t4 polynucleotide kinase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs t4 polynucleotide kinase
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
    Average 99 stars, based on 377 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Mutagenesis:

    Article Title: Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivo
    Article Snippet: In this protocol, we have used the XPA-complemented (GM15876A) and XPA-deficient (XP12RO) human fibroblast cell lines as an example to illustrate the application of the CTAB method for transcriptional lesion bypass, mutagenesis and repair studies in viv o. .. Cycle Pure kit (Omega Bio-Tek, cat. no. D6492-02) QIAprep spin miniprep kit (Qiagen, cat. no.27104) EcoRI (New England BioLabs, cat. no. R0101S) NheI (New England BioLabs, cat. no. R0131S) Shrimp alkaline phosphatase (New England BioLabs, cat. no. R0371S) Adenosine 5′-triphosphate (ATP; New England BioLabs, cat. no. R0756S) T4 polynucleotide kinase (T4 PNK; New England BioLabs, cat. no. R0201S) T4 DNA ligase (New England BioLabs, cat. no. R0202L) Nt.BstNBI (New England BioLabs, cat. no. R0607L) Ethidium bromide (Sigma-Aldrich, cat. no. E1510) !

    Hood:

    Article Title: Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivo
    Article Snippet: Cycle Pure kit (Omega Bio-Tek, cat. no. D6492-02) QIAprep spin miniprep kit (Qiagen, cat. no.27104) EcoRI (New England BioLabs, cat. no. R0101S) NheI (New England BioLabs, cat. no. R0131S) Shrimp alkaline phosphatase (New England BioLabs, cat. no. R0371S) Adenosine 5′-triphosphate (ATP; New England BioLabs, cat. no. R0756S) T4 polynucleotide kinase (T4 PNK; New England BioLabs, cat. no. R0201S) T4 DNA ligase (New England BioLabs, cat. no. R0202L) Nt.BstNBI (New England BioLabs, cat. no. R0607L) Ethidium bromide (Sigma-Aldrich, cat. no. E1510) ! .. CAUTION Phenol:chloroform:isoamyl alcohol is toxic and corrosive; wear gloves and work in a fume hood.

    Synthesized:

    Article Title: Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivo
    Article Snippet: In this protocol, the 12-mer lesion-containing ODNs (5′-ATGGCGXGCTAT-3′, X = N 3-CMdT or O 4 -CMdT) were previously synthesized in our laboratory Appropriate cell lines. .. Cycle Pure kit (Omega Bio-Tek, cat. no. D6492-02) QIAprep spin miniprep kit (Qiagen, cat. no.27104) EcoRI (New England BioLabs, cat. no. R0101S) NheI (New England BioLabs, cat. no. R0131S) Shrimp alkaline phosphatase (New England BioLabs, cat. no. R0371S) Adenosine 5′-triphosphate (ATP; New England BioLabs, cat. no. R0756S) T4 polynucleotide kinase (T4 PNK; New England BioLabs, cat. no. R0201S) T4 DNA ligase (New England BioLabs, cat. no. R0202L) Nt.BstNBI (New England BioLabs, cat. no. R0607L) Ethidium bromide (Sigma-Aldrich, cat. no. E1510) !

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    New England Biolabs t4 polynucleotide kinase
    Removal of the terminal 2′,3′ cyclic phosphate group of the released tRNA resulting from the HDV ribozyme cleavage reaction. Treatment with <t>T4</t> polynucleotide kinase (T4 PNK) leads to the removal of the phosphate group and therefore to a reduced net charge of the RNA. This can be observed by a reduced electrophoretic mobility on a denaturing 10% polyacrylamide gel in comparison with the untreated control (mock incubation without T4 PNK).
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
    Average 90 stars, based on 377 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    Removal of the terminal 2′,3′ cyclic phosphate group of the released tRNA resulting from the HDV ribozyme cleavage reaction. Treatment with T4 polynucleotide kinase (T4 PNK) leads to the removal of the phosphate group and therefore to a reduced net charge of the RNA. This can be observed by a reduced electrophoretic mobility on a denaturing 10% polyacrylamide gel in comparison with the untreated control (mock incubation without T4 PNK).

    Journal: Nucleic Acids Research

    Article Title: A universal method to produce in vitro transcripts with homogeneous 3? ends

    doi:

    Figure Lengend Snippet: Removal of the terminal 2′,3′ cyclic phosphate group of the released tRNA resulting from the HDV ribozyme cleavage reaction. Treatment with T4 polynucleotide kinase (T4 PNK) leads to the removal of the phosphate group and therefore to a reduced net charge of the RNA. This can be observed by a reduced electrophoretic mobility on a denaturing 10% polyacrylamide gel in comparison with the untreated control (mock incubation without T4 PNK).

    Article Snippet: Radioactively labeled transcripts carrying a 2′,3′ cyclic phosphate at the 3′ end (as a consequence of the self-cleavage reaction of the downstream HDV ribozyme) were dephosphorylated using either of the following methods. (i) Up to 50 pmol RNA were incubated with 6 U T4 polynucleotide kinase (New England Biolabs) in 100 mM Tris–HCl pH 6.5, 100 mM magnesium acetate, 5 mM β-mercaptoethanol in a final volume of 50 µl for 6 h at 37°C ( ). (ii) Transcripts were incubated with 0.1 mM ATP, 100 mM imidazole–HCl pH 6.0, 10 mM MgCl2 , 10 mM β-mercaptoethanol, 20 µg/ml BSA and 1 U T4 polynucleotide kinase (New England Biolabs) per 100 pmol RNA in a final volume of 50 µl for 6 h at 37°C ( ). (iii) Up to 300 pmol tRNA were incubated in 100 mM morpholinoethanesulfonate-NaOH pH 5.5, 10 mM MgCl2 , 10 mM β-mercaptoethanol, 300 mM NaCl and 10 U T4 polynucleotide kinase (New England Biolabs) in a final volume of 20 µl for 6 h at 37°C ( ).

    Techniques: Incubation