Structured Review

Becton Dickinson t25 flask
T25 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t25 flask - by Bioz Stars, 2020-04
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Centrifugation:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Immunocytochemistry:

Article Title: Bone marrow-derived endothelial progenitor cells protect postischemic axons after traumatic brain injury
Article Snippet: Paragraph title: Immunocytochemistry And Cellular Labeling ... For the tube-formation assays, EPCs were grown for 8 days in a T25 flask, resuspended, and plated (10,000 cells per well) in Matrigel (BD Biosciences, Mississauga, ON, Canada)-coated 24-well plates.

Cytometry:

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. Immunophenotyping of MSCs was performed by flow cytometry using a FACSCanto II cytometer (BD Biosciences, San Jose, CA, United States) after staining with monoclonal antibodies CD105, CD90, CD73, CD44, HLA-DR, CD34, HLA-ABC, CD19, CD14, and CD45 (all from BD Pharmingen) using standard protocol.

Immunostaining:

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma). .. As judged by GFAP immunostaining, these cultures were > 95% enriched for astrocytes, with remaining cells consisting of microglia and fibroblasts.

Incubation:

Article Title: Bone marrow-derived endothelial progenitor cells protect postischemic axons after traumatic brain injury
Article Snippet: Cells were washed in three changes of PBS between incubation steps and coverslipped with Permafluor (Thermo Scientific). .. For the tube-formation assays, EPCs were grown for 8 days in a T25 flask, resuspended, and plated (10,000 cells per well) in Matrigel (BD Biosciences, Mississauga, ON, Canada)-coated 24-well plates.

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS). ..

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma). .. The astrocyte cell cultures were incubated with medium or with different doses (0.1 to 1 nM) of soluble recombinant HIV-1 IIIB gp120 protein (gp120 IIIB; obtained from the NIH AIDS Research and Reference Reagent Program, Rockville, Md.) for 6 h at 37°C.

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. In vitro T cell activation 1×106 negatively selected naïve CD8+ T cells were incubated with 2.5×107 APC (ACK treated and irradiated spleen cell suspension) in 20 ml OK-DMEM, 10% fetal bovine serum with 10 nM p60217–225 and supplemented with 25 U/ml recombinant IL-2 in T25 flask (Corning 430372 or BD 353081). .. On day 4 post-activation, medium was replaced with fresh media plus 25 U/ml recombinant IL-2 and expanded up to 50 ml in T75 flask (BD 353135).

Expressing:

Article Title: Bone marrow-derived endothelial progenitor cells protect postischemic axons after traumatic brain injury
Article Snippet: Rabbit polyclonal anti-CD133 (1:200) and mouse monoclonal anti-OX-42 (Sigma-Aldrich, 1:500) expression in EPC cultures was used to determine the relative ratio of CD133-expressing EPCs to OX-42-expressing macrophages in EPC cultures. .. For the tube-formation assays, EPCs were grown for 8 days in a T25 flask, resuspended, and plated (10,000 cells per well) in Matrigel (BD Biosciences, Mississauga, ON, Canada)-coated 24-well plates.

Modification:

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: Cells culture, transfection and viruses Human embryonic kidney 293T cells (HEK293T, no. CRL-11268), Human rhabdomyosarcoma RD cells (no. CCL-136) and African green monkey kidney Vero cells (no. CCL-81) were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured as a monolayer in Dulbecco’s modified Eagle’s medium and minimum essential medium (Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated (56 °C, 30 min) fetal calf serum (FCS, GIBCO BRL, Grand Island, NY, USA) and maintained at 37 °C with 5% CO2 in a humidified atmosphere. .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS).

Article Title: Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells
Article Snippet: .. Cells were subsequently cultured in a T25 flask (Falcon®, Becton Dickinson, USA) containing (Dulbecco’s) modified Eagle’s medium (DMEM) high glucose (Gibco) supplemented with 1% penicillin/streptomycin (P/S), 1% amphotericin B, 1% glutamine (Gibco) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD USA) at 37°C, 5% CO2 in order to obtain adherent cells. ..

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. All MSCs were evaluated in their capacity to differentiate into adipocytes, osteocytes, and chondrocytes by using the StemPro Differentiation Kits (Gibco) in accordance with manufacturer’s instructions.

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: .. Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma). .. As judged by GFAP immunostaining, these cultures were > 95% enriched for astrocytes, with remaining cells consisting of microglia and fibroblasts.

