t4 rna ligase 2 truncated  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    SuperScript III Reverse Transcriptase
    Description:
    Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT that was created by introduction of several mutations for reduced RNase H activity increased half life and improved thermal stability SuperScript III RT offers higher cDNA yields improved cDNA lengths improved efficiency on GC rich target RNAs and overall better performance than wild type MMLV and MMLV RNase H minus enzymes SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    Catalog Number:
    18080044
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher t4 rna ligase 2 truncated
    Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase RT that was created by introduction of several mutations for reduced RNase H activity increased half life and improved thermal stability SuperScript III RT offers higher cDNA yields improved cDNA lengths improved efficiency on GC rich target RNAs and overall better performance than wild type MMLV and MMLV RNase H minus enzymes SuperScript RTs are the most highly trusted and widely used RTs with over 50 000 citations reviews and publications to date Note The latest member of the SuperScript RT family SuperScript IV Reverse Transcriptase features enhanced thermostability processivity yields and performance with any RNA samples including those of suboptimal purity or integrity
    https://www.bioz.com/result/t4 rna ligase 2 truncated/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 2 truncated - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Synthesized:

    Article Title: Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig (Sus scrofa)
    Article Snippet: .. First-strand cDNA was synthesized by reverse transcription (RT) of mRNA using an Oligo(dT)12–18 primer and SuperScript TM III Reverse Transcriptase (Invitrogen Co., Grand Island, NY, USA). .. Real-time RT-polymerase chain reaction (PCR) using the CFX96 Touch real-time RT-PCR Detection System (Bio-Rad, Hercules, CA, USA) was performed in a final reaction volume of 20 μL with SYBR Green, a fluorophore that binds all double-stranded DNA.

    Isolation:

    Article Title: The Mycobacterium tuberculosis relBE toxin:antitoxin genes are stress-responsive modules that regulate growth through translation inhibition
    Article Snippet: .. Reverse transcription reactions were carried out with 4 pmol of labeled primer, 10 μg of LIX32 or LIX33 total RNA (growth and isolation described above), and Superscript III reverse transcriptase (Invitrogen). .. Primer extension reactions were performed using at least two independently isolated RNA samples.

    Labeling:

    Article Title: The Mycobacterium tuberculosis relBE toxin:antitoxin genes are stress-responsive modules that regulate growth through translation inhibition
    Article Snippet: .. Reverse transcription reactions were carried out with 4 pmol of labeled primer, 10 μg of LIX32 or LIX33 total RNA (growth and isolation described above), and Superscript III reverse transcriptase (Invitrogen). .. Primer extension reactions were performed using at least two independently isolated RNA samples.

    Purification:

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

    Real-time Polymerase Chain Reaction:

    Article Title: The tumour-promoting receptor tyrosine kinase, EphB4, regulates expression of Integrin-β8 in prostate cancer cells
    Article Snippet: .. Quantitative real-time PCR Extracted RNA was reverse transcribed using Superscript III reverse transcriptase according to the manufacturer’s instructions (Life Technologies). .. Quantitative RT-PCR for EPHB4 expression was performed in triplicate using a TaqMan Gene Expression Assay (EPHB4 : Hs00174752_m1 Life Technologies) and TaqMan Universal PCR Master Mix, No AmpErase UNG (Life Technologies) on a Rotor-Gene 6000 (Qiagen).

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Deficiency in the nuclear long noncoding RNA Charme causes myogenic defects and heart remodeling in mice
    Article Snippet: .. For semiquantitative and quantitative PCR analyses, RNA (0.5–1.0 μg) was reverse‐transcribed using SuperScript III RT (Life Technologies) according to the manufacturer's instructions and amplified by PCR using MyTaq (Bioline) enzyme. .. Real‐Time PCRs (qPCRs) were performed using QuantiTect SYBR Green (Qiagen).

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

    Generated:

    Article Title: Arabidopsis Histone Reader EMSY-LIKE 1 Binds H3K36 and Suppresses Geminivirus Infection
    Article Snippet: .. For HA- or FLAG-tagged EML1 and EML3, cDNA was generated from plant RNA using SuperScript III RT (ThermoFisher). .. Primers containing the restriction sites PacI and AscI were used to amplify EML1 or EML3 coding sequences (CDSs) using high-fidelity Pfx polymerase (Invitrogen).

