t phagedenis  (ATCC)


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    Structured Review

    ATCC t phagedenis
    SDS-PAGE profiles of whole-cell lysates of Treponema <t>phagedenis</t> -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.
    T Phagedenis, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis"

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis

    Journal:

    doi: 10.1128/CVI.00464-09

    SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.
    Figure Legend Snippet: SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Techniques Used: SDS Page, Isolation

    2) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    3) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    4) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    5) Product Images from "Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis "

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis

    Journal:

    doi: 10.1128/CVI.00464-09

    SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.
    Figure Legend Snippet: SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Techniques Used: SDS Page, Isolation

    6) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    7) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    8) Product Images from "Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan "

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01574-08

    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
    Figure Legend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Techniques Used: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    Figure Legend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Techniques Used:

    9) Product Images from "Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan "

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01574-08

    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
    Figure Legend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Techniques Used: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    Figure Legend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Techniques Used:

    10) Product Images from "Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan "

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01574-08

    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
    Figure Legend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Techniques Used: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    Figure Legend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Techniques Used:

    11) Product Images from "Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan "

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01574-08

    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
    Figure Legend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Techniques Used: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    Figure Legend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Techniques Used:

    12) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    13) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    14) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    15) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    16) Product Images from "Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan "

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.01574-08

    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
    Figure Legend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Techniques Used: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    Figure Legend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Techniques Used:

    17) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model "

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    Journal:

    doi: 10.1128/IAI.00019-07

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    18) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    19) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    20) Product Images from "Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species"

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species

    Journal: Veterinary Research

    doi: 10.1186/s13567-018-0605-z

    Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.
    Figure Legend Snippet: Species combinations within digital dermatitis lesions according to lesion grade. Each colour represents a mutually exclusive species composition (singlet, pair, triplet or quadruplet) within a lesion. Lesion-free, healing and chronic lesions depicted on the left and active lesions on the right. De: T. denticola ; Me: T. medium ; Pe: T. pedis ; Ph: T. phagedenis.

    Techniques Used:

    Related Articles

    Amplification:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: A fragment of the 16S rRNA gene (∼1,500 bp) of each 40 strains was amplified by using universal primers. .. The phylogenic tree (Fig. ) placed our PDD isolates (only strain YG3903R is shown in the figure) in a single cluster closely related to T. phagedenis ATCC 27087.

    Isolation:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: These phenotypic characteristics may change with passage number or culture conditions. .. In previous studies, the genetic diversity of T. phagedenis -like organisms isolated from PDD was assessed by molecular techniques such as PFGE and PCR-based restriction fragment length polymorphism , because epidemiological methods such as serotyping, biotyping, and outer membrane profiling have not been established for T. phagedenis -like spirochetes. .. Here, we used PFGE and RAPD to detect the heterogeneity of DNAs of T. phagedenis -like strains.

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: However, T. denticola and T. phagedenis isolated from humans were able to grow on the PDDTp medium used in the present study (data not shown). .. Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225.

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: BLAST searches of the sequence data showed 99 to 100% identity of all strains examined with each other, as well as with PDD strains isolated in the United States, the United Kingdom, and Denmark (accession numbers , , and , respectively). .. The phylogenic tree (Fig. ) placed our PDD isolates (only strain YG3903R is shown in the figure) in a single cluster closely related to T. phagedenis ATCC 27087.

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: T. phagedenis ATCC 27087 and T. denticola JCM8225 were used as type strains for comparing characteristics of treponemes from PDD lesions. .. T. phagedenis ATCC 27087 and T. denticola JCM8225 were used as type strains for comparing characteristics of treponemes from PDD lesions.

    Polymerase Chain Reaction:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: These phenotypic characteristics may change with passage number or culture conditions. .. In previous studies, the genetic diversity of T. phagedenis -like organisms isolated from PDD was assessed by molecular techniques such as PFGE and PCR-based restriction fragment length polymorphism , because epidemiological methods such as serotyping, biotyping, and outer membrane profiling have not been established for T. phagedenis -like spirochetes. .. Here, we used PFGE and RAPD to detect the heterogeneity of DNAs of T. phagedenis -like strains.

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: Thus, the existence of treponemes other than T. phagedenis -like spirochetes in PDD lesions of cattle in Japan should be determined by molecular biological methods, such as PCR and in situ hybridization. .. Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225.

    Incubation:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: The strains which did not recover one loopful of bacteria from one PDDTp agar plate after 14 days of incubation were not subjected to the tests. .. PDD isolates showed results similar to those of T. phagedenis ATCC 27087 but not to those of T. denticola (Table ).

    other:

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model
    Article Snippet: The data in this study suggest that they share antigenic determinants with T. phagedenis while, unlike T. phagedenis , they possess pathogenic potential.

