t4 dna ligase buffer  (TaKaRa)

 
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    Name:
    T4 DNA Ligase
    Description:
    T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5 phosphate and 3 hydroxyl termini in double stranded DNA using ATP as a coenzyme Both blunt and cohesive end DNA ligation as well as single stranded nick repair of DNA RNA and DNA RNA are possible using the T4 DNA ligase
    Catalog Number:
    2011b
    Price:
    None
    Size:
    125 000 Units
    Category:
    T4 DNA Ligase Ligation enzymes Cloning
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    Structured Review

    TaKaRa t4 dna ligase buffer
    Dependence of the efficiency of DNA ligation using <t>T4</t> DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5 phosphate and 3 hydroxyl termini in double stranded DNA using ATP as a coenzyme Both blunt and cohesive end DNA ligation as well as single stranded nick repair of DNA RNA and DNA RNA are possible using the T4 DNA ligase
    https://www.bioz.com/result/t4 dna ligase buffer/product/TaKaRa
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-03
    99/100 stars

    Images

    1) Product Images from "Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field"

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.10.006

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    Figure Legend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Techniques Used: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.
    Figure Legend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Techniques Used: DNA Ligation, Ligation

    Related Articles

    Fluorescence:

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. The samples prepared by mixing MB solution with 300 nM N2 and N4, as well as the fluorescence measurements were carried out with the addition of variant concentrations of T4 DNA ligase. .. We have developed a simple MB based assay for real-time monitoring of nucleic acids ligation using T4 DNA ligase.

    Synthesized:

    Article Title: Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
    Article Snippet: .. All sequences were synthesized by Takara Biological Engineering Co., Ltd. Other reagents used included dithiothreitol DTT (BBI), T4 DNA ligase (Takara), BSA (Bovogen Biologicals Pty Ltd), protein kinase A (protein kinase A from bovine heart, P5511, Sigma), cAMP (adenosine 3′, 5′-cyclic monophosphate sodium salt monohydrate, 6885, Sigma), ATP (Amresco), kemptide (kemptide acetate salt, K1127, Sigma), genistein (genistein, G6649, Sigma). .. Deionized water was obtained from a Nanopure InfinityTM ultrapure water system (Barnstead/thermolyne Corp, Dubuque, 1A).

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: The downstream RNA including the C to be edited and the 3′ extension of a 15 nt sequence from the KS primer was chemically synthesized by TaKaRa. .. The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight.

    Ligation:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. Two samples containing 10 µl reaction solutions were moved from each sample before adding T4 DNA ligase, and after the ligation process had taken place for 360 s with the addition of ligase. ..

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. Therefore, 37°C was chosen for all ligation reactions to ensure a high sensitivity (the ratio reached 10.7) and a high activity of T4 DNA ligase. .. The ligation process can be affected by many molecular species including biomolecules and metal ions.

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. Figure shows five factors on ligation using T4 DNA ligase. .. Figure A and B shows that the ligation did not take place unless ATP or Mg2+ was added to the ligation mixtures.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. Kinase Assay To explore the ligation mechanism of DNA linkers with 5′-OH ends by T4 DNA ligase, oligo 11 of linker F were phosphorylated by using [γ-32P] ATP and T4 DNA ligase. .. As a result, the phosphorylation products of oligo 11 could be detected by radioautography, suggesting that the 5′-OH of oligo 11 could be phosphorylated by T4 DNA ligase ( ).

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: .. The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight. .. The ligated RNA was purified by PAGE.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers. .. Therefore, it was difficult for us to estimate how many ligation products did not contain these base deletions if these ligation products were not purified with a PCR product purification kit.

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
    Article Snippet: .. In summary, we carried out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on ferromagnetic particles and found that the ligation efficiency was increased under a radio frequency alternating magnetic field caused by heat generation from the particles. .. In the present method, DNA ligase is immobilized on ferromagnetic particles and therefore, if DNA ligation is carried out under moderate temperature conditions, DNA ligase on particles after the reaction may be recovered using a magnet and reused.

    Blocking Assay:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Purification:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: The downstream RNA (300 pmol) was labeled with 32 P at the 5′ end with T4 polynucleotide kinase and purified by passage through a Sephadex G25 spin column (Amersham Pharmacia Biotech). .. The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers. .. Therefore, it was difficult for us to estimate how many ligation products did not contain these base deletions if these ligation products were not purified with a PCR product purification kit.

