t denticola cells  (ATCC)


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    Name:
    Treponema vincentii LA
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    Catalog Number:
    35580
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    Structured Review

    ATCC t denticola cells
    Antibodies to rMsp inhibit T. <t>denticola</t> cell adhesion.

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    Images

    1) Product Images from "Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405"

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    Journal: Infection and Immunity

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Antibodies to rMsp inhibit T. denticola cell adhesion.
    Figure Legend Snippet: Antibodies to rMsp inhibit T. denticola cell adhesion.

    Techniques Used:

    Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to
    Figure Legend Snippet: Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular
    Figure Legend Snippet: Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular

    Techniques Used: SDS Page, Western Blot

    Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405
    Figure Legend Snippet: Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405

    Techniques Used: Binding Assay, Western Blot, Derivative Assay

    Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with
    Figure Legend Snippet: Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with

    Techniques Used: Recombinant

    Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin
    Figure Legend Snippet: Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin

    Techniques Used:

    2) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    3) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Binding of soluble host proteins to T. denticola cells and proteins. (A) ELISA format. FN, plasminogen (Pgn), or BSA in PBS were added to wells containing immobilized T. denticola ATCC 35405 cells (T.d.), native OppA (OppA), recombinant LacZ-OppA fusion protein (rOppA40), recombinant Msp (rMsp), or BSA. Bound FN and plasminogen were detected with anti-FN (shaded bars) and anti-plasminogen (hatched bars) antibodies, respectively. The y axis shows absorbance at 405 nm. Data shown represent the means ± standard deviations of at least two experiments using triplicate samples. (B) Ligand blot format. Duplicate samples of protein (1 μg) or cells (0.1 ml, OD 600 = 0.2) applied to nitrocellulose membranes were probed with plasminogen (samples 2 to 4) or plasminogen plus EACA (sample 5). Bound plasminogen was detected with anti-plasminogen immunoglobulin G. Lanes: 1, Plasminogen; 2, 5, and 6, T. denticola ATCC 35405; 3, T. denticola OHE; 4, T. denticola MHE.
    Figure Legend Snippet: Binding of soluble host proteins to T. denticola cells and proteins. (A) ELISA format. FN, plasminogen (Pgn), or BSA in PBS were added to wells containing immobilized T. denticola ATCC 35405 cells (T.d.), native OppA (OppA), recombinant LacZ-OppA fusion protein (rOppA40), recombinant Msp (rMsp), or BSA. Bound FN and plasminogen were detected with anti-FN (shaded bars) and anti-plasminogen (hatched bars) antibodies, respectively. The y axis shows absorbance at 405 nm. Data shown represent the means ± standard deviations of at least two experiments using triplicate samples. (B) Ligand blot format. Duplicate samples of protein (1 μg) or cells (0.1 ml, OD 600 = 0.2) applied to nitrocellulose membranes were probed with plasminogen (samples 2 to 4) or plasminogen plus EACA (sample 5). Bound plasminogen was detected with anti-plasminogen immunoglobulin G. Lanes: 1, Plasminogen; 2, 5, and 6, T. denticola ATCC 35405; 3, T. denticola OHE; 4, T. denticola MHE.

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant

    Confirmation of construction of allelic replacement mutants in the opp locus. (A) Southern blots of Sca I-digested T. denticola genomic DNA were probed with a biotinylated internal oppA fragment ( opp ) and the 2.1-kb ermF/AM cassette ( erm ). The ermF/AM cassette contains a single Sca I site. (B) Western immunoblot of T. denticola lysates probed with anti-70 antibodies. Lanes: 1, parent strain ATCC 35405; 2, oppA mutant strain OHE; 3, oppF mutant strain OXE.
    Figure Legend Snippet: Confirmation of construction of allelic replacement mutants in the opp locus. (A) Southern blots of Sca I-digested T. denticola genomic DNA were probed with a biotinylated internal oppA fragment ( opp ) and the 2.1-kb ermF/AM cassette ( erm ). The ermF/AM cassette contains a single Sca I site. (B) Western immunoblot of T. denticola lysates probed with anti-70 antibodies. Lanes: 1, parent strain ATCC 35405; 2, oppA mutant strain OHE; 3, oppF mutant strain OXE.

    Techniques Used: Western Blot, Mutagenesis

    Western immunoblot probed with antibodies raised against recombinant protein rOppA40 (anti-40). Samples were heated at 100°C prior to electrophoresis. Arrows indicate the location of native OppA and rOppA40. Lanes: 1, purified 70-kDa protein; 2, T. denticola whole-cell extract; 3, E. coli pMT1 whole-cell extract.
    Figure Legend Snippet: Western immunoblot probed with antibodies raised against recombinant protein rOppA40 (anti-40). Samples were heated at 100°C prior to electrophoresis. Arrows indicate the location of native OppA and rOppA40. Lanes: 1, purified 70-kDa protein; 2, T. denticola whole-cell extract; 3, E. coli pMT1 whole-cell extract.

    Techniques Used: Western Blot, Recombinant, Electrophoresis, Purification

    Map of the T. denticola opp locus. The 8-kb fragment of T. denticola DNA carried on pMT1 is shown as an open bar, with the locations of some identified restriction enzyme sites shown. Immediately below are arrows representing locations of the genes identified in the opp locus. The solid arrow extending beyond the 8-kb insert represents oppA , while the shaded arrows downstream represent oppB , - C , - D , and - F . Below oppA , the solid arrow represents DNA encoding the 40-kDa LacZ-OppA fusion.
    Figure Legend Snippet: Map of the T. denticola opp locus. The 8-kb fragment of T. denticola DNA carried on pMT1 is shown as an open bar, with the locations of some identified restriction enzyme sites shown. Immediately below are arrows representing locations of the genes identified in the opp locus. The solid arrow extending beyond the 8-kb insert represents oppA , while the shaded arrows downstream represent oppB , - C , - D , and - F . Below oppA , the solid arrow represents DNA encoding the 40-kDa LacZ-OppA fusion.

    Techniques Used:

    ). Residues identical for > 75% of the sequences are outlined. Residues conserved as compared with those of E. coli OppA are shaded. The sequences are presented as follows: protein name:organism/residue numbers. ECOLI, E. coli ; STRPN, Streptococcus pneumoniae ; BACSU, Bacillus subtilis ; SALTY, Salmonella typhimurium ; HAEIN, H. influenzae ; LACLA, Lactococcus lactis ; STRLI, Streptomyces lividans ; EFAEC, Enterococcus faecalis ; BORBU, B. burgdorferi ; TPAL, T. pallidum ; TDENT, T. denticola .
    Figure Legend Snippet: ). Residues identical for > 75% of the sequences are outlined. Residues conserved as compared with those of E. coli OppA are shaded. The sequences are presented as follows: protein name:organism/residue numbers. ECOLI, E. coli ; STRPN, Streptococcus pneumoniae ; BACSU, Bacillus subtilis ; SALTY, Salmonella typhimurium ; HAEIN, H. influenzae ; LACLA, Lactococcus lactis ; STRLI, Streptomyces lividans ; EFAEC, Enterococcus faecalis ; BORBU, B. burgdorferi ; TPAL, T. pallidum ; TDENT, T. denticola .

    Techniques Used:

    Immunogold electron micrograph showing localization of OppA to the cell surface of T. denticola organisms. Spirochete cells were probed with anti-70 antibodies (A) or preimmune rabbit serum (B), and bound antibodies were detected with gold conjugated anti-rabbit immunoglobulins. Bars, 300 nm.
    Figure Legend Snippet: Immunogold electron micrograph showing localization of OppA to the cell surface of T. denticola organisms. Spirochete cells were probed with anti-70 antibodies (A) or preimmune rabbit serum (B), and bound antibodies were detected with gold conjugated anti-rabbit immunoglobulins. Bars, 300 nm.

    Techniques Used:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    4) Product Images from "Oral Spirochetes Implicated in Dental Diseases Are Widespread in Normal Human Subjects and Carry Extremely Diverse Integron Gene Cassettes"

    Article Title: Oral Spirochetes Implicated in Dental Diseases Are Widespread in Normal Human Subjects and Carry Extremely Diverse Integron Gene Cassettes

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00564-12

    Annotation of a contig from sample SRS022602 (SRS022602_Baylor_scaffold_118781) of 3,131 bp. Red diamonds indicate the two repeats identified in this contig with similarity to the attC sites in the T. denticola chromosomal integron, and the three gray
    Figure Legend Snippet: Annotation of a contig from sample SRS022602 (SRS022602_Baylor_scaffold_118781) of 3,131 bp. Red diamonds indicate the two repeats identified in this contig with similarity to the attC sites in the T. denticola chromosomal integron, and the three gray

    Techniques Used:

    5) Product Images from "Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated"

    Article Title: Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated

    Journal: Infection and Immunity

    doi:

    Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.
    Figure Legend Snippet: Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.

