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Promega t4 ligase enzyme
T4 Ligase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase enzyme/product/Promega
Average 91 stars, based on 3 article reviews
Price from $9.99 to $1999.99
t4 ligase enzyme - by Bioz Stars, 2020-05
91/100 stars

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Sequencing:

Article Title: Substrate- and pH-Specific Antifolate Transport Mediated by Organic Anion-Transporting Polypeptide 2B1 (OATP2B1-SLCO2B1)
Article Snippet: .. The isolated insert was ligated into pXoon using the T4 ligase enzyme (Promega) and verified by sequencing. .. The pXoon/OATP2B1 construct was linearized with XbaI enzyme (New England BioLabs) for the synthesis of capped sense OATP2B1 cRNA using the mMESSAGE mMachine system (Ambion, Austin, TX).

Isolation:

Article Title: Substrate- and pH-Specific Antifolate Transport Mediated by Organic Anion-Transporting Polypeptide 2B1 (OATP2B1-SLCO2B1)
Article Snippet: .. The isolated insert was ligated into the pcDNA3.1(+)hygro using T4 ligase enzyme (Promega, Madison, WI). .. The insert was verified by sequencing in the Albert Einstein Cancer Center Genomics Shared Resource.

Article Title: Substrate- and pH-Specific Antifolate Transport Mediated by Organic Anion-Transporting Polypeptide 2B1 (OATP2B1-SLCO2B1)
Article Snippet: .. The isolated insert was ligated into pXoon using the T4 ligase enzyme (Promega) and verified by sequencing. .. The pXoon/OATP2B1 construct was linearized with XbaI enzyme (New England BioLabs) for the synthesis of capped sense OATP2B1 cRNA using the mMESSAGE mMachine system (Ambion, Austin, TX).

Binding Assay:

Article Title: Systems Approach Reveals Nuclear Factor Erythroid 2-Related Factor 2/Protein Kinase R Crosstalk in Human Cutaneous Leishmaniasis
Article Snippet: .. The pGL2-basic plasmid was also digested with the Bgl-II enzyme for the subsequent binding of the amplicons with T4 ligase enzyme (Promega). .. The cloned fragments and final vectors were then transformed into DH5α bacteria, and colonies were selected for further confirmation by sequencing.

Plasmid Preparation:

Article Title: Systems Approach Reveals Nuclear Factor Erythroid 2-Related Factor 2/Protein Kinase R Crosstalk in Human Cutaneous Leishmaniasis
Article Snippet: .. The pGL2-basic plasmid was also digested with the Bgl-II enzyme for the subsequent binding of the amplicons with T4 ligase enzyme (Promega). .. The cloned fragments and final vectors were then transformed into DH5α bacteria, and colonies were selected for further confirmation by sequencing.

Ligation:

Article Title: Microfluidics-based chromosome conformation capture (3C) technology for examining chromatin organization with a low quantity of cells
Article Snippet: .. After digestion, 250 μl ligation reaction solution was created by mixing 25 μl digested nuclei (∼4×105 ), 25 μl of 10× ligase buffer, 12.5 μl of 20% Triton X-100, 187 μl H2 O and 0.5 μl of T4 ligase enzyme (10U). .. 5μl of the solution was used to fill up the reaction chamber (0.6 μ l in volume).∼1000 nuclei were remained in the reaction chamber for on chip ligation.

Article Title: Microfluidics-based chromosome conformation capture (3C) technology for examining chromatin organization with a low quantity of cells
Article Snippet: .. For ligation reaction, the digestion solution in the loading chambers was replaced by ligation solution made by mixing 18 μl H2 O, 2 μl 10 × ligation enzyme buffer (1× ligation buffer: 30 mM Tris-HCl, 10 mM MgCl2 , 10 mM DTT, 1 mM ATP, pH=7.8) and 4 U T4 ligase enzyme (Promega, Cat. No. M1794) with all valves closed. .. Ligation reagents were diffused into the reaction chamber after opening the valves v3 and 4.

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  • 91
    Promega antibiotics t4 dna ligase
    Construction of recombinant plasmid pGPA. After cloning using T vector, an intact plasmid including the promoter Pglv was inserted into plasmid pGJ103 (3.2 kb), resulting in the construction of pGP (3.45 kb). The plasmid containing the genes of abaecin and TEV protein, with gmp and gsp, was digested with Eco RI and Bam HI, while pGP was digested with Eco RI and Bam HI. The two digested products were linked by <t>T4</t> DNA ligase, yielding recombinant plasmid pGPA.
    Antibiotics T4 Dna Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibiotics t4 dna ligase/product/Promega
    Average 91 stars, based on 579 article reviews
    Price from $9.99 to $1999.99
    antibiotics t4 dna ligase - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    Promega t4 ligase enzyme
    Construction of recombinant plasmid pGPA. After cloning using T vector, an intact plasmid including the promoter Pglv was inserted into plasmid pGJ103 (3.2 kb), resulting in the construction of pGP (3.45 kb). The plasmid containing the genes of abaecin and TEV protein, with gmp and gsp, was digested with Eco RI and Bam HI, while pGP was digested with Eco RI and Bam HI. The two digested products were linked by <t>T4</t> DNA ligase, yielding recombinant plasmid pGPA.
    T4 Ligase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase enzyme/product/Promega
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t4 ligase enzyme - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

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    Construction of recombinant plasmid pGPA. After cloning using T vector, an intact plasmid including the promoter Pglv was inserted into plasmid pGJ103 (3.2 kb), resulting in the construction of pGP (3.45 kb). The plasmid containing the genes of abaecin and TEV protein, with gmp and gsp, was digested with Eco RI and Bam HI, while pGP was digested with Eco RI and Bam HI. The two digested products were linked by T4 DNA ligase, yielding recombinant plasmid pGPA.

    Journal: Brazilian Journal of Microbiology

    Article Title: A novel expression vector for the secretion of abaecin in Bacillus subtilis

    doi: 10.1016/j.bjm.2017.01.009

    Figure Lengend Snippet: Construction of recombinant plasmid pGPA. After cloning using T vector, an intact plasmid including the promoter Pglv was inserted into plasmid pGJ103 (3.2 kb), resulting in the construction of pGP (3.45 kb). The plasmid containing the genes of abaecin and TEV protein, with gmp and gsp, was digested with Eco RI and Bam HI, while pGP was digested with Eco RI and Bam HI. The two digested products were linked by T4 DNA ligase, yielding recombinant plasmid pGPA.

    Article Snippet: Enzymes, chemicals, and antibiotics T4 DNA ligase, Taq DNA polymerase, and all restriction enzymes were purchased from Promega (WI, USA).

    Techniques: Recombinant, Plasmid Preparation, Clone Assay