Structured Review

Stratagene t4 ligase
T4 Ligase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/Stratagene
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

Images

Related Articles

Selection:

Article Title: High-Fidelity Correction of Mutations at Multiple Chromosomal Positions by Adeno-Associated Virus Vectors
Article Snippet: .. Integrated retrovirus target loci were rescued by digestion of genomic DNA with Eco RI, circularization with T4 ligase, transfer to Escherichia coli XL1Blue MRF′ (Stratagene), and selection for kanamycin resistance as previously described ( ). ..

Agarose Gel Electrophoresis:

Article Title: PHR1 and PHR2 of Candida albicans Encode Putative Glycosidases Required for Proper Cross-Linking of ?-1,3- and ?-1,6-Glucans
Article Snippet: .. The desired product was purified by agarose gel electrophoresis, circularized by treatment with polynucleotide kinase and T4 ligase, and transformed into Escherichia coli XL1-Blue (Strategene). .. The presence of the desired base changes was verified by nucleotide sequence determination.

Ligation:

Article Title: Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiae
Article Snippet: .. Cassettes were concatenated, favoring the ligation of sticky ends, by adjusting the reaction to 5 mM ATP (Fermentas), adding T4 ligase (Stratagene) (25 mU/μg DNA starting material), and incubating for 3 h at RT, yielding high molecular weight DNA. .. To create fresh sticky ends for adding YAC arms, the DNA was then briefly submitted to a 3 min partial Asc I digest (6 mU/μg DNA starting material) before adding YAC arm DNA at a w/w ratio of 1:10, based on starting material of YAC vector and Entry vector, respectively, and performing a final ligation reaction, all in the same vial.

Purification:

Article Title: PHR1 and PHR2 of Candida albicans Encode Putative Glycosidases Required for Proper Cross-Linking of ?-1,3- and ?-1,6-Glucans
Article Snippet: .. The desired product was purified by agarose gel electrophoresis, circularized by treatment with polynucleotide kinase and T4 ligase, and transformed into Escherichia coli XL1-Blue (Strategene). .. The presence of the desired base changes was verified by nucleotide sequence determination.

Article Title: The Regulated Outer Membrane Protein Omp21 from Comamonas acidovorans Is Identified as a Member of a New Family of Eight-Stranded ?-Sheet Proteins by Its Sequence and Properties
Article Snippet: .. After incubation at 37°C overnight, the digested DNA was purified by means of the gel extraction kit from Bio-Rad and religated with 400 U of T4 ligase (Stratagene) in a 100-μl volume. .. These DNA fragments, together with complementary primers derived from the 125-bp fragment, were used for inverse PCR.

Marker:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Incubation:

Article Title: The Regulated Outer Membrane Protein Omp21 from Comamonas acidovorans Is Identified as a Member of a New Family of Eight-Stranded ?-Sheet Proteins by Its Sequence and Properties
Article Snippet: .. After incubation at 37°C overnight, the digested DNA was purified by means of the gel extraction kit from Bio-Rad and religated with 400 U of T4 ligase (Stratagene) in a 100-μl volume. .. These DNA fragments, together with complementary primers derived from the 125-bp fragment, were used for inverse PCR.

Molecular Weight:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Article Title: Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiae
Article Snippet: .. Cassettes were concatenated, favoring the ligation of sticky ends, by adjusting the reaction to 5 mM ATP (Fermentas), adding T4 ligase (Stratagene) (25 mU/μg DNA starting material), and incubating for 3 h at RT, yielding high molecular weight DNA. .. To create fresh sticky ends for adding YAC arms, the DNA was then briefly submitted to a 3 min partial Asc I digest (6 mU/μg DNA starting material) before adding YAC arm DNA at a w/w ratio of 1:10, based on starting material of YAC vector and Entry vector, respectively, and performing a final ligation reaction, all in the same vial.

