t4 dna ligase buffer  (TaKaRa)

 
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    Name:
    T4 DNA Ligase
    Description:
    T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5 phosphate and 3 hydroxyl termini in double stranded DNA using ATP as a coenzyme Both blunt and cohesive end DNA ligation as well as single stranded nick repair of DNA RNA and DNA RNA are possible using the T4 DNA ligase
    Catalog Number:
    2011b
    Price:
    None
    Size:
    125 000 Units
    Category:
    T4 DNA Ligase Ligation enzymes Cloning
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    Structured Review

    TaKaRa t4 dna ligase buffer
    Dependence of the efficiency of DNA ligation using <t>T4</t> DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    T4 DNA Ligase is a ligation enzyme that can be used to join DNA fragments by catalyzing the formation of phosphodiester bonds between juxtaposed 5 phosphate and 3 hydroxyl termini in double stranded DNA using ATP as a coenzyme Both blunt and cohesive end DNA ligation as well as single stranded nick repair of DNA RNA and DNA RNA are possible using the T4 DNA ligase
    https://www.bioz.com/result/t4 dna ligase buffer/product/TaKaRa
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase buffer - by Bioz Stars, 2020-08
    99/100 stars

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    1) Product Images from "Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field"

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.10.006

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    Figure Legend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Techniques Used: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.
    Figure Legend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Techniques Used: DNA Ligation, Ligation

    Related Articles

    Synthesized:

    Article Title: Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
    Article Snippet: .. All sequences were synthesized by Takara Biological Engineering Co., Ltd. Other reagents used included dithiothreitol DTT (BBI), T4 DNA ligase (Takara), BSA (Bovogen Biologicals Pty Ltd), protein kinase A (protein kinase A from bovine heart, P5511, Sigma), cAMP (adenosine 3′, 5′-cyclic monophosphate sodium salt monohydrate, 6885, Sigma), ATP (Amresco), kemptide (kemptide acetate salt, K1127, Sigma), genistein (genistein, G6649, Sigma). .. Deionized water was obtained from a Nanopure InfinityTM ultrapure water system (Barnstead/thermolyne Corp, Dubuque, 1A).

    Ligation:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Article Title: Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
    Article Snippet: .. Two samples containing 10 µl reaction solutions were moved from each sample before adding T4 DNA ligase, and after the ligation process had taken place for 360 s with the addition of ligase. ..

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
    Article Snippet: .. In summary, we carried out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on ferromagnetic particles and found that the ligation efficiency was increased under a radio frequency alternating magnetic field caused by heat generation from the particles. .. In the present method, DNA ligase is immobilized on ferromagnetic particles and therefore, if DNA ligation is carried out under moderate temperature conditions, DNA ligase on particles after the reaction may be recovered using a magnet and reused.

    Blocking Assay:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Purification:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Concentration Assay:

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
    Article Snippet: .. Note that the inactivation temperature of T4 DNA ligase has been experimentally estimated to be 38 °C, where the inactivation temperature is defined as the temperature at which the concentration of active enzyme becomes half of the total enzyme concentration . .. In the present case, although T4 DNA ligase immobilized on the ferromagnetic particles was heated under the ac magnetic field, the particle's surface temperature, ranging from 20 to 27 °C, was much lower than the above inactivation temperature since the ambient temperature was as low as 16 °C.

    Incubation:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. These results of CIAP treatments might perhaps give us at least 3 messages: (i) CIAP treatments of linkers A–B could block, but not completely, the ligation of linkers A–B, indicating that some of linkers A–B could still be joined by T4 DNA ligase even after CIAP treatment; (ii) the ligation of linkers A–B could be recovered after CIAP was inactivated at 85°C for 30–90 min, perhaps suggesting that some of linkers A–B could spontaneously delete one or more nucleotide(s) at their 5′-ends and generate some 5′-phosphate ends when the linkers were incubated at 85°C for 30–90 min; and (iii) these results of CIAP treatments again indicated that band 5 in was the ligation products of linkers A–B. .. Three Round Overlap PCR Products and DNA Sequencing To prepare the sequencing template, the ligation products of linkers A–B and C–D were amplified by using 3 round overlap PCR.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    other:

    Article Title: Histone H1 Differentially Inhibits DNA Bending by Reduced and Oxidized HMGB1 Protein
    Article Snippet: In agreement with previous reports [ , ], histone H1 could stimulate formation of linear multimers by T4 DNA ligase at low H1-to-DNA ratios.

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: The phosphorylation of oligo 11 by T4 DNA ligase could be inhibited by CIAP treatment of oligo 11.

    DNA Sequencing:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

    Polymerase Chain Reaction:

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
    Article Snippet: .. We supposed that the reasons might include: (i) CIAP mixture perhaps contained some inhibitors from CIAP or the other components of CIAP mixture; and (ii) an oligo might be hard to be phosphorylated by T4 DNA ligase if it were incubated in CIAP mixture at 85°C for more than 15 min. Our DNA sequencing results demonstrated one or more base deletion(s) at the ligation junction between linkers, but the base deletion background could be significantly reduced if the ligation products of linkers A–B and C–D were purified with a PCR product purification kit before PCR. .. These results might suggest that the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit.

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    TaKaRa t4 dna ligase ferromagnetic particle hybrid dispersed solution
    Dependence of the efficiency of DNA ligation using <t>T4</t> DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.
    T4 Dna Ligase Ferromagnetic Particle Hybrid Dispersed Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase ferromagnetic particle hybrid dispersed solution/product/TaKaRa
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase ferromagnetic particle hybrid dispersed solution - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles in the absence of a magnetic field on the ambient temperature. The ordinate axis represents the ligation efficiency, which is normalized by that at 16 °C. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation

    Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

    doi: 10.1016/j.bbrep.2016.10.006

    Figure Lengend Snippet: Dependence of the efficiency of DNA ligation using T4 DNA ligase immobilized on ferromagnetic particles under an ac magnetic field of 0.34 MHz on the amplitude of the magnetic field. The ambient temperature is 16 °C. The ordinate axis represents the ligation efficiency under an ac magnetic field, which is normalized by that in the absence of a magnetic field. The inset shows the ligation efficiency under the ac magnetic field as a function of the average surface temperature of ferromagnetic particles, noting that the surface temperature increases with an increase in the field amplitude. The standard deviations are obtained from 6 independent experiments.

    Article Snippet: Five μL of T4 DNA ligase/ferromagnetic particle hybrid-dispersed solution, 2 μL of T4 DNA ligase buffer (Takara Bio Inc.), which consisted of 660 mM Tris-HCl (pH 7.6), 66 mM MgCl2 , 100 mM DTT and 1 mM ATP, 5 μL of aqueous solution containing 0.4 mM each of the DNA fragments, and 8 μL of sterilized water were mixed in a test tube, which was placed in a cylindrical container filled with circulating water, the temperature of which was regulated at 16 °C, from a constant-temperature bath (LTB-400, AS ONE CO.).

    Techniques: DNA Ligation, Ligation