Structured Review

TaKaRa t4 ligase buffer
T4 Ligase Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t4 ligase buffer/product/TaKaRa
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t4 ligase buffer - by Bioz Stars, 2020-02
99/100 stars

Images

Related Articles

Clone Assay:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C. .. The plasmids of surviving clones were isolated and the direction of the RfB fragment was verified using restriction digests to obtain the binary destination vector pCUbi1390RfB.

Amplification:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: The cycling parameters were: 94°C for 3 min, followed by 10 cycles of 94°C for 30 s, 64°C for 30 s with a 0.5°C decrement of the annealing temperature per cycle and 72°C for 30 s, followed by 30 cycles of 94°C for 30 s, 59°C for 30 s and 72°C for 30 s, and a final extension step at 72°C for 5 min. After amplification, PCR products were incubated with 2 U shrimp alkaline phosophatase (Fermentas, Vilnius, Lithuania) and 4 U exonuclease I (Fermentas, Vilnius, Lithuania) at 37°C for 1 h, and then denatured at 95°C for 10 min. .. A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China).

Synthesized:

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: In Vitro Selection against tRNA with a GADV Peptide Library against tRNA The DNA library was transcribed into mRNA using the T7 RiboMAX Express large scale RNA production system (Promega, Madison, WI, USA), and the synthesized mRNA was purified with an RNA purification kit (FavorPrep After Tri-18 reagent RNA clean-up kit, Favorgen, Ping-Tung, Taiwan). .. Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight.

Construct:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: Gateway expression vector construction The binary vector pCUbi1390 was digested with Spe I then blunted with T4 DNA polymerase and ligated with the Gateway Vector Conversion System Reading Frame Cassette B (RfB) fragment (Invitrogen, Carlsbad, CA) to construct a binary destination vector. .. The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C.

Incubation:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: The cycling parameters were: 94°C for 3 min, followed by 10 cycles of 94°C for 30 s, 64°C for 30 s with a 0.5°C decrement of the annealing temperature per cycle and 72°C for 30 s, followed by 30 cycles of 94°C for 30 s, 59°C for 30 s and 72°C for 30 s, and a final extension step at 72°C for 5 min. After amplification, PCR products were incubated with 2 U shrimp alkaline phosophatase (Fermentas, Vilnius, Lithuania) and 4 U exonuclease I (Fermentas, Vilnius, Lithuania) at 37°C for 1 h, and then denatured at 95°C for 10 min. .. A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China).

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: .. Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight. .. Translationof the linker-conjugated mRNAs was performed by an in vitro translation system with a Retic Lysate IVT kit (Retic Lysate IVT kit, Ambion, Austin, TX, USA) at 30 °C for 30 min. We did not modify this kit in order to synthesize our peptides composed of four species of amino acid.

Article Title: Rapid EST isolation from chromosome 1R of rye
Article Snippet: Hybridization of chromosome 1R DNA and cDNA of rye The DNA of 1R chromosome (1 μg) and cDNA of rye (1 μg) were digested by 10 U Sau 3AI (TaKaRa, Dalian) in 25 μl volume at 37°C overnight, respectively, followed by Sau 3AI inactivation at 70°C for 15 min. Digested 1R DNA and cDNA of rye were linked with HSA1 and HSA2 in 20 μl volume, respectively, by mixing 2.5 μl of Sau 3AI digested 1R DNA or cDNA of rye, 2 μl (10 μM) of adaptor HSA1or HSA2, 2 μl 10°C T4 ligase buffer (TaKaRa, Dalian), and 1 μl (3.5 U/μl) T4 ligase (TaKaRa, Dalian) and kept at 16°C overnight. .. The samples with HSA1 and HSA2 were precipitated and resuspended in 2 μl hybridization buffer (50 mM HEPES, pH 8.3; 0.5 M NaCl; 0.02 mM EDTA, pH 8.0; 10% PEG 8000, W/V), then denatured at 98°C for 10 min and incubated at 68°C for 10 h. Subsequently, two samples were mixed together as soon as possible and hybridized at 68°C for 10 h. Hybridized DNA-cDNA duplexes of 1R DNA and cDNA of rye were filled in the ends by using Taq DNA polymerase (TaKaRa, Dalian, 1 U/μl of hybridized DNA) at 72°C for 20 min.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL. .. Ligation reactions were incubated at 16 °C for 16 h. For subsequent PCR, 1 μL of digested and re-ligated DNA template was used in a total reaction volume of 25 μL with Tks Gflex DNA Polymerase (TaKaRa, Shiga, Japan).