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: Overall, the most common species compositions were T. phagedenis , T medium , and T. pedis (Ph/Me/Pe) and T. phagedenis , T medium , T. pedis , and T. denticola (Ph/Me/Pe/De) (21.1% and 20.4%, respectively) (Figure ).

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: The amount of T. denticola was weakly correlated with T. medium , T. phagedenis and T. pedis (R2 = 0.40, 0.32, and 0.30, respectively) indicating counts of T. medium , T. pedis and T. phagedenis have little effect on T. denticola counts and vice versa.

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: Despite T. phagedenis being the most common of the four Treponema species to be found in M0 samples, it was significantly (3.3–3.6 times) more likely that T. phagedenis would be present in M1 lesions, M2 lesions, and M4.1 lesions than M0 samples (P = 0.004, 0.0001, and 0.0001, respectively) (Table ).

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: Conversely, MBCs for T. phagedenis -like and T. medium /T. vincentii -like groups are 1.5–6 mg/L and 0.75 mg/L, respectively, supporting our culture viability findings following tetracycline treatment and suggesting T. denticola may be more resistant to treatment with oxytetracycline than T. medium , T. pedis and T. phagedenis [ ].

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: However, in M1 lesions T. medium , T. phagedenis , and T. pedis (Ph/Me/Pe) was the most common species composition (31.3% of M1 lesions), followed by all four species (Ph/Me/Pe/D) and T. medium and T. phagedenis together which were equally likely at 25% of M1 lesions each (Figure ).

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: Treponema counts between species indicated T. phagedenis and T. medium were the most correlated (R2 = 0.71), followed by T. medium and T. pedis (R2 = 0.49) and T. phagedenis and T. pedis (R2 = 0.49).

    Article Title: Associations between digital dermatitis lesion grades in dairy cattle and the quantities of four Treponema species
    Article Snippet: T. phagedenis and T. pedis are significantly more likely to be found in active lesions than healthy, healing, and chronic DD grades, supporting a relationship between host ulcerative pathology and both the presence and amount of T. pedis and T. phagedenis .

    Activity Assay:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: They showed weak beta-hemolytic activity on PDDTp agar plates supplemented with horse blood. .. PDD isolates showed results similar to those of T. phagedenis ATCC 27087 but not to those of T. denticola (Table ).

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: Our isolates were catalase positive and oxidase negative and showed weak beta-hemolytic activity. .. Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225.

    Sequencing:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225. .. Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225.

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: Paragraph title: 16S rRNA gene sequence analysis. ... The phylogenic tree (Fig. ) placed our PDD isolates (only strain YG3903R is shown in the figure) in a single cluster closely related to T. phagedenis ATCC 27087.

    In Situ Hybridization:

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan
    Article Snippet: Thus, the existence of treponemes other than T. phagedenis -like spirochetes in PDD lesions of cattle in Japan should be determined by molecular biological methods, such as PCR and in situ hybridization. .. Enzyme activities of all isolates examined were identical, and the enzyme profile was more closely related to that of T. phagedenis ATCC 27087 but not that of T. denticola JCM8225.

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    ATCC t phagedenis
    Transmission electron microscopy of T. <t>phagedenis</t> -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.
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    Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    doi: 10.1128/JCM.01574-08

    Figure Lengend Snippet: Transmission electron microscopy of T. phagedenis -like spirochete strain HT201 isolated from a PDD lesion. The spirochete has eight axial flagella at each end.

    Article Snippet: In previous studies, the genetic diversity of T. phagedenis -like organisms isolated from PDD was assessed by molecular techniques such as PFGE and PCR-based restriction fragment length polymorphism , because epidemiological methods such as serotyping, biotyping, and outer membrane profiling have not been established for T. phagedenis -like spirochetes.

    Techniques: Transmission Assay, Electron Microscopy, Isolation

    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan

    doi: 10.1128/JCM.01574-08

    Figure Lengend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Article Snippet: In previous studies, the genetic diversity of T. phagedenis -like organisms isolated from PDD was assessed by molecular techniques such as PFGE and PCR-based restriction fragment length polymorphism , because epidemiological methods such as serotyping, biotyping, and outer membrane profiling have not been established for T. phagedenis -like spirochetes.

    Techniques:

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Journal:

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 109 (closed square, solid line), 1010 (open square, dashed line), 1011 (closed circle, solid line), and 1011 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Article Snippet: The data in this study suggest that they share antigenic determinants with T. phagedenis while, unlike T. phagedenis , they possess pathogenic potential.

    Techniques: Mouse Assay