Sequencing:

Article Title: FOXP3+ regulatory T cell development and function require histone/protein deacetylase 3
Article Snippet: .. The HDAC3 cDNA — cut from pCMV-Sport6 using NotI (R0189S, New England Biolabs) and SalI (R0138S, New England Biolabs) and with a blunt end added using DNA polymerase I Klenow (M0210S, New England Biolabs) in the presence of dNTPs — was ligated into the MinR-1 vector using T4 ligase (203003, Stratagene), and plasmid sequence verified. .. Retro­viruses were generated by cotransfection of MinR1-HDAC3 or parental MinR1 vector with pCLeco (Invitrogen) helper plasmid into the 293T Phoenix ecotropic packaging cell line (ATCC) using Lipofectamine 2000 (11668-019, Invitrogen).

Gel Extraction:

Article Title: The Regulated Outer Membrane Protein Omp21 from Comamonas acidovorans Is Identified as a Member of a New Family of Eight-Stranded ?-Sheet Proteins by Its Sequence and Properties
Article Snippet: .. After incubation at 37°C overnight, the digested DNA was purified by means of the gel extraction kit from Bio-Rad and religated with 400 U of T4 ligase (Stratagene) in a 100-μl volume. .. These DNA fragments, together with complementary primers derived from the 125-bp fragment, were used for inverse PCR.

Transformation Assay:

Article Title: PHR1 and PHR2 of Candida albicans Encode Putative Glycosidases Required for Proper Cross-Linking of ?-1,3- and ?-1,6-Glucans
Article Snippet: .. The desired product was purified by agarose gel electrophoresis, circularized by treatment with polynucleotide kinase and T4 ligase, and transformed into Escherichia coli XL1-Blue (Strategene). .. The presence of the desired base changes was verified by nucleotide sequence determination.

Plasmid Preparation:

Article Title: Characterization of the Enzymatic Component of the ADP-Ribosyltransferase Toxin CDTa from Clostridium difficile
Article Snippet: .. Oligonucleotides were purchased from MWG (Ebersberg, Germany), the pGEX2T vector (included in the glutathione S -transferase [GST] Gene Fusion System) and glutathione Sepharose 4B were obtained from Pharmacia Biotech (Uppsala, Sweden), the DNA molecular weight marker (lambda Hin dIII) and restriction enzymes were from Roche Diagnostics (Mannheim, Germany), and T4 ligase and competent E. coli cells were from Stratagene (Heidelberg, Germany). .. The Topo-TA-Cloning kit was from Invitrogen (Groningen, The Netherlands).

