t4 ligase  (TaKaRa)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    DNA Ligation Kit
    Description:
    DNA Ligation Kit Version 1 and DNA Ligation Kit Version 2 1 enable high efficiency ligation of DNA molecules in vitro Using an optimized buffer system and T4 DNA Ligase these DNA ligation kits offer fast ligation in 30 minutes Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification
    Catalog Number:
    6021
    Price:
    None
    Size:
    50 Rxns
    Category:
    DNA ligation kits quick Ligation kits Cloning
    Buy from Supplier


    Structured Review

    TaKaRa t4 ligase
    DNA Ligation Kit Version 1 and DNA Ligation Kit Version 2 1 enable high efficiency ligation of DNA molecules in vitro Using an optimized buffer system and T4 DNA Ligase these DNA ligation kits offer fast ligation in 30 minutes Ligation reactions can be used directly in selected bacterial transformations with no need for DNA purification
    https://www.bioz.com/result/t4 ligase/product/TaKaRa
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
    Article Snippet: .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A). .. Escherichia coli JM109 cells (TaKaRa Biotech Co.

    Amplification:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Article Title: CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection
    Article Snippet: .. The customised donor template was modified with restriction enzymes from Roche Diagnostics Ltd., Phusion high-fidelity PCR kit (catalogue number F553S, Life Technologies Ltd.) and Mighty Mix DNA Ligation Kit (catalogue number 6023, Takara Bio Europe SAS) to change the puromycin resistance gene to a blasticidin resistance gene amplified from the PSF-CMV-BLAST plasmid (catalogue number OGS588, Sigma-Aldrich Company Ltd.) to create the second donor DNA plasmid. .. Establishment of antibiotic selection conditions ARPE-19 cells and iPSCs were cultured with a range of puromycin (catalogue number ant-pr-1, InvivoGen) or blasticidin (catalogue number ant-bl-1, InvivoGen) concentration for 7 days.

    Agarose Gel Electrophoresis:

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
    Article Snippet: .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A). .. Escherichia coli JM109 cells (TaKaRa Biotech Co.

    DNA Ligation:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Article Title: Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315
    Article Snippet: .. Phosphatase-treated linearized pACYC184 (0.3 μg) and partially Sau 3AI-digested or completely Hin dIII-digested S. aureus N315 DNAs (1.1 to 1.2 μg) were mixed and ligated by using a DNA ligation kit (Takara Shuzo Co. Ltd., Kyoto, Japan). .. The ligated DNAs were transformed into E. coli MC1061, and transformants were selected with antibiotic resistance by plating on L agar plates containing chloramphenicol.

    Article Title: CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection
    Article Snippet: .. The customised donor template was modified with restriction enzymes from Roche Diagnostics Ltd., Phusion high-fidelity PCR kit (catalogue number F553S, Life Technologies Ltd.) and Mighty Mix DNA Ligation Kit (catalogue number 6023, Takara Bio Europe SAS) to change the puromycin resistance gene to a blasticidin resistance gene amplified from the PSF-CMV-BLAST plasmid (catalogue number OGS588, Sigma-Aldrich Company Ltd.) to create the second donor DNA plasmid. .. Establishment of antibiotic selection conditions ARPE-19 cells and iPSCs were cultured with a range of puromycin (catalogue number ant-pr-1, InvivoGen) or blasticidin (catalogue number ant-bl-1, InvivoGen) concentration for 7 days.

    Article Title: PhiC31-based Site-Specific Transgenesis System for Production of Transgenic Bovine Embryos by Somatic Cell Nuclear Transfer and Intracytoplasmic Sperm Injection
    Article Snippet: .. Finally, linearzied and blunted pET15b backbone and phiC31 open reading frame were gel extracted and ligated using DNA Ligation Kit (Takara, Japan). .. The pET-phiC31 expression plasmid was amplified in a DH5α strain of E. coli. (Invitrogen, USA).

