation step at 94℃ for 3 min, amplification was carried out by 35 cycles at 94℃ for 15 s, 54℃ for 15 s, and at 72℃ for 30 s, followed by a final elongation cycle at 72℃ for 3 min. .. The connection reaction was performed at adding the PCR product 3 μl, 1 U 10×Taq DNA ligase buffer (New England Biolabs, United States), 0.125 μl Taq DNA ligase (40 U/μl, New England Biolabs, United States ), probe (10p) 0.01 μl/strip and with H2 O added to 10 μl under the condition of by cycles at 94℃ for 30 s, 56℃ for 3 min. LDR fluorescent products were analyzed by ABI 377 sequence analyzer (Perkin-Elmer, Foster City, CA, USA), and the results were analyzed by the fragment analysis software GeneMapper 3.7 (Applied Biosystems, Foster city, CA, USA).

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: The SAT1 marker, amplified from yEP352-SAT1 plasmid using the primers HE4_F and HE4_R, was assembled with the SalI-digested yEPGAP-GFP and yEPGAP-YFP via Gibson assembly to create plasmid yEPGAP-GFP-SAT1 and yEPGAP-YFP-SAT1, respectively. .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour.

    Article Title: Detection of Nucleic Acid Targets Using Ramified Rolling Circle DNA Amplification: A Single Nucleotide Polymorphism Assay Model
    Article Snippet: Ligation mix: 1X NEB DNA Ligase Buffer, 10 Units/ml Taq DNA Ligase (NEB). .. RAM amplification buffer: 45 mM Tris Acetate pH 8.3, 80 mM potassium acetate, 2 mM MgCl2 , 0.1% Triton X-100, 0.001% Antifoam SE-15 (Sigma, St Louis, MO, catalog number A8582), 0.13 U/µl Bst DNA Polymerase, large fragment (NEB), 200 µM dNTPs, 0.12X SYBR-Green (Molecular Probes, supplied as 10,000X stock), 1 nM fluorescein isothiocyanate (FITC, Bio-Rad, Hercules, CA), 10% dimethylsulfoxide (DMSO).

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: Paragraph title: Ligation-mediated amplification ... A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes.

    Article Title: Polymorphism in the Vesicular Monoamine Transporter 2 Gene Decreases the Risk of Parkinson's Disease in Han Chinese Men
    Article Snippet: After amplification, 1 μ L of proteinase K (20 mg/mL) was added and the reactions were heated at 70°C for 10 min and then quenched at 94°C for 15 min. .. The ligation reaction for each subject was carried out in a final volume of 20 μ L containing 20 mM Tris-HCl (pH 7.6), 25 mM potassium acetate, 10 mM magnesium acetate, 10 mM DTT, 1 mM NAD, 0.1% Triton X-100, 10 μ L of multiplex PCR product, 1 pmol of each discriminating oligo, 1 pmol of each common oligo, and 0.5 μ L of 40 U/μ L Taq DNA ligase (New England Biolabs, USA).

    Article Title: Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose
    Article Snippet: The gltX , hemA , and hemL genes were amplified from the wild-type C. glutamicum ATCC 13032 genome using primers gltx-F, gltx-R and hema-F, hema-R, and heml-F, and heml-R, respectively. .. The three amplicons were ligated to pUC19, which was digested with Sal I (Fermentas, China) according to the method of Gibson assembly that included T5 exonuclease (Epicentre, USA), Phusion DNA polymerase (New England Biolabs, USA), and Taq DNA ligase (New England Biolabs, USA) [ ].

    Blocking Assay:

    Article Title: biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
    Article Snippet: .. The DNA fragments, 4 µL 5× IRB [25% (wt/vol) PEG-8000, 500 mM Tris∙HCl pH 7.5, 50 mM MgCl2 , 50 mM DTT, 1 mM each of the four dNTPs, 5 mM NAD] , 2 µL Taq DNA Ligase (M0208, New England Biolabs), 0.25 µL Phusion HF DNA Polymerase (F-530, Thermo Scientific), and 0.25 µL T5 Exonuclease (prediluted 1:30 in 1× IRB) (T5E4111K, Epicentre) were mixed on ice in a total volume of 20 µL and immediately transferred to a 50 °C preheated thermocycler block and incubated for 60 min. Additional details can be found in . .. Random DNA sequences of defined base composition were generated using the FaBox online toolbox ( ).

