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Boehringer Mannheim t4 ligase
T4 Ligase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase/product/Boehringer Mannheim
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
t4 ligase - by Bioz Stars, 2020-05
92/100 stars

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Ligation:

Article Title: Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis †
Article Snippet: .. Each ligation reaction was incubated overnight at 16°C in a 20-μl volume containing 1 U of T4 ligase (Boehringer Mannheim), and approximately 0.25 μg of PCR product and 0.25 μg of pComb3 vector. .. The ligated DNA molecules (2 μl each) were then transformed into E. coli XL-1 Blue MRF′ using an Electroporator II (Invitrogen, Carlsbad, Calif.).

Article Title: Dynamic Regulation of cpg15 during Activity-Dependent Synaptic Development in the Mammalian Visual System
Article Snippet: .. Several fractions were selected, and T4 ligase (Boehringer Mannheim) was used for ligation into Xba I-digested and calf alkaline phosphatase (Stratagene, La Jolla, CA)–treated ZAP express vector (Stratagene). .. Ligated DNA was packaged using Gigapack III gold packaging extract (Stratagene); 600,000 independent plaque-forming units were obtained and amplified.

Construct:

Article Title: Analysis of human peripheral blood T cells and single-cell-derived T cell clones uncovers extensive clonal CpG island methylation heterogeneity throughout the genome
Article Snippet: .. The genomic DNA used to construct the library was extracted from peripheral blood lymphocytes from the primary subject, was first digested with Not I, and was ligated to the Not I- and Eco RV-digested plasmid vector pBluescript SK(+) (Strategene) with T4 ligase (Boehringer Mannheim). .. After the first ligation and phenol/chloroform extraction, the DNA was digested with Eco RV and was purified with phenol/chloroform extraction-ethanol precipitation.

Incubation:

Article Title: Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis †
Article Snippet: .. Each ligation reaction was incubated overnight at 16°C in a 20-μl volume containing 1 U of T4 ligase (Boehringer Mannheim), and approximately 0.25 μg of PCR product and 0.25 μg of pComb3 vector. .. The ligated DNA molecules (2 μl each) were then transformed into E. coli XL-1 Blue MRF′ using an Electroporator II (Invitrogen, Carlsbad, Calif.).

other:

Article Title: Understanding the Molecular Epidemiology of the Footrot Pathogen Dichelobacter nodosus To Support Control and Eradication Programs ▿ To Support Control and Eradication Programs ▿ †
Article Snippet: T4 ligase was denatured at 65°C for 20 min.

Polymerase Chain Reaction:

Article Title: Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis †
Article Snippet: .. Each ligation reaction was incubated overnight at 16°C in a 20-μl volume containing 1 U of T4 ligase (Boehringer Mannheim), and approximately 0.25 μg of PCR product and 0.25 μg of pComb3 vector. .. The ligated DNA molecules (2 μl each) were then transformed into E. coli XL-1 Blue MRF′ using an Electroporator II (Invitrogen, Carlsbad, Calif.).

Plasmid Preparation:

Article Title: Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis †
Article Snippet: .. Each ligation reaction was incubated overnight at 16°C in a 20-μl volume containing 1 U of T4 ligase (Boehringer Mannheim), and approximately 0.25 μg of PCR product and 0.25 μg of pComb3 vector. .. The ligated DNA molecules (2 μl each) were then transformed into E. coli XL-1 Blue MRF′ using an Electroporator II (Invitrogen, Carlsbad, Calif.).

Article Title: Dynamic Regulation of cpg15 during Activity-Dependent Synaptic Development in the Mammalian Visual System
Article Snippet: .. Several fractions were selected, and T4 ligase (Boehringer Mannheim) was used for ligation into Xba I-digested and calf alkaline phosphatase (Stratagene, La Jolla, CA)–treated ZAP express vector (Stratagene). .. Ligated DNA was packaged using Gigapack III gold packaging extract (Stratagene); 600,000 independent plaque-forming units were obtained and amplified.