Activity Assay:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Expressing:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: Paragraph title: Gateway expression vector construction ... The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C.

Transformation Assay:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C. .. The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C.

Hybridization:

Article Title: Rapid EST isolation from chromosome 1R of rye
Article Snippet: .. Hybridization of chromosome 1R DNA and cDNA of rye The DNA of 1R chromosome (1 μg) and cDNA of rye (1 μg) were digested by 10 U Sau 3AI (TaKaRa, Dalian) in 25 μl volume at 37°C overnight, respectively, followed by Sau 3AI inactivation at 70°C for 15 min. Digested 1R DNA and cDNA of rye were linked with HSA1 and HSA2 in 20 μl volume, respectively, by mixing 2.5 μl of Sau 3AI digested 1R DNA or cDNA of rye, 2 μl (10 μM) of adaptor HSA1or HSA2, 2 μl 10°C T4 ligase buffer (TaKaRa, Dalian), and 1 μl (3.5 U/μl) T4 ligase (TaKaRa, Dalian) and kept at 16°C overnight. .. The samples with HSA1 and HSA2 were precipitated and resuspended in 2 μl hybridization buffer (50 mM HEPES, pH 8.3; 0.5 M NaCl; 0.02 mM EDTA, pH 8.0; 10% PEG 8000, W/V), then denatured at 98°C for 10 min and incubated at 68°C for 10 h. Subsequently, two samples were mixed together as soon as possible and hybridized at 68°C for 10 h. Hybridized DNA-cDNA duplexes of 1R DNA and cDNA of rye were filled in the ends by using Taq DNA polymerase (TaKaRa, Dalian, 1 U/μl of hybridized DNA) at 72°C for 20 min.

Inverse PCR:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: Paragraph title: Inverse PCR ... A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Ligation:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: .. The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C. .. Next, the ligation reaction was transformed into E . coli cells ( http://tools.invitrogen.com/content/sfs/manuals/gatewayvectorconversion_ccdbsurvival2_man.pdf ).

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL. .. Ligation reactions were incubated at 16 °C for 16 h. For subsequent PCR, 1 μL of digested and re-ligated DNA template was used in a total reaction volume of 25 μL with Tks Gflex DNA Polymerase (TaKaRa, Shiga, Japan).

CpG Methylation Assay:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Polymerase Chain Reaction:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: The cycling parameters were: 94°C for 3 min, followed by 10 cycles of 94°C for 30 s, 64°C for 30 s with a 0.5°C decrement of the annealing temperature per cycle and 72°C for 30 s, followed by 30 cycles of 94°C for 30 s, 59°C for 30 s and 72°C for 30 s, and a final extension step at 72°C for 5 min. After amplification, PCR products were incubated with 2 U shrimp alkaline phosophatase (Fermentas, Vilnius, Lithuania) and 4 U exonuclease I (Fermentas, Vilnius, Lithuania) at 37°C for 1 h, and then denatured at 95°C for 10 min. .. A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China).

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: The reaction was inactivated using the QiaQuick PCR Purification Kit. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Magnetic Beads:

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight. .. The fusion library was immobilized on streptavidin-coated magnetic beads (SA-beads) (Dynabeads MyOne streptavidin C1 streptavidin magnetic beads, Invitrogen, Carlsbad, CA, USA) at 25 °C for 30 min.

Isolation:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C. .. The plasmids of surviving clones were isolated and the direction of the RfB fragment was verified using restriction digests to obtain the binary destination vector pCUbi1390RfB.

Labeling:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: .. A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China). .. LDRs were carried out in a final volume of 20 µL including 9 µL of purified PCR product, 1× Taq DNA ligase buffer, 250 fmol of each LDR primer, 8 U Taq DNA ligase (New England Biolabs, Beverly, Mass, USA).

