t25 culture flasks  (Thermo Fisher)


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    Name:
    Chromacol Vial Rack holds 25 vials
    Description:
    Save space in the fridge while ensuring a safe working position on the bench and during transport Available in a various materials colors and capacities Thermo Scientific Chromacol Vial Racks meet a variety of application needs
    Catalog Number:
    t-25
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Chromatography
    Category:
    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher t25 culture flasks
    DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in <t>T25</t> culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope
    Save space in the fridge while ensuring a safe working position on the bench and during transport Available in a various materials colors and capacities Thermo Scientific Chromacol Vial Racks meet a variety of application needs
    https://www.bioz.com/result/t25 culture flasks/product/Thermo Fisher
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    t25 culture flasks - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy"

    Article Title: Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-018-0987-9

    DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope
    Figure Legend Snippet: DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope

    Techniques Used: Infection, Inverted Microscopy

    2) Product Images from "Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy"

    Article Title: Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-018-0987-9

    DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope
    Figure Legend Snippet: DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope

    Techniques Used: Infection, Inverted Microscopy

    Related Articles

    Transfection:

    Article Title: Small Interfering RNAs against the TAR RNA Binding Protein, TRBP, a Dicer Cofactor, Inhibit Human Immunodeficiency Virus Type 1 Long Terminal Repeat Expression and Viral Production ▿
    Article Snippet: .. Transfections of cells with HIV proviral constructs were either in T25 flasks seeded with 7.5 × 105 cells or on six-well plates seeded with 3.0 × 105 cells 24 h prior to cotransfection with siRNA by using Lipofectamine 2000 (Invitrogen). .. To control for transfection efficiency, either pEGFP-N1 (Clontech) was cotransfected as a transfection efficiency reporter and assessed by FACS or experiments were performed at least three times to account for variations.

    Construct:

    Article Title: Small Interfering RNAs against the TAR RNA Binding Protein, TRBP, a Dicer Cofactor, Inhibit Human Immunodeficiency Virus Type 1 Long Terminal Repeat Expression and Viral Production ▿
    Article Snippet: .. Transfections of cells with HIV proviral constructs were either in T25 flasks seeded with 7.5 × 105 cells or on six-well plates seeded with 3.0 × 105 cells 24 h prior to cotransfection with siRNA by using Lipofectamine 2000 (Invitrogen). .. To control for transfection efficiency, either pEGFP-N1 (Clontech) was cotransfected as a transfection efficiency reporter and assessed by FACS or experiments were performed at least three times to account for variations.

    Negative Control:

    Article Title: Heparin regulates B6FS cell motility through a FAK/actin cytoskeleton axis
    Article Snippet: .. All experiments were conducted under serum-free conditions; specifically after 24 h of serum starvation, the cells were treated with UFH and LMWH (10 µ g/ml and 30 µ g/ml) for 48 h. The B6FS cells (450,000 cells per T25 flask) were incubated with siRNA (100 nM) specific for the FAK gene (siFAK), as previously described by Hong et al ( ) and with siRNA negative control sequences (siScramble) for 6 h. Specific RNA (Invitrogen Life Technologies) and Lipofectamine® 2000 (Invitrogen Life Technologies) (1/50 µ l medium) were added to Opti-MEM© (Invitrogen Life Technologies) for 5 min at room temperature. ..

    Knock-Out:

    Article Title: Engineering Efficient Retinal Pigment Epithelium Differentiation From Human Pluripotent Stem Cells
    Article Snippet: .. The cells were seeded onto 0.1% porcine gelatin (Sigma-Aldrich, Gillingham, U.K., ) coated T25 flasks on mitomycin-C inactivated mouse embryo fibroblasts (MEFs) in knockout Dulbecco’s modified Eagle’s medium supplemented with 20% (vol/vol) knockout serum replacement, 2 mM GlutaMAX 1% (vol/vol) nonessential amino acids, 4 ng/ml basic fibroblast growth factor (FGF2) (Invitrogen, Paisley, U.K., ), and 0.1 mM β-mercaptoethanol (Sigma-Aldrich). hESC colonies were passaged every 3–4 days when most colonies were large enough to fill a 10× field of view, and the cells were treated with 0.125 mg/ml collagenase IV (Invitrogen) for 4 minutes at 37°C. .. The colonies with undifferentiated hESC morphology were then dissected into smaller clumps under a dissecting microscope using a fine tip Pasteur pipette.

