Structured Review

Sarstedt t25 flasks
Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flasks/product/Sarstedt
Average 93 stars, based on 26 article reviews
Price from $9.99 to $1999.99
t25 flasks - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "Cell colony counter called CoCoNut"

Article Title: Cell colony counter called CoCoNut

Journal: PLoS ONE

doi: 10.1371/journal.pone.0205823

Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
Figure Legend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

Techniques Used: Cell Culture

2) Product Images from "Cell colony counter called CoCoNut"

Article Title: Cell colony counter called CoCoNut

Journal: PLoS ONE

doi: 10.1371/journal.pone.0205823

Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
Figure Legend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

Techniques Used: Cell Culture

Related Articles

RNA Extraction:

Article Title: A comparative in vitro study of the osteogenic and adipogenic potential of human dental pulp stem cells, gingival fibroblasts and foreskin fibroblasts
Article Snippet: .. Cells were collected from the T25 flasks on day 0 (plating day), 7, 14 and 21 and used for RNA extraction. .. Cells cultured on 24-wells plates were cultured for 21 days, stained (see following paragraph) and examined under a bright-field microscope.

Transfection:

Article Title: Modified Lentiviral LTRs Allow Flp Recombinase–mediated Cassette Exchange and In Vivo Tracing of “Factor-free” Induced Pluripotent Stem Cells
Article Snippet: .. After transfection, cells were cultured in T25 flasks (Sarstedt, Nuembrecht, Germany) with 5 ml of ES medium supplemented with inhibitors for GSK3β and MEK (“2i”). ..

Cell Culture:

Article Title: Distinct Types of Fibrocyte Can Differentiate from Mononuclear Cells in the Presence and Absence of Serum
Article Snippet: .. Dendritic cells (DC) were generated from 2.5×106 CD14+ monocytes resuspended in 5 ml RPMI 1640, 10% pooled human AB+ male serum with recombinant human IL-4 (500 U/ml) and GM-CSF (1000 U/ml; Immunotools, Friesoythe, Germany) and cultured for 6 days in T25 flasks (Sarstedt, Leicester, UK). ..

Article Title: Modified Lentiviral LTRs Allow Flp Recombinase–mediated Cassette Exchange and In Vivo Tracing of “Factor-free” Induced Pluripotent Stem Cells
Article Snippet: .. After transfection, cells were cultured in T25 flasks (Sarstedt, Nuembrecht, Germany) with 5 ml of ES medium supplemented with inhibitors for GSK3β and MEK (“2i”). ..

Article Title: Cell colony counter called CoCoNut
Article Snippet: .. The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively. .. After 7 (V79) or 14 (HeLa) days cells were washed once with 1x PBS before they were fixed and stained with 0.25% - 0.5% (w/v) Crystal Violet (concentration is depending on cell line and quality of the staining solution and should be tested) in methanol for at least 10 min.

Generated:

Article Title: Distinct Types of Fibrocyte Can Differentiate from Mononuclear Cells in the Presence and Absence of Serum
Article Snippet: .. Dendritic cells (DC) were generated from 2.5×106 CD14+ monocytes resuspended in 5 ml RPMI 1640, 10% pooled human AB+ male serum with recombinant human IL-4 (500 U/ml) and GM-CSF (1000 U/ml; Immunotools, Friesoythe, Germany) and cultured for 6 days in T25 flasks (Sarstedt, Leicester, UK). ..

Western Blot:

Article Title: BRAF Mutations Classes I, II, and III in NSCLC Patients Included in the SLLIP Trial: The Need for a New Pre-Clinical Treatment Rationale
Article Snippet: .. Western Blotting For immunoblotting experiments, cells were seeded in T25 flasks (Sarstedt, Newton, NC, USA). .. Cell amounts were 0.5 million per flask (PC9, MDA-MB-231, and H1666), 0.75 million per flask (WM793), after 24 h, cells were either treated with increasing doses of SHP099 (5, 10, and 50 μM), or with increasing doses of trametinib (10, 20, and 50 nM).

Recombinant:

Article Title: Distinct Types of Fibrocyte Can Differentiate from Mononuclear Cells in the Presence and Absence of Serum
Article Snippet: .. Dendritic cells (DC) were generated from 2.5×106 CD14+ monocytes resuspended in 5 ml RPMI 1640, 10% pooled human AB+ male serum with recombinant human IL-4 (500 U/ml) and GM-CSF (1000 U/ml; Immunotools, Friesoythe, Germany) and cultured for 6 days in T25 flasks (Sarstedt, Leicester, UK). ..

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  • 93
    Sarstedt t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Sarstedt
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    86
    Sarstedt • tissue culture flask t25
    <t>T25</t> cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.
    • Tissue Culture Flask T25, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/• tissue culture flask t25/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    • tissue culture flask t25 - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture

    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture

    T25 cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.

    Journal: MethodsX

    Article Title: Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines

    doi: 10.1016/j.mex.2014.11.002

    Figure Lengend Snippet: T25 cardboard cover design for AF calculation method. (a) Shows the cardboard cover design next to T25 flask. (b) and (c) show how cover is placed onto a T25 flask before photography.

    Article Snippet: • Tissue Culture Flask T25, with red vented cap (Sarstedt, Wexford, Ireland).

    Techniques:

    Correlation of AF method with cell counts using a haemocytometer. The average total cell count per T25 flask for each day of a four day growth curve is shown on the x -axis of each graph and the AF output average for the same T25 flask for each day is shown on the y -axis. For each graph error bars represent the standard deviation. (a) Shows a representative graph for OVCAR8. (b) Shows a representative graph for UPN251.

    Journal: MethodsX

    Article Title: Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines

    doi: 10.1016/j.mex.2014.11.002

    Figure Lengend Snippet: Correlation of AF method with cell counts using a haemocytometer. The average total cell count per T25 flask for each day of a four day growth curve is shown on the x -axis of each graph and the AF output average for the same T25 flask for each day is shown on the y -axis. For each graph error bars represent the standard deviation. (a) Shows a representative graph for OVCAR8. (b) Shows a representative graph for UPN251.

    Article Snippet: • Tissue Culture Flask T25, with red vented cap (Sarstedt, Wexford, Ireland).

    Techniques: Cell Counting, Standard Deviation