t4 rna ligase 1  (New England Biolabs)


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    Structured Review

    New England Biolabs t4 rna ligase 1
    T4 Rna Ligase 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase 1/product/New England Biolabs
    Average 99 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 1 - by Bioz Stars, 2020-01
    99/100 stars

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    Incubation:

    Article Title: Interpreting reverse transcriptase termination and mutation events for greater insight into the chemical probing of RNA
    Article Snippet: .. Ligation mixture was incubated at 65°C for 2 h, then 85°C for 15 min. For ligation of 3′ adaptor using T4 RNA ligase 1, 2 μl 10× T4 RNA ligase buffer, 10 μl 50% PEG 8000, 1 μl 1 mM ATP, 1 μl 20 μM 3′ adaptor, 1 μl T4 RNA ligase 1 (NEB), 5 μl cDNA. ..

    Amplification:

    Article Title: Interpreting reverse transcriptase termination and mutation events for greater insight into the chemical probing of RNA
    Article Snippet: Ligation mixture was incubated at 65°C for 2 h, then 85°C for 15 min. For ligation of 3′ adaptor using T4 RNA ligase 1, 2 μl 10× T4 RNA ligase buffer, 10 μl 50% PEG 8000, 1 μl 1 mM ATP, 1 μl 20 μM 3′ adaptor, 1 μl T4 RNA ligase 1 (NEB), 5 μl cDNA. .. Products were amplified for 4 cycles with Phusion (New England Biolabs) polymerase using primers matching the 5′ and 3′ adaptor sequences (5′-CAGACGTGTGCTCTTCCGATC-3′; 5′-CTACACGACGCTCTTCCGATCT-3′), with cycles of 98 °C, 20 s; 64 °C, 20 s; 72 °C, 90 s. Products were purified as before with 1 volume AMPure XP beads.

    Ligation:

    Article Title: Interpreting reverse transcriptase termination and mutation events for greater insight into the chemical probing of RNA
    Article Snippet: .. Ligation mixture was incubated at 65°C for 2 h, then 85°C for 15 min. For ligation of 3′ adaptor using T4 RNA ligase 1, 2 μl 10× T4 RNA ligase buffer, 10 μl 50% PEG 8000, 1 μl 1 mM ATP, 1 μl 20 μM 3′ adaptor, 1 μl T4 RNA ligase 1 (NEB), 5 μl cDNA. ..

    Purification:

    Article Title: Interpreting reverse transcriptase termination and mutation events for greater insight into the chemical probing of RNA
    Article Snippet: Reverse transcription reaction cDNA products were purified with AMPure XP (Beckman Coulter) beads by adding 1 volume beads solution (at room temperature), 3 volumes PEG solution (2.5 M NaCl, 20% PEG 8000), and 1 volume iPrOH, mixed and allowed to sit at room temperature for 15 min. Beads were immobilized for 10 min on a magnetic rack, then washed twice with fresh 80% EtOH, allowed to dry for 10 minutes, then eluted in 16 μl dH 2O. .. Ligation mixture was incubated at 65°C for 2 h, then 85°C for 15 min. For ligation of 3′ adaptor using T4 RNA ligase 1, 2 μl 10× T4 RNA ligase buffer, 10 μl 50% PEG 8000, 1 μl 1 mM ATP, 1 μl 20 μM 3′ adaptor, 1 μl T4 RNA ligase 1 (NEB), 5 μl cDNA.

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  • 90
    New England Biolabs t4 rna ligase 1
    T4 Rna Ligase 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase 1/product/New England Biolabs
    Average 90 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase 1 - by Bioz Stars, 2020-01
    90/100 stars
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