t4 dna ligase  (New England Biolabs)


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    Structured Review

    New England Biolabs t4 dna ligase
    cssDNA productions for three separate inserts. The optimized protocol was tested on the three different plasmids that were constructed (pIS001, pIS002, and pIS003), with inserts shown in the first column. The results of each step of the process for 1 µg of pIS001 (5161 bp), pIS002 (3980 bp), and pIS003 (3867 bp) are shown. Lanes correspond to the steps in the protocol listed in Figure 1 . Lane 0: undigested plasmid <t>DNA</t> containing supercoiled and linearized plasmids. Lane 1a: plasmid is digested with BsaI-HFv2. Lane 1b: Golden Gate Assembly including BsaI-HFv2 and DNA <t>ligase.</t> Lane 2: Cleanup step following Golden Gate assembly. Lanes 3a-b: cssDNA is visible when either the Nt.BbvCI (3a) or Nb.BbvCI (3b) nickase is used for template prep. ssDNA bands do not appear as brightly as dsDNA in part because the SYBR-Safe dye used has a lower quantum yield when bound to ssDNA than dsDNA at the wavelength used for imaging.
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/New England Biolabs
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2022-12
    99/100 stars

    Images

    1) Product Images from "A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation"

    Article Title: A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation

    Journal: bioRxiv

    doi: 10.1101/2022.11.21.517425

    cssDNA productions for three separate inserts. The optimized protocol was tested on the three different plasmids that were constructed (pIS001, pIS002, and pIS003), with inserts shown in the first column. The results of each step of the process for 1 µg of pIS001 (5161 bp), pIS002 (3980 bp), and pIS003 (3867 bp) are shown. Lanes correspond to the steps in the protocol listed in Figure 1 . Lane 0: undigested plasmid DNA containing supercoiled and linearized plasmids. Lane 1a: plasmid is digested with BsaI-HFv2. Lane 1b: Golden Gate Assembly including BsaI-HFv2 and DNA ligase. Lane 2: Cleanup step following Golden Gate assembly. Lanes 3a-b: cssDNA is visible when either the Nt.BbvCI (3a) or Nb.BbvCI (3b) nickase is used for template prep. ssDNA bands do not appear as brightly as dsDNA in part because the SYBR-Safe dye used has a lower quantum yield when bound to ssDNA than dsDNA at the wavelength used for imaging.
    Figure Legend Snippet: cssDNA productions for three separate inserts. The optimized protocol was tested on the three different plasmids that were constructed (pIS001, pIS002, and pIS003), with inserts shown in the first column. The results of each step of the process for 1 µg of pIS001 (5161 bp), pIS002 (3980 bp), and pIS003 (3867 bp) are shown. Lanes correspond to the steps in the protocol listed in Figure 1 . Lane 0: undigested plasmid DNA containing supercoiled and linearized plasmids. Lane 1a: plasmid is digested with BsaI-HFv2. Lane 1b: Golden Gate Assembly including BsaI-HFv2 and DNA ligase. Lane 2: Cleanup step following Golden Gate assembly. Lanes 3a-b: cssDNA is visible when either the Nt.BbvCI (3a) or Nb.BbvCI (3b) nickase is used for template prep. ssDNA bands do not appear as brightly as dsDNA in part because the SYBR-Safe dye used has a lower quantum yield when bound to ssDNA than dsDNA at the wavelength used for imaging.

    Techniques Used: Construct, Plasmid Preparation, Imaging

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    New England Biolabs t4 dna ligase
    T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase/product/New England Biolabs
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase - by Bioz Stars, 2022-12
    99/100 stars
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