Article Title: FOXP3+ regulatory T cell development and function require histone/protein deacetylase 3
Article Snippet: .. The HDAC3 cDNA — cut from pCMV-Sport6 using NotI (R0189S, New England Biolabs) and SalI (R0138S, New England Biolabs) and with a blunt end added using DNA polymerase I Klenow (M0210S, New England Biolabs) in the presence of dNTPs — was ligated into the MinR-1 vector using T4 ligase (203003, Stratagene), and plasmid sequence verified. .. Retro­viruses were generated by cotransfection of MinR1-HDAC3 or parental MinR1 vector with pCLeco (Invitrogen) helper plasmid into the 293T Phoenix ecotropic packaging cell line (ATCC) using Lipofectamine 2000 (11668-019, Invitrogen).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Stratagene t4 dna ligase
    The URMAC method. This illustration depicts a hypothetical insertion within a Modification Target (black lines) in the Original DNA plasmid. PCR #1 generates the Starter DNA copy of the Modification Target including the flanking unique restriction sites, X and Y. It is produced by a thermostable DNA polymerase using the Starter Primers , SP1 and SP2 (black arrows). The Starter DNA is circularized with <t>T4</t> DNA ligase to generate the Closed Starter DNA . The Closed Circular DNA serves as the template for PCR #2, directed by the Opener Primers , OP1 and OP2, to produce the mutated Intermediate DNA . In this illustration, OP1 has incorporated an insertion mutation by having the sequence of interest (depicted as an open box) attached to its 5′ terminus. The Intermediate DNA is circularized with T4 DNA ligase. The SP1 and SP2 primers are used in the enrichment PCR step to amplify the Linear Modified DNA . The Linear Modified DNA , and the original plasmid are digested with the restriction enzymes that cleave at the unique restriction sites, X and Y, and the appropriate fragments are ligated to produce the Modified Original DNA .
    T4 Dna Ligase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Stratagene
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Stratagene t4 ligase
    The URMAC method. This illustration depicts a hypothetical insertion within a Modification Target (black lines) in the Original DNA plasmid. PCR #1 generates the Starter DNA copy of the Modification Target including the flanking unique restriction sites, X and Y. It is produced by a thermostable DNA polymerase using the Starter Primers , SP1 and SP2 (black arrows). The Starter DNA is circularized with <t>T4</t> DNA ligase to generate the Closed Starter DNA . The Closed Circular DNA serves as the template for PCR #2, directed by the Opener Primers , OP1 and OP2, to produce the mutated Intermediate DNA . In this illustration, OP1 has incorporated an insertion mutation by having the sequence of interest (depicted as an open box) attached to its 5′ terminus. The Intermediate DNA is circularized with T4 DNA ligase. The SP1 and SP2 primers are used in the enrichment PCR step to amplify the Linear Modified DNA . The Linear Modified DNA , and the original plasmid are digested with the restriction enzymes that cleave at the unique restriction sites, X and Y, and the appropriate fragments are ligated to produce the Modified Original DNA .
    T4 Ligase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Stratagene
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    The URMAC method. This illustration depicts a hypothetical insertion within a Modification Target (black lines) in the Original DNA plasmid. PCR #1 generates the Starter DNA copy of the Modification Target including the flanking unique restriction sites, X and Y. It is produced by a thermostable DNA polymerase using the Starter Primers , SP1 and SP2 (black arrows). The Starter DNA is circularized with T4 DNA ligase to generate the Closed Starter DNA . The Closed Circular DNA serves as the template for PCR #2, directed by the Opener Primers , OP1 and OP2, to produce the mutated Intermediate DNA . In this illustration, OP1 has incorporated an insertion mutation by having the sequence of interest (depicted as an open box) attached to its 5′ terminus. The Intermediate DNA is circularized with T4 DNA ligase. The SP1 and SP2 primers are used in the enrichment PCR step to amplify the Linear Modified DNA . The Linear Modified DNA , and the original plasmid are digested with the restriction enzymes that cleave at the unique restriction sites, X and Y, and the appropriate fragments are ligated to produce the Modified Original DNA .

    Journal: PLoS ONE

    Article Title: Efficient method for site-directed mutagenesis in large plasmids without subcloning

    doi: 10.1371/journal.pone.0177788

    Figure Lengend Snippet: The URMAC method. This illustration depicts a hypothetical insertion within a Modification Target (black lines) in the Original DNA plasmid. PCR #1 generates the Starter DNA copy of the Modification Target including the flanking unique restriction sites, X and Y. It is produced by a thermostable DNA polymerase using the Starter Primers , SP1 and SP2 (black arrows). The Starter DNA is circularized with T4 DNA ligase to generate the Closed Starter DNA . The Closed Circular DNA serves as the template for PCR #2, directed by the Opener Primers , OP1 and OP2, to produce the mutated Intermediate DNA . In this illustration, OP1 has incorporated an insertion mutation by having the sequence of interest (depicted as an open box) attached to its 5′ terminus. The Intermediate DNA is circularized with T4 DNA ligase. The SP1 and SP2 primers are used in the enrichment PCR step to amplify the Linear Modified DNA . The Linear Modified DNA , and the original plasmid are digested with the restriction enzymes that cleave at the unique restriction sites, X and Y, and the appropriate fragments are ligated to produce the Modified Original DNA .

    Article Snippet: Following ligation with T4 DNA ligase, Opener Primer pairs ( ) and Pfu Turbo polymerase (Stratagene) were used to generate the three Intermediate DNAs under these conditions: denaturation at 95° for 2 min followed by 25 cycles of 95°C, 20 sec; 51°C, 30 sec; and 72°C, 3 min, followed by 72°C, 10 min, and finally 4°C.

    Techniques: Modification, Plasmid Preparation, Polymerase Chain Reaction, Produced, Mutagenesis, Sequencing