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
    Article Snippet: .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A). .. Escherichia coli JM109 cells (TaKaRa Biotech Co.

    Article Title: The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development
    Article Snippet: .. The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023). .. ES cell targeting Vectors were amplified by transformation into Stbl3 competent cells (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Article Title: Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot-blot hybridization
    Article Snippet: .. Both types of PCR products were purified using the TaKaRa DNA Fragment Purification Kit (TaKaRa Bio, Inc.). .. DIG-labeled cDNA probes were prepared using the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Applied Science, Indianapolis, IN, USA) according to the manufacturer’s instructions.

    Article Title: CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection
    Article Snippet: .. The customised donor template was modified with restriction enzymes from Roche Diagnostics Ltd., Phusion high-fidelity PCR kit (catalogue number F553S, Life Technologies Ltd.) and Mighty Mix DNA Ligation Kit (catalogue number 6023, Takara Bio Europe SAS) to change the puromycin resistance gene to a blasticidin resistance gene amplified from the PSF-CMV-BLAST plasmid (catalogue number OGS588, Sigma-Aldrich Company Ltd.) to create the second donor DNA plasmid. .. Establishment of antibiotic selection conditions ARPE-19 cells and iPSCs were cultured with a range of puromycin (catalogue number ant-pr-1, InvivoGen) or blasticidin (catalogue number ant-bl-1, InvivoGen) concentration for 7 days.

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
    Article Snippet: .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A). .. Escherichia coli JM109 cells (TaKaRa Biotech Co.

    Construct:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Purification:

    Article Title: Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot-blot hybridization
    Article Snippet: .. Both types of PCR products were purified using the TaKaRa DNA Fragment Purification Kit (TaKaRa Bio, Inc.). .. DIG-labeled cDNA probes were prepared using the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Applied Science, Indianapolis, IN, USA) according to the manufacturer’s instructions.

    Modification:

    Article Title: CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection
    Article Snippet: .. The customised donor template was modified with restriction enzymes from Roche Diagnostics Ltd., Phusion high-fidelity PCR kit (catalogue number F553S, Life Technologies Ltd.) and Mighty Mix DNA Ligation Kit (catalogue number 6023, Takara Bio Europe SAS) to change the puromycin resistance gene to a blasticidin resistance gene amplified from the PSF-CMV-BLAST plasmid (catalogue number OGS588, Sigma-Aldrich Company Ltd.) to create the second donor DNA plasmid. .. Establishment of antibiotic selection conditions ARPE-19 cells and iPSCs were cultured with a range of puromycin (catalogue number ant-pr-1, InvivoGen) or blasticidin (catalogue number ant-bl-1, InvivoGen) concentration for 7 days.

    DNA Purification:

    Article Title: Expression of recombinant allergen, Der f 1, Der f 2 and Der f 4 using baculovirus-insect cell systems
    Article Snippet: .. After the PCR-amplified DNA was recovered with a MiniBEST Agarose Gel DNA Purification Kit Ver 2.0 (TaKaRa Code No. D823A), it was then cloned into pFastBacHT A (Invitrogen) with a solution of the DNA Ligation Kit (TaKaRa Code No. D6020A). .. Escherichia coli JM109 cells (TaKaRa Biotech Co.

    Sequencing:

    Article Title: The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development
    Article Snippet: .. The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023). .. ES cell targeting Vectors were amplified by transformation into Stbl3 competent cells (Invitrogen).

    Plasmid Preparation:

    Article Title: Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus
    Article Snippet: .. The pVenus vector was used as the backbone to construct the IL2RG -targeting vector pVenus-L. A 5662 bp 5′ homology arm and a 3000 bp 3′ homology arm were amplified by PCR from the Jurkat cell genome, and cloned into the pVenus by using a DNA Ligation Kit. .. Donor DNA was linearized by Eam 1105I or Kpn I digestion and purified by gel electrophoresis.

