t phagedenis  (ATCC)


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    Name:
    Treponema phagedenis Kazan 8
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    Catalog Number:
    27087
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    Structured Review

    ATCC t phagedenis
    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. <t>phagedenis</t> , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    https://www.bioz.com/result/t phagedenis/product/ATCC
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    Images

    1) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿"

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00019-07

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Related Articles

    Sequencing:

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
    Article Snippet: .. Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ). .. Interestingly, considerable genetic diversity was detected among the isolates, not only from different cattle but also from the same individuals, using pulsed-field gel electrophoresis and PCR-based random amplified polymorphism DNA methods ( ).

    Isolation:

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
    Article Snippet: .. Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ). .. Interestingly, considerable genetic diversity was detected among the isolates, not only from different cattle but also from the same individuals, using pulsed-field gel electrophoresis and PCR-based random amplified polymorphism DNA methods ( ).

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
    Article Snippet: .. Glycolipid was extracted from the treponemes, including the T. phagedenis type strain ATCC 27087 and 8 T. phagedenis -like spirochetes isolated from different individual cows with PDD from different locations in Japan. ..

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
    Article Snippet: .. Coomassie brilliant blue staining showed that the banding patterns of whole-cell lysates were quite similar among all T. phagedenis -like spirochetes isolated from the PDD lesions examined, whereas the staining pattern of T. phagedenis type strain ATCC 27087 was partly different from that of the PDD isolates (Fig. ). .. Western blot analysis demonstrated that the treponemes examined had considerable antigenic diversity in view of their different banding patterns (Fig. ), although the SDS-PAGE profiles of their whole-cell lysates were similar (Fig. ).

    Staining:

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
    Article Snippet: .. Coomassie brilliant blue staining showed that the banding patterns of whole-cell lysates were quite similar among all T. phagedenis -like spirochetes isolated from the PDD lesions examined, whereas the staining pattern of T. phagedenis type strain ATCC 27087 was partly different from that of the PDD isolates (Fig. ). .. Western blot analysis demonstrated that the treponemes examined had considerable antigenic diversity in view of their different banding patterns (Fig. ), although the SDS-PAGE profiles of their whole-cell lysates were similar (Fig. ).

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  • 93
    ATCC t phagedenis atcc 27087
    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. <t>phagedenis</t> ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    T Phagedenis Atcc 27087, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t phagedenis atcc 27087/product/ATCC
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    t phagedenis atcc 27087 - by Bioz Stars, 2020-08
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    93
    ATCC t phagedenis
    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. <t>phagedenis</t> , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    T Phagedenis, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t phagedenis/product/ATCC
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    t phagedenis - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan ▿

    doi: 10.1128/JCM.01574-08

    Figure Lengend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Article Snippet: T. phagedenis ATCC 27087 and all PDD isolates were catalase positive and oxidase negative.

    Techniques:

    Comparisons of ELISA mean ODs for five individual cow sera against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes isolated from cows with PDD.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Comparisons of ELISA mean ODs for five individual cow sera against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes isolated from cows with PDD.

    Article Snippet: Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Isolation

    IgG antibody responses in sera of cows determined by ELISA, against T. phagedenis ATCC 27087 (a), T. phagedenis -like HT201 (b), T. phagedenis -like YG3903R (c), T. phagedenis -like HD26-43 (d), and T. phagedenis -like IZ6-2 (e). Group A, PDD-positive cows on PDD-positive farms ( n = 85); group B, PDD-negative cows on PDD-positive farms ( n = 33); group C, cows on PDD-free farms ( n = 33) for each ELISA. Each circle represents optical density at a wavelength of 492 nm. IgG levels against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes of groups A and B are significantly higher than that of group C, but there are no significant differences between groups A and B. S, significant ( P

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: IgG antibody responses in sera of cows determined by ELISA, against T. phagedenis ATCC 27087 (a), T. phagedenis -like HT201 (b), T. phagedenis -like YG3903R (c), T. phagedenis -like HD26-43 (d), and T. phagedenis -like IZ6-2 (e). Group A, PDD-positive cows on PDD-positive farms ( n = 85); group B, PDD-negative cows on PDD-positive farms ( n = 33); group C, cows on PDD-free farms ( n = 33) for each ELISA. Each circle represents optical density at a wavelength of 492 nm. IgG levels against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes of groups A and B are significantly higher than that of group C, but there are no significant differences between groups A and B. S, significant ( P

    Article Snippet: Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ).

    Techniques: Enzyme-linked Immunosorbent Assay

    SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Article Snippet: Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ).

    Techniques: SDS Page, Isolation

    Silver-stained profiles of glycolipid of Treponema phagedenis -like organisms isolated from cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass marker shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Silver-stained profiles of glycolipid of Treponema phagedenis -like organisms isolated from cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass marker shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Article Snippet: Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ).

    Techniques: Staining, Isolation, Marker

    Western blot reaction profiles of IgG antibody in sera from two individuals, cow YG3903 (A) and cow IZ6 (B), harboring PDD lesions, against glycolipid of Treponema phagedenis -like organisms isolated from different cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Western blot reaction profiles of IgG antibody in sera from two individuals, cow YG3903 (A) and cow IZ6 (B), harboring PDD lesions, against glycolipid of Treponema phagedenis -like organisms isolated from different cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Article Snippet: Recently, for the first time in Japan, we successfully isolated 40 spirochete isolates from dairy cattle with PDD lesions by using a simple two-step culture technique, and these isolates were identified as Treponema phagedenis -like spirochetes on the basis of their biochemical traits and enzyme activities, which were identical to those of T. phagedenis ATCC 27087, and the results of sequencing of the 16S rRNA gene showing that all isolates had > 99% identity to those of the T. phagedenis type strain and T. phagedenis -like spirochetes isolated from PDD lesions in the United States and Europe ( ).

    Techniques: Western Blot, Isolation

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Article Snippet: The data in this study suggest that they share antigenic determinants with T. phagedenis while, unlike T. phagedenis , they possess pathogenic potential.

    Techniques: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Article Snippet: The data in this study suggest that they share antigenic determinants with T. phagedenis while, unlike T. phagedenis , they possess pathogenic potential.

    Techniques: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Article Snippet: The data in this study suggest that they share antigenic determinants with T. phagedenis while, unlike T. phagedenis , they possess pathogenic potential.

    Techniques: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay