t4 ligase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher t4 ligase
    T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Thermo Fisher
    Average 99 stars, based on 181 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: Paragraph title: Cloning of Gal-1 ... The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA). .. The constructs were screened using PCR with Taq polymerase and the sequences of the constructs were verified by DNA sequencing (RuibioBiotech, Beijing, China).

    Article Title: Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog
    Article Snippet: The 3.6kb PCR product was gel extracted, digested with SapI, re-circularised using T4 ligase and electroporated into MegaX DH10B (Invitrogen) cells to afford a library with a diversity of ≈5.1×107 colony forming units (c.f.u), surpassing the required theoretical diversity of 4.8×106 c.f.u. .. The pool of DNA clones was subsequently extracted by DNA maxiprep and frozen.

    Centrifugation:

    Article Title: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics
    Article Snippet: The ligation mixture was concentrated and purified using Amicon Ultra 30 K centrifugation units (Millipore, Merck, USA). .. 5 μg of the DNA was circularized using T4 ligase with 45 U of T4 ligase (Thermo Scientific, USA), 10% v/v of PEG 4000 (Thermo Scientific, USA) and in a final volume of 5 ml of 1X T4 ligation buffer.

    Amplification:

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol. .. A bacterial artificial chromosome (BAC) clone that covered the genome segment of the target regions and that was applied to eliminate amplification efficiency differences among different primers was treated with the same procedures.

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: .. The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA). .. A hybridization mix consisting of 100 mM Tris-HCl (pH 8), 100 mM EDTA, 0.5% Tween-20, 2.5 M NaCl was added to the RCA coil solution.

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: The cDNA of hSSTR1 and hSSTR4 was amplified with PrimeSTAR using the polymerase chain reaction (PCR) with the following primers: hSSTR1 (1,176 bp), sense, 5′-CCGGAATTCGCCACCATGTTCCCCAATGGCACCG-3′, and antisense, 5′-CCGCTCGAGTCAGAGCGTCGTGATCCGG-3′; and hSSTR4 (1,167 bp), sense, 5′-CCGGAATTCGCCACCATGAGCGCCCCCTCGACG-3′, and antisense, 5′-CCGCTCGAGTCAGAAGGTGGTGGTCCTGG-3′. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: .. The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA). .. A hybridization mix consisting of 100 mM Tris-HCl (pH 8), 100 mM EDTA, 0.5% Tween-20, 2.5 M NaCl was added to the RCA coil solution.

    Reporter Assay:

    Article Title: DNA methylome and transcriptome alterations and cancer prevention by curcumin in colitis-accelerated colon cancer in mice
    Article Snippet: Paragraph title: Plasmids, transfection and luciferase reporter assay ... The restriction enzymes HindIII and NcoI, T4 ligase and CpG methyltransferase M.SssI were purchased from Thermofisher.

    Synthesized:

    Article Title: Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode
    Article Snippet: T4 ligase, inorganic pyrophosphatase, protein ladder (#26614) and restriction endonucleases were obtained from Thermo Scientific (Waltham, MA, USA). .. Oligonucleotide primers were synthesized by Life Technologies (Carlsbad, CA, USA).

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA). .. Briefly, 2 forward primers containing AP4 binding sites to Gal-1 promoter sequences from −31 to +4 were synthesized.

    Construct:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: The 5′ flanking DNA of Gal-1 gene containing the predicting binding sites of AP4 were constructed with PCR that generated a 2512-bp-long DNA fragment. .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: Paragraph title: Expression constructs of hSSTRs ... The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: The library was constructed by performing a genomic DNA prep of H131E-A31 using the Wizard Genomic DNA purification kit (Promega) and partially digesting the DNA with Sau3AI (New England Biolabs). .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    Real-time Polymerase Chain Reaction:

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol. .. Physical interactions among anchor and test primers were measured by qPCR.

    Incubation:

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: The MNPs were thereafter resuspended in 1× Wtw buffer and incubated with 10 µM biotin-conjugated oligonucleotides for 30 min at room temperature. .. The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: Both the insertion fragments and the backbone were incubated with the Klenow fragment of DNA polymerase I and the appropriate dNTPs to reduce the overhang length from 4 to 2 base pairs to decrease the frequency of self-ligation. .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: The MNPs were thereafter resuspended in 1× Wtw buffer and incubated with 10 µM biotin-conjugated oligonucleotides for 30 min at room temperature. .. The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA).

    Luciferase:

    Article Title: DNA methylome and transcriptome alterations and cancer prevention by curcumin in colitis-accelerated colon cancer in mice
    Article Snippet: Paragraph title: Plasmids, transfection and luciferase reporter assay ... The restriction enzymes HindIII and NcoI, T4 ligase and CpG methyltransferase M.SssI were purchased from Thermofisher.

    Expressing:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: Paragraph title: Expression constructs of hSSTRs ... The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Transformation Assay:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA). .. Briefly, 2 forward primers containing AP4 binding sites to Gal-1 promoter sequences from −31 to +4 were synthesized.

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes. ..

    Hybridization:

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA). .. A hybridization mix consisting of 100 mM Tris-HCl (pH 8), 100 mM EDTA, 0.5% Tween-20, 2.5 M NaCl was added to the RCA coil solution.

    Article Title: Development of a Sensitive Induction-Based Magnetic Nanoparticle Biodetection Method
    Article Snippet: The RCA process was conducted as follows: 5 nM of padlock probes (Biomers, Ulm, Germany) were hybridized and ligated, templated by 15 nM synthetic Vibrio cholerae target DNA in a solution consisting of 1× Phi29 buffer, 0.25 mM ATP and 5 mU/μL T4 ligase at 37 °C for 15 min. Circular padlock probes were amplified in 1× Phi29 buffer, 0.1 mM dNTP, 0.12 μg/μL BSA, 4 mU/μL Phi29 polymerase at 37 °C for 1 h, followed by enzymatic inactivation at 65 °C for 5 min (Phi29 buffer, ATP, T4 ligase, dNTP, BSA and Phi29 polymerase from Thermo Fisher Scientific, Waltham, MA, USA). .. A hybridization mix consisting of 100 mM Tris-HCl (pH 8), 100 mM EDTA, 0.5% Tween-20, 2.5 M NaCl was added to the RCA coil solution.

    Transfection:

    Article Title: DNA methylome and transcriptome alterations and cancer prevention by curcumin in colitis-accelerated colon cancer in mice
    Article Snippet: Paragraph title: Plasmids, transfection and luciferase reporter assay ... The restriction enzymes HindIII and NcoI, T4 ligase and CpG methyltransferase M.SssI were purchased from Thermofisher.

    Inverse PCR:

    Article Title: Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog
    Article Snippet: To create the pSertRNA(10)CUA library, the 5 bases either side of the anticodon of pSer tRNACUA in pSertRNACUA were randomised by Enzymatic Inverse PCR (EI-PCR) using KOD polymerase (Novagen) and primers DTR195/DTR198. .. The 3.6kb PCR product was gel extracted, digested with SapI, re-circularised using T4 ligase and electroporated into MegaX DH10B (Invitrogen) cells to afford a library with a diversity of ≈5.1×107 colony forming units (c.f.u), surpassing the required theoretical diversity of 4.8×106 c.f.u.

    Ligation:

    Article Title: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics
    Article Snippet: .. 5 μg of the DNA was circularized using T4 ligase with 45 U of T4 ligase (Thermo Scientific, USA), 10% v/v of PEG 4000 (Thermo Scientific, USA) and in a final volume of 5 ml of 1X T4 ligation buffer. .. The ligated DNA was concentrated and purified using AMPure XP SPRI paramagnetic beads (Agencourt, Beckman Coulter Inc., USA) according to manufacturer’s instructions and eluted in sterile MilliQ H2O.

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: .. Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol. .. A bacterial artificial chromosome (BAC) clone that covered the genome segment of the target regions and that was applied to eliminate amplification efficiency differences among different primers was treated with the same procedures.

    Protease Inhibitor:

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: Cells were fixed with formaldehyde, which was stopped with glycine, and lysed in lysis buffer (10 mM Tris-HCl [pH 7.5]; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; and 1× complete protease inhibitor; 11836145001 Roche), then digested with Mbo-I enzyme (New England Biolabs) at 37°C overnight. .. Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol.

    Cell Culture:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: Total mRNA was extracted from cultured HeLa cells using TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) reagent, according to the manufacturer's protocol. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Generated:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: The 5′ flanking DNA of Gal-1 gene containing the predicting binding sites of AP4 were constructed with PCR that generated a 2512-bp-long DNA fragment. .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA).

    DNA Sequencing:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA). .. The constructs were screened using PCR with Taq polymerase and the sequences of the constructs were verified by DNA sequencing (RuibioBiotech, Beijing, China).

    Polymerase Chain Reaction:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA). .. Briefly, 2 forward primers containing AP4 binding sites to Gal-1 promoter sequences from −31 to +4 were synthesized.

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA). .. The constructs were screened using PCR with Taq polymerase and the sequences of the constructs were verified by DNA sequencing (RuibioBiotech, Beijing, China).

