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TaKaRa takara dntp mix
Takara Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/takara dntp mix/product/TaKaRa
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
takara dntp mix - by Bioz Stars, 2020-04
92/100 stars

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Polymerase Chain Reaction:

Article Title: Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae
Article Snippet: .. PCR reactions (25 μl) contained 2–6 ng of total DNA, 2.5 μl Takara ExTaq PCR buffer 10X, 2 μl Takara dNTP mix (2.5 mM), 0.7 μl bovine serum albumin (BSA, 20 mg ml-1 ), 1 μl primers (10 μM), 0.125 μl Takara Ex Taq DNA Polymerase (5 U μl-1 ; TaKaRa, Shiga, Japan) and nuclease-free water. .. Samples were amplified in triplicate using a PCR protocol that included an initial denaturation step at 95°C (3 min), followed by 35 cycles of 95°C (30 s), 52°C (40 s) and 72°C (90 s), followed by a final extension 72°C (12 min).

Sequencing:

Article Title: Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae
Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing ... PCR reactions (25 μl) contained 2–6 ng of total DNA, 2.5 μl Takara ExTaq PCR buffer 10X, 2 μl Takara dNTP mix (2.5 mM), 0.7 μl bovine serum albumin (BSA, 20 mg ml-1 ), 1 μl primers (10 μM), 0.125 μl Takara Ex Taq DNA Polymerase (5 U μl-1 ; TaKaRa, Shiga, Japan) and nuclease-free water.

Amplification:

Article Title: Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae
Article Snippet: Paragraph title: 16S rRNA gene amplification and sequencing ... PCR reactions (25 μl) contained 2–6 ng of total DNA, 2.5 μl Takara ExTaq PCR buffer 10X, 2 μl Takara dNTP mix (2.5 mM), 0.7 μl bovine serum albumin (BSA, 20 mg ml-1 ), 1 μl primers (10 μM), 0.125 μl Takara Ex Taq DNA Polymerase (5 U μl-1 ; TaKaRa, Shiga, Japan) and nuclease-free water.

Purification:

Article Title: Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae
Article Snippet: PCR reactions (25 μl) contained 2–6 ng of total DNA, 2.5 μl Takara ExTaq PCR buffer 10X, 2 μl Takara dNTP mix (2.5 mM), 0.7 μl bovine serum albumin (BSA, 20 mg ml-1 ), 1 μl primers (10 μM), 0.125 μl Takara Ex Taq DNA Polymerase (5 U μl-1 ; TaKaRa, Shiga, Japan) and nuclease-free water. .. Triplicates were then pooled and purified using the SPRI magnetic bead, AgencourtAMPure XP PCR purification system (Beckman Coulter, Brea, CA, USA).

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  • 93
    TaKaRa telomeric repeat amplification protocol trap lig assay dntp mix
    The Pt-tripod binds the intramolecular hybrid-1 human <t>telomeric</t> G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the <t>TRAP-LIG</t> assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3
    Telomeric Repeat Amplification Protocol Trap Lig Assay Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/telomeric repeat amplification protocol trap lig assay dntp mix/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    telomeric repeat amplification protocol trap lig assay dntp mix - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    TaKaRa pcr mixture iii
    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and <t>PCR.</t> ( d ) Amplification efficiency was strongly affected by the presence of SuperScript <t>III.</t> The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.
    Pcr Mixture Iii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture iii/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr mixture iii - by Bioz Stars, 2020-04
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    99
    TaKaRa pcr mixture ii
    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified <t>cDNA</t> molecules in single cells was measured after the 2nd <t>PCR.</t> ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).
    Pcr Mixture Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture ii/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr mixture ii - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    TaKaRa deoxynucleotide triphosphates mix dntps
    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified <t>cDNA</t> molecules in single cells was measured after the 2nd <t>PCR.</t> ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).
    Deoxynucleotide Triphosphates Mix Dntps, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxynucleotide triphosphates mix dntps/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    deoxynucleotide triphosphates mix dntps - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Journal: Nature Communications

    Article Title: Solution structures of multiple G-quadruplex complexes induced by a platinum(II)-based tripod reveal dynamic binding

    doi: 10.1038/s41467-018-05810-4

    Figure Lengend Snippet: The Pt-tripod binds the intramolecular hybrid-1 human telomeric G-quadruplex and inhibits telomerase activity. a The chemical structure of the Pt-tripod with proton numbering. b The folding topology of the hybrid-1 human telomeric G-quadruplex adopted by Tel26 in K + solution 44 . Deep gray box = ( syn ) guanine, light gray box = ( anti ) guanine. c Imino proton regions of the 1D 1 H NMR titration spectra of Tel26 interacting with Pt-tripod in 100 mM K + , pH 7.0 solution, at 25 °C. Ratios of Pt-tripod/Tel26 are shown in the spectra. The imino assignments are labeled. Peaks arising from the new complexes are marked with asterisks. d CD spectra of the Tel26 titrated by the Pt-tripod in 100 mM K + solution. Ratios of Pt-tripod/Tel26 are shown in the spectra. e The Pt-tripod showed excellent telomerase inhibition property by the TRAP-LIG assay. The band labeled as ITAS is an internal control primer. The IC 50 value is determined to be 1.22 ± 0.10 μM. Error value represents the standard deviation, n = 3

    Article Snippet: Telomeric repeat amplification protocol (TRAP-LIG) assay dNTP mix, RNase inhibitor, and Taq polymerase were purchased from TaKaRa Biotechnology.

    Techniques: Activity Assay, Nuclear Magnetic Resonance, Titration, Labeling, Inhibition, Standard Deviation

    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Article Snippet: Another 15 cycles of PCR was then performed on the four fractions, each containing 1 μl of the 50 -μl purified PCR products together with 49 μl of PCR mixture III (5-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 1-μM AUP1, 1-μM AUP2 and 2.5-U TaKaRa ExTaqTM HS).

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Purification, Polymerase Chain Reaction, Produced

    Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified cDNA molecules in single cells was measured after the 2nd PCR. ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Single-cell analysis with the proposed bead-based method. ( a ) Linear relation between gene expression levels and number of cells (average number of mRNA molecules) in pools. The error bars are independent amplification replicates (single cell: mean ± SD, n = 10; 5–100 cells: mean ± SD, n = 3). ( b ) Cell-to-cell variations in gene expression among single cells. The number of amplified cDNA molecules in single cells was measured after the 2nd PCR. ( c ) Relative standard deviations of the amplification factor for each of four genes (EEF1G, B2M, TBP and SDHA) ( n = 15) and gene expression levels in single cells ( n = 30). The fluctuation of biases for every trial is smaller than the fluctuation of gene expression levels in single cells. ( d ) Relative amplification bias for four genes. Gene expressions of 4 genes in 30 unamplified bead-supported single-cell cDNA libraries and those in 10 amplified single-cell cDNA libraries were quantitatively analysed by qPCR. The amplification factors for the four genes were obtained by averaging the ratios of the cDNA copies after the amplification ( n = 10) to those before the amplification ( n = 30). The geometric means were calculated to obtain the amplification bias (amplification bias: the ratio of an amplification factor to the geometric mean).

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Single-cell Analysis, Expressing, Amplification, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Reduction of primer-dimer production by introducing VN sequence to UP2 primers. Although the production of primer-dimers by PCR is a big issue in terms of whole-cDNA amplification, it is greatly reduced by the use of UP2 primers with poly(T) plus VN sequences at 3′ termini. Electrophoregrams of PCR products obtained with two different primers (denoted as 1 and 2 in the figures) for the 2nd-strand synthesis are shown in figures ( a1–c2 ). In the figures, ‘a’, ‘b’ and ‘c’ stand for crude 1st PCR products, purified 1st PCR products and 2nd PCR products, respectively. The primers used in the cases of Figures a1, b1 and c1 were UP2 primer having poly(T) sequence at 3′ termini. The primers used in the cases of Figures a2, b2 and c2 were anchored UP2 primers having poly(T) plus VN sequences at the 3′ termini. ( d ) The number of amplified cDNA molecules (EEF1G gene) after the 1st PCR, as well as the 2nd PCR, obtained with the two kinds of primers for 2nd-strand synthesis.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Reduction of primer-dimer production by introducing VN sequence to UP2 primers. Although the production of primer-dimers by PCR is a big issue in terms of whole-cDNA amplification, it is greatly reduced by the use of UP2 primers with poly(T) plus VN sequences at 3′ termini. Electrophoregrams of PCR products obtained with two different primers (denoted as 1 and 2 in the figures) for the 2nd-strand synthesis are shown in figures ( a1–c2 ). In the figures, ‘a’, ‘b’ and ‘c’ stand for crude 1st PCR products, purified 1st PCR products and 2nd PCR products, respectively. The primers used in the cases of Figures a1, b1 and c1 were UP2 primer having poly(T) sequence at 3′ termini. The primers used in the cases of Figures a2, b2 and c2 were anchored UP2 primers having poly(T) plus VN sequences at the 3′ termini. ( d ) The number of amplified cDNA molecules (EEF1G gene) after the 1st PCR, as well as the 2nd PCR, obtained with the two kinds of primers for 2nd-strand synthesis.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification, Purification

    Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Affect of residual RT reagents on subsequent reactions. The number of cDNA molecules was evaluated by qPCR. Error bars are independent amplification replicates. ( a ) The residual RT reagents in a poly(A)-tailing reaction mixture containing bead-supported cDNA made biases large and amplification factors small (red column). The difficulty was overcome by washing beads (green column). ( b ) The addition of RT reagents to purified model cDNAs free in a poly(A)-tailing reaction mixture made biases large and amplification factors small as well. After purifying model cDNA with a purification kit, RT reagents were added to cDNA (red column) or not added (green column). ( c ) RT reagents did not affect the 2nd-strand cDNA synthesis and PCR. ( d ) Amplification efficiency was strongly affected by the presence of SuperScript III. The 11 components in an RT reagent kit were, respectively, added to 11 purified RT samples before poly(A) tailing. The 1st PCR products were evaluated by qPCR. Although the original amounts of samples were the same, the product produced by adding SuperScript III was small.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Purification, Polymerase Chain Reaction, Produced

    Change of products produced with 10 7 beads immobilizing RT primers with various densities. Starting material in all cases was 2 pg of mRNA. The error bars are independent amplification replicates. ( a ) Number of amplified cDNA molecules (EEF1G gene) in cDNA libraries after 2nd PCR. ( b ) Number of cDNA molecules (EEF1G gene) produced on beads in RT. ( c ) Electrophoregrams of crude 1st PCR products. ( d ) Electrophoregrams after purifying the crude 1st PCR products showed in the electrophoregrams of graph ‘c’.

    Journal: Nucleic Acids Research

    Article Title: Non-biased and efficient global amplification of a single-cell cDNA library

    doi: 10.1093/nar/gkt965

    Figure Lengend Snippet: Change of products produced with 10 7 beads immobilizing RT primers with various densities. Starting material in all cases was 2 pg of mRNA. The error bars are independent amplification replicates. ( a ) Number of amplified cDNA molecules (EEF1G gene) in cDNA libraries after 2nd PCR. ( b ) Number of cDNA molecules (EEF1G gene) produced on beads in RT. ( c ) Electrophoregrams of crude 1st PCR products. ( d ) Electrophoregrams after purifying the crude 1st PCR products showed in the electrophoregrams of graph ‘c’.

    Article Snippet: Global amplification of a cDNA library was then carried out after adding 19 μl of PCR mixture II (1.9-μl 10 × EX Taq Buffer, 0.25-mM dNTP, 2.2-μM UP1 and 0.95-U TaKaRa ExTaqTM HS) to the fraction.

    Techniques: Produced, Amplification, Polymerase Chain Reaction