t vincentii  (ATCC)


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    ATCC t vincentii
    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. <t>vincentii</t> cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
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    Images

    1) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    2) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    3) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    4) Product Images from "Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains"

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02499-16

    ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.
    Figure Legend Snippet: ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.

    Techniques Used: Generated, IA

    5) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    6) Product Images from "Detection and Quantification of Oral Treponemes in Subgingival Plaque by Real-Time PCR"

    Article Title: Detection and Quantification of Oral Treponemes in Subgingival Plaque by Real-Time PCR

    Journal:

    doi: 10.1128/JCM.40.9.3334-3340.2002

    Standard curves generated by analysis of a known number of T. denticola (A), T. vincentii (B), and T. medium (C) cells by real-time PCR. Linearity is observed from 103 to 108 cells of these treponemes (correlation coefficients, 0.984 for T. denticola , 0.991 for T. vincentii , and 0.984 for T. medium ).
    Figure Legend Snippet: Standard curves generated by analysis of a known number of T. denticola (A), T. vincentii (B), and T. medium (C) cells by real-time PCR. Linearity is observed from 103 to 108 cells of these treponemes (correlation coefficients, 0.984 for T. denticola , 0.991 for T. vincentii , and 0.984 for T. medium ).

    Techniques Used: Generated, Real-time Polymerase Chain Reaction

    Electrophoresis evaluation of PCR products amplified with primers for T. denticola (A), T. vincentii (B), T. medium (C), and total treponemes (D) and with a ubiquitous primer (E). Lanes: M, molecular size marker (a 100-bp DNA ladder); 1, T. denticola ; 2, T. vincentii ; 3, T. medium ; 4, T. socranskii ; 5, T. phagedenis ; 6, T. pectinovorum ; 7, P. gingivalis ; 8, P. nigrescens ; 9, A. actinomycetemcomitans ; 10, E. coli ; 11, F. nucleatum ; 12, S. mutans ; 13, S. oralis ; and 14, S. salivarius . The expected sizes are noted by arrows.
    Figure Legend Snippet: Electrophoresis evaluation of PCR products amplified with primers for T. denticola (A), T. vincentii (B), T. medium (C), and total treponemes (D) and with a ubiquitous primer (E). Lanes: M, molecular size marker (a 100-bp DNA ladder); 1, T. denticola ; 2, T. vincentii ; 3, T. medium ; 4, T. socranskii ; 5, T. phagedenis ; 6, T. pectinovorum ; 7, P. gingivalis ; 8, P. nigrescens ; 9, A. actinomycetemcomitans ; 10, E. coli ; 11, F. nucleatum ; 12, S. mutans ; 13, S. oralis ; and 14, S. salivarius . The expected sizes are noted by arrows.

    Techniques Used: Electrophoresis, Polymerase Chain Reaction, Amplification, Marker

    7) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    8) Product Images from "Oral Treponemes and Their Outer Membrane Extracts Activate Human Gingival Epithelial Cells through Toll-Like Receptor 2 "

    Article Title: Oral Treponemes and Their Outer Membrane Extracts Activate Human Gingival Epithelial Cells through Toll-Like Receptor 2

    Journal:

    doi: 10.1128/IAI.71.2.717-725.2003

    IL-8 production by HGEC after culturing with oral treponemes. HGEC-1 were cultured with the indicated amounts of T. denticola , T. vincentii , or T. medium in HuMedia-KG2 at 37°C for 6 and 12 h. After incubation, supernatants were collected, and IL-8 production was determined by an ELISA. Experiments were done at least three times, and representative results are presented. Each assay was done with triplicate wells, and the data are expressed as the means ± SEM. Asterisks indicate significant differences seen between groups with and without oral treponemes ( P < 0.01).
    Figure Legend Snippet: IL-8 production by HGEC after culturing with oral treponemes. HGEC-1 were cultured with the indicated amounts of T. denticola , T. vincentii , or T. medium in HuMedia-KG2 at 37°C for 6 and 12 h. After incubation, supernatants were collected, and IL-8 production was determined by an ELISA. Experiments were done at least three times, and representative results are presented. Each assay was done with triplicate wells, and the data are expressed as the means ± SEM. Asterisks indicate significant differences seen between groups with and without oral treponemes ( P < 0.01).

