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Becton Dickinson t25 flask
T25 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t25 flask/product/Becton Dickinson
Average 92 stars, based on 20 article reviews
Price from $9.99 to $1999.99
t25 flask - by Bioz Stars, 2020-07
92/100 stars

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Irradiation:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Transfection:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS). ..

Centrifugation:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Cell Culture:

Article Title: Heritability of DNA-damage-induced apoptosis and its relationship with age in lymphocytes from female twins
Article Snippet: .. Apoptotic assay Peripheral blood lymphocytes were separated from 10 ml of blood by centrifugation on Histopaque and 5 × 106 cells diluted in 10 ml RPMI culture medium with 10% serum was added to each T25 flask (Becton Dickinson, Oxford, England) and the cells were cultured for 70 h and either irradiated or mock-treated. .. Irradiation was carried out using a Gammacell 1000 Elite (Nordion International Inc., Ontario Canada) containing a caesium 137 source and with a dose rate of 858 cGy min−1 .

Article Title: Effects of hydrogen-occluding-silica microparticles on wound repair and cell migratory behavior of normal human esophageal epitheliocytes
Article Snippet: .. The wound closure areas were randomly selected so as to be evaluated as statistically significant differences and calculated (mm2 ) in order to show the closure of the wounds at each time period., The second method used a cell scraper (99010, TPP Techno, Trasadingen, Switzerland) for cells cultured in T25 flask (BD Biosciences). ..

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. All MSCs were evaluated in their capacity to differentiate into adipocytes, osteocytes, and chondrocytes by using the StemPro Differentiation Kits (Gibco) in accordance with manufacturer’s instructions.

Incubation:

Article Title: Identification of a nucleotide in 5′ untranslated region contributing to virus replication and virulence of Coxsackievirus A16
Article Snippet: .. A 70% confluent monolayer of HEK293T cells in a T25 flask (BD Biosciences, Franklin Lakes, NJ, USA) was transfected with pcDNA3.1-T7 DNA Pol (1 μg) and CA16 infectious clone (2 μg) or mutants digested by the restriction enzyme Sac I (Takara) using 9 μl Lipofectamine 2000 reagent (Invitrogen) and then incubated at 37 °C in 5 ml of DMEM (10% FCS). ..

Modification:

Article Title: The Reparative Abilities of Menstrual Stem Cells Modulate the Wound Matrix Signals and Improve Cutaneous Regeneration
Article Snippet: .. Cells were collected and cultured in a T25 flask (Falcon® , Becton Dickinson, United States) containing Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 1% penicillin/streptomycin solution (10,000 U/ml / 10,000 μg/ml), 1% amphotericin B (250 μg/ml), 1% L -glutamine (200 mM) (all from Gibco, Paisley, United Kingdom) and 15% fetal bovine serum (FBS) (Lonza, Walkersville, MD, United States) at 37°C, 5% CO2 to obtain adherent cells. .. All MSCs were evaluated in their capacity to differentiate into adipocytes, osteocytes, and chondrocytes by using the StemPro Differentiation Kits (Gibco) in accordance with manufacturer’s instructions.

Staining:

Article Title: The geranyl acetophenone tHGA attenuates human bronchial smooth muscle proliferation via inhibition of AKT phosphorylation
Article Snippet: .. Cells were then seeded in T25 flask (cell viability) and Falcon CultureSlides (8-well) (transfection efficiency) (BD Biosciences, New Jersey, USA) with complete media for 48 hours. hBSMCs in Falcon CultureSlides was fixed with 4% paraformaldehyde followed by Hoechst 33342 DNA staining. .. GFP-expressing hBSMCs were visualized under fluorescence microscope at excitation 488 nm and emission 509 nm while Hoechst stained cells were detected at excitation 352 nm and emission 455 nm.

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  • 92
    Becton Dickinson t25 flasks
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Becton Dickinson
    Average 92 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Becton Dickinson t25 flask
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Becton Dickinson
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Full-length amelogenin influences the differentiation of human dental pulp stem cells

    doi: 10.1186/s13287-015-0269-9

    Figure Lengend Snippet: Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Article Snippet: The cells were seeded in T25 flasks (BD Falcon, San Jose, CA, USA), at a density of 2*104 cells/cm2 and cultured in a humidified atmosphere containing 5 % CO2 at 37 °C, with the medium changed twice a week.

    Techniques: Light Microscopy, Cell Culture, Modification