bl21 de3 t7 express competent e coli  (New England Biolabs)


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    New England Biolabs bl21 de3 t7 express competent e coli
    Bl21 De3 T7 Express Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bl21 de3 t7 express competent e coli  (New England Biolabs)


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    New England Biolabs bl21 de3 t7 express competent e coli
    Bl21 De3 T7 Express Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bl21 de3 t7 express competent e coli  (New England Biolabs)


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    New England Biolabs bl21 de3 t7 express competent e coli
    Bl21 De3 T7 Express Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli bl21 de3 t7 express  (New England Biolabs)


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    New England Biolabs e coli bl21 de3 t7 express
    Kinetic properties of soybean methionine sulfoxide reductases B.
    E Coli Bl21 De3 T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Diversity of Plant Methionine Sulfoxide Reductases B and Evolution of a Form Specific for Free Methionine Sulfoxide"

    Article Title: Diversity of Plant Methionine Sulfoxide Reductases B and Evolution of a Form Specific for Free Methionine Sulfoxide

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065637

    Kinetic properties of soybean methionine sulfoxide reductases B.
    Figure Legend Snippet: Kinetic properties of soybean methionine sulfoxide reductases B.

    Techniques Used: Activity Assay

    e coli bl21 de3 plyse s  (New England Biolabs)


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    New England Biolabs e coli bl21 de3 plyse s
    E Coli Bl21 De3 Plyse S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bl21 de3 plyss  (New England Biolabs)


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    New England Biolabs bl21 de3 plyss
    Plasmids and strain used in this study
    Bl21 De3 Plyss, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion"

    Article Title: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

    Journal: Biotechnology and bioengineering

    doi: 10.1002/bit.27440

    Plasmids and strain used in this study
    Figure Legend Snippet: Plasmids and strain used in this study

    Techniques Used:

    Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density
    Figure Legend Snippet: Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density

    Techniques Used: Concentration Assay

    Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density
    Figure Legend Snippet: Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density

    Techniques Used: Expressing, Cytometry, Plasmid Preparation, Fluorescence

    bl21 de3 plyss  (New England Biolabs)


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    New England Biolabs bl21 de3 plyss
    Plasmids and strain used in this study
    Bl21 De3 Plyss, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion"

    Article Title: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

    Journal: Biotechnology and bioengineering

    doi: 10.1002/bit.27440

    Plasmids and strain used in this study
    Figure Legend Snippet: Plasmids and strain used in this study

    Techniques Used:

    Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density
    Figure Legend Snippet: Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density

    Techniques Used: Concentration Assay

    Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density
    Figure Legend Snippet: Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density

    Techniques Used: Expressing, Cytometry, Plasmid Preparation, Fluorescence

    e coli bl21 de3 t7 express cells  (New England Biolabs)


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    New England Biolabs e coli bl21 de3 t7 express cells
    Wild-type 8-week C57BL/6 mice were colonized with <t>E.</t> <t>coli</t> engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).
    E Coli Bl21 De3 T7 Express Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Unraveling function and diversity of bacterial lectins in the human microbiome"

    Article Title: Unraveling function and diversity of bacterial lectins in the human microbiome

    Journal: Nature Communications

    doi: 10.1038/s41467-022-29949-3

    Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).
    Figure Legend Snippet: Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).

    Techniques Used: Plasmid Preparation, Flow Cytometry, MANN-WHITNEY

    t7 express lysy iq bl21 de3 e coli  (New England Biolabs)


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    New England Biolabs t7 express lysy iq bl21 de3 e coli
    T7 Express Lysy Iq Bl21 De3 E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t7 express escherichia coli bl21 de3 bacteria  (New England Biolabs)


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    New England Biolabs t7 express escherichia coli bl21 de3 bacteria
    T7 Express Escherichia Coli Bl21 De3 Bacteria, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli bl21 de3 plyse s  (New England Biolabs)


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    New England Biolabs e coli bl21 de3 plyse s
    E Coli Bl21 De3 Plyse S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bl21 de3 t7 express competent e coli
    Bl21 De3 T7 Express Competent E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs e coli bl21 de3 t7 express
    Kinetic properties of soybean methionine sulfoxide reductases B.
    E Coli Bl21 De3 T7 Express, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs e coli bl21 de3 plyse s
    Kinetic properties of soybean methionine sulfoxide reductases B.
    E Coli Bl21 De3 Plyse S, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bl21 de3 plyss
    Plasmids and strain used in this study
    Bl21 De3 Plyss, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs e coli bl21 de3 t7 express cells
    Wild-type 8-week C57BL/6 mice were colonized with <t>E.</t> <t>coli</t> engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).
    E Coli Bl21 De3 T7 Express Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t7 express lysy iq bl21 de3 e coli
    Wild-type 8-week C57BL/6 mice were colonized with <t>E.</t> <t>coli</t> engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).
    T7 Express Lysy Iq Bl21 De3 E Coli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t7 express escherichia coli bl21 de3 bacteria
    Wild-type 8-week C57BL/6 mice were colonized with <t>E.</t> <t>coli</t> engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).
    T7 Express Escherichia Coli Bl21 De3 Bacteria, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Kinetic properties of soybean methionine sulfoxide reductases B.

    Journal: PLoS ONE

    Article Title: Diversity of Plant Methionine Sulfoxide Reductases B and Evolution of a Form Specific for Free Methionine Sulfoxide

    doi: 10.1371/journal.pone.0065637

    Figure Lengend Snippet: Kinetic properties of soybean methionine sulfoxide reductases B.

    Article Snippet: pET21b, pDEST17 and pET15b carrying appropriate coding sequences were transformed into E. coli BL21 (DE3) T7 Express® (New England Biolabs) and cultured in media containing recommended concentrations of antibiotics.

    Techniques: Activity Assay

    Plasmids and strain used in this study

    Journal: Biotechnology and bioengineering

    Article Title: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

    doi: 10.1002/bit.27440

    Figure Lengend Snippet: Plasmids and strain used in this study

    Article Snippet: E. coli strains BL21(DE3) (Cat #C2527) and BL21(DE3) pLysS (Cat #C3010) were obtained from New England Biolabs (NEB), Ipswich, MA.

    Techniques:

    Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density

    Journal: Biotechnology and bioengineering

    Article Title: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

    doi: 10.1002/bit.27440

    Figure Lengend Snippet: Growth and byproduct formation of Escherichia coli strains in minimal media fermentations. Biomass levels and residual glucose concentration (blue) as a function of time for (a) BL21(DE3)pLys, (b) BW25113, (c) BWapldf, and (d) DLF_R002, respectively. (e) Distribution of glucose utilized during growth in minimal medium fermentations for BW25113 (white), BL21(DE3) pLys (light gray), BWapldf (dark gray), and DLF_R002 (black). Results are averages of duplicate fermentations. CO2 was explicitly measured via off-gas analysis for strain BW25113, BWapldf, and DLF_R002. In the case of BL21(DE3) pLys, CO2 is included in unknown products required to account for glucose consumption. OD, optical density

    Article Snippet: E. coli strains BL21(DE3) (Cat #C2527) and BL21(DE3) pLysS (Cat #C3010) were obtained from New England Biolabs (NEB), Ipswich, MA.

    Techniques: Concentration Assay

    Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density

    Journal: Biotechnology and bioengineering

    Article Title: Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion

    doi: 10.1002/bit.27440

    Figure Lengend Snippet: Head to head comparison of autoinduction via phosphate depletion with pET-based expression in BL21(DE3). (a) pET-based mCherry expression in BL21(DE3) with pLysS. mCherry (red lines) and biomass levels (OD600 nm, black lines) over time. Solid lines: lactose-based autoinduction. Dashed lines: IPTG induction in LB media. (b) yibDp-based mCherry expression in DLF_R002 in AB media mCherry (red lines) and biomass levels (OD600 nm, black lines). (c) Cytometry of induced populations (gray: empty vector control, red: pET-mCherry in BL21(DE3) + pLysS, green: yibDp-mCherry in DLF_R002). (d) Expression of the Lon substrate (GFP-β20-cp6) in BL21(DE3) and DLF_R002. Normalized fluorescence is relative fluorescence normalized to OD. Black line: GFP control (non-Lon substrate) in DLF_R002. Red line: BL21(DE3) expressing GFP-β20-cp6. Green line: DLF_R002 expressing GFP-β20-cp6. Shaded areas are standard deviations of at least three replicates. AB, autoinduction broth; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria broth; OD, optical density

    Article Snippet: E. coli strains BL21(DE3) (Cat #C2527) and BL21(DE3) pLysS (Cat #C3010) were obtained from New England Biolabs (NEB), Ipswich, MA.

    Techniques: Expressing, Cytometry, Plasmid Preparation, Fluorescence

    Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).

    Journal: Nature Communications

    Article Title: Unraveling function and diversity of bacterial lectins in the human microbiome

    doi: 10.1038/s41467-022-29949-3

    Figure Lengend Snippet: Wild-type 8-week C57BL/6 mice were colonized with E. coli engineered to express Cbeg5 (Treatment, EC:Cbeg5) or E. coli with an empty vector (Control, EC:Con). After 1 week of colonization the colon was analyzed for inflammatory changes by histology ( a ) and for change in immune cell populations by flow cytometry ( b , c ). a No difference was observed in colon histologic inflammatory indices (one representative image, Supplementary Fig. ). b After gating for singlets and live cells (Aqua LIVE/DEAD) the gating schema is shown for identification of macrophages MHCII + CD11c + CD64 + CD11b + and monocytes MHCII - Lyc + CD11b + . c There was a significant increase in lamina propria MHCII + CD11c + CD64 + CD11b + macrophages and a decrease in MHCII − Lyc + CD11b + monocytes in the treatment group ( N = 16 (8 M/8 F) Treatment, N = 18 (9 M/9 F) Control. Data represents 4 independent experiments. Error bars are mean ± s.e.m. Control and treatment populations compared using an unpaired, two-sided Mann–Whitney test).

    Article Snippet: Plasmids were transformed into E. coli BL21(DE3) T7 Express cells (New England Biolabs), and glycerol freezer stocks were prepared from single colonies.

    Techniques: Plasmid Preparation, Flow Cytometry, MANN-WHITNEY