    Article Title: CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection
    Article Snippet: .. The customised donor template was modified with restriction enzymes from Roche Diagnostics Ltd., Phusion high-fidelity PCR kit (catalogue number F553S, Life Technologies Ltd.) and Mighty Mix DNA Ligation Kit (catalogue number 6023, Takara Bio Europe SAS) to change the puromycin resistance gene to a blasticidin resistance gene amplified from the PSF-CMV-BLAST plasmid (catalogue number OGS588, Sigma-Aldrich Company Ltd.) to create the second donor DNA plasmid. .. Establishment of antibiotic selection conditions ARPE-19 cells and iPSCs were cultured with a range of puromycin (catalogue number ant-pr-1, InvivoGen) or blasticidin (catalogue number ant-bl-1, InvivoGen) concentration for 7 days.

    Article Title: The deubiquitinase Usp9x regulates PRC2-mediated chromatin reprogramming during mouse development
    Article Snippet: .. The 3xFlag sequence was then ligated into the digested eGFP-Usp9x plasmid (Takara DNA ligation kit #6023). .. ES cell targeting Vectors were amplified by transformation into Stbl3 competent cells (Invitrogen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa t4 dna ligase
    Stimulation of DNA ligation by histone H1 and deletion mutants. The 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 1–15 nM ( left to right ) histone H1 (fl) or deletion mutants within the highly basic C-terminus, followed by ligation by <t>T4</t> DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer.
    T4 Dna Ligase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/TaKaRa
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Stimulation of DNA ligation by histone H1 and deletion mutants. The 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 1–15 nM ( left to right ) histone H1 (fl) or deletion mutants within the highly basic C-terminus, followed by ligation by T4 DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer.

    Journal: PLoS ONE

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein

    doi: 10.1371/journal.pone.0138774

    Figure Lengend Snippet: Stimulation of DNA ligation by histone H1 and deletion mutants. The 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 1–15 nM ( left to right ) histone H1 (fl) or deletion mutants within the highly basic C-terminus, followed by ligation by T4 DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer.

    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Techniques: DNA Ligation, Labeling, Incubation, Ligation, Electrophoresis

    Histone H1 inhibits the ability of HMGB1 to bend DNA. A , formation of DNA circles by HMGB1 is inhibited by the full-length histone H1 (DNA circularization assay). The 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 5 nM HMGB1, followed by titration with increasing concentrations of H1 (0.2–15 nM, left to right ) and ligation by T4 DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. Panels B - E , DNA circularization assays in the presence of the full-length histone H1(fl) or peptides H1Δ24, H1Δ48 and H1Δ72. The percentage of DNA circles by reduced or oxidized HMGB1 or HMGB1ΔC (50 nM) in the presence of increasing concentrations of H1 or H1 peptides (1–15 nM, left to right ) is indicated. The percentage of the minicircles formed by HMGB1 or HMGB1ΔC in the absence of H1 or peptides was arbitrary set to 100%. Oxidized HMGB1 or HMGB1ΔC proteins are indicated in red.

    Journal: PLoS ONE

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein

    doi: 10.1371/journal.pone.0138774

    Figure Lengend Snippet: Histone H1 inhibits the ability of HMGB1 to bend DNA. A , formation of DNA circles by HMGB1 is inhibited by the full-length histone H1 (DNA circularization assay). The 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 5 nM HMGB1, followed by titration with increasing concentrations of H1 (0.2–15 nM, left to right ) and ligation by T4 DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. Panels B - E , DNA circularization assays in the presence of the full-length histone H1(fl) or peptides H1Δ24, H1Δ48 and H1Δ72. The percentage of DNA circles by reduced or oxidized HMGB1 or HMGB1ΔC (50 nM) in the presence of increasing concentrations of H1 or H1 peptides (1–15 nM, left to right ) is indicated. The percentage of the minicircles formed by HMGB1 or HMGB1ΔC in the absence of H1 or peptides was arbitrary set to 100%. Oxidized HMGB1 or HMGB1ΔC proteins are indicated in red.