    Polymerase Chain Reaction:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers. .. Therefore, it was difficult for us to estimate how many ligation products did not contain these base deletions if these ligation products were not purified with a PCR product purification kit.

    Incubation:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    other:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: To check if the phosphorylation of oligo 11 by T4 DNA ligase could be inhibited by CIAP treatment, oligo 11 was treated with CIAP before it was phosphorylated by T4 DNA ligase.

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein
    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: The phosphorylation of oligo 11 by T4 DNA ligase could be inhibited by CIAP treatment of oligo 11.

    Activity Assay:

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. Therefore, 37°C was chosen for all ligation reactions to ensure a high sensitivity (the ratio reached 10.7) and a high activity of T4 DNA ligase. .. The ligation process can be affected by many molecular species including biomolecules and metal ions.

    Labeling:

    Article Title: Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
    Article Snippet: Its 5′-terminal was labeled with a fluorescent group, tetramethyl rhodamine (TAMRA). .. All sequences were synthesized by Takara Biological Engineering Co., Ltd. Other reagents used included dithiothreitol DTT (BBI), T4 DNA ligase (Takara), BSA (Bovogen Biologicals Pty Ltd), protein kinase A (protein kinase A from bovine heart, P5511, Sigma), cAMP (adenosine 3′, 5′-cyclic monophosphate sodium salt monohydrate, 6885, Sigma), ATP (Amresco), kemptide (kemptide acetate salt, K1127, Sigma), genistein (genistein, G6649, Sigma).

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: .. The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight. .. The ligated RNA was purified by PAGE.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight. .. The ligated RNA was purified by PAGE.

    Sequencing:

    Article Title: Involvement of a site-specific trans-acting factor and a common RNA-binding protein in the editing of chloroplast mRNAs: development of a chloroplast in vitro RNA editing system
    Article Snippet: The downstream RNA including the C to be edited and the 3′ extension of a 15 nt sequence from the KS primer was chemically synthesized by TaKaRa. .. The labeled downstream RNA was mixed with the upstream RNA (100 pmol) and the bridge DNA oligonucleotide (200 pmol) (5′-CGGTATCGGATTGTGTCGTAGCTCTATAATTCGGATTAAG3′), and heated at 94°C for 3 min followed by cooling to room temperature for 3 h. Ligation was carried out by adding 1.4 U/µl T4 DNA ligase (TaKaRa) and incubating at 25°C overnight.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers. .. Therefore, it was difficult for us to estimate how many ligation products did not contain these base deletions if these ligation products were not purified with a PCR product purification kit.

    Kinase Assay:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. Kinase Assay To explore the ligation mechanism of DNA linkers with 5′-OH ends by T4 DNA ligase, oligo 11 of linker F were phosphorylated by using [γ-32P] ATP and T4 DNA ligase. .. As a result, the phosphorylation products of oligo 11 could be detected by radioautography, suggesting that the 5′-OH of oligo 11 could be phosphorylated by T4 DNA ligase ( ).

    Variant Assay:

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. The samples prepared by mixing MB solution with 300 nM N2 and N4, as well as the fluorescence measurements were carried out with the addition of variant concentrations of T4 DNA ligase. .. We have developed a simple MB based assay for real-time monitoring of nucleic acids ligation using T4 DNA ligase.

    Concentration Assay:

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
    Article Snippet: .. Note that the inactivation temperature of T4 DNA ligase has been experimentally estimated to be 38 °C, where the inactivation temperature is defined as the temperature at which the concentration of active enzyme becomes half of the total enzyme concentration . .. In the present case, although T4 DNA ligase immobilized on the ferromagnetic particles was heated under the ac magnetic field, the particle's surface temperature, ranging from 20 to 27 °C, was much lower than the above inactivation temperature since the ambient temperature was as low as 16 °C.

    DNA Sequencing:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

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  • 99
    TaKaRa t4 dna ligase buffer
    Dependence of the efficiency of DNA ligation using <t>T4</t> DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    T4 Dna Ligase Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase buffer/product/TaKaRa
    Average 99 stars, based on 307 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation