n a cplc6144 c parapsilosis wild type strain ashford35 atcc 22019 trlc8674 t rubrum clinical  (ATCC)

Journal: Pharmaceutics

Article Title: Bioinformatics-Driven mRNA-Based Vaccine Design for Controlling Tinea Cruris Induced by Trichophyton rubrum

doi: 10.3390/pharmaceutics16080983

Figure Lengend Snippet: Physiochemical properties of sorted proteins from T. rubrum targeted for vaccine design.

Article Snippet: Specifically, we utilized the T. rubrum strain ATCC MYA-4607/CBS 118892, corresponding to UniProt Proteome identifier UP000008864, which contains 10,006 coding protein sequences.

Techniques: In Vitro, In Vivo, Solubility, Expressing

Journal: Pharmaceutics

Article Title: Bioinformatics-Driven mRNA-Based Vaccine Design for Controlling Tinea Cruris Induced by Trichophyton rubrum

doi: 10.3390/pharmaceutics16080983

Figure Lengend Snippet: NetMHCpan EL 4.1 method on the IEDB server predicted antigenic CTL binding epitopes of sorted proteins from T. rubrum targeted for vaccine design.

Article Snippet: Specifically, we utilized the T. rubrum strain ATCC MYA-4607/CBS 118892, corresponding to UniProt Proteome identifier UP000008864, which contains 10,006 coding protein sequences.

Techniques: Binding Assay

Journal: Pharmaceutics

Article Title: Bioinformatics-Driven mRNA-Based Vaccine Design for Controlling Tinea Cruris Induced by Trichophyton rubrum

doi: 10.3390/pharmaceutics16080983

Figure Lengend Snippet: Antigenic HTL binding epitopes of sorted proteins from T. rubrum targeted for vaccine design, predicted using IEDB recommended 2.22 method on the IEDB server.

Article Snippet: Specifically, we utilized the T. rubrum strain ATCC MYA-4607/CBS 118892, corresponding to UniProt Proteome identifier UP000008864, which contains 10,006 coding protein sequences.

Techniques: Binding Assay

Journal: Pharmaceutics

Article Title: Bioinformatics-Driven mRNA-Based Vaccine Design for Controlling Tinea Cruris Induced by Trichophyton rubrum

doi: 10.3390/pharmaceutics16080983

Figure Lengend Snippet: Predicted sorted protein LBL epitopes of T. rubrum using IEDB’s BepiPred Linear Epitope Prediction 2.0 methods.

Article Snippet: Specifically, we utilized the T. rubrum strain ATCC MYA-4607/CBS 118892, corresponding to UniProt Proteome identifier UP000008864, which contains 10,006 coding protein sequences.

Techniques:

Journal: Pharmaceutics

Article Title: Bioinformatics-Driven mRNA-Based Vaccine Design for Controlling Tinea Cruris Induced by Trichophyton rubrum

doi: 10.3390/pharmaceutics16080983

Figure Lengend Snippet: Characteristic features of BGTV, CDPV, GMPV, and LDPV candidate docking complexes against T. rubrum and immune receptors (TLR2 and TLR4).

Article Snippet: Specifically, we utilized the T. rubrum strain ATCC MYA-4607/CBS 118892, corresponding to UniProt Proteome identifier UP000008864, which contains 10,006 coding protein sequences.

Techniques: Binding Assay

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Oligonucleotide primers were used to evaluate the relative gene expression of T. rubrum ATCC 28189, treated with nonyl protocatechuate, and compared to the untreated fungus.

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques: Expressing

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Scanning electron microscopy of T. rubrum and T. mentagrophytes mature biofilms, untreated ( A , F , K , P ) and treated with fluconazole ( B , G , L , Q ); griseofulvin ( C , H , M , R ); terbinafine ( D , I , N , S ) and nonyl 3,4-dihydroxybenzoate ( E , J , O , T ). White arrows denote small structures, similar to atrophied microconidia or extracellular vesicles ( E , J ).

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques: Electron Microscopy

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Orthogonal sections obtained by confocal laser scanning microscopy of mature T. rubrum and T. mentagrophytes biofilms, untreated ( A , D , G , J ); treated with terbinafine 32 mg/L ( B , E , H , K ) and treated with nonyl 3, 4-dihydroxybenzoate 250 mg/L ( C , F , I , L ), showed that both treatments reduced the thickness and/or mass of biofilms. Pictures were taken at 63× magnification, 20 µm bars. Metabolically active cells are stained red (FUN 1), while cells surrounded by polysaccharide material are stained green (ConA). The image has been adjusted for brightness.

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques: Confocal Laser Scanning Microscopy, Metabolic Labelling, Staining

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Confocal laser scanning microscopy images of untreated T. rubrum ATCC 28189 ( A ), treated with 7.8 mg/L nonyl ( B ), 128 mg/L fluconazole ( C ), and 1 mg/L amphotericin B ( D ) at a magnification of 100×; bars, 10 µm. The panels show cell walls stained with calcofluor white, even in those in which more extensive damage and inhibition of hyphal development occurred. Lower panels represent enlargements of the areas demarcated by rectangles for better visualization of the damage caused by the treatments to the fungi cell walls at the magnification of 255×; bars, 5 µm.

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques: Confocal Laser Scanning Microscopy, Staining, Inhibition

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Mechanism of cell death by necrosis ( A ) and apoptosis ( B ) induced after treatment of T. rubrum ATCC 28189 with nonyl, fluconazole, and amphotericin B. Both significantly induced necrotic death ( p < 0.05). * p < 0.05; ** p <0.01; *** p <0.001.

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques:

Journal: Pharmaceutics

Article Title: Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

doi: 10.3390/pharmaceutics15051402

Figure Lengend Snippet: Relative expression levels of Erg 1 and Erg 11 genes represented in Log2Fold change in T. rubrum ATCC 28189 strain treated with nonyl protocatechuate, compared to the untreated control. Results from three independent experiments are expressed as the mean ± standard deviation. Statistical significance was determined by the Student’s t -test and indicated by asterisks: ** p < 0.01.

Article Snippet: For the T. rubrum ATCC strain, treatment with terbinafine or nonyl significantly reduced the thickness of the biofilms on average to 33.23 µm ( B) and 39.28 µm ( C), respectively.

Techniques: Expressing, Control, Standard Deviation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Success of Topical Treatment of Onychomycosis Seems to Be Influenced by Fungal Features

doi: 10.1155/2021/5553634

Figure Lengend Snippet: Illustration of the invasiveness of Trichophyton rubrum on a healthy human nail in ten days. CMRP2912: isolated from patient 1; CMRP2918: isolated from patient 2. T. rubrum ATCC 40051. Topline: macroscopic aspects of fungal growth in nail fragments. Bottom line: features of fungal invasion as seen under a light microscope. Histological sections of nails stained by GMS, at 400 ×.

Article Snippet: Some laboratory tests were performed on the clinical isolates obtained from the two patients, T. rubrum CMRP2912 and T. rubrum CMRP2918, using the reference T. rubrum ATCC 40051 strain.

Techniques: Isolation, Light Microscopy, Staining