    Techniques Used: Cell Culture, Isolation, Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling, Immunolabeling, Affinity Purification, Mutagenesis

    6) Product Images from "Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)"

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00528-18

    Map and immunoblots of deletion mutations made in msp for expression in E. coli . (Upper) The 543-residue Msp protein, with the signal peptide (SP) cleavage site indicated (residue 20), is shown above the deletions made in the coding region. The amino acid residues deleted in the N-terminal region in each of the three constructs are indicated. Deletions in the central region within the approximate boundaries of the central domain (D1 through D2) include predicted antigenic domains (Ag1 and Ag2), the remaining region of greatest divergence between Msps of strains ATCC 35405 and ATCC 33520 (Xr), and D2. These mutant Msps were expressed in E. coli . (Lower) Immunoblots of E. coli strains expressing full-length Msps with the indicated Msp residues deleted are shown. T. denticola ATCC 35405 serves as a positive control. Blots were probed with rabbit polyclonal antibodies raised against native ATCC 35405 Msp, the Msp N-terminal domain, or whole T. denticola (Td) cells. All samples were boiled prior to electrophoresis. Lanes contain lysates of E. coli strains. Antibodies raised against native T. denticola ATCC 35405 Msp or whole T. denticola ATCC 35405 cells do not recognize recombinant Msps lacking residues 229 to 251.
    Figure Legend Snippet: Map and immunoblots of deletion mutations made in msp for expression in E. coli . (Upper) The 543-residue Msp protein, with the signal peptide (SP) cleavage site indicated (residue 20), is shown above the deletions made in the coding region. The amino acid residues deleted in the N-terminal region in each of the three constructs are indicated. Deletions in the central region within the approximate boundaries of the central domain (D1 through D2) include predicted antigenic domains (Ag1 and Ag2), the remaining region of greatest divergence between Msps of strains ATCC 35405 and ATCC 33520 (Xr), and D2. These mutant Msps were expressed in E. coli . (Lower) Immunoblots of E. coli strains expressing full-length Msps with the indicated Msp residues deleted are shown. T. denticola ATCC 35405 serves as a positive control. Blots were probed with rabbit polyclonal antibodies raised against native ATCC 35405 Msp, the Msp N-terminal domain, or whole T. denticola (Td) cells. All samples were boiled prior to electrophoresis. Lanes contain lysates of E. coli strains. Antibodies raised against native T. denticola ATCC 35405 Msp or whole T. denticola ATCC 35405 cells do not recognize recombinant Msps lacking residues 229 to 251.

    Techniques Used: Western Blot, Expressing, Construct, Mutagenesis, Positive Control, Electrophoresis, Recombinant

    7) Product Images from "Multilocus sequence analysis of Treponema denticola strains of diverse origin"

    Article Title: Multilocus sequence analysis of Treponema denticola strains of diverse origin

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-13-24

    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    Figure Legend Snippet: Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.

    Techniques Used:

    Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.
    Figure Legend Snippet: Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.

    Techniques Used: Generated

    8) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    9) Product Images from "Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum"

    Article Title: Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00643-15

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751

    Techniques Used: Binding Assay, Recombinant

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three

    Techniques Used: In Vitro, SDS Page

    10) Product Images from "Multilocus sequence analysis of Treponema denticola strains of diverse origin"

    Article Title: Multilocus sequence analysis of Treponema denticola strains of diverse origin

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-13-24

    Taxonomic resolution based on the ranges of intraspecific sequence similarity (%) for the individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC genes, within the 20 Treponema denticola strains analyzed. The y-axis indicates the levels of nucleotide identity (%) shared between the eight individual gene sequences analyzed from each strain, with the range represented as a bar.
    Figure Legend Snippet: Taxonomic resolution based on the ranges of intraspecific sequence similarity (%) for the individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC genes, within the 20 Treponema denticola strains analyzed. The y-axis indicates the levels of nucleotide identity (%) shared between the eight individual gene sequences analyzed from each strain, with the range represented as a bar.