    Incubation:

    Article Title: Heparin regulates B6FS cell motility through a FAK/actin cytoskeleton axis
    Article Snippet: .. All experiments were conducted under serum-free conditions; specifically after 24 h of serum starvation, the cells were treated with UFH and LMWH (10 µ g/ml and 30 µ g/ml) for 48 h. The B6FS cells (450,000 cells per T25 flask) were incubated with siRNA (100 nM) specific for the FAK gene (siFAK), as previously described by Hong et al ( ) and with siRNA negative control sequences (siScramble) for 6 h. Specific RNA (Invitrogen Life Technologies) and Lipofectamine® 2000 (Invitrogen Life Technologies) (1/50 µ l medium) were added to Opti-MEM© (Invitrogen Life Technologies) for 5 min at room temperature. ..

    Cell Culture:

    Article Title: Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy
    Article Snippet: .. Cell preparation DBTRG cells (a human brain glioblastoma cell line, ATCC number CRL-2020) were seeded in T25 culture flasks at a density of 1 × 106 cells/ml and cultured in RPMI 1640 medium supplemented with 10% FBS, 2 mM GlutaMax, (all from Gibco, Invitrogen). ..

    Article Title: Intrabeam Radiation Inhibits Proliferation, Migration, and Invasiveness and Promotes Apoptosis of MCF-7 Breast Cancer Cells
    Article Snippet: .. To ensure all cells received uniform radiation doses, flat applicators of 60-mm diameter and T-25 cell culture flasks (Thermo Fisher Scientific) were used in this study. .. When applying radiation, the flat applicator was placed upside down and the cell culture flask was placed on the surface of applicator, making the bottom of the flask overlay with the surface of applicator to the extent possible.

    Article Title: Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy
    Article Snippet: .. DBTRG cells (a human brain glioblastoma cell line, ATCC number CRL-2020) were seeded in T25 culture flasks at a density of 1 × 106 cells/ml and cultured in RPMI 1640 medium supplemented with 10% FBS, 2 mM GlutaMax, (all from Gibco, Invitrogen). ..

    Cotransfection:

    Article Title: Small Interfering RNAs against the TAR RNA Binding Protein, TRBP, a Dicer Cofactor, Inhibit Human Immunodeficiency Virus Type 1 Long Terminal Repeat Expression and Viral Production ▿
    Article Snippet: .. Transfections of cells with HIV proviral constructs were either in T25 flasks seeded with 7.5 × 105 cells or on six-well plates seeded with 3.0 × 105 cells 24 h prior to cotransfection with siRNA by using Lipofectamine 2000 (Invitrogen). .. To control for transfection efficiency, either pEGFP-N1 (Clontech) was cotransfected as a transfection efficiency reporter and assessed by FACS or experiments were performed at least three times to account for variations.

    Modification:

    Article Title: Engineering Efficient Retinal Pigment Epithelium Differentiation From Human Pluripotent Stem Cells
    Article Snippet: .. The cells were seeded onto 0.1% porcine gelatin (Sigma-Aldrich, Gillingham, U.K., ) coated T25 flasks on mitomycin-C inactivated mouse embryo fibroblasts (MEFs) in knockout Dulbecco’s modified Eagle’s medium supplemented with 20% (vol/vol) knockout serum replacement, 2 mM GlutaMAX 1% (vol/vol) nonessential amino acids, 4 ng/ml basic fibroblast growth factor (FGF2) (Invitrogen, Paisley, U.K., ), and 0.1 mM β-mercaptoethanol (Sigma-Aldrich). hESC colonies were passaged every 3–4 days when most colonies were large enough to fill a 10× field of view, and the cells were treated with 0.125 mg/ml collagenase IV (Invitrogen) for 4 minutes at 37°C. .. The colonies with undifferentiated hESC morphology were then dissected into smaller clumps under a dissecting microscope using a fine tip Pasteur pipette.

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  • 99
    Thermo Fisher nunclon δ surface t25 cm2 tissue culture flasks
    Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale <t>Nunclon™Δ</t> Surface <t>T25</t> cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.
    Nunclon δ Surface T25 Cm2 Tissue Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunclon δ surface t25 cm2 tissue culture flasks/product/Thermo Fisher
    Average 99 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    nunclon δ surface t25 cm2 tissue culture flasks - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher nunc easyflask cell culture flasks
    Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale <t>Nunclon™Δ</t> Surface <t>T25</t> cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.
    Nunc Easyflask Cell Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc easyflask cell culture flasks/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc easyflask cell culture flasks - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    Image Search Results


    Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale Nunclon™Δ Surface T25 cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Time-course of IL-12p70 production by DCs . IL-12p70 was detected from fresh DCs from 6 h post LPS and IFN-γ stimulation and peaked at 16 h. Fresh replated DCs and thawed replated DCs continued to produce IL-12p70 albeit at a lower level than fresh DCs. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in duplicates in research-scale Nunclon™Δ Surface T25 cm 2 flasks ± SEM. IL-12p70 concentration is expressed as pg/ml.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Concentration Assay

