t4 dna ligase reaction buffer  (New England Biolabs)


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    Name:
    T4 DNA Ligase Reaction Buffer
    Description:
    T4 DNA Ligase Reaction Buffer 6 0 ml
    Catalog Number:
    b0202s
    Price:
    23
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs t4 dna ligase reaction buffer
    T4 DNA Ligase Reaction Buffer
    T4 DNA Ligase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/t4 dna ligase reaction buffer/product/New England Biolabs
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase reaction buffer - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Ligation:

    Article Title: Lamins organize the global three-dimensional genome from the nuclear periphery
    Article Snippet: Paragraph title: Biotin filling and proximity ligation ... The blunted chromatin DNA was ligated by adding 750 μl 10× T4 ligase buffer (NEB, B0202S), 75 μl 10 mg/ml BSA (NEB), 6151.5 μl distilled water, and 30 μl 30 units/μl T4 DNA ligase (Thermo Scientific, EL0013).

    Article Title: Enhancer architecture and essential core regulatory circuitry of chronic lymphocytic leukemia
    Article Snippet: .. Following this step, a ligation reaction with T4 DNA ligase (NEB #B0202S) was performed O/N, 16°C. .. Next, samples were incubated at 65°C to de-crosslink the chromatin.

    Isolation:

    Article Title: Enhancer architecture and essential core regulatory circuitry of chronic lymphocytic leukemia
    Article Snippet: In brief, nuclei were isolated, and the crosslinked-chromatin was digested overnight (O/N) at 37°C with the primary restriction enzyme. .. Following this step, a ligation reaction with T4 DNA ligase (NEB #B0202S) was performed O/N, 16°C.

    Magnetic Beads:

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: 10X ligase buffer + 10 mM ATP (New England Biolabs, cat. no. B0202S) 100 μM Barcode Adapter (Integrated DNA Technologies) Isopropanol (VWR, cat. no. BDH1133–1LP) !CAUTION Isopropanol is flammable and an irritant. .. Taq DNA Polymerase with Standard Taq Buffer (New England Biolabs, cat. no. M0273L) T4 Polynucleotide Kinase (New England Biolabs, cat. no. M0201L) End Repair Module (New England Biolabs, cat. no. E6050L) Klenow Fragment (3’→5’ exo-) (New England Biolabs, cat. no. M0212L) T4 DNA Ligase (New England Biolabs, cat. no. M0202L) 100 mM dATP (New England Biolabs, cat. no. N0440S) 100 bp DNA Ladder (New England Biolabs, cat. no. N3231S) Rediload (Invitrogen, cat. no. 750026) SeaKem ® ME Agarose (Lonza, cat. no. 50014) 5X Phusion Buffer (New England Biolabs, cat. no. M0530L) Phusion Enzyme (2U/μl) (New England Biolabs, cat. no. M0530L) Solexa Primer 1.0 (10μM) (Integrated DNA Technologies) Solexa Primer 2.0 (10μM) (Integrated DNA Technologies) Thermo Scientific dNTP Set (Thermo Fisher Scientific, cat. no. R0186) Library Quantification Kit - Illumina/ABI Prism (Kapa Biosystems, cat. no. K4835) MiSeq Reagent Kit v3 (Illumina cat. no. MS-102–2023) Seal-Rite 1.5 ml Microcentrifuge Tube (USA Scientific, cat. no. 1615–5500) Glycerol (EMD Millipore Corp., cat. no. 356350–1000ML) RNase A (17,500 U) (Qiagen, cat. no. 19101) E-Gel SizeSelect Agarose Gels, 2% (Invitrogen, cat. no. G661002) GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic (Thermo Fisher Scientific, cat. no. 09981123) Sodium Hydroxide (NaOH; AmericanBio, cat. no. AB01916–050000) !CAUTION Sodium Hydroxide is corrosive.

    Incubation:

    Article Title: Lamins organize the global three-dimensional genome from the nuclear periphery
    Article Snippet: To reverse crosslinking, 25 μl 20 mg/ml proteinase K (Life Technologies, 25530–049) was added and incubated overnight at 65°C. .. The blunted chromatin DNA was ligated by adding 750 μl 10× T4 ligase buffer (NEB, B0202S), 75 μl 10 mg/ml BSA (NEB), 6151.5 μl distilled water, and 30 μl 30 units/μl T4 DNA ligase (Thermo Scientific, EL0013).

