t vincentii  (ATCC)


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    Structured Review

    ATCC t vincentii
    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. <t>vincentii</t> cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    T Vincentii, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    2) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: .. The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: .. Bacterial controls The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Diagnostic Assay:

    Article Title: Enzyme profiles of oral spirochetes in RapID-ANA system.
    Article Snippet: Enzyme profiles of oral Treponema species were determined by using RapID-ANA (Innovative Diagnostic System, Atlanta, Ga.), a 4-h test system which detects 18 enzymatic reactions, including aminopeptidases and glycosidases. .. Seventy-two clinical isolates of Treponema denticola, four reference strains of T. denticola (ATCC 35404, ATCC 35405, ATCC 35520, and ATCC 33521), one strain of T. vincentii (ATCC 35580), and two strains of T. socranskii subspecies (T. socranskii subsp. buccale ATCC 35534 and T. socranskii subsp. socranskii ATCC 35536) were used in this study.

    Negative Control:

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: Bacterial controls The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Produced:

    Article Title: Enzyme profiles of oral spirochetes in RapID-ANA system.
    Article Snippet: Seventy-two clinical isolates of Treponema denticola, four reference strains of T. denticola (ATCC 35404, ATCC 35405, ATCC 35520, and ATCC 33521), one strain of T. vincentii (ATCC 35580), and two strains of T. socranskii subspecies (T. socranskii subsp. buccale ATCC 35534 and T. socranskii subsp. socranskii ATCC 35536) were used in this study. .. All T. denticola strains produced indole and a variety of aminopeptidases and glycosidases.

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    ATCC t vincentii
    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. <t>vincentii</t> cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    T Vincentii, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t vincentii/product/ATCC
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    t vincentii - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    84
    ATCC t vincentii atcc 35580
    Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. <t>vincentii</t> . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, <t>ATCC</t> 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.
    T Vincentii Atcc 35580, supplied by ATCC, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t vincentii atcc 35580/product/ATCC
    Average 84 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t vincentii atcc 35580 - by Bioz Stars, 2020-01
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      Buy from Supplier

    Image Search Results


    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Article Snippet: T. vincentii showed a different coaggregation profile from that of T. denticola ATCC 35405.

    Techniques: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Article Snippet: T. vincentii showed a different coaggregation profile from that of T. denticola ATCC 35405.

    Techniques:

    Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.

    Journal: Infection and Immunity

    Article Title: Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated

    doi:

    Figure Lengend Snippet: Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.

    Article Snippet: Another low-motility mutant, strain LABK4, derived from T. vincentii ATCC 35580, showed the same glycan and H9-2 MAb labeling as its parent (Fig. C and B, lane 4 versus lane 5).

    Techniques: Cell Culture, Isolation, Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling, Immunolabeling, Affinity Purification, Mutagenesis

    Protein and glycan patterns of the type and reference strains of six species of oral treponemes and of three of their motility mutants. Western blots on nitrocellulose after SDS-12.5% PAGE were stained for protein with CPTS (A); after destaining, the same blot was stained for glycan (B) (only the area comprising FlaBs and FlaA, marked in lane 1, is shown; no glycan labeling was detected in other areas of the blot). Lanes 1 to 10 (left to right), respectively: isolated endoflagella of T. amylovorum ATCC 700288 T , T. amylovorum ATCC 700288 T , T. denticola CD-1, T. denticola motility mutant CDK, T. maltophilum ATCC 51939 T , T. pectinovorum ATCC 33768 T , T. pectinovorum motility mutant 33K2, T. socranskii subsp. socranskii ATCC 35536 T , T. socranskii subsp. socranskii motility mutant 447K, and T. vincentii ATCC 35580. The positions of rainbow molecular size standards (Amersham) are indicated in kilodaltons on the right.

    Journal: Infection and Immunity

    Article Title: Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated

    doi:

    Figure Lengend Snippet: Protein and glycan patterns of the type and reference strains of six species of oral treponemes and of three of their motility mutants. Western blots on nitrocellulose after SDS-12.5% PAGE were stained for protein with CPTS (A); after destaining, the same blot was stained for glycan (B) (only the area comprising FlaBs and FlaA, marked in lane 1, is shown; no glycan labeling was detected in other areas of the blot). Lanes 1 to 10 (left to right), respectively: isolated endoflagella of T. amylovorum ATCC 700288 T , T. amylovorum ATCC 700288 T , T. denticola CD-1, T. denticola motility mutant CDK, T. maltophilum ATCC 51939 T , T. pectinovorum ATCC 33768 T , T. pectinovorum motility mutant 33K2, T. socranskii subsp. socranskii ATCC 35536 T , T. socranskii subsp. socranskii motility mutant 447K, and T. vincentii ATCC 35580. The positions of rainbow molecular size standards (Amersham) are indicated in kilodaltons on the right.

    Article Snippet: Another low-motility mutant, strain LABK4, derived from T. vincentii ATCC 35580, showed the same glycan and H9-2 MAb labeling as its parent (Fig. C and B, lane 4 versus lane 5).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling, Isolation, Mutagenesis

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Journal: Journal of Clinical Microbiology

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    doi:

    Figure Lengend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Article Snippet: Table shows that cultivable PROS strains OMZ-802 and OMZ-804 and T. vincentii ATCC 35580 varied in the expression of determinants that cross-reacted with T. pallidum MAb H9-2.

    Techniques: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Journal: Journal of Clinical Microbiology

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    doi:

    Figure Lengend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Article Snippet: Table shows that cultivable PROS strains OMZ-802 and OMZ-804 and T. vincentii ATCC 35580 varied in the expression of determinants that cross-reacted with T. pallidum MAb H9-2.

    Techniques: Polymerase Chain Reaction, Marker