shuffle t7 competent e coli  (New England Biolabs)


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    New England Biolabs shuffle t7 competent e coli
    Shuffle T7 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
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    shuffle t7 competent e coli - by Bioz Stars, 2023-03
    96/100 stars

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    shuffle t7 e coli  (New England Biolabs)


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    New England Biolabs shuffle t7 e coli
    Shuffle T7 E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 e coli/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    e coli shuffle t7 express  (New England Biolabs)


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    New England Biolabs e coli shuffle t7 express
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli shuffle t7 express/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli shuffle t7 express - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes"

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    Journal: bioRxiv

    doi: 10.1101/2023.02.24.529993

    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Figure Legend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Techniques Used: Sequencing


    Figure Legend Snippet:

    Techniques Used:

    Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    e coli shuffle t7  (New England Biolabs)


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    New England Biolabs e coli shuffle t7
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli shuffle t7/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli shuffle t7 - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes"

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    Journal: bioRxiv

    doi: 10.1101/2023.02.24.529993

    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Figure Legend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Techniques Used: Sequencing


    Figure Legend Snippet:

    Techniques Used:

    Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    shuffle t7 express  (New England Biolabs)


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    New England Biolabs shuffle t7 express
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Shuffle T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 express/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    shuffle t7 express - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes"

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    Journal: bioRxiv

    doi: 10.1101/2023.02.24.529993

    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Figure Legend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Techniques Used: Sequencing

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.
    Figure Legend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Techniques Used: Plasmid Preparation, Imaging

    e coli shuffle t7 express lysy  (New England Biolabs)


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    New England Biolabs e coli shuffle t7 express lysy
    E Coli Shuffle T7 Express Lysy, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86/100 stars

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    e coli shuffle t7 cells  (New England Biolabs)


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    New England Biolabs e coli shuffle t7 cells
    E Coli Shuffle T7 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    shuffle t7 competent e coli cells  (New England Biolabs)


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    New England Biolabs shuffle t7 competent e coli cells
    Shuffle T7 Competent E Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 competent e coli cells/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
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    shuffle t7 express e coli strain  (New England Biolabs)


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    New England Biolabs shuffle t7 express e coli strain
    Shuffle T7 Express E Coli Strain, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 express e coli strain/product/New England Biolabs
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    t7 shuffle  (New England Biolabs)


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    New England Biolabs t7 shuffle
    T7 Shuffle, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 shuffle/product/New England Biolabs
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    shuffle t7 competent e coli  (New England Biolabs)


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    New England Biolabs shuffle t7 competent e coli
    Shuffle T7 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 competent e coli/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
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    shuffle t7 competent e coli - by Bioz Stars, 2023-03
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    • shuffle t7 competent e coli  (New England Biolabs)
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    New England Biolabs shuffle t7 competent e coli
    Shuffle T7 Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 competent e coli/product/New England Biolabs
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    shuffle t7 e coli  (New England Biolabs)
    86
    New England Biolabs shuffle t7 e coli
    Shuffle T7 E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shuffle t7 e coli/product/New England Biolabs
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    e coli shuffle t7 express  (New England Biolabs)
    86
    New England Biolabs e coli shuffle t7 express
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli shuffle t7 express/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli shuffle t7 express - by Bioz Stars, 2023-03
    86/100 stars
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    e coli shuffle t7  (New England Biolabs)
    86
    New England Biolabs e coli shuffle t7
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli shuffle t7/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli shuffle t7 - by Bioz Stars, 2023-03
    86/100 stars
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    shuffle t7 express  (New England Biolabs)
    86
    New England Biolabs shuffle t7 express
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Shuffle T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli shuffle t7 express lysy  (New England Biolabs)
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    New England Biolabs e coli shuffle t7 express lysy
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7 Express Lysy, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli shuffle t7 cells  (New England Biolabs)
    86
    New England Biolabs e coli shuffle t7 cells
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    E Coli Shuffle T7 Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    shuffle t7 competent e coli cells  (New England Biolabs)
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    New England Biolabs shuffle t7 competent e coli cells
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Shuffle T7 Competent E Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    shuffle t7 express e coli strain  (New England Biolabs)
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    New England Biolabs shuffle t7 express e coli strain
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
    Shuffle T7 Express E Coli Strain, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t7 shuffle  (New England Biolabs)
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    New England Biolabs t7 shuffle
    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. <t>[T7</t> = T7 express lysY/l q , SHuffle = SHuffle T7 express]
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    Image Search Results


    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Article Snippet: Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. #C3029), T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3) (NEB, cat. #C2527), BL21 (NEB, cat. #C2530), and NEB5α (NEB, cat. #C2987) via heat shock as described above.

    Techniques: Sequencing

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet:

    Article Snippet: Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. #C3029), T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3) (NEB, cat. #C2527), BL21 (NEB, cat. #C2530), and NEB5α (NEB, cat. #C2987) via heat shock as described above.

    Techniques:

    Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. #C3029), T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3) (NEB, cat. #C2527), BL21 (NEB, cat. #C2530), and NEB5α (NEB, cat. #C2987) via heat shock as described above.