    Article Title: Enhancer architecture and essential core regulatory circuitry of chronic lymphocytic leukemia
    Article Snippet: Following this step, a ligation reaction with T4 DNA ligase (NEB #B0202S) was performed O/N, 16°C. .. Next, samples were incubated at 65°C to de-crosslink the chromatin.

    Cell Culture:

    Article Title: Enhancer architecture and essential core regulatory circuitry of chronic lymphocytic leukemia
    Article Snippet: MEC1 cells (~10 million) were harvested from cell culture media, washed 2X in cold PBS, and crosslinked with 1% formaldehyde at room temperature for 10 min. .. Following this step, a ligation reaction with T4 DNA ligase (NEB #B0202S) was performed O/N, 16°C.

    Generated:

    Article Title: Lamins organize the global three-dimensional genome from the nuclear periphery
    Article Snippet: A total of 7.5 ml ligation mixture was incubated overnight at 16°C. .. The blunted chromatin DNA was ligated by adding 750 μl 10× T4 ligase buffer (NEB, B0202S), 75 μl 10 mg/ml BSA (NEB), 6151.5 μl distilled water, and 30 μl 30 units/μl T4 DNA ligase (Thermo Scientific, EL0013).

    Mass Spectrometry:

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: 10X ligase buffer + 10 mM ATP (New England Biolabs, cat. no. B0202S) 100 μM Barcode Adapter (Integrated DNA Technologies) Isopropanol (VWR, cat. no. BDH1133–1LP) !CAUTION Isopropanol is flammable and an irritant. .. Taq DNA Polymerase with Standard Taq Buffer (New England Biolabs, cat. no. M0273L) T4 Polynucleotide Kinase (New England Biolabs, cat. no. M0201L) End Repair Module (New England Biolabs, cat. no. E6050L) Klenow Fragment (3’→5’ exo-) (New England Biolabs, cat. no. M0212L) T4 DNA Ligase (New England Biolabs, cat. no. M0202L) 100 mM dATP (New England Biolabs, cat. no. N0440S) 100 bp DNA Ladder (New England Biolabs, cat. no. N3231S) Rediload (Invitrogen, cat. no. 750026) SeaKem ® ME Agarose (Lonza, cat. no. 50014) 5X Phusion Buffer (New England Biolabs, cat. no. M0530L) Phusion Enzyme (2U/μl) (New England Biolabs, cat. no. M0530L) Solexa Primer 1.0 (10μM) (Integrated DNA Technologies) Solexa Primer 2.0 (10μM) (Integrated DNA Technologies) Thermo Scientific dNTP Set (Thermo Fisher Scientific, cat. no. R0186) Library Quantification Kit - Illumina/ABI Prism (Kapa Biosystems, cat. no. K4835) MiSeq Reagent Kit v3 (Illumina cat. no. MS-102–2023) Seal-Rite 1.5 ml Microcentrifuge Tube (USA Scientific, cat. no. 1615–5500) Glycerol (EMD Millipore Corp., cat. no. 356350–1000ML) RNase A (17,500 U) (Qiagen, cat. no. 19101) E-Gel SizeSelect Agarose Gels, 2% (Invitrogen, cat. no. G661002) GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic (Thermo Fisher Scientific, cat. no. 09981123) Sodium Hydroxide (NaOH; AmericanBio, cat. no. AB01916–050000) !CAUTION Sodium Hydroxide is corrosive.

    Sequencing:

    Article Title: Enhancer architecture and essential core regulatory circuitry of chronic lymphocytic leukemia
    Article Snippet: Libraries for 4C sequencing were prepared as described ( ). .. Following this step, a ligation reaction with T4 DNA ligase (NEB #B0202S) was performed O/N, 16°C.

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  • 90
    New England Biolabs 1x ligation buffer neb b0202
    1x Ligation Buffer Neb B0202, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x ligation buffer neb b0202/product/New England Biolabs
    Average 90 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    1x ligation buffer neb b0202 - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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