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GE Healthcare t4 ligase
T4 Ligase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/GE Healthcare
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

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Ligation:

Article Title: Genomic Diversity of Clinical and Environmental Vibrio cholerae Strains Isolated in Brazil between 1991 and 2001 as Revealed by Fluorescent Amplified Fragment Length Polymorphism Analysis
Article Snippet: .. Briefly, 1 μg of high-molecular-mass DNA was digested with Taq I and Apa I (Amersham Pharmacia Biotech, Uppsala, Sweden) followed by ligation of restriction half-site-specific adapters to all restriction fragments with T4 ligase (Amersham Pharmacia Biotech). .. An aliquot of 2.5 μl of template was mixed with 0.8 μl of A01-6FAM primer (5′-GACTGCGTACAGGCCC A -3′; 1 μM), 0.8 μl of T01-ABI (5′-CGATGAGTCCTGACCGA A -3′; 5 μM), and 12 μl of amplification core mix (Applied Biosystems).

Article Title: Strain-specific telomere length revealed by single telomere length analysis in Caenorhabditis elegans
Article Snippet: .. Ligation was carried out as follows: a mix of 20 ng of DNA (quantitated by PicoGreen® dsDNA Quantitation Kit, Molecular Probes) and 1 μl of 10 μM telorette (Table ) in a 2 μl volume was incubated at 60°C for 10 min. Ligation was carried out at 35°C for 12–15 h with 0.2 μl of 1 U/μl T4 ligase (Amersham) and 0.4 μl of 10× manufacturer's ligation buffer in a final volume of 4 μl. .. Five adult worms or a single adult worm were lysed in 5 μl lysis buffer B (1× PCR buffer (500 mM Tris pH 8.3, 350 μM Na2 HPO4 , 0.5% Tween-20), 1.5 mM MgCl2 , 0.06 mg/ml Proteinase K) at 57°C for 60 min.

Quantitation Assay:

Article Title: Strain-specific telomere length revealed by single telomere length analysis in Caenorhabditis elegans
Article Snippet: .. Ligation was carried out as follows: a mix of 20 ng of DNA (quantitated by PicoGreen® dsDNA Quantitation Kit, Molecular Probes) and 1 μl of 10 μM telorette (Table ) in a 2 μl volume was incubated at 60°C for 10 min. Ligation was carried out at 35°C for 12–15 h with 0.2 μl of 1 U/μl T4 ligase (Amersham) and 0.4 μl of 10× manufacturer's ligation buffer in a final volume of 4 μl. .. Five adult worms or a single adult worm were lysed in 5 μl lysis buffer B (1× PCR buffer (500 mM Tris pH 8.3, 350 μM Na2 HPO4 , 0.5% Tween-20), 1.5 mM MgCl2 , 0.06 mg/ml Proteinase K) at 57°C for 60 min.

Incubation:

Article Title: Strain-specific telomere length revealed by single telomere length analysis in Caenorhabditis elegans
Article Snippet: .. Ligation was carried out as follows: a mix of 20 ng of DNA (quantitated by PicoGreen® dsDNA Quantitation Kit, Molecular Probes) and 1 μl of 10 μM telorette (Table ) in a 2 μl volume was incubated at 60°C for 10 min. Ligation was carried out at 35°C for 12–15 h with 0.2 μl of 1 U/μl T4 ligase (Amersham) and 0.4 μl of 10× manufacturer's ligation buffer in a final volume of 4 μl. .. Five adult worms or a single adult worm were lysed in 5 μl lysis buffer B (1× PCR buffer (500 mM Tris pH 8.3, 350 μM Na2 HPO4 , 0.5% Tween-20), 1.5 mM MgCl2 , 0.06 mg/ml Proteinase K) at 57°C for 60 min.

Purification:

Article Title: Nanofabricated Racks of Aligned and Anchored DNA Substrates for Single-Molecule Imaging
Article Snippet: .. Reaction mixtures that included T4 ligase were then heat-inactivated at 65 °C for 10 min, and the ligated DNA products were purified over a Sephacryl S200HR column (GE Healthcare) run in 10 mM Tris-HCl (pH 7.8), 1 mM EDTA, and 150 mM NaCl. ..

Article Title: Recombinant gas vesicles from Halobacterium sp. displaying SIV peptides demonstrate biotechnology potential as a pathogen peptide delivery vehicle
Article Snippet: .. Fragments were gel purified and separately ligated with T4 ligase (Amersham Pharmacia Biotech, Inc; Piscataway, NJ). .. E. coli cells (DH5α) were rendered competent using the standard treatment with calcium chloride as detailed previously [ , ], then transformed and the incorporated SIV DNA amplified.