    Modification:

    Article Title: The alternative splicing factor Nova2 regulates vascular development and lumen formation
    Article Snippet: .. Total RNA was extracted from 24-hpf pooled embryos with TRIzol reagent (Invitrogen), purified with the RNeasy Mini Kit (QIAGEN) and retro-transcribed with d(T)18 oligo or gene-specific primer and Superscript III RT (Invitrogen) and then analysed in PCR for AS modification of nova2 targets. .. To rescue morphological and vascular defects due to the knockdown of nova2 , we amplified with primers CG30F/CG13R ( ) by using RT generated from Tg(fli1a:EGFP)y1 embryos, a morpholino-resistant zebrafish nova2 cDNA, with six mismatches in the pairing region with the morpholino, which, however, do not alter the amino-acid sequence of the translated protein.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher t4 rna ligase 10 u µl
    In vitro preparation of circular RNA sponges. ( A ) Schematic overview of the ciRS production procedure—starting with in vitro transcription with 10-fold excess of GMP, followed by DNase digestion to remove template DNA, gel filtration to clear away excess nucleotides (NTP, GMP) derived from the transcription reaction, and ligation of the purified transcript. Considering the activity of the <t>T4</t> RNA ligase, transcript ligation can result in both linear monomers and dimers of the transcript and circularized transcripts (indicated by the dash/double dash or the circle in (B) and (C)). ( B ) In vitro circularisation reaction of two exemplary constructs was analysed by 7% polyacrylamide-urea gel electrophoresis, followed by ethidium bromide staining showing linear and circular transcripts with a circularisation efficiency of ~ 40%–60% and only small amounts of linear dimers detectable. As described in Section 3.1 , the transcripts are characterized by a constant region and four miRNA binding sites but differ in the presence of a double-stranded stem-loop region increasing the circularisation efficiency (construct A: 197 nt, lacking the 11 nt 3′stem-loop-sequence; construct B: 208 nt, with stem-loop). ( C ) Circular and linear isoforms of the RNA sponges (ciRS and liRS) were purified, and quality was verified on analytic 6% and 7% polyacrylamide-urea gels by ethidium bromide staining. While the mobility of linear RNAs remains unchanged compared to the RNA ladder, the mobility of circular RNA appears lower in higher percentage polyacrylamide-urea gels, resulting in a shift of the circular RNA when comparing the polyacrylamide-urea gels with different concentrations.
    T4 Rna Ligase 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase 10 u µl/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 10 u µl - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher t4 dna ligase
    In vitro preparation of circular RNA sponges. ( A ) Schematic overview of the ciRS production procedure—starting with in vitro transcription with 10-fold excess of GMP, followed by DNase digestion to remove template DNA, gel filtration to clear away excess nucleotides (NTP, GMP) derived from the transcription reaction, and ligation of the purified transcript. Considering the activity of the <t>T4</t> RNA ligase, transcript ligation can result in both linear monomers and dimers of the transcript and circularized transcripts (indicated by the dash/double dash or the circle in (B) and (C)). ( B ) In vitro circularisation reaction of two exemplary constructs was analysed by 7% polyacrylamide-urea gel electrophoresis, followed by ethidium bromide staining showing linear and circular transcripts with a circularisation efficiency of ~ 40%–60% and only small amounts of linear dimers detectable. As described in Section 3.1 , the transcripts are characterized by a constant region and four miRNA binding sites but differ in the presence of a double-stranded stem-loop region increasing the circularisation efficiency (construct A: 197 nt, lacking the 11 nt 3′stem-loop-sequence; construct B: 208 nt, with stem-loop). ( C ) Circular and linear isoforms of the RNA sponges (ciRS and liRS) were purified, and quality was verified on analytic 6% and 7% polyacrylamide-urea gels by ethidium bromide staining. While the mobility of linear RNAs remains unchanged compared to the RNA ladder, the mobility of circular RNA appears lower in higher percentage polyacrylamide-urea gels, resulting in a shift of the circular RNA when comparing the polyacrylamide-urea gels with different concentrations.
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Thermo Fisher
    Average 99 stars, based on 550 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher t4 dna ligase buffer
    In vitro preparation of circular RNA sponges. ( A ) Schematic overview of the ciRS production procedure—starting with in vitro transcription with 10-fold excess of GMP, followed by DNase digestion to remove template DNA, gel filtration to clear away excess nucleotides (NTP, GMP) derived from the transcription reaction, and ligation of the purified transcript. Considering the activity of the <t>T4</t> RNA ligase, transcript ligation can result in both linear monomers and dimers of the transcript and circularized transcripts (indicated by the dash/double dash or the circle in (B) and (C)). ( B ) In vitro circularisation reaction of two exemplary constructs was analysed by 7% polyacrylamide-urea gel electrophoresis, followed by ethidium bromide staining showing linear and circular transcripts with a circularisation efficiency of ~ 40%–60% and only small amounts of linear dimers detectable. As described in Section 3.1 , the transcripts are characterized by a constant region and four miRNA binding sites but differ in the presence of a double-stranded stem-loop region increasing the circularisation efficiency (construct A: 197 nt, lacking the 11 nt 3′stem-loop-sequence; construct B: 208 nt, with stem-loop). ( C ) Circular and linear isoforms of the RNA sponges (ciRS and liRS) were purified, and quality was verified on analytic 6% and 7% polyacrylamide-urea gels by ethidium bromide staining. While the mobility of linear RNAs remains unchanged compared to the RNA ladder, the mobility of circular RNA appears lower in higher percentage polyacrylamide-urea gels, resulting in a shift of the circular RNA when comparing the polyacrylamide-urea gels with different concentrations.
    T4 Dna Ligase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase buffer/product/Thermo Fisher
    Average 99 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    In vitro preparation of circular RNA sponges. ( A ) Schematic overview of the ciRS production procedure—starting with in vitro transcription with 10-fold excess of GMP, followed by DNase digestion to remove template DNA, gel filtration to clear away excess nucleotides (NTP, GMP) derived from the transcription reaction, and ligation of the purified transcript. Considering the activity of the T4 RNA ligase, transcript ligation can result in both linear monomers and dimers of the transcript and circularized transcripts (indicated by the dash/double dash or the circle in (B) and (C)). ( B ) In vitro circularisation reaction of two exemplary constructs was analysed by 7% polyacrylamide-urea gel electrophoresis, followed by ethidium bromide staining showing linear and circular transcripts with a circularisation efficiency of ~ 40%–60% and only small amounts of linear dimers detectable. As described in Section 3.1 , the transcripts are characterized by a constant region and four miRNA binding sites but differ in the presence of a double-stranded stem-loop region increasing the circularisation efficiency (construct A: 197 nt, lacking the 11 nt 3′stem-loop-sequence; construct B: 208 nt, with stem-loop). ( C ) Circular and linear isoforms of the RNA sponges (ciRS and liRS) were purified, and quality was verified on analytic 6% and 7% polyacrylamide-urea gels by ethidium bromide staining. While the mobility of linear RNAs remains unchanged compared to the RNA ladder, the mobility of circular RNA appears lower in higher percentage polyacrylamide-urea gels, resulting in a shift of the circular RNA when comparing the polyacrylamide-urea gels with different concentrations.

