t4 rna ligase 2 truncated  (Thermo Fisher)


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    Name:
    SuperScript III Reverse Transcriptase
    Description:
    Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT that was created by introduction of several mutations for reduced RNase H activity increased half life and improved thermal stability SuperScript III RT offers higher cDNA yields improved cDNA lengths improved efficiency on GC rich target RNAs and overall better performance than wild type MMLV and MMLV RNase H minus enzymes SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    Catalog Number:
    18080044
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher t4 rna ligase 2 truncated
    Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT that was created by introduction of several mutations for reduced RNase H activity increased half life and improved thermal stability SuperScript III RT offers higher cDNA yields improved cDNA lengths improved efficiency on GC rich target RNAs and overall better performance than wild type MMLV and MMLV RNase H minus enzymes SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    https://www.bioz.com/result/t4 rna ligase 2 truncated/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 2 truncated - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Amplification:

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Article Title: Reprogramming the Dynamin 2 mRNA by Spliceosome-mediated RNA Trans-splicing
    Article Snippet: .. Total RNA (1 µg) was submitted to reverse transcription using the Superscript III reverse transcriptase kit (Life Technologies) using random primers. cDNA were amplified by PCR under the following conditions: 96°C for 5 minutes, cycles of 30 seconds at 96°C, 30 seconds at the appropriate temperature (58 to 61°C), 30 seconds to 1 minute at 72°C, and a final step of 7 minutes at 72°C. ..

    Quantitation Assay:

    Article Title: Identification of Cis-Acting Elements on Positive-Strand Subgenomic mRNA Required for the Synthesis of Negative-Strand Counterpart in Bovine Coronavirus
    Article Snippet: .. Quantitation of (−)-Strand sgmRNA Synthesis by RT-qPCR To assess the efficiency of (−)-strand sgmRNA synthesis from wt sBM25A and the mutants except sNL, sΔSL1 and sΔSL2, 1 µg of decapped and ligated RNA collected from BCoV-infected sgmRNA-transfected HRT-18 cells at 8 hpt was used in an RT reaction with oligonucleotide MHV3'UTR3(−) and SuperScript III reverse transcriptase (Invitrogen). .. TaqMan probe-5 ( ) used for RT-qPCR were designed by the Primer Express computer program (Applied Biosystems, Foster City, CA, USA).

    Quantitative RT-PCR:

    Article Title: Identification of Cis-Acting Elements on Positive-Strand Subgenomic mRNA Required for the Synthesis of Negative-Strand Counterpart in Bovine Coronavirus
    Article Snippet: .. Quantitation of (−)-Strand sgmRNA Synthesis by RT-qPCR To assess the efficiency of (−)-strand sgmRNA synthesis from wt sBM25A and the mutants except sNL, sΔSL1 and sΔSL2, 1 µg of decapped and ligated RNA collected from BCoV-infected sgmRNA-transfected HRT-18 cells at 8 hpt was used in an RT reaction with oligonucleotide MHV3'UTR3(−) and SuperScript III reverse transcriptase (Invitrogen). .. TaqMan probe-5 ( ) used for RT-qPCR were designed by the Primer Express computer program (Applied Biosystems, Foster City, CA, USA).

    Article Title: A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo
    Article Snippet: .. qRT-PCR Total RNA was extracted from the caudal region (below forelimb) of E9.5 Foxa2mcm ; Rosa26LSL-NICD using a Qiagen Micro Plus kit. cDNA was synthesised using SuperScript III reverse transcriptase (Life Technologies). qRT-PC was performed with Power SYBR Green Master Mix (Life Technologies) and reactions measured in a C1000 Thermal Cycler (Bio-Rad) under the following conditions: 95°C for 5 min, 40 cycles 95°C for 15 s and 60°C for 1 min. Ptch1 , Gli1 , Hes1 ( ) and cHairy2 primers (F: 5′-CCGTACCCTGCAAGCCAGGTG-3′, R: 5′-GCCCATCA-GAGGCAAGCAGCA-3′) were described previously and normalised against β-actin ( ; ) using the Pfaffl equation ( ). .. Image acquisition/analysis Fluorescent signal was acquired using a compound microscope (Leica DM5000 B), an Olympus IX70 deconvolution microscope or the Zeiss LSM-710 confocal microscope.

    Purification:

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

    SYBR Green Assay:

    Article Title: A conserved role for Notch signaling in priming the cellular response to Shh through ciliary localisation of the key Shh transducer Smo
    Article Snippet: .. qRT-PCR Total RNA was extracted from the caudal region (below forelimb) of E9.5 Foxa2mcm ; Rosa26LSL-NICD using a Qiagen Micro Plus kit. cDNA was synthesised using SuperScript III reverse transcriptase (Life Technologies). qRT-PC was performed with Power SYBR Green Master Mix (Life Technologies) and reactions measured in a C1000 Thermal Cycler (Bio-Rad) under the following conditions: 95°C for 5 min, 40 cycles 95°C for 15 s and 60°C for 1 min. Ptch1 , Gli1 , Hes1 ( ) and cHairy2 primers (F: 5′-CCGTACCCTGCAAGCCAGGTG-3′, R: 5′-GCCCATCA-GAGGCAAGCAGCA-3′) were described previously and normalised against β-actin ( ; ) using the Pfaffl equation ( ). .. Image acquisition/analysis Fluorescent signal was acquired using a compound microscope (Leica DM5000 B), an Olympus IX70 deconvolution microscope or the Zeiss LSM-710 confocal microscope.

    Polymerase Chain Reaction:

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Article Title: p63 is an alternative p53 repressor in melanoma that confers chemoresistance and a poor prognosis
    Article Snippet: .. A total of 500 ng RNA was used for using the SuperScript III RT kit (Invitrogen).The primers used for the PCR were as follows: ΔNp63 forward, 5′-GGAAAACAATGCCCAGACTC-3′; and reverse 5′-GAAGGACACGTCGAAACTGTG-3′; TAp63 forward, 5′-GGTGCGACAAACAAGATTGAG-3′; and reverse, 5′-GAAGGACACGTCGAAACTGTG-3′; TP53 forward, 5′-GTCACTGCCATGGAGGAGCCGCA-3′; and reverse, 5′-GACGCACACCTATTGCAAGCAAGGGTTC-3′; GAPDH forward, 5′-CTCCTCCACCTTTGACGCTG-3′; and reverse, 5′-CCACCCTGTTGCTGTAGCCA-3′; and GUS forward, 5′-AAACGATTGCAGGGTTTCAC-3′; and reverse, 5′-CTCTCGTCGGTGACTGTTCA-3′. .. Q-PCR reactions were set up in 96-well plates using Brilliant II SYBR Green QPCR Master Mix (Agilent Technologies).

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

    Article Title: Reprogramming the Dynamin 2 mRNA by Spliceosome-mediated RNA Trans-splicing
    Article Snippet: .. Total RNA (1 µg) was submitted to reverse transcription using the Superscript III reverse transcriptase kit (Life Technologies) using random primers. cDNA were amplified by PCR under the following conditions: 96°C for 5 minutes, cycles of 30 seconds at 96°C, 30 seconds at the appropriate temperature (58 to 61°C), 30 seconds to 1 minute at 72°C, and a final step of 7 minutes at 72°C. ..

    Generated:

    Article Title: Dual transcriptional profiling of mice and Toxoplasma gondii during acute and chronic infection
    Article Snippet: .. RNA was extracted from 5 day bradyzoites grown under low CO2 and high pH conditions using TRIzol. cDNA was generated using the Invitrogen Superscript III Reverse Transcriptase cDNA synthesis kit. ..

    Modification:

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

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    Thermo Fisher t4 dna ligase
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