t4 dna ligase  (Thermo Fisher)


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    Thermo Fisher t4 dna ligase
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Thermo Fisher
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2022-11
    99/100 stars

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  • 99
    Thermo Fisher t4 dna ligase
    T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2022-11
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher t4 dna ligase 5 u µl
    Experimental procedure, time, and cost of multiple genetic abnormalities sequencing (MGA-Seq). (A) Flowchart of MGA-Seq. Nuclei were cross-linked with 0.5% formaldehyde and then digested with HindIII. 5’ <t>DNA</t> overhangs of digested chromatin fragments were filled in by DNA polymerase and then proximity ligated by <t>T4</t> <t>DNA</t> <t>ligase.</t> The proximity ligation products were fragmented and then subjected to high-throughput sequencing library construction. After sequencing, all the reads were used to generate chromatin contact matrix for genome structural variation calling. In the sequencing library, the reads without ligation junction “AAGCTAGCTT” were used for the detection of CNV, SNP, small indels (
    T4 Dna Ligase 5 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase 5 u µl/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase 5 u µl - by Bioz Stars, 2022-11
    91/100 stars
      Buy from Supplier

    92
    Thermo Fisher enzyme t4 dna ligase
    Experimental procedure, time, and cost of multiple genetic abnormalities sequencing (MGA-Seq). (A) Flowchart of MGA-Seq. Nuclei were cross-linked with 0.5% formaldehyde and then digested with HindIII. 5’ <t>DNA</t> overhangs of digested chromatin fragments were filled in by DNA polymerase and then proximity ligated by <t>T4</t> <t>DNA</t> <t>ligase.</t> The proximity ligation products were fragmented and then subjected to high-throughput sequencing library construction. After sequencing, all the reads were used to generate chromatin contact matrix for genome structural variation calling. In the sequencing library, the reads without ligation junction “AAGCTAGCTT” were used for the detection of CNV, SNP, small indels (
    Enzyme T4 Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme t4 dna ligase/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
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    enzyme t4 dna ligase - by Bioz Stars, 2022-11
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    Experimental procedure, time, and cost of multiple genetic abnormalities sequencing (MGA-Seq). (A) Flowchart of MGA-Seq. Nuclei were cross-linked with 0.5% formaldehyde and then digested with HindIII. 5’ DNA overhangs of digested chromatin fragments were filled in by DNA polymerase and then proximity ligated by T4 DNA ligase. The proximity ligation products were fragmented and then subjected to high-throughput sequencing library construction. After sequencing, all the reads were used to generate chromatin contact matrix for genome structural variation calling. In the sequencing library, the reads without ligation junction “AAGCTAGCTT” were used for the detection of CNV, SNP, small indels (

    Journal: bioRxiv

    Article Title: Robust identification of extrachromosomal DNA and genetic variants using multiple genetic abnormality sequencing (MGA-Seq)

    doi: 10.1101/2022.11.18.517160

    Figure Lengend Snippet: Experimental procedure, time, and cost of multiple genetic abnormalities sequencing (MGA-Seq). (A) Flowchart of MGA-Seq. Nuclei were cross-linked with 0.5% formaldehyde and then digested with HindIII. 5’ DNA overhangs of digested chromatin fragments were filled in by DNA polymerase and then proximity ligated by T4 DNA ligase. The proximity ligation products were fragmented and then subjected to high-throughput sequencing library construction. After sequencing, all the reads were used to generate chromatin contact matrix for genome structural variation calling. In the sequencing library, the reads without ligation junction “AAGCTAGCTT” were used for the detection of CNV, SNP, small indels (

    Article Snippet: In situ proximity ligation Add 27.5 μl of H2O, 3 μl of ATP (adenosine-triphosphate, 10mM), and 10 μl of T4 DNA ligase (Thermo, EL0011) to the reaction system, and placed the tube on the rotating mixers for 2 h at room temperature with rotation at 20 r.p.m.

    Techniques: Sequencing, Ligation, Next-Generation Sequencing