    Techniques Used: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay

    Cytokine mRNA expression and NF-κB activation of HGEC after culturing with oral treponemes. (A) IL-8 and IL-6 mRNAs of HGEC-1 cultured with 5 × 107 cells of oral treponemes for 2 h were analyzed by RT-PCR. β-Actin was assayed as a positive control. PCR products of non-reverse-transcribed (non-RT) samples were examined as a negative control. Lane 1, medium alone; lane 2, T. denticola ; lane 3, T. vincentii ; lane 4, T. medium ; lane M, size marker. Experiments were done at least three times, and representative results are presented. (B) HGEC-1 were transfected with 1 μg of plasmid pNF-κB-Luc and then cultured with the indicated cells of oral treponemes in HuMedia-KG2 at 37°C for 4 h. After incubation, luciferase activity was estimated with a Luminometer. Experiments were done at least three times, and representative results are presented. Each assay was done with triplicate wells, and the data are expressed as the means ± SEM. Asterisks indicate significant differences seen between groups with and without the oral treponemes ( P < 0.01).
    Figure Legend Snippet: Cytokine mRNA expression and NF-κB activation of HGEC after culturing with oral treponemes. (A) IL-8 and IL-6 mRNAs of HGEC-1 cultured with 5 × 107 cells of oral treponemes for 2 h were analyzed by RT-PCR. β-Actin was assayed as a positive control. PCR products of non-reverse-transcribed (non-RT) samples were examined as a negative control. Lane 1, medium alone; lane 2, T. denticola ; lane 3, T. vincentii ; lane 4, T. medium ; lane M, size marker. Experiments were done at least three times, and representative results are presented. (B) HGEC-1 were transfected with 1 μg of plasmid pNF-κB-Luc and then cultured with the indicated cells of oral treponemes in HuMedia-KG2 at 37°C for 4 h. After incubation, luciferase activity was estimated with a Luminometer. Experiments were done at least three times, and representative results are presented. Each assay was done with triplicate wells, and the data are expressed as the means ± SEM. Asterisks indicate significant differences seen between groups with and without the oral treponemes ( P < 0.01).

    Techniques Used: Expressing, Activation Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Positive Control, Polymerase Chain Reaction, Negative Control, Marker, Transfection, Plasmid Preparation, Incubation, Luciferase, Activity Assay

    9) Product Images from "Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains"

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02499-16

    ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.
    Figure Legend Snippet: ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.

    Techniques Used: Generated, IA

    10) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    11) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    12) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    13) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    14) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    15) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    16) Product Images from "Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains"

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02499-16

    ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.
    Figure Legend Snippet: ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.

    Techniques Used: Generated, IA

    17) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    18) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal:

    doi: 10.1099/mic.0.055939-0

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Related Articles

    In Vivo:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: However, T. vincentii showed intermediate adherence levels to Prev. intermedia and Parv. micra , and adherence levels to F. nucleatum similar to those of T. denticola . .. These results provide an in vitro basis for the findings that T. denticola is almost always found associated with P. gingivalis in vivo , while T. vincentii is isolated from subgingival microbial communities ( ; ) but not necessarily associated with P. gingivalis or with periodontal disease. .. Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola .

    Amplification:

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: Riviere et al. previously noted that the Treponema sp. IC strain OMZ 804 (ATCC 700766) could not be amplified by either “ T. vincentii -specific” or “ T. medium -specific” primer sets. .. In addition, the results from these authors' arbitrarily primed PCR analysis of genomic DNA indicated that the OMZ 804 strain was distinct from the T. vincentii ATCC 33580, T. vincentii 802, T. medium G7201, and Treponema sp. IB OMZ 805 strains ( ).

    Zymography:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Confirmatory results were obtained when the abilities of these strains to degrade gelatin were investigated by gelatin zymography. .. This activity was present in extracts of mutant MHE (Msp− ) but absent in extracts from strain CKE (CTLP− ), and from T. vincentii ATCC 35580 ( ).

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed. .. High levels of chymotryspin-like activity were detected in T. denticola ATCC 35405 and T. denticola MHE , whereas no chymotryspin-like activity was detected in T. denticola CKE and T. vincentii ATCC 35580.

    In Vitro:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: However, T. vincentii showed intermediate adherence levels to Prev. intermedia and Parv. micra , and adherence levels to F. nucleatum similar to those of T. denticola . .. These results provide an in vitro basis for the findings that T. denticola is almost always found associated with P. gingivalis in vivo , while T. vincentii is isolated from subgingival microbial communities ( ; ) but not necessarily associated with P. gingivalis or with periodontal disease. .. Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola .

    Polymerase Chain Reaction:

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: Riviere et al. previously noted that the Treponema sp. IC strain OMZ 804 (ATCC 700766) could not be amplified by either “ T. vincentii -specific” or “ T. medium -specific” primer sets. .. In addition, the results from these authors' arbitrarily primed PCR analysis of genomic DNA indicated that the OMZ 804 strain was distinct from the T. vincentii ATCC 33580, T. vincentii 802, T. medium G7201, and Treponema sp. IB OMZ 805 strains ( ). .. These observations are consistent with our MLSA.