    Techniques: Plasmid Preparation, Imaging

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. #C3029), T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3) (NEB, cat. #C2527), BL21 (NEB, cat. #C2530), and NEB5α (NEB, cat. #C2987) via heat shock as described above.

    Techniques: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: Plasmid DNA from confirmed clones was purified using the GeneJet Plasmid Miniprep Kit (ThermoFisher Scientific; Waltham, MA; cat. #K0503) and transferred into E. coli SHuffle T7 express (NEB, cat. #C3029), T7 express lysY/lq (NEB, cat. #C3013), BL21(DE3) (NEB, cat. #C2527), BL21 (NEB, cat. #C2530), and NEB5α (NEB, cat. #C2987) via heat shock as described above.

    Techniques: Plasmid Preparation, Imaging

    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Article Snippet: E. coli BL21(DE3) and T7 express lysY/l q were induced at 37°C and 200 rpm shaking, while E. coli SHuffle T7 express was induced at 30°C, 200 rpm, based on the manufacturer’s recommendations (NEB).

    Techniques: Sequencing

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet:

    Article Snippet: E. coli BL21(DE3) and T7 express lysY/l q were induced at 37°C and 200 rpm shaking, while E. coli SHuffle T7 express was induced at 30°C, 200 rpm, based on the manufacturer’s recommendations (NEB).

    Techniques:

    Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of E. coli B-strains without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr3 (“+ pETI7b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: E. coli BL21(DE3) and T7 express lysY/l q were induced at 37°C and 200 rpm shaking, while E. coli SHuffle T7 express was induced at 30°C, 200 rpm, based on the manufacturer’s recommendations (NEB).

    Techniques: Plasmid Preparation, Imaging

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: E. coli BL21(DE3) and T7 express lysY/l q were induced at 37°C and 200 rpm shaking, while E. coli SHuffle T7 express was induced at 30°C, 200 rpm, based on the manufacturer’s recommendations (NEB).

    Techniques: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: E. coli BL21(DE3) and T7 express lysY/l q were induced at 37°C and 200 rpm shaking, while E. coli SHuffle T7 express was induced at 30°C, 200 rpm, based on the manufacturer’s recommendations (NEB).

    Techniques: Plasmid Preparation, Imaging

    Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Alignment of genes and matching amino acid sequences was performed by Clustal Omega . Sequences from nucleotides 22 – 357, 386 – 960, and 1014 – 1080 were omitted because the strains shared 100% sequence identity for each of these regions. Base changes are highlighted; coloring indicates which base change correlates with which amino acid. Refer to materials & methods section for details on how the sequences were acquired. [T7 = T7 express lysY/l q , SHuffle = SHuffle T7 express]

    Article Snippet: The pmrA and pmrB genes encoded by strains used in this paper were identified using BLAST , by querying the sequences against the genomes of NEB strains E. coli BL21 (Accession No. CP053601 ), BL21(DE3) (Accession No. CP053602 ), T7 express lysY/l q (Accession No. CP053595 ), SHuffle T7 Express (Accession No. CP014269 ), and NEB5α (Accession No. CP017100.1 ).

    Techniques: Sequencing

    Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of E. coli strains without T7 RNA polymerase without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b- mcr-3 (“+ pET17b-mcr-3”) on 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: The pmrA and pmrB genes encoded by strains used in this paper were identified using BLAST , by querying the sequences against the genomes of NEB strains E. coli BL21 (Accession No. CP053601 ), BL21(DE3) (Accession No. CP053602 ), T7 express lysY/l q (Accession No. CP053595 ), SHuffle T7 Express (Accession No. CP014269 ), and NEB5α (Accession No. CP017100.1 ).

    Techniques: Plasmid Preparation, Imaging

    Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Journal: bioRxiv

    Article Title: Escherichia coli B-strains are intrinsically resistant to colistin and not suitable for characterization and identification of mcr genes

    doi: 10.1101/2023.02.24.529993

    Figure Lengend Snippet: Plating assay of exponentially growing E. coli B strains BL21D and T7 without plasmid (“no plasmid”), with empty pET17b (“+ pET17b”), and with pET17b - mcr-3 (“+ pET17b-mc-r3”) on LB containing 1 mM MgCl 2 and 0, 0.4, or 1 mM of IPTG (0mM IPTG plates were prepared by adding distilled water instead of IPTG). Exponentially grown strains were 10-fold serial diluted in PBS. Undiluted to 10 -7 dilutions were plated in 10μL volumes. Results were consistent across three replicates and one replicate is shown here; images were acquired with the Bio-Rad ChemiDoc MP Imaging system.

    Article Snippet: The pmrA and pmrB genes encoded by strains used in this paper were identified using BLAST , by querying the sequences against the genomes of NEB strains E. coli BL21 (Accession No. CP053601 ), BL21(DE3) (Accession No. CP053602 ), T7 express lysY/l q (Accession No. CP053595 ), SHuffle T7 Express (Accession No. CP014269 ), and NEB5α (Accession No. CP017100.1 ).

    Techniques: Plasmid Preparation, Imaging