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: The hESC line SI-233 was obtained from Stemride International (London, UK, http://www.stemride.com ). hiPSC lines CBiPSC6.2, CBiPSC6.11, and CBiPSC6.13 were derived in our lab from CD34+ cord blood using a modified episomal plasmid methodology described below. hiPSC lines iPSCWT2, and iPSCWT4 were derived in our lab from adult fibroblasts using a modified episomal plasmid methodology described below. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

Derivative Assay:

Article Title: Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells
Article Snippet: Cells were subsequently cultured in a T25 flask (Falcon®, Becton Dickinson, USA) containing (Dulbecco’s) modified Eagle’s medium (DMEM) high glucose (Gibco) supplemented with 1% penicillin/streptomycin (P/S), 1% amphotericin B, 1% glutamine (Gibco) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD USA) at 37°C, 5% CO2 in order to obtain adherent cells. .. Bone marrow derived mesenchymal stem cells (BM-MSCs) were collected by bone marrow (BM) aspirates from three hip surgery patients (60 to 72 years old).

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: Umbilical cord derived MSCs and MenSCs were isolated, characterized, cultured and expanded as previously described ( ; ). .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells.

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: The hESC line SI-233 was obtained from Stemride International (London, UK, http://www.stemride.com ). hiPSC lines CBiPSC6.2, CBiPSC6.11, and CBiPSC6.13 were derived in our lab from CD34+ cord blood using a modified episomal plasmid methodology described below. hiPSC lines iPSCWT2, and iPSCWT4 were derived in our lab from adult fibroblasts using a modified episomal plasmid methodology described below. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

Flow Cytometry:

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. Immunophenotyping of MSCs was performed by flow cytometry using a FACSCanto II cytometer (BD Biosciences, San Jose, CA, United States) after staining with monoclonal antibodies CD105, CD90, CD73, CD44, HLA-DR, CD34, HLA-ABC, CD19, CD14, and CD45 (all from BD Pharmingen) using standard protocol.

Concentration Assay:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. After washes, cells were stained for DNA content by addition of propidium iodide (PI – final concentration 50 μ g ml−1 ) in a volume of 1 ml.

Transferring:

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: Scratches were made with the pipette tip at an angle of around 30° to keep the scratch width limited. .. The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences).

Cell Culture:

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: Cells culture, transfection and viruses Human embryonic kidney 293T cells (HEK293T, no. CRL-11268), Human rhabdomyosarcoma RD cells (no. CCL-136) and African green monkey kidney Vero cells (no. CCL-81) were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured as a monolayer in Dulbecco’s modified Eagle’s medium and minimum essential medium (Hyclone, Logan, UT, USA) supplemented with 10% heat-inactivated (56 °C, 30 min) fetal calf serum (FCS, GIBCO BRL, Grand Island, NY, USA) and maintained at 37 °C with 5% CO2 in a humidified atmosphere. .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS).

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Article Title: Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells
Article Snippet: .. Cells were subsequently cultured in a T25 flask (Falcon®, Becton Dickinson, USA) containing (Dulbecco’s) modified Eagle’s medium (DMEM) high glucose (Gibco) supplemented with 1% penicillin/streptomycin (P/S), 1% amphotericin B, 1% glutamine (Gibco) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD USA) at 37°C, 5% CO2 in order to obtain adherent cells. ..

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: .. The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences). ..

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. All MSCs were evaluated in their capacity to differentiate into adipocytes, osteocytes, and chondrocytes by using the StemPro Differentiation Kits (Gibco) in accordance with manufacturer’s instructions.

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: Paragraph title: Astrocyte cell culture. ... Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma).

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: Pluripotent stem cell lines were initially cultured as colonies on irradiated (5000 cGy) MEF (E13.5 DR4 seeded at 2×104 cells cm−2 ) in 6-well plates (Greiner Bio-One) in hESC medium consisting of DMEM-F12, 15% Knockout Serum Replacer (KSR), 1% non-essential amino acids (NEAA), 100 µM 2-mercaptoethanol and 4 ng mL−1 human FGF2 (R & D Systems, Minneapolis, MN, http://www.rndsystems.com ) and passaged with collagenase IV. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

Imaging:

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: This allowed imaging of both wound edges using the 40× objective lens of a microscope. .. The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences).

Negative Staining:

Article Title: Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury
Article Snippet: After 2 washings in PBS (Lonza), the cell suspension was plated in a T25 flask (Becton Dickinson) in the presence of DMEM (Sigma) and 10% FCS. .. TECs were characterized for their positive staining of cytokeratin, actin, alkaline phosphatase, aminopeptidase A, and megalin, and for negative staining for von Willebrand factor, CD45, nephrin, and desmin.

Molecular Weight:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. After removal of ethanol, 2 × 106 cell aliquots were subjected to treatment with 0.1 M HCl at 37°C for 12 min to extract low molecular weight DNA.

Fluorescence:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

Mutagenesis:

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS). .. At 72 h after post-transfection, cells and supernatants of WT and mutant viruses from transfected HEK293T cells were harvested together, frozen at −80 °C and thawed several times to release the viruses.