Article Title: Genotypic Characterization of Bradyrhizobium Strains Nodulating Endemic Woody Legumes of the Canary Islands by PCR-Restriction Fragment Length Polymorphism Analysis of Genes Encoding 16S rRNA (16S rDNA) and 16S-23S rDNA Intergenic Spacers, Repetitive Extragenic Palindromic PCR Genomic Fingerprinting, and Partial 16S rDNA Sequencing
Article Snippet: .. The restriction fragments were purified from low-gelling-point agarose (Biozym) gels with Promega’s Wizard PCR-Prep columns (Promega) and ligated into pBluescript KS(+) (Stratagene, La Jolla, Calif.) with T4 ligase (USB-Amersham). .. The ligation mixture was used to transform CaCl2 -competent E. coli DH5α cells ( ).

Generated:

Article Title: The Cyclic AMP-Cyclic AMP Receptor Protein Complex Regulates Activity of the traJ Promoter of the Escherichia coli Conjugative Plasmid pRK100
Article Snippet: .. The DNA fragment generated was digested with Mse I, and the restriction fragments were ligated with T4 ligase (Amersham Pharmacia Biotech). .. PCR amplification of the ligation mixture with primers lacZ-1 and lacZ-2 yielded several products, including the desired 158-bp Δ lacZ product.

other:

Article Title: Reactivity of Toluate Dioxygenase with Substituted Benzoates and Dioxygen
Article Snippet: Restriction enzymes, Pfu I polymerase, and T4 ligase were from Amersham Pharmacia Biotech and Stratagene.

Polymerase Chain Reaction:

Article Title: Genotypic Characterization of Bradyrhizobium Strains Nodulating Endemic Woody Legumes of the Canary Islands by PCR-Restriction Fragment Length Polymorphism Analysis of Genes Encoding 16S rRNA (16S rDNA) and 16S-23S rDNA Intergenic Spacers, Repetitive Extragenic Palindromic PCR Genomic Fingerprinting, and Partial 16S rDNA Sequencing
Article Snippet: .. The restriction fragments were purified from low-gelling-point agarose (Biozym) gels with Promega’s Wizard PCR-Prep columns (Promega) and ligated into pBluescript KS(+) (Stratagene, La Jolla, Calif.) with T4 ligase (USB-Amersham). .. The ligation mixture was used to transform CaCl2 -competent E. coli DH5α cells ( ).

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  • 92
    GE Healthcare t4 rna ligase
    RNA-DNA ligation with <t>T4</t> RNA ligase. ( A ) The 21- to 24-nt smRNAs from infected HC-Pro + or HC-Pro - plants (lanes 5-12) or the 24-nt smRNAs from both infected and uninfected HC-Pro + and HC-Pro - plants (lanes 13-28) were gel-purified and ligated to a 16-nt
    T4 Rna Ligase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase/product/GE Healthcare
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    88
    GE Healthcare dna ligases
    Effect of ligase inhibitors on replication and repair reactions catalyzed by human cell extracts A The flap substrate (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 2, Mock) or presence of 100 μM of; lane 3, L189 ; lane 4, L67 ; lane 5, L82 . hLigI (lane 6, I-dp) and hLigIIIα (lane 7, III-dp) were immunodepeleted from the cell extracts prior to incubation with the <t>DNA</t> substrate. Lane 1, DNA substrate alone (Sub). The positions of the DNA substrate (24 mer), cleaved product (18 mer) and fully repaired product (43 mer) are shown. B . The linear DNA substrate with an incised AP site (0.3 pmol) was incubated with a cell extract (20 μg) and [α 32 P]dTTP in the absence (lane l, Mock) or presence of 100 μM of; lane 2, L189 ; lane 3, L67 ; lane 4, L82 . hLigI (lane 5, I-dp) and hLigIIIα (lane 6, III-dp) were <t>immunodepleted</t> from the cell extracts prior to incubation with the DNA substrate. The positions of the single nucleotide insertion reaction intermediate (31 mer, Incorporated) and the ligated product (73 mer, Repaired) are indicated. C. A 1 kb fragment with cohesive ends (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 3, Mock) or presence of 100 μM of; lane 4, L189 ; lane 5, L67 ; lane 6, L82 . hLigI (lane 7, I-dp) hLigIIIα (lane 8, III-dp) and hLigIV (lane 9, IV-dp) were immunodepleted from the cell extracts prior to incubation with the DNA substrate. Lane 1, molecular mass standard (M). Lane 2, DNA substrate alone (Sub). The positions of the DNA substrate and dimers and multimers of the substrate are indicated.
    Dna Ligases, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligases/product/GE Healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna ligases - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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    RNA-DNA ligation with T4 RNA ligase. ( A ) The 21- to 24-nt smRNAs from infected HC-Pro + or HC-Pro - plants (lanes 5-12) or the 24-nt smRNAs from both infected and uninfected HC-Pro + and HC-Pro - plants (lanes 13-28) were gel-purified and ligated to a 16-nt