    Article Title: Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog
    Article Snippet: .. The 3.6kb PCR product was gel extracted, digested with SapI, re-circularised using T4 ligase and electroporated into MegaX DH10B (Invitrogen) cells to afford a library with a diversity of ≈5.1×107 colony forming units (c.f.u), surpassing the required theoretical diversity of 4.8×106 c.f.u. .. The pool of DNA clones was subsequently extracted by DNA maxiprep and frozen.

    Binding Assay:

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: The 5′ flanking DNA of Gal-1 gene containing the predicting binding sites of AP4 were constructed with PCR that generated a 2512-bp-long DNA fragment. .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA).

    DNA Extraction:

    Article Title: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics
    Article Snippet: Paragraph title: DNA isolation, restriction enzyme digestion and circularization ... 5 μg of the DNA was circularized using T4 ligase with 45 U of T4 ligase (Thermo Scientific, USA), 10% v/v of PEG 4000 (Thermo Scientific, USA) and in a final volume of 5 ml of 1X T4 ligation buffer.

    BAC Assay:

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol. .. A bacterial artificial chromosome (BAC) clone that covered the genome segment of the target regions and that was applied to eliminate amplification efficiency differences among different primers was treated with the same procedures.

    Mutagenesis:

    Article Title: Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode
    Article Snippet: The KOD-plus mutagenesis kit was obtained from TOYOBO (Japan). .. T4 ligase, inorganic pyrophosphatase, protein ladder (#26614) and restriction endonucleases were obtained from Thermo Scientific (Waltham, MA, USA).

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA). .. Primers 5′‑TCTGTCAGCTATTTTA TTTTT CAAT‑3′ and 5′‑AGACAGTCGATAAAAT AAAAA GTTA‑3′ were used for the mutagenesis of the Gal-1 promoter. (Nucleotide bases in italics refer to the predicted binding sites of AP4, and those in bold are the mutation sites.)

    Article Title: Translational fidelity maintenance preventing Ser mis-incorporation at Thr codon in protein from eukaryote
    Article Snippet: KOD-plus mutagenesis kit was obtained from TOYOBO (Japan). .. T4 ligase and restriction endonucleases were obtained from MBI Fermentas.

    Size-exclusion Chromatography:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: PCR was performed in a Peltier Thermal Cycler 200 programmed with an initial denaturation at 98°C for 5 min followed by 35 cycles of denaturation at 98°C for 30 sec, annealing at 55–58°C for 30 sec and extension at 72°C for 90 sec. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Purification:

    Article Title: Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode
    Article Snippet: The Pfu DNA polymerase, the DNA fragment rapid purification kit and plasmid extraction kit were purchased from Tiangen Biotech (China). .. T4 ligase, inorganic pyrophosphatase, protein ladder (#26614) and restriction endonucleases were obtained from Thermo Scientific (Waltham, MA, USA).

    Article Title: In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity
    Article Snippet: Materials T4 polynucleotide kinase (PNK), T4 ligase and FastAPTM were purchased from MBI Fermentas. .. All reagents and chemicals, including H2 O18 , NaN3 , DABCO, DMSO, H2 O2 , DTT and t -BuOH were analytical grade and used without further purification.

    Article Title: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics
    Article Snippet: The ligation mixture was concentrated and purified using Amicon Ultra 30 K centrifugation units (Millipore, Merck, USA). .. 5 μg of the DNA was circularized using T4 ligase with 45 U of T4 ligase (Thermo Scientific, USA), 10% v/v of PEG 4000 (Thermo Scientific, USA) and in a final volume of 5 ml of 1X T4 ligation buffer.

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA). .. The constructs were screened using PCR with Taq polymerase and the sequences of the constructs were verified by DNA sequencing (RuibioBiotech, Beijing, China).

    Article Title: Translational fidelity maintenance preventing Ser mis-incorporation at Thr codon in protein from eukaryote
    Article Snippet: [3 H]Thr was obtained from Biotrend chemicals (Destin, USA), [14 C]Ser and [α-32 P]ATP were obtained from Perkin Elmer Inc. Pfu DNA polymerase, the DNA fragment rapid purification kit and a plasmid extraction kit were purchased from Biotech Company (China). .. T4 ligase and restriction endonucleases were obtained from MBI Fermentas.