Isolation:

Article Title: Characterization of menstrual stem cells: angiogenic effect, migration and hematopoietic stem cell support in comparison with bone marrow mesenchymal stem cells
Article Snippet: Paragraph title: Isolation and culture of cells ... Cells were subsequently cultured in a T25 flask (Falcon®, Becton Dickinson, USA) containing (Dulbecco’s) modified Eagle’s medium (DMEM) high glucose (Gibco) supplemented with 1% penicillin/streptomycin (P/S), 1% amphotericin B, 1% glutamine (Gibco) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD USA) at 37°C, 5% CO2 in order to obtain adherent cells.

Article Title: Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury
Article Snippet: Paragraph title: In vitro Isolation and Expansion of TECs ... After 2 washings in PBS (Lonza), the cell suspension was plated in a T25 flask (Becton Dickinson) in the presence of DMEM (Sigma) and 10% FCS.

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: Umbilical cord derived MSCs and MenSCs were isolated, characterized, cultured and expanded as previously described ( ; ). .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells.

Transfection:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS). ..

Microscopy:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: Once the cells reached ~90% confluence, generally after 72 hours, wound closure in the area was photographed using an inverted phase microscope (CK2, Olympus, Tokyo, Japan) at 40× magnification. .. The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences).

Mouse Assay:

Article Title: Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury
Article Snippet: Kidneys were obtained from healthy female C57 mice. .. After 2 washings in PBS (Lonza), the cell suspension was plated in a T25 flask (Becton Dickinson) in the presence of DMEM (Sigma) and 10% FCS.

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: Primary cultures of cerebral cortical astrocytes were prepared from newborn wild-type or STAT1-null mice as previously described ( ). .. Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma).

Labeling:

Article Title: Bone marrow-derived endothelial progenitor cells protect postischemic axons after traumatic brain injury
Article Snippet: Paragraph title: Immunocytochemistry And Cellular Labeling ... For the tube-formation assays, EPCs were grown for 8 days in a T25 flask, resuspended, and plated (10,000 cells per well) in Matrigel (BD Biosciences, Mississauga, ON, Canada)-coated 24-well plates.

Plasmid Preparation:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: Prior to this, transfection condition was optimized using the pmaxGFPTM vector, provided with the nucleofector kit, according to the manufacturer’s instruction to determine the transfection efficiency and cell viability post transfection. .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining.

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: The hESC line SI-233 was obtained from Stemride International (London, UK, http://www.stemride.com ). hiPSC lines CBiPSC6.2, CBiPSC6.11, and CBiPSC6.13 were derived in our lab from CD34+ cord blood using a modified episomal plasmid methodology described below. hiPSC lines iPSCWT2, and iPSCWT4 were derived in our lab from adult fibroblasts using a modified episomal plasmid methodology described below. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

In Vitro:

Article Title: Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury
Article Snippet: Paragraph title: In vitro Isolation and Expansion of TECs ... After 2 washings in PBS (Lonza), the cell suspension was plated in a T25 flask (Becton Dickinson) in the presence of DMEM (Sigma) and 10% FCS.

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. In vitro T cell activation 1×106 negatively selected naïve CD8+ T cells were incubated with 2.5×107 APC (ACK treated and irradiated spleen cell suspension) in 20 ml OK-DMEM, 10% fetal bovine serum with 10 nM p60217–225 and supplemented with 25 U/ml recombinant IL-2 in T25 flask (Corning 430372 or BD 353081). .. On day 4 post-activation, medium was replaced with fresh media plus 25 U/ml recombinant IL-2 and expanded up to 50 ml in T75 flask (BD 353135).

Irradiation:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: Pluripotent stem cell lines were initially cultured as colonies on irradiated (5000 cGy) MEF (E13.5 DR4 seeded at 2×104 cells cm−2 ) in 6-well plates (Greiner Bio-One) in hESC medium consisting of DMEM-F12, 15% Knockout Serum Replacer (KSR), 1% non-essential amino acids (NEAA), 100 µM 2-mercaptoethanol and 4 ng mL−1 human FGF2 (R & D Systems, Minneapolis, MN, http://www.rndsystems.com ) and passaged with collagenase IV. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. In vitro T cell activation 1×106 negatively selected naïve CD8+ T cells were incubated with 2.5×107 APC (ACK treated and irradiated spleen cell suspension) in 20 ml OK-DMEM, 10% fetal bovine serum with 10 nM p60217–225 and supplemented with 25 U/ml recombinant IL-2 in T25 flask (Corning 430372 or BD 353081). .. On day 4 post-activation, medium was replaced with fresh media plus 25 U/ml recombinant IL-2 and expanded up to 50 ml in T75 flask (BD 353135).