Purification:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China). .. LDRs were carried out in a final volume of 20 µL including 9 µL of purified PCR product, 1× Taq DNA ligase buffer, 250 fmol of each LDR primer, 8 U Taq DNA ligase (New England Biolabs, Beverly, Mass, USA).

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: .. Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight. .. Translationof the linker-conjugated mRNAs was performed by an in vitro translation system with a Retic Lysate IVT kit (Retic Lysate IVT kit, Ambion, Austin, TX, USA) at 30 °C for 30 min. We did not modify this kit in order to synthesize our peptides composed of four species of amino acid.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: The reaction was inactivated using the QiaQuick PCR Purification Kit. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Sequencing:

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: The restriction enzyme was chosen based on the following criteria: (1) no cutting of the expected breakpoint area; and (2) endonuclease activity would be unaffected by CpG methylation of the target sequence. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Plasmid Preparation:

Article Title: Generation of Wheat Transcription Factor FOX Rice Lines and Systematic Screening for Salt and Osmotic Stress Tolerance
Article Snippet: Paragraph title: Gateway expression vector construction ... The ligation reactions were performed in 10 μl containing 1 μl of lined pCUbi1390 (10 ng), 1 μl of Cassette B (RfB) fragment (50 ng), 1 μl of T4 ligase buffer, and 1 μl of T4 ligase (Takara Bio Inc., Otsu, Japan) overnight at 16°C.

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Software:

Article Title: Polymorphisms on 8q24 Are Associated with Lung Cancer Risk and Survival in Han Chinese
Article Snippet: Primers were designed by software of Primer 3 ( http://frodo.wi.mit.edu/primer3/input.htm ). .. A mixture containing 7.5 pmol of each common FAM labeled LDR primer was phosphorylated in a 15-µl kinase reaction mixture containing 1× T4 ligase buffer and 10 U of T4 kinase (Takara, Dalian, China).

Article Title: Exonic duplication of the OTC gene by a complex rearrangement that likely occurred via a replication-based mechanism: a case report
Article Snippet: ApE – A plasmid Editor software was used to identify the recognition sites for the restriction enzyme. .. A 20 μL sample of digested DNA was then mixed with 23 μL of DW, 5 μL of 10 × T4 ligase buffer (TaKaRa, Shiga, Japan) and 2 μL of T4 DNA ligase to make a final volume of 50 μL.

Selection:

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: Paragraph title: In Vitro Selection against tRNA with a GADV Peptide Library against tRNA ... Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight.

In Vitro:

Article Title: An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
Article Snippet: Paragraph title: In Vitro Selection against tRNA with a GADV Peptide Library against tRNA ... Purified mRNA was hybridized to a short biotin segment puromycin linker (SBP linker) under annealing conditions (heating at 90 °C for 1 min followed by incubation at 70 °C for 1 min and subsequent cooling to 25 °C) in T4 ligase buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2 , 10 mM dithiothreitol, and 1 mM ATP) and ligated by T4 RNA ligase (0.4–2.0 U/pmol mRNA, Takara bio, Otsu, Japan) and polynucleotide kinase (0.5 U/pmol, Toyobo, Osaka, Japan) at 25 °C overnight.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    TaKaRa t4 dna ligase buffer
    Dependence of the efficiency of DNA ligation using <t>T4</t> DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    T4 Dna Ligase Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase buffer/product/TaKaRa
    Average 90 stars, based on 307 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    TaKaRa t4 rna ligase buffer
    Gel electrophoresis pattern of mRNA-linker ligation. The ligation products reacted with or without prRT- DNA oligomer used as a blocker of the 3'-end of mRNA were electrophoresis on 8 M urea 8 % PAGE at 65 °C and were visualized with fluorescence of (A) SYBR Green II and (B) FITC. Lane M: DNA ladder, Lane Y: ligation product, Lane L-: negative control, reaction product without DNA-linker, Lane E-: negative control, reaction product without <t>T4</t> RNA ligase. Mobility of the mRNA-linker and the self-ligation product of mRNA are shown to be equivalent.
    T4 Rna Ligase Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 rna ligase buffer/product/TaKaRa
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t4 rna ligase buffer - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation

    Gel electrophoresis pattern of mRNA-linker ligation. The ligation products reacted with or without prRT- DNA oligomer used as a blocker of the 3'-end of mRNA were electrophoresis on 8 M urea 8 % PAGE at 65 °C and were visualized with fluorescence of (A) SYBR Green II and (B) FITC. Lane M: DNA ladder, Lane Y: ligation product, Lane L-: negative control, reaction product without DNA-linker, Lane E-: negative control, reaction product without T4 RNA ligase. Mobility of the mRNA-linker and the self-ligation product of mRNA are shown to be equivalent.

    Journal: International Journal of Biological Sciences

    Article Title: An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering

    doi:

    Figure Lengend Snippet: Gel electrophoresis pattern of mRNA-linker ligation. The ligation products reacted with or without prRT- DNA oligomer used as a blocker of the 3'-end of mRNA were electrophoresis on 8 M urea 8 % PAGE at 65 °C and were visualized with fluorescence of (A) SYBR Green II and (B) FITC. Lane M: DNA ladder, Lane Y: ligation product, Lane L-: negative control, reaction product without DNA-linker, Lane E-: negative control, reaction product without T4 RNA ligase. Mobility of the mRNA-linker and the self-ligation product of mRNA are shown to be equivalent.

    Article Snippet: Ligation between the mRNA and the hydrazide-linker The mRNA (2 µM) was hybridized to the DNA moiety of the hydrazide-linker (4 µM) and prRT- (4 µM) by heating at 95 °C and cooling to 25 °C in 50 µl of T4 RNA ligase buffer (Takara Bio) and ligation reaction was started by adding T4 RNA ligase (40 U, Takara Bio) and ribonuclease inhibitor (40 U, Takara Bio).

    Techniques: Nucleic Acid Electrophoresis, Ligation, Electrophoresis, Polyacrylamide Gel Electrophoresis, Fluorescence, SYBR Green Assay, Negative Control

    Screening cycle of the mRNA-protein fusion in this study. The dsDNA library is transcribed to mRNA. The mRNA is hybridized to the DNA moiety of the linker having hydrazide group and ligated with T4 RNA ligase. Hydrazide group of the ligated product and acetyl group of the phenylalanine derivative that is acylated to sup tRNA are ligated chemically and the modified mRNA is translated. The modified aminoacyl sup tRNA tends to occupy the A-site of ribosome at UAG codon inserted near downstream of initiation codon and the phenylalanine is incorporated into the growing peptide. Thus, linkage between N-terminus of the nascent peptide and 5'-terminus of its mRNA is achieved. Screening of mRNA-peptide fusion library according to property of the displayed peptide and amplify the genotype molecules of the screened fusions by RT-PCR.

    Journal: International Journal of Biological Sciences

    Article Title: An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering

    doi:

    Figure Lengend Snippet: Screening cycle of the mRNA-protein fusion in this study. The dsDNA library is transcribed to mRNA. The mRNA is hybridized to the DNA moiety of the linker having hydrazide group and ligated with T4 RNA ligase. Hydrazide group of the ligated product and acetyl group of the phenylalanine derivative that is acylated to sup tRNA are ligated chemically and the modified mRNA is translated. The modified aminoacyl sup tRNA tends to occupy the A-site of ribosome at UAG codon inserted near downstream of initiation codon and the phenylalanine is incorporated into the growing peptide. Thus, linkage between N-terminus of the nascent peptide and 5'-terminus of its mRNA is achieved. Screening of mRNA-peptide fusion library according to property of the displayed peptide and amplify the genotype molecules of the screened fusions by RT-PCR.

    Article Snippet: Ligation between the mRNA and the hydrazide-linker The mRNA (2 µM) was hybridized to the DNA moiety of the hydrazide-linker (4 µM) and prRT- (4 µM) by heating at 95 °C and cooling to 25 °C in 50 µl of T4 RNA ligase buffer (Takara Bio) and ligation reaction was started by adding T4 RNA ligase (40 U, Takara Bio) and ribonuclease inhibitor (40 U, Takara Bio).

    Techniques: Modification, Reverse Transcription Polymerase Chain Reaction