t7 exonuclease digestion  (New England Biolabs)


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    Name:
    T7 Exonuclease
    Description:
    T7 Exonuclease 5 000 units
    Catalog Number:
    m0263l
    Price:
    261
    Size:
    5 000 units
    Category:
    Exonucleases
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    Structured Review

    New England Biolabs t7 exonuclease digestion
    T7 Exonuclease
    T7 Exonuclease 5 000 units
    https://www.bioz.com/result/t7 exonuclease digestion/product/New England Biolabs
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t7 exonuclease digestion - by Bioz Stars, 2020-07
    97/100 stars

    Related Products / Commonly Used Together

    t7 exonuclease

    Images

    1) Product Images from "Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *"

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.140350

    T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.
    Figure Legend Snippet: T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.

    Techniques Used: Incubation, Polyacrylamide Gel Electrophoresis

    2) Product Images from "Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *"

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.140350

    T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.
    Figure Legend Snippet: T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.

    Techniques Used: Incubation, Polyacrylamide Gel Electrophoresis

    Related Articles

    other:

    Article Title: Impact of DNA ligase IV on the fidelity of end joining in human cells
    Article Snippet: Together, these results support the notion that the presence of DNA ligase IV–XRCC4 protects DNA ends from end degradation by T7 exonuclease.

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *
    Article Snippet: T7 exonuclease and XhoI digestion suggested that the band that was unaffected following the addition of BCL2 with compatible ends was indeed circular ( C ).).

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *
    Article Snippet: Results showed that T7 exonuclease was able to digest the bands corresponding to the substrate and linear dimer DNA but not the one in between ( B , lanes 2 and 3 ) (data not shown), suggesting the circular nature of the products.

    Article Title: Impact of DNA ligase IV on the fidelity of end joining in human cells
    Article Snippet: We also show that DNA ligase IV–XRCC4 can contribute to the protection of ends from degradation by T7 exonuclease.

    Purification:

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *
    Article Snippet: .. T7 exonuclease digestion was performed by incubating purified EJ products with either increasing concentrations or 5 units of T7 exonuclease (New England Biolabs) at 25 °C for 2 h. In some cases, a fraction of EJ products was digested with XhoI (4 units) (37 °C for 4 h) prior to T7 exonuclease digestion. .. The EJ products of interest were cut out from PAGE, and DNA was eluted in a solution containing Tris (10 m m ), EDTA (1 m m ) and NaCl (0.5 m ).

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  • 97
    New England Biolabs t7 exonuclease digestion
    <t>T7</t> exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.
    T7 Exonuclease Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 exonuclease digestion/product/New England Biolabs
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t7 exonuclease digestion - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

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    T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.

    Journal: The Journal of Biological Chemistry

    Article Title: Anti-apoptotic Protein BCL2 Down-regulates DNA End Joining in Cancer Cells *

    doi: 10.1074/jbc.M110.140350

    Figure Lengend Snippet: T7 exonuclease digestion to determine the topology of end-joined products. A , diagrammatic representation of plausible end joined products by cell-free extracts. B , T7 exonuclease and XhoI digestion followed by T7 exonuclease digestion pattern of end-joined products of K562 cell-free extracts. Multiple end-joining reactions were carried out using 5′ compatible substrates, and products were incubated with either T7 exonuclease (5 units/10-μl reaction for 2 h), XhoI, or both. The products were resolved on an 8% denaturing PAGE. M , 50-nt ladder.

    Article Snippet: T7 exonuclease digestion was performed by incubating purified EJ products with either increasing concentrations or 5 units of T7 exonuclease (New England Biolabs) at 25 °C for 2 h. In some cases, a fraction of EJ products was digested with XhoI (4 units) (37 °C for 4 h) prior to T7 exonuclease digestion.

    Techniques: Incubation, Polyacrylamide Gel Electrophoresis