t1b 196 cd4 cd70 b cells  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    ATCC t1b 196 cd4 cd70 b cells
    Schematic diagram showing conventional bivalent monospecific <t>anti-CD4</t> and <t>anti-CD70</t> IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.
    T1b 196 Cd4 Cd70 B Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t1b 196 cd4 cd70 b cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t1b 196 cd4 cd70 b cells - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Insights into the molecular basis of a bispecific antibody's target selectivity"

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    Journal: mAbs

    doi: 10.1080/19420862.2015.1022695

    Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.
    Figure Legend Snippet: Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.

    Techniques Used:

    Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Activity Assay, Standard Deviation

     CD4  and  CD70  receptor density on human lymphocytes
    Figure Legend Snippet: CD4 and CD70 receptor density on human lymphocytes

    Techniques Used:

    Binding affinity of IgG and DuetMab to  CD4  and  CD70
    Figure Legend Snippet: Binding affinity of IgG and DuetMab to CD4 and CD70

    Techniques Used: Binding Assay

    Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Standard Deviation

    ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Activity Assay, Standard Deviation

    Cytotoxicity of various  CD4  affinity-reduced DuetMab variants
    Figure Legend Snippet: Cytotoxicity of various CD4 affinity-reduced DuetMab variants

    Techniques Used:

    Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Activity Assay, Concentration Assay, Standard Deviation

    t1b 196 cd4 cd70 b cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    ATCC t1b 196 cd4 cd70 b cells
    Schematic diagram showing conventional bivalent monospecific <t>anti-CD4</t> and <t>anti-CD70</t> IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.
    T1b 196 Cd4 Cd70 B Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t1b 196 cd4 cd70 b cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t1b 196 cd4 cd70 b cells - by Bioz Stars, 2024-04
    93/100 stars

    Images

    1) Product Images from "Insights into the molecular basis of a bispecific antibody's target selectivity"

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    Journal: mAbs

    doi: 10.1080/19420862.2015.1022695

    Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.
    Figure Legend Snippet: Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.

    Techniques Used:

    Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Activity Assay, Standard Deviation

     CD4  and  CD70  receptor density on human lymphocytes
    Figure Legend Snippet: CD4 and CD70 receptor density on human lymphocytes

    Techniques Used:

    Binding affinity of IgG and DuetMab to  CD4  and  CD70
    Figure Legend Snippet: Binding affinity of IgG and DuetMab to CD4 and CD70

    Techniques Used: Binding Assay

    Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Standard Deviation

    ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Activity Assay, Standard Deviation

    Cytotoxicity of various  CD4  affinity-reduced DuetMab variants
    Figure Legend Snippet: Cytotoxicity of various CD4 affinity-reduced DuetMab variants

    Techniques Used:

    Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.
    Figure Legend Snippet: Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Techniques Used: Binding Assay, Activity Assay, Concentration Assay, Standard Deviation

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    ATCC t1b 196 cd4 cd70 b cells
    Schematic diagram showing conventional bivalent monospecific <t>anti-CD4</t> and <t>anti-CD70</t> IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.
    T1b 196 Cd4 Cd70 B Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t1b 196 cd4 cd70 b cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t1b 196 cd4 cd70 b cells - by Bioz Stars, 2024-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques:

    Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques: Binding Assay, Activity Assay, Standard Deviation

     CD4  and  CD70  receptor density on human lymphocytes

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: CD4 and CD70 receptor density on human lymphocytes

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques:

    Binding affinity of IgG and DuetMab to  CD4  and  CD70

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Binding affinity of IgG and DuetMab to CD4 and CD70

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques: Binding Assay

    Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques: Binding Assay, Standard Deviation

    ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques: Activity Assay, Standard Deviation

    Cytotoxicity of various  CD4  affinity-reduced DuetMab variants

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Cytotoxicity of various CD4 affinity-reduced DuetMab variants

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques:

    Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Journal: mAbs

    Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

    doi: 10.1080/19420862.2015.1022695

    Figure Lengend Snippet: Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

    Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

    Techniques: Binding Assay, Activity Assay, Concentration Assay, Standard Deviation