    Techniques Used: Sequencing

    Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.
    Figure Legend Snippet: Bayesian phylogenetic trees of Treponema denticola strains based on individual 16S rRNA, flaA , recA , pyrH , ppnK , dnaN , era and radC gene datasets. The Bayesian 50% majority-rule consensus tree of 9,000 trees, following the removal of 1,000 trees as burn-in, is shown for each gene. Numbers above branches are posterior probabilities. Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups. The radC gene is absent from the T . pallidum genome.

    Techniques Used:

    Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.
    Figure Legend Snippet: Phylogenetic trees of Treponema denticola strains based on a concatenated 7 - gene dataset ( flaA , recA , pyrH , ppnK , dnaN , era and radC ), using Maximum Likelihood and Bayesian methods. A : Maximum likelihood (ML) tree generated under the GTR + I + G substitution model, with bootstrap values shown above branches. The scale bar represents 0.015 nucleotide changes per site. Numbers beneath the breakpoints in the branches indicate the respective nucleotide changes per site that have been removed. B : Ultrametric Bayesian (BA) 50% majority-rule consensus tree of 9,000 trees following the removal of 1,000 trees as burn-in. Numbers above branches are posterior probabilities. The respective clades formed in each tree are indicated with a Roman numeral (I-VI). Corresponding gene homologoues from Treponema vincentii LA-1 (ATCC 33580) and Treponema pallidum subsp. pallidum SS14 were included in the phylogenetic analysis as outgroups.

    Techniques Used: Generated

    11) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    12) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    13) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    14) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    15) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    16) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    17) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    18) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    19) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    20) Product Images from "Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment"

    Article Title: Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment

    Journal: Infection and Immunity

    doi:

    Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.
    Figure Legend Snippet: Conservation of oppA in oral Treponema strains. (A) Southern blot of Hin dIII-digested chromosomal DNA probed with a 0.9-kb internal fragment of oppA . (B) Western blot of whole-cell extracts probed with anti-40 antibodies. Lanes: 1, T. denticola ATCC 35405; 2, T. denticola ATCC 35404; 3, T. denticola ATCC 33520; 4, T. vincentii LA-1; 5, T. denticola OTK; 6, T. denticola GM-1; 7, T. socranskii ATCC 35536; 8, T. pectinovorum ATCC 33768.

    Techniques Used: Southern Blot, Western Blot

    21) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    22) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿"

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00019-07

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    23) Product Images from "Oral Spirochetes Implicated in Dental Diseases Are Widespread in Normal Human Subjects and Carry Extremely Diverse Integron Gene Cassettes"

    Article Title: Oral Spirochetes Implicated in Dental Diseases Are Widespread in Normal Human Subjects and Carry Extremely Diverse Integron Gene Cassettes

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.00564-12

    A diagram of the constrained assembly approach. (A) Paired-end and singleton reads from a metagenomic data set. (B) Assembly of all reads using SOAPdenovo, to generate contigs and a de Bruijn graph that connects the contigs. (C) Identification of contigs
    Figure Legend Snippet: A diagram of the constrained assembly approach. (A) Paired-end and singleton reads from a metagenomic data set. (B) Assembly of all reads using SOAPdenovo, to generate contigs and a de Bruijn graph that connects the contigs. (C) Identification of contigs

    Techniques Used:

    24) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    25) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    26) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    27) Product Images from "Detection of Novel Integrons in the Metagenome of Human Saliva"

    Article Title: Detection of Novel Integrons in the Metagenome of Human Saliva

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0157605

    The primer binding sites of the published and newly designed primers. Primers were indicated as black arrows on A.) the class 1 integrons and B.) the unusual integron structure of T . denticola . The open arrowed boxes represent ORFs, pointing in the probable direction of transcription. The genes in 5’and 3’ conserved segment (CS), the open reading frame (ORF), the recombination site attI and attC are shown in grey, blue, green, yellow and orange respectively.
    Figure Legend Snippet: The primer binding sites of the published and newly designed primers. Primers were indicated as black arrows on A.) the class 1 integrons and B.) the unusual integron structure of T . denticola . The open arrowed boxes represent ORFs, pointing in the probable direction of transcription. The genes in 5’and 3’ conserved segment (CS), the open reading frame (ORF), the recombination site attI and attC are shown in grey, blue, green, yellow and orange respectively.

    Techniques Used: Binding Assay

    28) Product Images from "Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum"

    Article Title: Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00643-15

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751

    Techniques Used: Binding Assay, Recombinant

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three

    Techniques Used: In Vitro, SDS Page

    29) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    30) Product Images from "Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated"

    Article Title: Flagellins, but Not Endoflagellar Sheath Proteins, of Treponema pallidum and of Pathogen-Related Oral Spirochetes Are Glycosylated

    Journal: Infection and Immunity

    doi:

    Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.
    Figure Legend Snippet: Protein, glycan, and antigen patterns of T. pallidum , cultured PROS, and isolated endoflagella of T. vincentii . Western blots on nitrocellulose after SDS–12% PAGE were stained for protein with CPTS (A). At this stage, the bands of endoflagellar components in lane 2 were marked by pencil to permit accurate reference after removal of the CPTS stain (depending on the intensity of the subsequent glycan labeling or immunolabeling, some of these reference dots may be obscured in panels B to D). After CPTS destaining, the same blot was immunolabeled with MAb H9-2 (B). Parallel blots were stained for glycan (C) or immunolabeled with antibodies affinity purified on the flagellar core protein region of OMZ 803 (D). Lanes 1 to 9 (left to right), respectively: strain ATCC 700013, endoflagella of T. vincentii OMZ 800, T. medium G7201, low-motility mutant LABK4, ATCC 35580 (the parent of LABK4), OMZ 803, RitzA (the parent of OMZ 305A), OMZ 305A, and T. pallidum Nichols. Panels B to D were cut to include only the three bands corresponding to FlaA and the two FlaBs marked in lane 2. The values on the right are molecular masses in kilodaltons.

    Techniques Used: Cell Culture, Isolation, Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling, Immunolabeling, Affinity Purification, Mutagenesis

    Protein and glycan patterns of the type and reference strains of six species of oral treponemes and of three of their motility mutants. Western blots on nitrocellulose after SDS-12.5% PAGE were stained for protein with CPTS (A); after destaining, the same blot was stained for glycan (B) (only the area comprising FlaBs and FlaA, marked in lane 1, is shown; no glycan labeling was detected in other areas of the blot). Lanes 1 to 10 (left to right), respectively: isolated endoflagella of T. amylovorum ATCC 700288 T , T. amylovorum ATCC 700288 T , T. denticola CD-1, T. denticola motility mutant CDK, T. maltophilum ATCC 51939 T , T. pectinovorum ATCC 33768 T , T. pectinovorum motility mutant 33K2, T. socranskii subsp. socranskii ATCC 35536 T , T. socranskii subsp. socranskii motility mutant 447K, and T. vincentii ATCC 35580. The positions of rainbow molecular size standards (Amersham) are indicated in kilodaltons on the right.
    Figure Legend Snippet: Protein and glycan patterns of the type and reference strains of six species of oral treponemes and of three of their motility mutants. Western blots on nitrocellulose after SDS-12.5% PAGE were stained for protein with CPTS (A); after destaining, the same blot was stained for glycan (B) (only the area comprising FlaBs and FlaA, marked in lane 1, is shown; no glycan labeling was detected in other areas of the blot). Lanes 1 to 10 (left to right), respectively: isolated endoflagella of T. amylovorum ATCC 700288 T , T. amylovorum ATCC 700288 T , T. denticola CD-1, T. denticola motility mutant CDK, T. maltophilum ATCC 51939 T , T. pectinovorum ATCC 33768 T , T. pectinovorum motility mutant 33K2, T. socranskii subsp. socranskii ATCC 35536 T , T. socranskii subsp. socranskii motility mutant 447K, and T. vincentii ATCC 35580. The positions of rainbow molecular size standards (Amersham) are indicated in kilodaltons on the right.

