talon superflow metal affinity resin  (TaKaRa)

 
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    Name:
    TALON Superflow Metal Affinity Resin
    Description:
    TALON his tag purification resin lets you prepare exceptionally pure his tagged proteins from bacterial mammalian yeast and baculovirus infected cells under native or denaturing conditions TALON is an immobilized metal affinity chromatography IMAC resin charged with cobalt which binds to his tagged proteins with higher specificity than nickel charged resins As a result TALON resin delivers his tagged proteins of the highest purity In addition each cobalt ion is bound to the resin at four sites resulting in low metal ion leakage
    Catalog Number:
    635670
    Price:
    None
    Size:
    250 mL
    Category:
    Cobalt resin FPLC Resin in FPLC cartridges His tagged protein purification Purification products Protein research
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    Structured Review

    TaKaRa talon superflow metal affinity resin
    TALON his tag purification resin lets you prepare exceptionally pure his tagged proteins from bacterial mammalian yeast and baculovirus infected cells under native or denaturing conditions TALON is an immobilized metal affinity chromatography IMAC resin charged with cobalt which binds to his tagged proteins with higher specificity than nickel charged resins As a result TALON resin delivers his tagged proteins of the highest purity In addition each cobalt ion is bound to the resin at four sites resulting in low metal ion leakage
    https://www.bioz.com/result/talon superflow metal affinity resin/product/TaKaRa
    Average 95 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    talon superflow metal affinity resin - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Affinity Chromatography:

    Article Title: Ethylphenol Formation by Lactobacillus plantarum: Identification of the Enzyme Involved in the Reduction of Vinylphenols
    Article Snippet: .. The filtered supernatant containing VprA protein was analyzed for reductase activity by adding 1.5 mM 4-vinylphenol and incubating at 37°C during 16 h. VprA protein was purified for affinity chromatography in a Talon Superflow resin (Clontech), and the purity of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer. .. Fractions containing the His6 -tagged proteins were pooled and analyzed for vinylphenol reductase activity.

    Article Title: Unravelling the Reduction Pathway as an Alternative Metabolic Route to Hydroxycinnamate Decarboxylation in Lactobacillus plantarum
    Article Snippet: .. The supernatants of the HcrA, HcrB, and HcrAB proteins were analyzed for hydroxycinnamate reductase by adding 1.5 mM m -coumaric acid and incubating at 37°C for 16 h. For purification of the recombinant His6 -tagged proteins, the filtered supernatants were applied to a TALON Superflow resin (Clontech) equilibrated with the buffer described previously containing 0.3 M NaCl and 10 mM imidazole to improve the interaction specificity in the affinity chromatography step. .. The purity of the enzymes was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer.

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: .. Briefly, after 16 h of expression at 16 °C via isopropyl –D-1-thiogalactopyranoside (IPTG) induction in LB medium after cells reached an OD600 of 0.6, cells were harvested, pelleted and purified by affinity chromatography over Talon Superflow resin (Takara Clontech). .. The His6 affinity tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4), 150 mM NaCl.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Ethylphenol Formation by Lactobacillus plantarum: Identification of the Enzyme Involved in the Reduction of Vinylphenols
    Article Snippet: .. The filtered supernatant containing VprA protein was analyzed for reductase activity by adding 1.5 mM 4-vinylphenol and incubating at 37°C during 16 h. VprA protein was purified for affinity chromatography in a Talon Superflow resin (Clontech), and the purity of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer. .. Fractions containing the His6 -tagged proteins were pooled and analyzed for vinylphenol reductase activity.

    Protease Inhibitor:

    Article Title: ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function
    Article Snippet: .. GST fusion proteins as well as HisTrx-tagged proteins were expressed in Escherichia coli (BL21) at 37°C for 4 h according to standard methods, and purified from cell lysates with high-affinity GST matrix (GenScript) in PBS or talon superflow metal affinity matrix (Takara Bio Inc.) in 50 mM sodium phosphate and 300 mM sodium chloride, pH 7.0, respectively, in the presence of protease inhibitor Complete EDTA-free (Roche), 1% (vol/vol) Triton X-100, DNase I, and 10 mM magnesium chloride. ..

    Purification:

    Article Title: Ethylphenol Formation by Lactobacillus plantarum: Identification of the Enzyme Involved in the Reduction of Vinylphenols
    Article Snippet: .. The filtered supernatant containing VprA protein was analyzed for reductase activity by adding 1.5 mM 4-vinylphenol and incubating at 37°C during 16 h. VprA protein was purified for affinity chromatography in a Talon Superflow resin (Clontech), and the purity of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer. .. Fractions containing the His6 -tagged proteins were pooled and analyzed for vinylphenol reductase activity.

    Article Title: Unravelling the Reduction Pathway as an Alternative Metabolic Route to Hydroxycinnamate Decarboxylation in Lactobacillus plantarum
    Article Snippet: .. The supernatants of the HcrA, HcrB, and HcrAB proteins were analyzed for hydroxycinnamate reductase by adding 1.5 mM m -coumaric acid and incubating at 37°C for 16 h. For purification of the recombinant His6 -tagged proteins, the filtered supernatants were applied to a TALON Superflow resin (Clontech) equilibrated with the buffer described previously containing 0.3 M NaCl and 10 mM imidazole to improve the interaction specificity in the affinity chromatography step. .. The purity of the enzymes was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer.

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: .. Briefly, after 16 h of expression at 16 °C via isopropyl –D-1-thiogalactopyranoside (IPTG) induction in LB medium after cells reached an OD600 of 0.6, cells were harvested, pelleted and purified by affinity chromatography over Talon Superflow resin (Takara Clontech). .. The His6 affinity tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4), 150 mM NaCl.

    Article Title: ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function
    Article Snippet: .. GST fusion proteins as well as HisTrx-tagged proteins were expressed in Escherichia coli (BL21) at 37°C for 4 h according to standard methods, and purified from cell lysates with high-affinity GST matrix (GenScript) in PBS or talon superflow metal affinity matrix (Takara Bio Inc.) in 50 mM sodium phosphate and 300 mM sodium chloride, pH 7.0, respectively, in the presence of protease inhibitor Complete EDTA-free (Roche), 1% (vol/vol) Triton X-100, DNase I, and 10 mM magnesium chloride. ..

    Incubation:

    Article Title: Morphologic determinant of tight junctions revealed by claudin-3 structures
    Article Snippet: .. Insoluble material was removed by ultracentrifugation at 100,000 × g for 45 min, and the supernatant was incubated with TALON Superflow cobalt affinity resin (Clontech) for 3 h at 4 °C. ..

    Activity Assay:

    Article Title: Ethylphenol Formation by Lactobacillus plantarum: Identification of the Enzyme Involved in the Reduction of Vinylphenols
    Article Snippet: .. The filtered supernatant containing VprA protein was analyzed for reductase activity by adding 1.5 mM 4-vinylphenol and incubating at 37°C during 16 h. VprA protein was purified for affinity chromatography in a Talon Superflow resin (Clontech), and the purity of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer. .. Fractions containing the His6 -tagged proteins were pooled and analyzed for vinylphenol reductase activity.

    Expressing:

    Article Title: Distinct activation modes of the Relaxin Family Peptide Receptor 2 in response to insulin-like peptide 3 and relaxin
    Article Snippet: .. Briefly, after 16 h of expression at 16 °C via isopropyl –D-1-thiogalactopyranoside (IPTG) induction in LB medium after cells reached an OD600 of 0.6, cells were harvested, pelleted and purified by affinity chromatography over Talon Superflow resin (Takara Clontech). .. The His6 affinity tag was removed by thrombin cleavage and the proteins further purified with a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) in 20 mM Tris HCl (pH 7.4), 150 mM NaCl.

    Western Blot:

    Article Title: Molecular Interface of S100A8 with Cytochrome b558 and NADPH Oxidase Activation
    Article Snippet: .. Materials Chemical reagents used in this study were obtained from the following sources: Heparin agarose, arachidonic acid, phorbol myristate acetate, carbenicillin, luminol (5-amino2, 3-dihydro-1, 4-phtalazinedione), monoclonal antibodies anti polyHistidine (Sigma Chemicals Co., St Louis, MO, USA); S100A8 (Calgranulin A, C-19) goat polyclonal antibody, donkey-anti-goat IgG antibody, S100A12 (Calgranulin C, 19F5) monoclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA); Anti-goat and anti-rabbit IgG Alexa Fluor 488 or 546, or anti-mouse IgG Alexa Fluor 546 or 488 antibodies (Molecular Probes Europe BV Leiden, The Netherlands); PIA (DIFCO Laboratories, Detroit, MI, USA); ECL Western blot detection reagent, Sephacryl S-300 HR, diethylaminoethyl (DEAE) Sepharose CL-6B, CM-Sepharose CL-6B, N-amino octyl-Sepharose CL-4B,protein G Sepharose (Amersham Pharmacia Biotech, Uppsala, Sweden); N-octyl glucoside (Roche diagnostic, Meylan, France); DSS (Disuccinimidyl suberate) (Pierce Chemical Co, USA); Trizol® was from Invitrogen Life Technologies, Grand Island, NY , USA; Talon® SuperflowTM Metal Affinity was from Clonetech, Mountain View, Ca, USA; Nitrocellulose membrane 0.45 µm was from Bio-Rad Laboratories, Hercules, CA, USA. .. Polyclonal antibodies raised against S100 proteins purified from cytosol of neutrophils were described previously .

    Recombinant:

    Article Title: Unravelling the Reduction Pathway as an Alternative Metabolic Route to Hydroxycinnamate Decarboxylation in Lactobacillus plantarum
    Article Snippet: .. The supernatants of the HcrA, HcrB, and HcrAB proteins were analyzed for hydroxycinnamate reductase by adding 1.5 mM m -coumaric acid and incubating at 37°C for 16 h. For purification of the recombinant His6 -tagged proteins, the filtered supernatants were applied to a TALON Superflow resin (Clontech) equilibrated with the buffer described previously containing 0.3 M NaCl and 10 mM imidazole to improve the interaction specificity in the affinity chromatography step. .. The purity of the enzymes was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer.

    SDS Page:

    Article Title: Ethylphenol Formation by Lactobacillus plantarum: Identification of the Enzyme Involved in the Reduction of Vinylphenols
    Article Snippet: .. The filtered supernatant containing VprA protein was analyzed for reductase activity by adding 1.5 mM 4-vinylphenol and incubating at 37°C during 16 h. VprA protein was purified for affinity chromatography in a Talon Superflow resin (Clontech), and the purity of the enzyme was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in Tris-glycine buffer. .. Fractions containing the His6 -tagged proteins were pooled and analyzed for vinylphenol reductase activity.

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  • 95
    TaKaRa talon superflow metal affinity resin
    Talon Superflow Metal Affinity Resin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon superflow metal affinity resin/product/TaKaRa
    Average 95 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    talon superflow metal affinity resin - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

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