Journal: PLoS ONE

Article Title: Diversity of Plant Methionine Sulfoxide Reductases B and Evolution of a Form Specific for Free Methionine Sulfoxide

doi: 10.1371/journal.pone.0065637

Figure Lengend Snippet: Kinetic properties of soybean methionine sulfoxide reductases B.

Article Snippet: pET21b, pDEST17 and pET15b carrying appropriate coding sequences were transformed into E. coli BL21 (DE3) T7 Express® (New England Biolabs) and cultured in media containing recommended concentrations of antibiotics.

Techniques: Activity Assay

Journal: Nature Communications

Article Title: Unraveling function and diversity of bacterial lectins in the human microbiome

doi: 10.1038/s41467-022-29949-3

Figure Lengend Snippet: Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).

Article Snippet: Plasmids were transformed into E. coli BL21(DE3) T7 Express cells (New England Biolabs), and glycerol freezer stocks were prepared from single colonies.

Techniques: Plasmid Preparation, Flow Cytometry, MANN-WHITNEY

Journal: iScience

Article Title: Closantel is an allosteric inhibitor of human Taspase1

doi: 10.1016/j.isci.2021.103524

Figure Lengend Snippet: Key resources table

Article Snippet: T7 express competent E. coli , BL21 DE3 derivative , NEB , NEB Cat# C2566.

Techniques: Recombinant, Labeling, Protease Inhibitor, Western Blot, Clone Assay, Expressing, HTRF Assay, Derivative Assay, Plasmid Preparation, Software, Inhibition, Fluorescence, Marker, Blocking Assay