    Techniques Used: Western Blot, Polyacrylamide Gel Electrophoresis, Staining, Labeling, Isolation, Mutagenesis

    31) Product Images from "Genetic and Structural Analyses of Cytoplasmic Filaments of Wild-Type Treponema phagedenis and a Flagellar Filament-Deficient Mutant"

    Article Title: Genetic and Structural Analyses of Cytoplasmic Filaments of Wild-Type Treponema phagedenis and a Flagellar Filament-Deficient Mutant

    Journal: Journal of Bacteriology

    doi:

    Identification of the cfpA promoter. (A) Nucleotide sequence identity of promoter regions, including proposed −10 and −35 regions of P cfpA . The arrow indicates the start site of transcription, determined by a primer extension assay with T. phagedenis . RBS, putative ribosome binding site; M, first amino acid of the CfpA polypeptide. Identical nucleotides in the T. phagedenis and T. pallidum subsp. pallidum sequences are indicated by vertical lines. (B) Primer extension assay done to determine the start site of transcription of T. phagedenis cfpA . A, C, G, and T indicate nucleotides used to generate a size ladder with unrelated DNA. Lanes 1 and 2 contain the primer extension reaction products (1 and 5 μl, respectively). nt, nucleotides.
    Figure Legend Snippet: Identification of the cfpA promoter. (A) Nucleotide sequence identity of promoter regions, including proposed −10 and −35 regions of P cfpA . The arrow indicates the start site of transcription, determined by a primer extension assay with T. phagedenis . RBS, putative ribosome binding site; M, first amino acid of the CfpA polypeptide. Identical nucleotides in the T. phagedenis and T. pallidum subsp. pallidum sequences are indicated by vertical lines. (B) Primer extension assay done to determine the start site of transcription of T. phagedenis cfpA . A, C, G, and T indicate nucleotides used to generate a size ladder with unrelated DNA. Lanes 1 and 2 contain the primer extension reaction products (1 and 5 μl, respectively). nt, nucleotides.

    Techniques Used: Sequencing, Primer Extension Assay, Binding Assay

    32) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    33) Product Images from "Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii"

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    Journal: Journal of Clinical Microbiology

    doi:

    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
    Figure Legend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Techniques Used: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .
    Figure Legend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Techniques Used: Polymerase Chain Reaction, Marker

    34) Product Images from "Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum"

    Article Title: Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00643-15

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751

    Techniques Used: Binding Assay, Recombinant

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three

    Techniques Used: In Vitro, SDS Page

    35) Product Images from "Detection of Novel Integrons in the Metagenome of Human Saliva"

    Article Title: Detection of Novel Integrons in the Metagenome of Human Saliva

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0157605

    The primer binding sites of the published and newly designed primers. Primers were indicated as black arrows on A.) the class 1 integrons and B.) the unusual integron structure of T . denticola . The open arrowed boxes represent ORFs, pointing in the probable direction of transcription. The genes in 5’and 3’ conserved segment (CS), the open reading frame (ORF), the recombination site attI and attC are shown in grey, blue, green, yellow and orange respectively.
    Figure Legend Snippet: The primer binding sites of the published and newly designed primers. Primers were indicated as black arrows on A.) the class 1 integrons and B.) the unusual integron structure of T . denticola . The open arrowed boxes represent ORFs, pointing in the probable direction of transcription. The genes in 5’and 3’ conserved segment (CS), the open reading frame (ORF), the recombination site attI and attC are shown in grey, blue, green, yellow and orange respectively.

    Techniques Used: Binding Assay

    36) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    37) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Effect of Treponema bacteria, individually or in combination with P. gingivalis ATCC 33277, on TCT. Equal numbers of Treponema or P. gingivalis cells, either individually or combined, were incubated with human fresh frozen plasma for 5 min at 37 °C
    Figure Legend Snippet: Effect of Treponema bacteria, individually or in combination with P. gingivalis ATCC 33277, on TCT. Equal numbers of Treponema or P. gingivalis cells, either individually or combined, were incubated with human fresh frozen plasma for 5 min at 37 °C

    Techniques Used: Incubation

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    38) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    39) Product Images from "Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405"

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    Journal: Infection and Immunity

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to
    Figure Legend Snippet: Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular
    Figure Legend Snippet: Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular

    Techniques Used: SDS Page, Western Blot

    Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405
    Figure Legend Snippet: Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405

    Techniques Used: Binding Assay, Western Blot, Derivative Assay

    Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with
    Figure Legend Snippet: Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with

    Techniques Used: Recombinant

    Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin
    Figure Legend Snippet: Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin

    Techniques Used:

    40) Product Images from "Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis"

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0133674

    Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for IDD (samples 4567761.3, 4567762.3 and 4567763.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).
    Figure Legend Snippet: Recruitment plot for ADD (samples 4567749.3, 4567750.3, 4567751.3, 4567752.3) mapped on T . denticola ATCC 35405, T . vincentii ATCC 35580 and T . pallidum subsp. pallidum str . Nichols . The blue circle represents the bacterial contigs for the genome of interest, while the two black rings map genes on the forward and reverse strands. Bars represent hits distributions and the colors are coded according to the e-value of the matches with red (-30 and less), orange (-20 to -30), yellow (-10 to -20), green (-5 to -10) and blue (-3 to -5).