    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Techniques: Labeling, Incubation, Titration, Ligation, Electrophoresis

    The effect of oxidization and mutation of Cys22/Cys44 or Phe37 of HMGB1ΔC on DNA bending. A , the 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 2, 5, 10, 15, 25, 50 and 100 nM of HMGB1 lacking the acidic C-tail (HMGB1ΔC, left to right ), followed by ligation by T4 DNA ligase (DNA circularization assay). Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. B , percentage of DNA circles formed by reduced (black triangle) or oxidized (empty triangle) HMGB1ΔC, as compared to DNA circles formed under the same conditions by reduced (black circles) or oxidized (empty circles) full-length HMGB1. The percentage of the minicircles formed at 100 nM HMGB1 was arbitrary set to 100% (each of the curves represent an average of three independent experiments). C , representative circularization assay using reduced HMGB1ΔC, oxidized HMGB1ΔC, and HMGB1ΔC(F37A). Concentrations of proteins were 5, 10, 25, 50 and 100 nM ( left to right ).

    Journal: PLoS ONE

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein

    doi: 10.1371/journal.pone.0138774

    Figure Lengend Snippet: The effect of oxidization and mutation of Cys22/Cys44 or Phe37 of HMGB1ΔC on DNA bending. A , the 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 2, 5, 10, 15, 25, 50 and 100 nM of HMGB1 lacking the acidic C-tail (HMGB1ΔC, left to right ), followed by ligation by T4 DNA ligase (DNA circularization assay). Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. B , percentage of DNA circles formed by reduced (black triangle) or oxidized (empty triangle) HMGB1ΔC, as compared to DNA circles formed under the same conditions by reduced (black circles) or oxidized (empty circles) full-length HMGB1. The percentage of the minicircles formed at 100 nM HMGB1 was arbitrary set to 100% (each of the curves represent an average of three independent experiments). C , representative circularization assay using reduced HMGB1ΔC, oxidized HMGB1ΔC, and HMGB1ΔC(F37A). Concentrations of proteins were 5, 10, 25, 50 and 100 nM ( left to right ).

    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Techniques: Mutagenesis, Labeling, Incubation, Ligation, Electrophoresis

    The effect of oxidization and mutation of Cys22/Cys44 or Phe37 of HMGB1 on DNA bending. A , the 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was preincubated with 2, 5, 10, 15, 25, 50 and 100 nM HMGB1 proteins ( left to right ), followed by ligation by T4 DNA ligase (DNA circularization assay). Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. B , percentage of DNA circles formed by reduced HMGB1, oxidized HMGB1 or HMGB1(Cys22A/Cys44A) mutant. The percentage of the minicircles formed at 100 nM HMGB1 was arbitrary set to 100% (each of the curves represent an average of three independent experiments). C , representative circularization assay using reduced HMGB1 and HMGB1(F37A) mutant (5, 20, 50 and 100 nM HMGB1, left to right ). C22/C44, HMGB1(Cys22A/Cys44A) mutant.

    Journal: PLoS ONE

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein

    doi: 10.1371/journal.pone.0138774

    Figure Lengend Snippet: The effect of oxidization and mutation of Cys22/Cys44 or Phe37 of HMGB1 on DNA bending. A , the 5´-end 32 P-labeled 123-bp DNA fragment (~1 nM) was preincubated with 2, 5, 10, 15, 25, 50 and 100 nM HMGB1 proteins ( left to right ), followed by ligation by T4 DNA ligase (DNA circularization assay). Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer. B , percentage of DNA circles formed by reduced HMGB1, oxidized HMGB1 or HMGB1(Cys22A/Cys44A) mutant. The percentage of the minicircles formed at 100 nM HMGB1 was arbitrary set to 100% (each of the curves represent an average of three independent experiments). C , representative circularization assay using reduced HMGB1 and HMGB1(F37A) mutant (5, 20, 50 and 100 nM HMGB1, left to right ). C22/C44, HMGB1(Cys22A/Cys44A) mutant.

    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Techniques: Mutagenesis, Labeling, Ligation, Electrophoresis

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: In summary, we carried out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on ferromagnetic particles and found that the ligation efficiency was increased under a radio frequency alternating magnetic field caused by heat generation from the particles.

    Techniques: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: In summary, we carried out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on ferromagnetic particles and found that the ligation efficiency was increased under a radio frequency alternating magnetic field caused by heat generation from the particles.

    Techniques: DNA Ligation, Ligation

    Ligation assay by T4 DNA ligase. Time scan curves of liga tion catalyzed by various concentrations of T4 DNA ligase. The curves from top to bottom are those obtained with different T4 DNA ligase concentrations: 2.3, 0.23, 0.115, 6.9 × 10 –2 , 2.3 × 10 –2 , 1.2 × 10 –2 , 6.9 × 10 –3 , 2.3 × 10 –3 , 1.2 × 10 –3 and 2.3 × 10 –4 U/ml. (Insert) The initial ligation velocity is plotted as a function of the concentration of T4 DNA ligase.

    Journal: Nucleic Acids Research

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons

    doi: 10.1093/nar/gng146

    Figure Lengend Snippet: Ligation assay by T4 DNA ligase. Time scan curves of liga tion catalyzed by various concentrations of T4 DNA ligase. The curves from top to bottom are those obtained with different T4 DNA ligase concentrations: 2.3, 0.23, 0.115, 6.9 × 10 –2 , 2.3 × 10 –2 , 1.2 × 10 –2 , 6.9 × 10 –3 , 2.3 × 10 –3 , 1.2 × 10 –3 and 2.3 × 10 –4 U/ml. (Insert) The initial ligation velocity is plotted as a function of the concentration of T4 DNA ligase.

    Article Snippet: Two samples containing 10 µl reaction solutions were moved from each sample before adding T4 DNA ligase, and after the ligation process had taken place for 360 s with the addition of ligase.

    Techniques: Ligation, Concentration Assay

    Real-time fluorescence scans and corresponding gel electrophoresis. (Left) Curve A is a time scan of fluorescence intensity of MB with N1; B is of MB with N2 and N4; C is of MB with N2 and N3; curve D is of MB itself. t 0 is the time when T4 DNA ligase is added into the MB/oligo solution. (Right) Gel electrophoresis images. Lanes 1 and 2 are for sample D; 3 and 4 for sample C; 5 and 6 for sample B; and 7 and 8 for sample A. Lanes 1, 3, 5 and 7 represent samples D, C, B and A before the addition of T4 DNA ligase, while lanes 2, 4, 6 and 8 represent corresponding samples obtained at 360 s after the addition of ligase. There is a major difference between lanes 5 and 6, while there is basically no difference for all the other pairs.

    Journal: Nucleic Acids Research

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons

    doi: 10.1093/nar/gng146

    Figure Lengend Snippet: Real-time fluorescence scans and corresponding gel electrophoresis. (Left) Curve A is a time scan of fluorescence intensity of MB with N1; B is of MB with N2 and N4; C is of MB with N2 and N3; curve D is of MB itself. t 0 is the time when T4 DNA ligase is added into the MB/oligo solution. (Right) Gel electrophoresis images. Lanes 1 and 2 are for sample D; 3 and 4 for sample C; 5 and 6 for sample B; and 7 and 8 for sample A. Lanes 1, 3, 5 and 7 represent samples D, C, B and A before the addition of T4 DNA ligase, while lanes 2, 4, 6 and 8 represent corresponding samples obtained at 360 s after the addition of ligase. There is a major difference between lanes 5 and 6, while there is basically no difference for all the other pairs.

    Article Snippet: Two samples containing 10 µl reaction solutions were moved from each sample before adding T4 DNA ligase, and after the ligation process had taken place for 360 s with the addition of ligase.

    Techniques: Fluorescence, Nucleic Acid Electrophoresis