    Mutagenesis:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Since T. denticola and P. gingivalis appeared to have synergy in biofilm formation, we examined the dual effects of these bacteria on TCT. .. Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ). .. When treponemes were incubated with fresh human plasma, in combination with equal numbers of P. gingivalis ATCC 33277 cells, TCTs were found to be significantly ( P < 0.01) greater than those observed with each individual species , and were greatest only when CTLP was expressed.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Zymographic analyses revealed that the approximately 95 kDa protein band in T. denticola ATCC 35405 extracts, corresponding to the CTLP complex , had strong gelatinolytic activity ( ). .. This activity was present in extracts of mutant MHE (Msp− ) but absent in extracts from strain CKE (CTLP− ), and from T. vincentii ATCC 35580 ( ). .. Since associations between bacteria are believed to be crucial to the establishment of functional communities , the ability of T. denticola to interact with P. gingivalis , F. nucleatum subsp. vincentii , Prev. intermedia and Parv. micra was determined.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: High levels of chymotryspin-like activity were detected in T. denticola ATCC 35405 and T. denticola MHE , whereas no chymotryspin-like activity was detected in T. denticola CKE and T. vincentii ATCC 35580. .. Zymographic analyses revealed that the approximately 95 kDa protein band in T. denticola ATCC 35405 extracts, corresponding to the CTLP complex , had strong gelatinolytic activity ( ).

    Isolation:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: However, T. vincentii showed intermediate adherence levels to Prev. intermedia and Parv. micra , and adherence levels to F. nucleatum similar to those of T. denticola . .. These results provide an in vitro basis for the findings that T. denticola is almost always found associated with P. gingivalis in vivo , while T. vincentii is isolated from subgingival microbial communities ( ; ) but not necessarily associated with P. gingivalis or with periodontal disease. .. Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola .

    Cell Culture:

    Article Title: Oral Treponemes and Their Outer Membrane Extracts Activate Human Gingival Epithelial Cells through Toll-Like Receptor 2
    Article Snippet: T. denticola ATCC 35404, T. vincentii ATCC 35580, and T. medium ATCC 700293 were grown anaerobically in Trypticase-yeast extract-gelatin-volatile fatty acids-serum (TYGVS) broth containing 5% rabbit serum (Gibco Laboratories, Grand Island, N.Y.) at 37°C for 72 h ( ). .. T. denticola ATCC 35404, T. vincentii ATCC 35580, and T. medium ATCC 700293 were grown anaerobically in Trypticase-yeast extract-gelatin-volatile fatty acids-serum (TYGVS) broth containing 5% rabbit serum (Gibco Laboratories, Grand Island, N.Y.) at 37°C for 72 h ( ).

    Sequencing:

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: With the exception of the recent publication of the genome sequence of the OMZ 838 (ATCC 700772) strain , there is a dearth of genetic or biological information about this proposed species. .. In addition, the results from these authors' arbitrarily primed PCR analysis of genomic DNA indicated that the OMZ 804 strain was distinct from the T. vincentii ATCC 33580, T. vincentii 802, T. medium G7201, and Treponema sp. IB OMZ 805 strains ( ).

    Coagulation:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: It has been hypothesized that CTLP plays an important role in interference with the blood coagulation cascade ( ). .. Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ).

    Activity Assay:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Zymographic analyses revealed that the approximately 95 kDa protein band in T. denticola ATCC 35405 extracts, corresponding to the CTLP complex , had strong gelatinolytic activity ( ). .. This activity was present in extracts of mutant MHE (Msp− ) but absent in extracts from strain CKE (CTLP− ), and from T. vincentii ATCC 35580 ( ). .. Since associations between bacteria are believed to be crucial to the establishment of functional communities , the ability of T. denticola to interact with P. gingivalis , F. nucleatum subsp. vincentii , Prev. intermedia and Parv. micra was determined.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed. .. High levels of chymotryspin-like activity were detected in T. denticola ATCC 35405 and T. denticola MHE , whereas no chymotryspin-like activity was detected in T. denticola CKE and T. vincentii ATCC 35580. .. Confirmatory results were obtained when the abilities of these strains to degrade gelatin were investigated by gelatin zymography.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: The proteolytic activity of the CTLP complex of T. denticola can be conveniently measured using the substrate SAAPFNA ( ). .. Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: The prtP gene is truncated, with the product predicted to lack ~ 160 C-terminal amino acid residues compared with T. denticola PrtP. .. Utilizing a range of protease substrates, were also unable to detect proteolytic activity in T. vincentii . .. Therefore the biological activity and specificity of the analogous T. vincentii CTLP locus remain unclear.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: However, adherence was not ablated, suggesting other contributory factors. .. T. vincentii , which contains an incomplete CTLP gene locus and does not express chymotrypsin-like activity, adhered strongly to F. nucleatum , weakly to Prev. intermedia and Parv. micra , and failed to adhere to P. gingivalis ( ). .. Since CTLP expression was found to be necessary for coadhesion, we investigated whether enzymic activity per se was required for the adherence process.

    Microscopy:

    Article Title: Detection and Quantification of Oral Treponemes in Subgingival Plaque by Real-Time PCR
    Article Snippet: T. denticola strain ATCC 35404, T. vincentii ATCC 35580, and T. medium ATCC 700293 were grown anaerobically in trypticase-yeast extract-gelatine-volatile fatty acid-rabbit serum broth containing 5% rabbit serum at 37°C for 72 h ( ). .. T. denticola strain ATCC 35404, T. vincentii ATCC 35580, and T. medium ATCC 700293 were grown anaerobically in trypticase-yeast extract-gelatine-volatile fatty acid-rabbit serum broth containing 5% rabbit serum at 37°C for 72 h ( ).

    IA:

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: As noted above, T. sinensis ( Treponema sp. IA) has been proposed to constitute a distinct putative “species” of oral treponeme (Wyss, unpublished). .. In addition, the results from these authors' arbitrarily primed PCR analysis of genomic DNA indicated that the OMZ 804 strain was distinct from the T. vincentii ATCC 33580, T. vincentii 802, T. medium G7201, and Treponema sp. IB OMZ 805 strains ( ).

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: Taken together, the results from our multilocus phylogenetic and sequence-similarity-based analyses of oral treponeme strains clearly indicate that the 16S rRNA, flaA , recA , and pyrH genes can be used to effectively characterize and discriminate phylogroup 1 and 2 taxa spanning multiple species. .. Our data confirm the taxonomic positions of T. vincentii , T. medium , and T. putidum and defines three new “species-level” phylotypes: T. sinensis ( Treponema sp. IA), Treponema sp. IB, and Treponema sp. IC. .. We envisage that results from future whole-genome-based analyses and systematic polyphasic taxonomic studies will support the oral treponeme “species” definitions proposed here.

    other:

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains
    Article Snippet: This phenotypic property is responsible for the ability of several strains of T. denticola , T. putidum , T. medium , T. vincentii , and “ T. vincentii -like” species to cross-react with monoclonal antibodies (e.g., H9-2) raised against T. pallidum .

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: T. vincentii carries an incomplete CTLP gene locus.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: However, T. vincentii showed intermediate adherence levels to Prev. intermedia and Parv. micra , and adherence levels to F. nucleatum similar to those of T. denticola .

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: The strain of T. vincentii used in this work was not able to adhere to P. gingivalis , in keeping with the notion that CTLP is important in this interaction.

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola .

    Article Title: Molecular Signaling Mechanisms of the Periopathogen, Treponema denticola
    Article Snippet: T. vincentii ATCC 35580 and T. lecithinolyticum OMZ684T harbor 2.51-Mb (2559 ORFs) and 1.47-Mb (2059 ORFs) chromosomes, respectively ( ).

    Migration:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities
    Article Snippet: Overall, T. vincentii appeared to have a less adherent phenotype than T. denticola . .. However, T. vincentii shows greater motility than T. denticola in migration experiments through semisolid medium ( ). .. Thus it might be speculated that while T. denticola shows firmer adherence, the greater motility of T. vincentii facilitates movement into tissues.

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    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. <t>vincentii</t> cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
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    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Journal:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Article Snippet: Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ).

    Techniques: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Journal:

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Article Snippet: Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ).

    Techniques:

    ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.

    Journal: Applied and Environmental Microbiology

    Article Title: Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains

    doi: 10.1128/AEM.02499-16

    Figure Lengend Snippet: ML phylogenetic tree of oral treponeme 16S rRNA genes. The maximum clade credibility tree topology is supported by bootstrapping for 1,000 replicates (first number) and Bayesian posterior probabilities (second number) separated with a forward slash “/” symbol in percentages; only values over 40% are shown beside the branch nodes. Sequences generated in this study are in boldface type. A scale bar indicates the nucleotide changes per position. Excessively long branches were trimmed in proportion to the scale bar (indicated by “//” on the branches). The respective oral treponeme species (phylogroups) are indicated with different color shadings. The corresponding HOT numbers are also indicated as follows: T. denticola (HOT 584), dark blue; T. putidum (HOT 743), red; T. vincentii (HOT 029), light green; Treponema sp. IA (HOT 231), purple; Treponema sp. IB (HOT 667), light blue; and Treponema sp. IC (HOT 231), orange.

    Article Snippet: This phenotypic property is responsible for the ability of several strains of T. denticola , T. putidum , T. medium , T. vincentii , and “ T. vincentii -like” species to cross-react with monoclonal antibodies (e.g., H9-2) raised against T. pallidum .

    Techniques: Generated, IA