    Techniques Used:

    Related Articles

    Mutagenesis:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: .. Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ). .. When treponemes were incubated with fresh human plasma, in combination with equal numbers of P. gingivalis ATCC 33277 cells, TCTs were found to be significantly ( P < 0.01) greater than those observed with each individual species , and were greatest only when CTLP was expressed.

    Sequencing:

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis
    Article Snippet: .. Circular tree of ADD and IDD revealed a high number of sequence reads associated with T . denticola ATCC 35405, T . vincentii ATCC 35580, T .pallidum subsp. Pallidum , T . phagedenis F042 and T . phagedenis ( ). ..

    other:

    Article Title: Shotgun Metagenomic Sequencing Reveals Functional Genes and Microbiome Associated with Bovine Digital Dermatitis
    Article Snippet: The analysis revealed a greater number of sequences with identity to features of T . denticola ATCC 35405 and T . vincentii ATCC 35580 genomes in both ADD and IDD samples.

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    ATCC t vincentii atcc 35580
    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. <t>vincentii</t> ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .
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    RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Journal: Journal of Clinical Microbiology

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    doi:

    Figure Lengend Snippet: RFLP patterns obtained with Rsa I enzyme digests of 16S rDNA PCR products. Lanes: 1 and 12, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium ; 10, DNA from T. phagedenis ; 11, DNA from T. pallidum .

    Article Snippet: The following treponemas were obtained from the American Type Culture Collection (ATCC) unless otherwise noted: T. denticola ATCC 35405, T. denticola ATCC 33521, T. denticola ATCC 35404, T. denticola GM-1 (gift from Denée Thomas, University of Texas, San Antonio), T. phagedenis ATCC 51274, T. vincentii ATCC 35580 and ATCC 700013, T. pectinovorum ATCC 33768, T. socranskii subsp. buccale (ATCC 35534), T. socranskii subsp. paredis (ATCC 35535), T. socranskii subsp. socranskii (ATCC 35536), T. maltophilum ATCC 51939 (gift from Chris Wyss, University of Zurich, Zurich, Switzerland), T. medium G7201 (gift from Toshihiko Umemoto, Asahi University, Asahi, Japan), T. pallidum (D. Thomas), and cultivable PROS isolates OMZ-802, OMZ-804, and OMZ-805 (C. Wyss).

    Techniques: Polymerase Chain Reaction, Marker

    AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Journal: Journal of Clinical Microbiology

    Article Title: Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii

    doi:

    Figure Lengend Snippet: AP-PCR patterns obtained from whole genomic DNA with random primer OPA-2. Lanes: 1 and 10, 1-kb DNA marker (Gibco BRL); 2, DNA from T. vincentii ATCC 35580; 3, DNA from T. vincentii ATCC 700013; 4, DNA from OHSU 242-9; 5, DNA from OHSU 242-10; 6, DNA from OMZ-802; 7, DNA from OMZ-804; 8, DNA from OMZ-805; 9, DNA from T. medium .

    Article Snippet: The following treponemas were obtained from the American Type Culture Collection (ATCC) unless otherwise noted: T. denticola ATCC 35405, T. denticola ATCC 33521, T. denticola ATCC 35404, T. denticola GM-1 (gift from Denée Thomas, University of Texas, San Antonio), T. phagedenis ATCC 51274, T. vincentii ATCC 35580 and ATCC 700013, T. pectinovorum ATCC 33768, T. socranskii subsp. buccale (ATCC 35534), T. socranskii subsp. paredis (ATCC 35535), T. socranskii subsp. socranskii (ATCC 35536), T. maltophilum ATCC 51939 (gift from Chris Wyss, University of Zurich, Zurich, Switzerland), T. medium G7201 (gift from Toshihiko Umemoto, Asahi University, Asahi, Japan), T. pallidum (D. Thomas), and cultivable PROS isolates OMZ-802, OMZ-804, and OMZ-805 (C. Wyss).

    Techniques: Polymerase Chain Reaction, Marker

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Article Snippet: Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ).

    Techniques: