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Trevigen tacs annexin v apoptosis detection kit
Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to <t>apoptosis</t> and cell cycle arrest. (A) Increase in apoptotic cells as determined by the <t>TACS</t> <t>annexin</t> V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.
Tacs Annexin V Apoptosis Detection Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tacs annexin v apoptosis detection kit/product/Trevigen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
tacs annexin v apoptosis detection kit - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "Regulation of the Resident Chromosomal Copy of c-myc by c-Myb Is Involved in Myeloid Leukemogenesis"

Article Title: Regulation of the Resident Chromosomal Copy of c-myc by c-Myb Is Involved in Myeloid Leukemogenesis

Journal: Molecular and Cellular Biology

doi:

Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to apoptosis and cell cycle arrest. (A) Increase in apoptotic cells as determined by the TACS annexin V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.
Figure Legend Snippet: Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to apoptosis and cell cycle arrest. (A) Increase in apoptotic cells as determined by the TACS annexin V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.

Techniques Used: Activation Assay, Apoptosis Assay, Standard Deviation, DNA Fragmentation Assay, DNA Laddering, Clone Assay, Flow Cytometry

Related Articles

Staining:

Article Title: Detection and Prevention of Ocular Phototoxicity of Ciprofloxacin and Other Fluoroquinolone Antibiotics †
Article Snippet: After UVA treatment for 10 minutes (2.5 J/cm2 ), the cells were harvested by trypsinization and collected by centrifugation at 300 g for 5 min at room temperature. .. Cells were washed with cold PBS and stained with annexin V-FITC and propidium iodide (PI) using a TACS™ Apoptosis Detection Kit (Trevigen, Gaithersburg, MD) according to the manufacturer's instructions. .. Cells positive for PI, for annexin V-FITC, or for both were quantified by flow cytometry using a Becton Dickinson FACSort (Becton Dickinson, Mountain View, CA).

Article Title: Phenyl sulfate, indoxyl sulfate and p-cresyl sulfate decrease glutathione level to render cells vulnerable to oxidative stress in renal tubular cells
Article Snippet: .. These non-adherent cells and adherent cells were combined, and stained with FITC-conjugated annexin V and propidium iodide (TACS™ Annexin V-FITC Apoptosis Detection Kit, Trevigen, Gaithersburg, MD, USA). .. The stained cells were detected using a flow cytometer (FACS Calibur™ , Becton, Dickinson and Company, Franklin Lakes, NJ, USA) and the data were analyzed using the FlowJo™ software (ver.

Article Title: Enhanced Photodynamic Efficacy towards Melanoma Cells by Encapsulation of Pc4 in Silica Nanoparticles
Article Snippet: After treatment, the cells were harvested by trypsinization and collected by centrifugation at 300 g for 5 min at room temperature. .. Cells were washed with cold phosphate buffered saline (PBS) and stained with Annexin V-FITC and propidium iodide (PI) using a TACS™ Apoptosis Detection Kit according to the manufacturer's instructions (Trevigen, Gaithersburg, MD). .. Cells positive for PI, for Annexin V-FITC, or for both were quantified by flow cytometry using a Becton Dickinson FACSort (Becton Dickinson, Mountain View, CA).

Article Title: Krüppel-like factor 9 (KLF9) prevents colorectal cancer through inhibition of interferon-related signaling
Article Snippet: HT29 cells were incubated for 24h in 10% fetal bovine serum-containing McCoy’s modified medium, followed by a 48-h treatment with growth medium alone or growth medium with added 100ng/ml ISG15 (Sino Biological, Beijing, China), 50 μM 5-fluorouracil (Teva Pharmaceuticals, Petah Tikva, Israel) or both ISG15 and 5-fluorouracil. .. Cells were harvested, rinsed in phosphate-buffered saline, suspended in the same buffer and stained using the TACS™ Annexin V-FITC Apoptosis Detection Kit (Trevigen, Gaithersburg, MD). .. Cells were subjected to flow cytometry in a BD LSRFortessa Flow Cytometer (BD Biosciences).

Binding Assay:

Article Title: Hyperactivation of PARP Triggers Nonhomologous End-Joining in Repair-Deficient Mouse Fibroblasts
Article Snippet: .. After centrifuging and washing with PBS, annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) were added in binding buffer as suggested by the manufacturer (TACS™ Annexin V Apoptosis Detection Kit, Trevigen). .. The samples were incubated at room temperature in the dark for 15 min, read on a FACS flow cytometer, and analyzed using Cell Quest software (Becton-Dickinson Immunocytometry Systems, San Jose, CA).

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    Trevigen tacs annexin v fitc apoptosis detection kit
    C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of <t>apoptosis</t> was evaluated using <t>Annexin</t> V <t>FITC</t> Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and NAC suppressed the apoptosis. N -acetylcystein (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P
    Tacs Annexin V Fitc Apoptosis Detection Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tacs annexin v fitc apoptosis detection kit/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tacs annexin v fitc apoptosis detection kit - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Trevigen tacs annexin v apoptosis detection kit
    Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to <t>apoptosis</t> and cell cycle arrest. (A) Increase in apoptotic cells as determined by the <t>TACS</t> <t>annexin</t> V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.
    Tacs Annexin V Apoptosis Detection Kit, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tacs annexin v apoptosis detection kit/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tacs annexin v apoptosis detection kit - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Trevigen tacs annexin v fitc apoptosis detection kits
    Effect of SP600125 on the induction of <t>apoptosis</t> and necrosis in MDA-MB-231 cells treated with As III combined with Tetra. After treatment with 10 μM As III +6.4 μM Tetra in the presence or absence of 10 μM of SP600125 and its negative control SP600125NC for 48 h, cells were stained with annexin <t>V-FITC</t> and PI, and analyzed by flow cytometry. (A) Representative dot plots from three independent experiments are shown. The percentages of apoptotic cells (B) and necrotic cells (C) were quantified by the same manner as described in the legend of Figures 2 and 3. *p
    Tacs Annexin V Fitc Apoptosis Detection Kits, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tacs annexin v fitc apoptosis detection kits/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tacs annexin v fitc apoptosis detection kits - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of apoptosis was evaluated using Annexin V FITC Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and NAC suppressed the apoptosis. N -acetylcystein (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P

    Journal: Frontiers in Pharmacology

    Article Title: CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure

    doi: 10.3389/fphar.2017.00207

    Figure Lengend Snippet: C-C chemokine receptor type 3 antagonists suppress the death of 661W cells induced by light exposure. (A–C) Two types of CCR3 antagonists, SB 328437 and SB 297006, were used to examine the role played by CCR3 in the death of 661W cells induced by light exposure. Both CCR3 antagonists reduced the rate of cell death. (D,E) The rate of apoptosis was evaluated using Annexin V FITC Apoptosis Detection kit with Annexin V (green), PI (red) and Hoechst 33342 (blue). The rate of annexin V-positive cells was increased in vehicle-treated group by light exposure. SB 297006 and NAC suppressed the apoptosis. N -acetylcystein (NAC) was used for a positive control. Data are expressed as mean ± SEM ( n = 6). ## indicates P

    Article Snippet: For assay of apoptosis, TACS Annexin V FITC Apoptosis Detection kit (Trevigen Inc., Gaithersburg, MD, USA) are used according to manufacturer’s protocol.

    Techniques: Positive Control

    ISG15 reduces apoptosis in HT29 cells. ( A – D ) Representative flow cytometry analysis of Annexin V-FITC staining in HT29 cells after 48-h treatment with either control medium ( A ), 50 μM 5-fluorouracil ( B ), 100ng/ml ISG15 ( C ) or ISG15 + 5-fluorouracil

    Journal: Carcinogenesis

    Article Title: Krüppel-like factor 9 (KLF9) prevents colorectal cancer through inhibition of interferon-related signaling

    doi: 10.1093/carcin/bgv104

    Figure Lengend Snippet: ISG15 reduces apoptosis in HT29 cells. ( A – D ) Representative flow cytometry analysis of Annexin V-FITC staining in HT29 cells after 48-h treatment with either control medium ( A ), 50 μM 5-fluorouracil ( B ), 100ng/ml ISG15 ( C ) or ISG15 + 5-fluorouracil

    Article Snippet: Cells were harvested, rinsed in phosphate-buffered saline, suspended in the same buffer and stained using the TACS™ Annexin V-FITC Apoptosis Detection Kit (Trevigen, Gaithersburg, MD).

    Techniques: Flow Cytometry, Cytometry, Staining

    Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to apoptosis and cell cycle arrest. (A) Increase in apoptotic cells as determined by the TACS annexin V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.

    Journal: Molecular and Cellular Biology

    Article Title: Regulation of the Resident Chromosomal Copy of c-myc by c-Myb Is Involved in Myeloid Leukemogenesis

    doi:

    Figure Lengend Snippet: Activation of MybEnER in clonal populations of M1 and RI-4-11 cells leads to apoptosis and cell cycle arrest. (A) Increase in apoptotic cells as determined by the TACS annexin V apoptosis assay. The y axis depicts the fold increase in the amount of apoptotic cells, and the x axis depicts the hours after stimulation with 4-OHT. Data shown are the mean of three independent results with a standard deviation smaller than 15%. (B) A DNA fragmentation assay used to detect late-stage programmed cell death. DNA laddering is prominent in MybEnER clones following activation of the fusion protein. (C) Flow microfluorometric analysis of the cell cycle distribution in RI-4-11/MybEnER or RI-4-11/EnER cells stimulated with 4-OHT for 24 h. Cell cycle distribution was examined using a Becton Dickinson FACScan and analyzed with the ModFitLT V2.0 program. The proportions of cells in G 0 -G 1 , G 2 -M, and S are shown in boxes in each graph as percentages of the total population.

    Article Snippet: Early-stage apoptosis was detected using the TACS annexin V apoptosis detection kit (Trevigen, Inc.) according to the manufacturer's instructions.

    Techniques: Activation Assay, Apoptosis Assay, Standard Deviation, DNA Fragmentation Assay, DNA Laddering, Clone Assay, Flow Cytometry

    Effect of SP600125 on the induction of apoptosis and necrosis in MDA-MB-231 cells treated with As III combined with Tetra. After treatment with 10 μM As III +6.4 μM Tetra in the presence or absence of 10 μM of SP600125 and its negative control SP600125NC for 48 h, cells were stained with annexin V-FITC and PI, and analyzed by flow cytometry. (A) Representative dot plots from three independent experiments are shown. The percentages of apoptotic cells (B) and necrotic cells (C) were quantified by the same manner as described in the legend of Figures 2 and 3. *p

    Journal: Frontiers in Pharmacology

    Article Title: JNK and Autophagy Independently Contributed to Cytotoxicity of Arsenite combined With Tetrandrine via Modulating Cell Cycle Progression in Human Breast Cancer Cells

    doi: 10.3389/fphar.2020.01087

    Figure Lengend Snippet: Effect of SP600125 on the induction of apoptosis and necrosis in MDA-MB-231 cells treated with As III combined with Tetra. After treatment with 10 μM As III +6.4 μM Tetra in the presence or absence of 10 μM of SP600125 and its negative control SP600125NC for 48 h, cells were stained with annexin V-FITC and PI, and analyzed by flow cytometry. (A) Representative dot plots from three independent experiments are shown. The percentages of apoptotic cells (B) and necrotic cells (C) were quantified by the same manner as described in the legend of Figures 2 and 3. *p

    Article Snippet: Annexin V/PI Analysis The TACS™ Annexin V-FITC apoptosis detection Kits (Trevigen, MD, USA) was used for the detection of apoptotic and necrotic cells according to the method described previously ( ; ).

    Techniques: Multiple Displacement Amplification, Negative Control, Staining, Flow Cytometry

    Effects of autophagy inhibitors on the induction of apoptosis and necrosis in MDA-MB-231 cells treated with the combination of As III and Tetra. After treatment with 10 μM As III +6.4 μM Tetra in the presence or absence of 3-MA (0.25, 1.0 mM) or wortmannin (0.25, 1.0 μM) for 48 h, cells were stained with annexin V-FITC and PI, and analyzed by flow cytometry. The percentages of apoptotic cells (A) and necrotic cells (B) were quantified by the same manner as described in the legend of Figures 2 and 3 . *p

    Journal: Frontiers in Pharmacology

    Article Title: JNK and Autophagy Independently Contributed to Cytotoxicity of Arsenite combined With Tetrandrine via Modulating Cell Cycle Progression in Human Breast Cancer Cells

    doi: 10.3389/fphar.2020.01087

    Figure Lengend Snippet: Effects of autophagy inhibitors on the induction of apoptosis and necrosis in MDA-MB-231 cells treated with the combination of As III and Tetra. After treatment with 10 μM As III +6.4 μM Tetra in the presence or absence of 3-MA (0.25, 1.0 mM) or wortmannin (0.25, 1.0 μM) for 48 h, cells were stained with annexin V-FITC and PI, and analyzed by flow cytometry. The percentages of apoptotic cells (A) and necrotic cells (B) were quantified by the same manner as described in the legend of Figures 2 and 3 . *p

    Article Snippet: Annexin V/PI Analysis The TACS™ Annexin V-FITC apoptosis detection Kits (Trevigen, MD, USA) was used for the detection of apoptotic and necrotic cells according to the method described previously ( ; ).

    Techniques: Multiple Displacement Amplification, Staining, Flow Cytometry