Negative Control:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: Optimization of transfection condition was performed with 6 different NucleofectorTM programs plus 1 negative control (no program). .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining.

Recombinant:

Article Title: Interferon-Independent, Human Immunodeficiency Virus Type 1 gp120-Mediated Induction of CXCL10/IP-10 Gene Expression by Astrocytes In Vivo and In Vitro †
Article Snippet: Supernatants were plated at a density of one brain in three T25 flask (Falcon; Becton Dickinson, Franklin Lakes, N.J.) in Dulbecco's Modified Eagle's medium (Sigma) containing 10% fetal calf serum, l -glutamine, penicillin, and streptomycin (Sigma). .. The astrocyte cell cultures were incubated with medium or with different doses (0.1 to 1 nM) of soluble recombinant HIV-1 IIIB gp120 protein (gp120 IIIB; obtained from the NIH AIDS Research and Reference Reagent Program, Rockville, Md.) for 6 h at 37°C.

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. In vitro T cell activation 1×106 negatively selected naïve CD8+ T cells were incubated with 2.5×107 APC (ACK treated and irradiated spleen cell suspension) in 20 ml OK-DMEM, 10% fetal bovine serum with 10 nM p60217–225 and supplemented with 25 U/ml recombinant IL-2 in T25 flask (Corning 430372 or BD 353081). .. On day 4 post-activation, medium was replaced with fresh media plus 25 U/ml recombinant IL-2 and expanded up to 50 ml in T75 flask (BD 353135).

Wound Healing Assay:

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: Paragraph title: Wound healing assay to confirm wound repair rate ... The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences).

Knock-Out:

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: Pluripotent stem cell lines were initially cultured as colonies on irradiated (5000 cGy) MEF (E13.5 DR4 seeded at 2×104 cells cm−2 ) in 6-well plates (Greiner Bio-One) in hESC medium consisting of DMEM-F12, 15% Knockout Serum Replacer (KSR), 1% non-essential amino acids (NEAA), 100 µM 2-mercaptoethanol and 4 ng mL−1 human FGF2 (R & D Systems, Minneapolis, MN, http://www.rndsystems.com ) and passaged with collagenase IV. .. For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

Activation Assay:

Article Title: Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Article Snippet: .. In vitro T cell activation 1×106 negatively selected naïve CD8+ T cells were incubated with 2.5×107 APC (ACK treated and irradiated spleen cell suspension) in 20 ml OK-DMEM, 10% fetal bovine serum with 10 nM p60217–225 and supplemented with 25 U/ml recombinant IL-2 in T25 flask (Corning 430372 or BD 353081). .. On day 4 post-activation, medium was replaced with fresh media plus 25 U/ml recombinant IL-2 and expanded up to 50 ml in T75 flask (BD 353135).

Staining:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. After washes, cells were stained for DNA content by addition of propidium iodide (PI – final concentration 50 μ g ml−1 ) in a volume of 1 ml.

Article Title: Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular Injury
Article Snippet: After 2 washings in PBS (Lonza), the cell suspension was plated in a T25 flask (Becton Dickinson) in the presence of DMEM (Sigma) and 10% FCS. .. TECs were characterized for their positive staining of cytokeratin, actin, alkaline phosphatase, aminopeptidase A, and megalin, and for negative staining for von Willebrand factor, CD45, nephrin, and desmin.

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. Immunophenotyping of MSCs was performed by flow cytometry using a FACSCanto II cytometer (BD Biosciences, San Jose, CA, United States) after staining with monoclonal antibodies CD105, CD90, CD73, CD44, HLA-DR, CD34, HLA-ABC, CD19, CD14, and CD45 (all from BD Pharmingen) using standard protocol.

Stem Cell Culture:

Article Title: A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability
Article Snippet: Paragraph title: Human pluripotent stem cell culture ... For transfer of hiPSC and hESC to monolayer culture 1 confluent well of a 6-well plate was treated with TrypLE Select and single cells were passaged into a T25 flask (BD Biosciences, Bedford, MA, http://www.bdbiosciences.com ) coated with a 1∶400 dilution (200 µL cm−2 ) of Geltrex in 5 mL of conditioned medium.

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    Becton Dickinson t25 flasks
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Becton Dickinson
    Average 94 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
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    95
    Becton Dickinson t25 flask
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Becton Dickinson
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Full-length amelogenin influences the differentiation of human dental pulp stem cells

    doi: 10.1186/s13287-015-0269-9

    Figure Lengend Snippet: Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Article Snippet: The cells were seeded in T25 flasks (BD Falcon, San Jose, CA, USA), at a density of 2*104 cells/cm2 and cultured in a humidified atmosphere containing 5 % CO2 at 37 °C, with the medium changed twice a week.

    Techniques: Light Microscopy, Cell Culture, Modification