    Mixed leukocyte reaction capability of optimized DCs . DCs generated from clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and DCs generated from research-scale Nunclon™Δ Surface T25 cm 2 flasks using the optimized protocol depicted in Figure 6 were equally potent in stimulating robust mixed leukocytes reactions from 3 different individuals' T cells.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Mixed leukocyte reaction capability of optimized DCs . DCs generated from clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and DCs generated from research-scale Nunclon™Δ Surface T25 cm 2 flasks using the optimized protocol depicted in Figure 6 were equally potent in stimulating robust mixed leukocytes reactions from 3 different individuals' T cells.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Generated

    Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of the effect of Nunclon™Δ Surface, Corning ® cell-culture surface and Corning ® ultra-low attachment surface on DC phenotype and differentiation . DC were cultured with CellGenix DC media containing 2% human AB serum in T25 cm 2 flasks that had the same surface properties as one of the three commercially available cell factories - Nunclon™Δ Surface 1-tray Cell Factories, Corning ® CellSTACK ® culture chambers or Corning ® cell culture ultra-low attachment CellSTACK ® chamber. DC phenotype was evaluated following lysate-loading and maturation for 16 h with LPS and IFN-γ. (A) DCs cultured in Nunclon™Δ Surface flasks (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) exhibited an overall favorable immunophenotype with significantly higher DC-LAMP (** P = 0.003) and lower CD14 (* P = 0.01) when compared to DCs cultured in Corning ® cell-culture surface flasks (surrogate of Corning ® CellSTACK ® culture chambers). Data were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures ± SEM. (B) DCs that were cultured in Corning ® ultra-low attachment surface (surrogate of Corning ® ultra-low attachment cell factory chambers) expressed significantly lower CD86 (** P = 0.011) and HLA-DR (*** P = 0.0004) following lysate-loading and maturation when compared to DCs cultured in Nunclon™Δ Surface (surrogate of Nunclon™Δ Surface 1-tray Cell Factories) and treated the same way. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) in research-scale cultures ± SEM. P values were determined with unpaired Student's t test. * denoted that P values were highly significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Cell Culture

    Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of TrypLE™ Select animal-free tyrpsin immobilization and cold PBS with cell scraping for harvesting mature HOCl-oxidized lysate-loaded DCs . (A) DCs were matured with LPS and IFN-γ for 16 h following lysate-loading. It was observed that mature lysate-loaded DCs that were harvested using TrypLE™ Select animal-free tyrpsin replacement exhibited approximately 30-40% reduction in the levels of MHC Class I ( P = 0.13; NS) and HLA-DR ( P = 0.15; NS) compared to DCs that were harvested with cold DPBS and cell scraping. Data were the mean of 3 independent experiments (i.e. DCs from 3 different individuals) ± SEM in research-scale Nunclon™Δ Surface T25 cm 2 flasks. P values were determined with unpaired Student's t test. NS indicated that P values were not significant. (B) Mature lysate-loaded DCs that were harvested with TrypLE™ Select animal-free tyrpsin replacement were as efficient as DCs that were harvested with cold DPBS and cell scraping in presenting MHC Class I-restricted HER-2/neu and MART-1 peptides and stimulating HER-2/neu and MART-1 CD8 + T cells in intracellular IFN-γ assessments.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques:

