Journal: Analytical Biochemistry

Article Title: Detection of concentration-dependent conformational changes in SARS-CoV-2 nucleoprotein by agarose native gel electrophoresis

doi: 10.1016/j.ab.2022.114995

Figure Lengend Snippet: Expression of the purification of SARS-CoV-2 NP. A. Flowchart of the expression and purification The conformation predicted for AlphaFold2 is shown, where eXact tag is shown by orange and NP by blue. B. Soluble expression of codon optimized eXact-NP fusion protein in E. coli. Lane 1, molecular weight marker; Lane 2, total cell protein from E. coli T7Express pLysY/pET3d-eXact-NP before IPTG induction (0 h); Lane 3, total cell protein 3 h after induction; Lane 4, soluble fraction after induction; Lane 5, insoluble fraction after induction. The single asterisk indicates the NP fusion protein. C. Purificaion of NP by Profinity eXact affinity chromatography. Lane 1, molecular weight marker; Lane 2, soluble fraction from supernatant of lysed and centrifuged cells; Lane 3, flow-through fraction of Profinity eXact chromatography; Lane 4, wash fraction; Lane 5, products after proteolytic cleavage: the double asterisk indicates tag free NP.

Article Snippet: The construct of pET-3d-eXact-NP was transformed into E. coli T7 Express lysY/Iq (NEB).

Techniques: Expressing, Purification, Molecular Weight, Marker, Affinity Chromatography, Chromatography

Journal: Analytical Biochemistry

Article Title: Detection of concentration-dependent conformational changes in SARS-CoV-2 nucleoprotein by agarose native gel electrophoresis

doi: 10.1016/j.ab.2022.114995

Figure Lengend Snippet: Purification of SARS-CoV-2 NP fusion proteins overexpressed in E. coli T7 Express lysY/Iq.

Article Snippet: The construct of pET-3d-eXact-NP was transformed into E. coli T7 Express lysY/Iq (NEB).

Techniques: Purification, Chromatography, Size-exclusion Chromatography

Journal: Cell Reports

Article Title: Structural basis of activation and antagonism of receptor signaling mediated by interleukin-27

doi: 10.1016/j.celrep.2022.111490

Figure Lengend Snippet:

Article Snippet: E. coli BL21 T7 Express lysY/Iq , New England Biolabs , Cat #C3013I.

Techniques: Recombinant, Plasmid Preparation, Inhibition, Software

Journal: Cell Reports

Article Title: Structural basis of activation and antagonism of receptor signaling mediated by interleukin-27

doi: 10.1016/j.celrep.2022.111490

Figure Lengend Snippet:

Article Snippet: The Nb5 plasmid was transformed into T7 Express lysY/Iq cells (NEB) and selected on carbenicillin.

Techniques: Recombinant, Plasmid Preparation, Inhibition, Software