Article Title: Transfection of IL-2 and/or IL-12 genes into spleen in treatment of rat liver cancer
Article Snippet: .. The mIL-12 and hIL-2 gene fragments were connected respectively with pGEMTM -Teasy vector (Promega, USA) by T4 ligase (Boehringer Mannheim, Germany). .. The vector was transfected into E.coli TG1 competence cell and the positive clone was screened by PCR.

Article Title: Analysis of human peripheral blood T cells and single-cell-derived T cell clones uncovers extensive clonal CpG island methylation heterogeneity throughout the genome
Article Snippet: .. The genomic DNA used to construct the library was extracted from peripheral blood lymphocytes from the primary subject, was first digested with Not I, and was ligated to the Not I- and Eco RV-digested plasmid vector pBluescript SK(+) (Strategene) with T4 ligase (Boehringer Mannheim). .. After the first ligation and phenol/chloroform extraction, the DNA was digested with Eco RV and was purified with phenol/chloroform extraction-ethanol precipitation.

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    Boehringer Mannheim t4 dna ligase
    T4 DNA-ligase activity in the presence of SA. T4 DNA-ligase was treated or not with increasing SA concentrations (15 min at 25°C) and then incubated at 37°C for 1 min with the oligo substrate. ( A ) The oligo(dT) 16  multimers were separated in polyacrylamide/urea gels: T4 DNA-ligase without SA (lane 1) or incubated with increasing SA concentrations of 2.5(2), 5(3), 10(4), and 20(5) μM. ( B ) The activity was quantitated using an InstantImager (Packard). ( C ) Inhibition of enzyme-adenylate formation by SA. T4 DNA-ligase was incubated or not with increasing SA concentrations (15 min at 25°C) before the addition of [α- 32 P]ATP. The enzyme adenylate complexes were separated by electrophoresis and detected by autoradiography.
    T4 Dna Ligase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Boehringer Mannheim
    Average 92 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    T4 DNA-ligase activity in the presence of SA. T4 DNA-ligase was treated or not with increasing SA concentrations (15 min at 25°C) and then incubated at 37°C for 1 min with the oligo substrate. ( A ) The oligo(dT) 16  multimers were separated in polyacrylamide/urea gels: T4 DNA-ligase without SA (lane 1) or incubated with increasing SA concentrations of 2.5(2), 5(3), 10(4), and 20(5) μM. ( B ) The activity was quantitated using an InstantImager (Packard). ( C ) Inhibition of enzyme-adenylate formation by SA. T4 DNA-ligase was incubated or not with increasing SA concentrations (15 min at 25°C) before the addition of [α- 32 P]ATP. The enzyme adenylate complexes were separated by electrophoresis and detected by autoradiography.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Deficient DNA-ligase activity in the metabolic disease tyrosinemia type I

    doi:

    Figure Lengend Snippet: T4 DNA-ligase activity in the presence of SA. T4 DNA-ligase was treated or not with increasing SA concentrations (15 min at 25°C) and then incubated at 37°C for 1 min with the oligo substrate. ( A ) The oligo(dT) 16 multimers were separated in polyacrylamide/urea gels: T4 DNA-ligase without SA (lane 1) or incubated with increasing SA concentrations of 2.5(2), 5(3), 10(4), and 20(5) μM. ( B ) The activity was quantitated using an InstantImager (Packard). ( C ) Inhibition of enzyme-adenylate formation by SA. T4 DNA-ligase was incubated or not with increasing SA concentrations (15 min at 25°C) before the addition of [α- 32 P]ATP. The enzyme adenylate complexes were separated by electrophoresis and detected by autoradiography.

    Article Snippet: T4 DNA-ligase (2 units, 0.150 μg of proteins) was incubated (final volume, 10 μl) in a buffer containing 66 mM Hepes at pH 8.0, 10 mM MgCl2 , and increasing SA concentrations for 15 min at 25°C.

    Techniques: Activity Assay, Incubation, Inhibition, Electrophoresis, Autoradiography