    Journal: Methods and Protocols

    Article Title: Production and Purification of Artificial Circular RNA Sponges for Application in Molecular Biology and Medicine

    doi: 10.3390/mps3020042

    Figure Lengend Snippet: In vitro preparation of circular RNA sponges. ( A ) Schematic overview of the ciRS production procedure—starting with in vitro transcription with 10-fold excess of GMP, followed by DNase digestion to remove template DNA, gel filtration to clear away excess nucleotides (NTP, GMP) derived from the transcription reaction, and ligation of the purified transcript. Considering the activity of the T4 RNA ligase, transcript ligation can result in both linear monomers and dimers of the transcript and circularized transcripts (indicated by the dash/double dash or the circle in (B) and (C)). ( B ) In vitro circularisation reaction of two exemplary constructs was analysed by 7% polyacrylamide-urea gel electrophoresis, followed by ethidium bromide staining showing linear and circular transcripts with a circularisation efficiency of ~ 40%–60% and only small amounts of linear dimers detectable. As described in Section 3.1 , the transcripts are characterized by a constant region and four miRNA binding sites but differ in the presence of a double-stranded stem-loop region increasing the circularisation efficiency (construct A: 197 nt, lacking the 11 nt 3′stem-loop-sequence; construct B: 208 nt, with stem-loop). ( C ) Circular and linear isoforms of the RNA sponges (ciRS and liRS) were purified, and quality was verified on analytic 6% and 7% polyacrylamide-urea gels by ethidium bromide staining. While the mobility of linear RNAs remains unchanged compared to the RNA ladder, the mobility of circular RNA appears lower in higher percentage polyacrylamide-urea gels, resulting in a shift of the circular RNA when comparing the polyacrylamide-urea gels with different concentrations.

    Article Snippet: T4 RNA Ligase (Thermo Fisher Scientific, Waltham, MA, USA; Cat. no.: EL0021).

    Techniques: In Vitro, Filtration, Derivative Assay, Ligation, Purification, Activity Assay, Construct, Nucleic Acid Electrophoresis, Staining, Binding Assay, Sequencing