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: Fragments larger than 3 kb were selected on an agarose gel and the DNA was gel purified and purified again with ethanol precipitation. pRS415 was used as the backbone and digested with SalI. .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    Polyacrylamide Gel Electrophoresis:

    Article Title: In vitro selection of DNA-cleaving deoxyribozyme with site-specific thymidine excision activity
    Article Snippet: Materials T4 polynucleotide kinase (PNK), T4 ligase and FastAPTM were purchased from MBI Fermentas. .. ATP, dCTP and all oligonucleotides were PAGE-purified by Sangon Biotech (Shanghai, China).

    Lysis:

    Article Title: Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction
    Article Snippet: Cells were fixed with formaldehyde, which was stopped with glycine, and lysed in lysis buffer (10 mM Tris-HCl [pH 7.5]; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; and 1× complete protease inhibitor; 11836145001 Roche), then digested with Mbo-I enzyme (New England Biolabs) at 37°C overnight. .. Ligation was performed with T4 ligase (Thermo Fisher Scientific) at 16°C for 6 h. The cross-linked DNA fragments were extracted by phenol/chloroform and precipitated with ethanol.

    Plasmid Preparation:

    Article Title: Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode
    Article Snippet: The Pfu DNA polymerase, the DNA fragment rapid purification kit and plasmid extraction kit were purchased from Tiangen Biotech (China). .. T4 ligase, inorganic pyrophosphatase, protein ladder (#26614) and restriction endonucleases were obtained from Thermo Scientific (Waltham, MA, USA).

    Article Title: CRISPR/Cas9-mediated gene targeting during embryogenesis in swine
    Article Snippet: pX330 vector (Addgene). .. T4 ligase and buffer. mMESSAGE mMACHINE® T7 Ultra Kit (Ambion).

    Article Title: Galectin-1 is a new fibrosis protein in type 1 and type 2 diabetes
    Article Snippet: .. The PCR products were digested with Eco RV and ligated into pGL4.1 basic vector using T4 ligase followed by transformation of DH5α cells (Thermo Fisher Scientific, Waltham, MA, USA). .. Briefly, 2 forward primers containing AP4 binding sites to Gal-1 promoter sequences from −31 to +4 were synthesized.

    Article Title: Translational fidelity maintenance preventing Ser mis-incorporation at Thr codon in protein from eukaryote
    Article Snippet: [3 H]Thr was obtained from Biotrend chemicals (Destin, USA), [14 C]Ser and [α-32 P]ATP were obtained from Perkin Elmer Inc. Pfu DNA polymerase, the DNA fragment rapid purification kit and a plasmid extraction kit were purchased from Biotech Company (China). .. T4 ligase and restriction endonucleases were obtained from MBI Fermentas.

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes. ..

    Electrophoresis:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: The PCR products were verified by electrophoresis on 1% agarose gel and visualized by ethidium bromide staining under ultraviolet light. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Selection:

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes. .. The resulting yeast library was grown on glucose-containing agar plates and the appropriate amino acid dropouts for selection and 5 × 105 colonies formed.

    Agarose Gel Electrophoresis:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: The PCR products were verified by electrophoresis on 1% agarose gel and visualized by ethidium bromide staining under ultraviolet light. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: Fragments larger than 3 kb were selected on an agarose gel and the DNA was gel purified and purified again with ethanol precipitation. pRS415 was used as the backbone and digested with SalI. .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    Ethanol Precipitation:

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: Fragments larger than 3 kb were selected on an agarose gel and the DNA was gel purified and purified again with ethanol precipitation. pRS415 was used as the backbone and digested with SalI. .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    DNA Purification:

    Article Title: Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption
    Article Snippet: The library was constructed by performing a genomic DNA prep of H131E-A31 using the Wizard Genomic DNA purification kit (Promega) and partially digesting the DNA with Sau3AI (New England Biolabs). .. After ligating the fragments and the backbone together with T4 ligase, the resulting plasmid was transformed into ElectroMAX DH5α-E (Invitrogen) and plated on ampicillin-resistant agar Petri dishes.

    Staining:

    Article Title: Expression and selective activation of somatostatin receptor subtypes induces cell cycle arrest in cancer cells
    Article Snippet: The PCR products were verified by electrophoresis on 1% agarose gel and visualized by ethidium bromide staining under ultraviolet light. .. The purified PCR products were then digested with Eco RI/ Xho I and cloned using T4 ligase into pcDNA6-Myc/His (Invitrogen; Thermo Fisher Scientific, Inc.) and pCMV-HA (Clontech Laboratories, Inc., Mountainview, CA, USA).

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  • 90
    Thermo Fisher t4 rna ligase
    T4 Rna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase/product/Thermo Fisher
    Average 90 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher t4 dna ligase
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Thermo Fisher
    Average 90 stars, based on 1134 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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