    SYBR Green Assay:

    Article Title: Detection of Nucleic Acid Targets Using Ramified Rolling Circle DNA Amplification: A Single Nucleotide Polymorphism Assay Model
    Article Snippet: Ligation mix: 1X NEB DNA Ligase Buffer, 10 Units/ml Taq DNA Ligase (NEB). .. RAM amplification buffer: 45 mM Tris Acetate pH 8.3, 80 mM potassium acetate, 2 mM MgCl2 , 0.1% Triton X-100, 0.001% Antifoam SE-15 (Sigma, St Louis, MO, catalog number A8582), 0.13 U/µl Bst DNA Polymerase, large fragment (NEB), 200 µM dNTPs, 0.12X SYBR-Green (Molecular Probes, supplied as 10,000X stock), 1 nM fluorescein isothiocyanate (FITC, Bio-Rad, Hercules, CA), 10% dimethylsulfoxide (DMSO).

    Incubation:

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour. .. The 5′ and 3′ flanking regions for the fluorescent integration cassettes were amplified from genomic DNA (gDNA) of ATCC 2001 using the primer pairs HE6_F and HE6_R and HE7_F and HE7_R respectively, which contain homologous sequences to the pseudo gene CAGL0C01067g.

    Article Title: biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
    Article Snippet: .. The DNA fragments, 4 µL 5× IRB [25% (wt/vol) PEG-8000, 500 mM Tris∙HCl pH 7.5, 50 mM MgCl2 , 50 mM DTT, 1 mM each of the four dNTPs, 5 mM NAD] , 2 µL Taq DNA Ligase (M0208, New England Biolabs), 0.25 µL Phusion HF DNA Polymerase (F-530, Thermo Scientific), and 0.25 µL T5 Exonuclease (prediluted 1:30 in 1× IRB) (T5E4111K, Epicentre) were mixed on ice in a total volume of 20 µL and immediately transferred to a 50 °C preheated thermocycler block and incubated for 60 min. Additional details can be found in . .. Random DNA sequences of defined base composition were generated using the FaBox online toolbox ( ).

    Article Title: Detection of Nucleic Acid Targets Using Ramified Rolling Circle DNA Amplification: A Single Nucleotide Polymorphism Assay Model
    Article Snippet: Incubation for 30 minutes at 37°C was followed by enzyme deactivation at 65°C for 30 minutes. .. Ligation mix: 1X NEB DNA Ligase Buffer, 10 Units/ml Taq DNA Ligase (NEB).

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: .. A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes. .. A volume of 15 μl of a HotStarTaq DNA Polymerase mix (Qiagen, hilden, Germany) containing 16 μmol/l of each dNTP (Invitrogen) and 100 nmol/l of T3 primer and biotinylated T7 primer was added.

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100. .. The hybridization reactions were heated to 95°C for 90 seconds and incubated at 37°C for 40 minutes to allow hybridization between allele-specific LDR products and microsphere-labeled anti-TAG probes.

    Western Blot:

    Article Title: A protective Zika virus E-dimer-based subunit vaccine engineered to abrogate antibody-dependent enhancement of dengue infection
    Article Snippet: Protein G/Protein A agar suspension beads (IP10; 10 ml) and Luminata Classico Western Blot developer solution (WBLUC0500) were obtained from Merck Chemicals. .. Phusion DNA polymerase (M0530) and Taq DNA ligase (M0208) were purchased from New England BioLabs) 5′-T5 exonuclease (T5E4111K) was purchased from Epicentre.

    Transformation Assay:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs). .. Next, the strain E. coli 118λpir was transformed with the resulting plasmid.

    Article Title: RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis
    Article Snippet: The amplified product was transformed into competent cells of PY79 and then transferred to the 3610 background using SPP1-mediated generalized transduction. .. An assembly master mixture was made by combining prepared 5× isothermal assembly reaction buffer (131 mM Tris-HCl, 13.1 mM MgCl2 , 13.1 mM DTT, 8.21 mM PEG-8000, 1.32 mM NAD and 0.26 mM each dNTP) with Phusion DNA polymerase (New England BioLabs) (0.033 units µl−1 ), T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units µl−1 ), Taq DNA ligase (New England BioLabs) (5328 units µl−1 ) and additional dNTPs (267 µM).

    Hybridization:

    Article Title: Detection of Nucleic Acid Targets Using Ramified Rolling Circle DNA Amplification: A Single Nucleotide Polymorphism Assay Model
    Article Snippet: 50 µl of beads resuspended to 0.025% solids in 1X hybridization buffer was added per well of a bead source KF plate. .. Ligation mix: 1X NEB DNA Ligase Buffer, 10 Units/ml Taq DNA Ligase (NEB).

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes. .. Total time from the addition of lysis buffer to hybridization-ready product for 96 samples processed in parallel in a single microtiter plate is approximately 14 hours.

    Article Title: Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays
    Article Snippet: .. Briefly, the ligations were carried out in a final volume of 20 μl containing 1× ligation buffer (TAQ ligase buffer, New England Biolabs, MA, USA), 30 mM tetramethylammonium chloride (TMAC), 250 fmol of each discriminating probe, 250 fmol of each common probe, 5 pmol of the complementary hybridisation control probe, a variable amount of purified PCR products and 4 U of Taq DNA ligase (New England Biolabs). .. The reaction was cycled for 40 rounds at 94°C for 30 s and at 60-64°C for 4 min in a thermocycler (MJ Research).

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100. .. Five microliters of the multiplexed LDR reaction was added to 60 μL of hybridization solution: 3 mol/L tetramethylammonium chloride (TMAC), 50 mmol/L Tris-HCl, pH 8.0, 3 mmol/L EDTA, pH 8.0, and 0.1% sodium dodecyl sulfate, containing 250 Luminex FlexMAP microspheres from each allelic set (total number = 5).

    Electroporation:

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour. .. The fluorescent integration plasmids were then digested with SphI and integrated into the genome of ATCC 2001 by electroporation to generate the fluorescently marked strains MHCg-G and MHCg-Y, respectively.

    Ligation:

    Article Title: Association among Polymorphisms in EGFR Gene Exons, Lifestyle and Risk of Gastric Cancer with Gender Differences in Chinese Han Subjects
    Article Snippet: .. The ligation reaction for each subject was carried out in a final volume of 10 µl containing 1 × NEB Taq DNA ligase buffer, 12.5 pmol of each probe mix, 0.05 µl Taq DNA ligase [NEB Biotechnology (Beijing)], and 1 µl of multi-PCR product. ..

    Article Title: Detection of Nucleic Acid Targets Using Ramified Rolling Circle DNA Amplification: A Single Nucleotide Polymorphism Assay Model
    Article Snippet: .. Ligation mix: 1X NEB DNA Ligase Buffer, 10 Units/ml Taq DNA Ligase (NEB). ..

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: Paragraph title: Ligation-mediated amplification ... A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes.

    Article Title: Polymorphism in the Vesicular Monoamine Transporter 2 Gene Decreases the Risk of Parkinson's Disease in Han Chinese Men
    Article Snippet: .. The ligation reaction for each subject was carried out in a final volume of 20 μ L containing 20 mM Tris-HCl (pH 7.6), 25 mM potassium acetate, 10 mM magnesium acetate, 10 mM DTT, 1 mM NAD, 0.1% Triton X-100, 10 μ L of multiplex PCR product, 1 pmol of each discriminating oligo, 1 pmol of each common oligo, and 0.5 μ L of 40 U/μ L Taq DNA ligase (New England Biolabs, USA). ..

    Article Title: Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays
    Article Snippet: .. Briefly, the ligations were carried out in a final volume of 20 μl containing 1× ligation buffer (TAQ ligase buffer, New England Biolabs, MA, USA), 30 mM tetramethylammonium chloride (TMAC), 250 fmol of each discriminating probe, 250 fmol of each common probe, 5 pmol of the complementary hybridisation control probe, a variable amount of purified PCR products and 4 U of Taq DNA ligase (New England Biolabs). .. The reaction was cycled for 40 rounds at 94°C for 30 s and at 60-64°C for 4 min in a thermocycler (MJ Research).

    Generated:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: Generation of knockout strains The knockout strains, that are listed in Table S1 were generated using the suicide plasmid pSB890Y (a derivative of pSB890 where internal PstI restriction sites were removed). .. The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs).

    DNA Sequencing:

    Article Title: Association among Polymorphisms in EGFR Gene Exons, Lifestyle and Risk of Gastric Cancer with Gender Differences in Chinese Han Subjects
    Article Snippet: The ligation reaction for each subject was carried out in a final volume of 10 µl containing 1 × NEB Taq DNA ligase buffer, 12.5 pmol of each probe mix, 0.05 µl Taq DNA ligase [NEB Biotechnology (Beijing)], and 1 µl of multi-PCR product. .. To confirm the accuracy of the PCR-LDR genotyping method, direct DNA sequencing of randomly selected PCR products was performed.

    Polymerase Chain Reaction:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: Also the flanking regions ∼1000 bp up- and downstream of the target genes were amplified by PCR using the primer pairs listed in the category “Generation of deletion mutants” in Table S2. .. The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs).

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: Reactions were performed on a thermal cycler Gene Amp PCR system 9600 (Perkin Elmer, Waltham, MA, USA). .. Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O.

    Article Title: Association among Polymorphisms in EGFR Gene Exons, Lifestyle and Risk of Gastric Cancer with Gender Differences in Chinese Han Subjects
    Article Snippet: Thermal cycling was performed for five SNPs (rs2227983, rs17337023, rs1140475, rs2293347 and rs2072454) in the Gene Amp PCR system 9600 (PerkinElmer) with an initial denaturation for 15 min at 95°C, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 59°C for 1 min, and extension at 72°C for 1 min, followed by a final extension at 72°C for 7 min. .. The ligation reaction for each subject was carried out in a final volume of 10 µl containing 1 × NEB Taq DNA ligase buffer, 12.5 pmol of each probe mix, 0.05 µl Taq DNA ligase [NEB Biotechnology (Beijing)], and 1 µl of multi-PCR product.

    Article Title: RemA is a DNA-binding protein that activates biofilm matrix gene expression in Bacillus subtilis
    Article Snippet: Insertions were verified by PCR amplification using primers 3318/3321. .. An assembly master mixture was made by combining prepared 5× isothermal assembly reaction buffer (131 mM Tris-HCl, 13.1 mM MgCl2 , 13.1 mM DTT, 8.21 mM PEG-8000, 1.32 mM NAD and 0.26 mM each dNTP) with Phusion DNA polymerase (New England BioLabs) (0.033 units µl−1 ), T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units µl−1 ), Taq DNA ligase (New England BioLabs) (5328 units µl−1 ) and additional dNTPs (267 µM).

    Article Title: Association of RAGE gene four single nucleotide polymorphisms with the risk, invasion, metastasis and overall survival of gastric cancer in Chinese
    Article Snippet: .. The connection reaction was performed at adding the PCR product 3 μl, 1 U 10×Taq DNA ligase buffer (New England Biolabs, United States), 0.125 μl Taq DNA ligase (40 U/μl, New England Biolabs, United States ), probe (10p) 0.01 μl/strip and with H2 O added to 10 μl under the condition of by cycles at 94℃ for 30 s, 56℃ for 3 min. LDR fluorescent products were analyzed by ABI 377 sequence analyzer (Perkin-Elmer, Foster City, CA, USA), and the results were analyzed by the fragment analysis software GeneMapper 3.7 (Applied Biosystems, Foster city, CA, USA). .. To test the accuracy of PCR-LDR method, a total of 96 DNA samples were randomly selected from the total sample, and they were genotyped using the same method, with exactly the sample findings for duplicated samples.

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: The PCR products and the vector yEPGAP-cherry were digested with EcoRI and XhoI. .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour.

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes. .. The plate was covered, spun as before, and polymerase chain reaction performed in a Thermo Electron (Milford, MA, USA) MBS 384 Satellite Thermal Cycler (initial denaturation of 92°C for 9 minutes, 92°C for 30 s, 60°C for 30 s, 72°C for 30 s for 39 cycles; final extension at 72°C for 5 minutes).

    Article Title: Polymorphism in the Vesicular Monoamine Transporter 2 Gene Decreases the Risk of Parkinson's Disease in Han Chinese Men
    Article Snippet: .. The ligation reaction for each subject was carried out in a final volume of 20 μ L containing 20 mM Tris-HCl (pH 7.6), 25 mM potassium acetate, 10 mM magnesium acetate, 10 mM DTT, 1 mM NAD, 0.1% Triton X-100, 10 μ L of multiplex PCR product, 1 pmol of each discriminating oligo, 1 pmol of each common oligo, and 0.5 μ L of 40 U/μ L Taq DNA ligase (New England Biolabs, USA). ..

    Article Title: Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays
    Article Snippet: .. Briefly, the ligations were carried out in a final volume of 20 μl containing 1× ligation buffer (TAQ ligase buffer, New England Biolabs, MA, USA), 30 mM tetramethylammonium chloride (TMAC), 250 fmol of each discriminating probe, 250 fmol of each common probe, 5 pmol of the complementary hybridisation control probe, a variable amount of purified PCR products and 4 U of Taq DNA ligase (New England Biolabs). .. The reaction was cycled for 40 rounds at 94°C for 30 s and at 60-64°C for 4 min in a thermocycler (MJ Research).

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: .. The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100. ..

    Isolation:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs). .. The plasmid was then isolated using the peqGold plasmid miniprep kitI (VWR) and transformed into the diaminopimelic acid auxotroph strain E. coli ß2163.

    Labeling:

    Article Title: Performance of probe polymerization-conjunction-agarose gel electrophoresis in the rapid detection of KRAS gene mutation
    Article Snippet: Next, the tubes were added with 1 μL of deoxyribonucleotide (25 μM) bases that are complementary with the labeling of different tubes (for example, dATP was added to tube T, and dCTP was added to tube G). .. Subsequently, 1 μL of enzyme solution (1 unit Pfu of DNA polymerase (Shanghai Sangon, Co., Ltd., China) and 2 units of Taq DNA ligase (New England BioLabs, USA) were added.

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: Additionally, a Plasmodium -specific fluorescently labeled conserved probe (designated “Common1”) and a Pf specific probe (designated “Pf1”) previously described were included in the LDR-FMA reaction. .. The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100.

    Purification:

    Article Title: Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays
    Article Snippet: .. Briefly, the ligations were carried out in a final volume of 20 μl containing 1× ligation buffer (TAQ ligase buffer, New England Biolabs, MA, USA), 30 mM tetramethylammonium chloride (TMAC), 250 fmol of each discriminating probe, 250 fmol of each common probe, 5 pmol of the complementary hybridisation control probe, a variable amount of purified PCR products and 4 U of Taq DNA ligase (New England Biolabs). .. The reaction was cycled for 40 rounds at 94°C for 30 s and at 60-64°C for 4 min in a thermocycler (MJ Research).

    Sequencing:

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O. .. Reproducibility of genotyping was confirmed by sequencing in 400 randomly selected samples with 100% concordance.

    Article Title: Association of RAGE gene four single nucleotide polymorphisms with the risk, invasion, metastasis and overall survival of gastric cancer in Chinese
    Article Snippet: .. The connection reaction was performed at adding the PCR product 3 μl, 1 U 10×Taq DNA ligase buffer (New England Biolabs, United States), 0.125 μl Taq DNA ligase (40 U/μl, New England Biolabs, United States ), probe (10p) 0.01 μl/strip and with H2 O added to 10 μl under the condition of by cycles at 94℃ for 30 s, 56℃ for 3 min. LDR fluorescent products were analyzed by ABI 377 sequence analyzer (Perkin-Elmer, Foster City, CA, USA), and the results were analyzed by the fragment analysis software GeneMapper 3.7 (Applied Biosystems, Foster city, CA, USA). .. To test the accuracy of PCR-LDR method, a total of 96 DNA samples were randomly selected from the total sample, and they were genotyped using the same method, with exactly the sample findings for duplicated samples.

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: In total, five specific probes (four MSP-119 –allele specific and one Pf-specific) and three conserved sequence probes were included in the multiplexed detection reaction. .. The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100.

    Article Title: Engineering Corynebacterium glutamicum to produce 5-aminolevulinic acid from glucose
    Article Snippet: The three amplicons were ligated to pUC19, which was digested with Sal I (Fermentas, China) according to the method of Gibson assembly that included T5 exonuclease (Epicentre, USA), Phusion DNA polymerase (New England Biolabs, USA), and Taq DNA ligase (New England Biolabs, USA) [ ]. .. Moreover, gltX , hemA , and hemL were cloned from the genome of C. glutamicum ATCC 13032 using the primers cggltx-F, cggltx-R and cghema-F, cgdhema-R, and cgdheml-F, cgheml-R. To enhance the stability of HemA, hemA was cloned from the S. arizona genome, which encodes the same predicted HemA amino acid sequence of S. typhimurium .

    Construct:

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: The fluorescent C . glabrata strains, MHCg-Y and MHCg-G (as KKY and KKG, respectively, previously used in ), were constructed as follows: the gene encoding green fluorescent protein (GFP) was amplified from pGS62 plasmid using primer pair of HE1_F and HE1_R; the gene encoding yellow fluorescent protein (YFP) was amplified from pGS63 using primer pair HE2_F and HE2_R. .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour.

    Plasmid Preparation:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: .. The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs). .. Next, the strain E. coli 118λpir was transformed with the resulting plasmid.

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: The SAT1 marker, amplified from yEP352-SAT1 plasmid using the primers HE4_F and HE4_R, was assembled with the SalI-digested yEPGAP-GFP and yEPGAP-YFP via Gibson assembly to create plasmid yEPGAP-GFP-SAT1 and yEPGAP-YFP-SAT1, respectively. .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour.

    Software:

    Article Title: Association of RAGE gene four single nucleotide polymorphisms with the risk, invasion, metastasis and overall survival of gastric cancer in Chinese
    Article Snippet: .. The connection reaction was performed at adding the PCR product 3 μl, 1 U 10×Taq DNA ligase buffer (New England Biolabs, United States), 0.125 μl Taq DNA ligase (40 U/μl, New England Biolabs, United States ), probe (10p) 0.01 μl/strip and with H2 O added to 10 μl under the condition of by cycles at 94℃ for 30 s, 56℃ for 3 min. LDR fluorescent products were analyzed by ABI 377 sequence analyzer (Perkin-Elmer, Foster City, CA, USA), and the results were analyzed by the fragment analysis software GeneMapper 3.7 (Applied Biosystems, Foster city, CA, USA). .. To test the accuracy of PCR-LDR method, a total of 96 DNA samples were randomly selected from the total sample, and they were genotyped using the same method, with exactly the sample findings for duplicated samples.

    Multiplex Assay:

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: .. Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O. ..

    Article Title: Association among Polymorphisms in EGFR Gene Exons, Lifestyle and Risk of Gastric Cancer with Gender Differences in Chinese Han Subjects
    Article Snippet: The target DNA sequences were amplified using a multiplex PCR method. .. The ligation reaction for each subject was carried out in a final volume of 10 µl containing 1 × NEB Taq DNA ligase buffer, 12.5 pmol of each probe mix, 0.05 µl Taq DNA ligase [NEB Biotechnology (Beijing)], and 1 µl of multi-PCR product.

    Article Title: Polymorphism in the Vesicular Monoamine Transporter 2 Gene Decreases the Risk of Parkinson's Disease in Han Chinese Men
    Article Snippet: .. The ligation reaction for each subject was carried out in a final volume of 20 μ L containing 20 mM Tris-HCl (pH 7.6), 25 mM potassium acetate, 10 mM magnesium acetate, 10 mM DTT, 1 mM NAD, 0.1% Triton X-100, 10 μ L of multiplex PCR product, 1 pmol of each discriminating oligo, 1 pmol of each common oligo, and 0.5 μ L of 40 U/μ L Taq DNA ligase (New England Biolabs, USA). ..

    Selection:

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: Paragraph title: Gene variant selection and genotyping ... Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O.

    Article Title: Association of RAGE gene four single nucleotide polymorphisms with the risk, invasion, metastasis and overall survival of gastric cancer in Chinese
    Article Snippet: Paragraph title: SNP Selection and Genotyping ... The connection reaction was performed at adding the PCR product 3 μl, 1 U 10×Taq DNA ligase buffer (New England Biolabs, United States), 0.125 μl Taq DNA ligase (40 U/μl, New England Biolabs, United States ), probe (10p) 0.01 μl/strip and with H2 O added to 10 μl under the condition of by cycles at 94℃ for 30 s, 56℃ for 3 min. LDR fluorescent products were analyzed by ABI 377 sequence analyzer (Perkin-Elmer, Foster City, CA, USA), and the results were analyzed by the fragment analysis software GeneMapper 3.7 (Applied Biosystems, Foster city, CA, USA).

    Knock-Out:

    Article Title: Identifying components required for OMP biogenesis as novel targets for antiinfective drugs
    Article Snippet: Paragraph title: Generation of knockout strains ... The vector pSB890Y and the flanking regions were then assembled using a selfmade Gibson Assembly Master Mix containing T5 Exonuclease (10U µl, Epicentre), Phusion Polymerase (2U µl, New England Biolabs) and Taq-DNA-Ligase (40U µl, New England Biolabs).

    Marker:

    Article Title: CgSTE11 mediates cross tolerance to multiple environmental stressors in Candida glabrata
    Article Snippet: The SAT1 marker, amplified from yEP352-SAT1 plasmid using the primers HE4_F and HE4_R, was assembled with the SalI-digested yEPGAP-GFP and yEPGAP-YFP via Gibson assembly to create plasmid yEPGAP-GFP-SAT1 and yEPGAP-YFP-SAT1, respectively. .. Gibson assembly reactions were carried out using Phusion® High-Fidelity DNA Polymerase (NEB), Taq DNA ligase (NEB), and T5 exonuclease (NEB); and the reaction was incubated at 50 °C for 1 hour.

    Lysis:

    Article Title: A method for high-throughput gene expression signature analysis
    Article Snippet: Ligation-mediated amplification Transcripts were captured in oligo-dT coated 384 well plates (GenePlateHT; RNAture) from total RNA (500 ng) in Lysis Buffer (RNAture) or whole cell lysates (20 μl). .. A volume of 5 μl of 1× Taq Ligase Buffer containing 2.5 U Taq DNA ligase (New England Biolabs) was added, the plate covered, spun as before, and incubated at 45°C for one hour followed by 65°C for 10 minutes.

    Variant Assay:

    Article Title: The Human Myotrophin Variant Attenuates MicroRNA-Let-7 Binding Ability but Not Risk of Left Ventricular Hypertrophy in Human Essential Hypertension
    Article Snippet: Paragraph title: Gene variant selection and genotyping ... Further amplification was performed in a 10 μl volume of multiplex LDR reaction mixture, containing 1 μl (100 ng) of the resultant probe mix, 1 μl of probe mix, 0.05 μl of NEB Taq DNA ligase and 7.95 μl of ddH2 O.

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: The LDR-FMA was adapted to distinguish between single nucleotide polymorphisms that recognize G versus A corresponding to amino acid position 1644 (E versus Q variant), and GCA versus ACG (position 1699–1701) corresponding to the last two nucleotide bases of S and N codons and the first base of the R and G codons, respectively. .. The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100.

    Luminex:

    Article Title: A Polymerase Chain Reaction/Ligase Detection Reaction-Fluorescent Microsphere Assay to Determine Plasmodium falciparum MSP-119 Haplotypes
    Article Snippet: The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100. .. The LDR reaction was performed with 1 μL of the PCR reaction, 10 nmol/L (200 fmol) of each probe, and 2 units of Taq DNA ligase (New England Biolabs, Beverly, MA) in a buffer containing 20 mmol/L Tris-HCl buffer, pH 7.6, 25 mmol/L potassium acetate, 10 mmol/L magnesium acetate, 1 mmol/L NAD+, 10 mmol/L dithiothreitol, and 0.1% Triton X-100.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs taq dna ligase
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase/product/New England Biolabs
    Average 90 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs taq dna ligase reaction buffer
    Taq Dna Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase reaction buffer/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase reaction buffer - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results