    Journal:

    Article Title: Extensive 3? modification of plant small RNAs is modulated by helper component-proteinase expression

    doi: 10.1073/pnas.0506597102

    Figure Lengend Snippet: RNA-DNA ligation with T4 RNA ligase. ( A ) The 21- to 24-nt smRNAs from infected HC-Pro + or HC-Pro - plants (lanes 5-12) or the 24-nt smRNAs from both infected and uninfected HC-Pro + and HC-Pro - plants (lanes 13-28) were gel-purified and ligated to a 16-nt

    Article Snippet: Radiolabeled 21- to 24-nt smRNAs from virus-infected HC-Pro+ or HC-Pro- plants, together with the four gel-purified samples of 24-nt smRNAs (the same as used in ), were tested for their ability to serve as substrates for T4 RNA ligase.

    Techniques: DNA Ligation, Infection, Purification

    Effect of ligase inhibitors on replication and repair reactions catalyzed by human cell extracts A The flap substrate (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 2, Mock) or presence of 100 μM of; lane 3, L189 ; lane 4, L67 ; lane 5, L82 . hLigI (lane 6, I-dp) and hLigIIIα (lane 7, III-dp) were immunodepeleted from the cell extracts prior to incubation with the DNA substrate. Lane 1, DNA substrate alone (Sub). The positions of the DNA substrate (24 mer), cleaved product (18 mer) and fully repaired product (43 mer) are shown. B . The linear DNA substrate with an incised AP site (0.3 pmol) was incubated with a cell extract (20 μg) and [α 32 P]dTTP in the absence (lane l, Mock) or presence of 100 μM of; lane 2, L189 ; lane 3, L67 ; lane 4, L82 . hLigI (lane 5, I-dp) and hLigIIIα (lane 6, III-dp) were immunodepleted from the cell extracts prior to incubation with the DNA substrate. The positions of the single nucleotide insertion reaction intermediate (31 mer, Incorporated) and the ligated product (73 mer, Repaired) are indicated. C. A 1 kb fragment with cohesive ends (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 3, Mock) or presence of 100 μM of; lane 4, L189 ; lane 5, L67 ; lane 6, L82 . hLigI (lane 7, I-dp) hLigIIIα (lane 8, III-dp) and hLigIV (lane 9, IV-dp) were immunodepleted from the cell extracts prior to incubation with the DNA substrate. Lane 1, molecular mass standard (M). Lane 2, DNA substrate alone (Sub). The positions of the DNA substrate and dimers and multimers of the substrate are indicated.

    Journal: Cancer research

    Article Title: Rational Design of Human DNA Ligase Inhibitors that Target Cellular DNA Replication and Repair

    doi: 10.1158/0008-5472.CAN-07-6636

    Figure Lengend Snippet: Effect of ligase inhibitors on replication and repair reactions catalyzed by human cell extracts A The flap substrate (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 2, Mock) or presence of 100 μM of; lane 3, L189 ; lane 4, L67 ; lane 5, L82 . hLigI (lane 6, I-dp) and hLigIIIα (lane 7, III-dp) were immunodepeleted from the cell extracts prior to incubation with the DNA substrate. Lane 1, DNA substrate alone (Sub). The positions of the DNA substrate (24 mer), cleaved product (18 mer) and fully repaired product (43 mer) are shown. B . The linear DNA substrate with an incised AP site (0.3 pmol) was incubated with a cell extract (20 μg) and [α 32 P]dTTP in the absence (lane l, Mock) or presence of 100 μM of; lane 2, L189 ; lane 3, L67 ; lane 4, L82 . hLigI (lane 5, I-dp) and hLigIIIα (lane 6, III-dp) were immunodepleted from the cell extracts prior to incubation with the DNA substrate. The positions of the single nucleotide insertion reaction intermediate (31 mer, Incorporated) and the ligated product (73 mer, Repaired) are indicated. C. A 1 kb fragment with cohesive ends (0.1 pmol) was incubated with cell extract (20 μg) in the absence (lane 3, Mock) or presence of 100 μM of; lane 4, L189 ; lane 5, L67 ; lane 6, L82 . hLigI (lane 7, I-dp) hLigIIIα (lane 8, III-dp) and hLigIV (lane 9, IV-dp) were immunodepleted from the cell extracts prior to incubation with the DNA substrate. Lane 1, molecular mass standard (M). Lane 2, DNA substrate alone (Sub). The positions of the DNA substrate and dimers and multimers of the substrate are indicated.

    Article Snippet: Where indicated, DNA ligases were immunodepleted from the extracts as described ( ) using protein A or G Sepharose beads (GE healthcare) and anti-Lig1, anti-LigIII (GeneTex), or anti-LigIV (ABCAM) antibodies.

    Techniques: Incubation