    Comparing the phenotype of DCs previously cryopreserved in infusible cryomedia or human AB serum containing 10% DMSO . (A) Middle panel, DCs that had been cyropreserved with infusible cryomedia showed similar expressions of CD86 and CD1c as fresh DCs (first panel) and higher CD80, CD40 and MHC Class I levels after being thawed and replated for 24 h in fresh human AB serum-supplemented CellGenix DC media. Third panel, DCs that were cryopreserved with 90% human AB serum plus 10% DMSO exhibited similar level of CD80, CD40, MHC Class I and CD1c as fresh DCs. DCs cryopreserved in infusible cryomedia expressed approximately 50% lower levels of HLA-DR (** P = 0.001) compared to fresh DCs. On the other hand, DCs cryopreserved in human AB serum plus 10% DMSO media expressed approximately 80% lower levels of HLA-DR (** P = 0.004) compared to fresh DCs. Data were from a clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and was representative of 3 independent research-scales experiments (i.e. DCs from 3 different individuals) in Nunclon™Δ Surface T25 cm 2 flasks. (B) Summary of flow cytometry results from the mean of 3 independent research-scales experiments in Nunclon™Δ Surface T25 cm 2 flasks (MFI ± SEM). P values are determined with unpaired Student's t test. ** denotes P value highly significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparing the phenotype of DCs previously cryopreserved in infusible cryomedia or human AB serum containing 10% DMSO . (A) Middle panel, DCs that had been cyropreserved with infusible cryomedia showed similar expressions of CD86 and CD1c as fresh DCs (first panel) and higher CD80, CD40 and MHC Class I levels after being thawed and replated for 24 h in fresh human AB serum-supplemented CellGenix DC media. Third panel, DCs that were cryopreserved with 90% human AB serum plus 10% DMSO exhibited similar level of CD80, CD40, MHC Class I and CD1c as fresh DCs. DCs cryopreserved in infusible cryomedia expressed approximately 50% lower levels of HLA-DR (** P = 0.001) compared to fresh DCs. On the other hand, DCs cryopreserved in human AB serum plus 10% DMSO media expressed approximately 80% lower levels of HLA-DR (** P = 0.004) compared to fresh DCs. Data were from a clinical-scale Nunclon™Δ Surface 1-tray Cell Factory and was representative of 3 independent research-scales experiments (i.e. DCs from 3 different individuals) in Nunclon™Δ Surface T25 cm 2 flasks. (B) Summary of flow cytometry results from the mean of 3 independent research-scales experiments in Nunclon™Δ Surface T25 cm 2 flasks (MFI ± SEM). P values are determined with unpaired Student's t test. ** denotes P value highly significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Flow Cytometry, Cytometry

    Comparison of the phenotype and IL-12p70 production of mature HOCl-oxidized lysate-loaded DCs generated in human AB serum-free or serum-containing AIM-V and CellGenix DC media, and supplementation with different concentrations of GM-CSF and IL-4 . (A-B) DCs cultured in CellGenix DC media containing 2% human AB serum displayed a desirable phenotype with the highest level of DC-LAMP (* P = 0.005; one-way ANOVA) when compared to DCs cultured in other media conditions, and significantly higher CD86 (* P = 0.05) and lower CD14 (* P = 0.04) when compared to DCs cultured in serum-free CellGenix DC media. (C) DCs generated in CellGenix DC media plus 2% human AB serum produced slightly, though not significantly, higher amount of IL-12p70 compared to DCs generated in other media conditions ( P = 0.2; one-way ANOVA). (D) 500 IU/ml recombinant GM-CSF and 250 IU/ml recombinant IL-4 were sufficient concentrations for generating a favorable immature DC phenotype with low CD14, and intermediate HLA-DR and CD1c. All the data presented were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures in Nunclon™Δ Surface T25 cm 2 flasks ± standard error of the mean (SEM). P values were determined with unpaired Student's t test unless otherwise stated. * denoted that P values were significant.

    Journal: Journal of Translational Medicine

    Article Title: Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate

    doi: 10.1186/1479-5876-9-198

    Figure Lengend Snippet: Comparison of the phenotype and IL-12p70 production of mature HOCl-oxidized lysate-loaded DCs generated in human AB serum-free or serum-containing AIM-V and CellGenix DC media, and supplementation with different concentrations of GM-CSF and IL-4 . (A-B) DCs cultured in CellGenix DC media containing 2% human AB serum displayed a desirable phenotype with the highest level of DC-LAMP (* P = 0.005; one-way ANOVA) when compared to DCs cultured in other media conditions, and significantly higher CD86 (* P = 0.05) and lower CD14 (* P = 0.04) when compared to DCs cultured in serum-free CellGenix DC media. (C) DCs generated in CellGenix DC media plus 2% human AB serum produced slightly, though not significantly, higher amount of IL-12p70 compared to DCs generated in other media conditions ( P = 0.2; one-way ANOVA). (D) 500 IU/ml recombinant GM-CSF and 250 IU/ml recombinant IL-4 were sufficient concentrations for generating a favorable immature DC phenotype with low CD14, and intermediate HLA-DR and CD1c. All the data presented were the mean of 6 independent experiments (i.e. DCs from 6 different individuals) in research-scale cultures in Nunclon™Δ Surface T25 cm 2 flasks ± standard error of the mean (SEM). P values were determined with unpaired Student's t test unless otherwise stated. * denoted that P values were significant.

    Article Snippet: Nunclon™Δ Surface T25 cm2 tissue culture flasks with filter caps and Nunclon™Δ Surface 1-tray Cell Factories were purchased from Thermo Fisher Scientific Inc., Rochester, NY, USA.

    Techniques: Generated, Cell Culture, Produced, Recombinant