t denticola  (ATCC)


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    Structured Review

    ATCC t denticola
    Western blots with T. <t>denticola</t> Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    2) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿"

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00019-07

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    3) Product Images from "Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population"

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population

    Journal: BMC Infectious Diseases

    doi: 10.1186/s12879-016-1778-6

    Oral microbiota levels in the subgingival plaque from elderly individuals with periodontal health ( n = 17), gingivitis ( n = 19), and chronic periodontitis ( n = 15). The individual values represent bacterial counts per sample in the subgingival plaque ( a ) Total bacterial counts, ( b ) F. nucleatum , ( c ) P. intermedia , ( d ) P. gingivalis , ( e ) T. denticola , and ( f ) T. forsythia . The horizontal lines in the middle of the box represent the median and whiskers are drawn down to the 10 th through 90 th percentiles. The points below and above the whiskers are drawn as individual dots
    Figure Legend Snippet: Oral microbiota levels in the subgingival plaque from elderly individuals with periodontal health ( n = 17), gingivitis ( n = 19), and chronic periodontitis ( n = 15). The individual values represent bacterial counts per sample in the subgingival plaque ( a ) Total bacterial counts, ( b ) F. nucleatum , ( c ) P. intermedia , ( d ) P. gingivalis , ( e ) T. denticola , and ( f ) T. forsythia . The horizontal lines in the middle of the box represent the median and whiskers are drawn down to the 10 th through 90 th percentiles. The points below and above the whiskers are drawn as individual dots

    Techniques Used:

    4) Product Images from "Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization"

    Article Title: Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00320-13

    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Figure Legend Snippet: Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy

    Techniques Used:

    5) Product Images from "The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial"

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial

    Journal: PeerJ

    doi: 10.7717/peerj.4371

    The prevalence of four periodontal pathogens at different time points. SGAP group, supragingival glycine air polishing group; SUSP group, supragingival ultrasonic scaling group. (A) P. ginigvalis , (B) T. forsythia , (C) T. denticola , (D) F. nucleatum .
    Figure Legend Snippet: The prevalence of four periodontal pathogens at different time points. SGAP group, supragingival glycine air polishing group; SUSP group, supragingival ultrasonic scaling group. (A) P. ginigvalis , (B) T. forsythia , (C) T. denticola , (D) F. nucleatum .

    Techniques Used:

    Related Articles

    Diagnostic Assay:

    Article Title: Enzyme profiles of oral spirochetes in RapID-ANA system.
    Article Snippet: Enzyme profiles of oral Treponema species were determined by using RapID-ANA (Innovative Diagnostic System, Atlanta, Ga.), a 4-h test system which detects 18 enzymatic reactions, including aminopeptidases and glycosidases. .. Seventy-two clinical isolates of Treponema denticola, four reference strains of T. denticola (ATCC 35404, ATCC 35405, ATCC 35520, and ATCC 33521), one strain of T. vincentii (ATCC 35580), and two strains of T. socranskii subspecies (T. socranskii subsp. buccale ATCC 35534 and T. socranskii subsp. socranskii ATCC 35536) were used in this study.

    Amplification:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, DNA amplification and detection were performed in a qPCR system (StepOnePlus, Applied Biosystems Life Technologies, Basel, Switzerland), using master mix SYBR Green Master Mix (Applied Biosystems Life Technologies, Basel, Switzerland) for the amplification reaction. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Article Title: Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization
    Article Snippet: According to the probe match function in the Ribosomal Database Project (RDP) database , this primer set appeared to be specific for the Spirochaetaceae family, because it amplified mainly members of the genus Treponema . .. Of the 20 type strains that were present in the RDP database, this primer set targeted four members, i.e., T. putidum (ATCC 700334), T. denticola (ATCC 35405), T. medium (G7201), and T. pedis (T3552B).

    Positive Control:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    SYBR Green Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, DNA amplification and detection were performed in a qPCR system (StepOnePlus, Applied Biosystems Life Technologies, Basel, Switzerland), using master mix SYBR Green Master Mix (Applied Biosystems Life Technologies, Basel, Switzerland) for the amplification reaction. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Incubation:

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿
    Article Snippet: T. denticola (ATCC 35405) was grown in modified new oral spirochete broth (ATCC medium 1494). .. All of the bacteria were incubated under anaerobic conditions in a Coy anaerobic chamber (Coy Laboratory Products, Grass Lake, MI) under an atmosphere consisting of 85% N, 5% CO2 , and 10% H. All bacterial manipulations were carried out under anaerobic conditions.

    Activity Assay:

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. The endotoxin activity of the recombinant proteins was measured by the Limulus Amoebocyte Lysate (LAL) assay using a LAL Endochrome Kit (Charles River Endosafe, Wilmington, MA, USA) according to the manufacturer’s protocol.

    Cell Culture:

    Article Title: Multiplex real‐time PCR detection and relative quantification of periodontal pathogens
    Article Snippet: A. actinomycetemcomitans (ATCC 43718), F. nucleatum subsp. nucleatum (ATCC 25586), P. gingivalis (ATCC 33277), T. forsythia (ATCC 43037), and T. denticola (ATCC 33521) were obtained from American Type Culture Collection (Manassas, VA, USA). .. F. nucleatum and P. gingivalis were cultured in brain heart infusion, T. forsythia was cultured using brain heart infusion supplemented with 2.5 mM N‐acetylmuramic acid (Sigma, St. Louis, MO, USA).

    Article Title: Deficiency of Cathepsin K prevents inflammation and bone erosion in rheumatoid arthritis and periodontitis and reveals its shared osteoimmune role
    Article Snippet: The bacteria used in this study were Porphyromonas gingivalis W50 (ATCC: 53978), T. denticola (ATCC: 35404) and T. forsythia (ATCC: 43037). .. These strains were grown under anaerobic conditions (80% N2 , 10% H2 , and 10% CO2 ) at 37 °C in a Coy anaerobic chamber and were cultured [ , ].

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: .. T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. T. denticola surface protein Td92, the C-terminal half of Td92 (Td-B), the N-terminal half of Td92 (Td-G) and a partial peptide of Treponema lecithinolyticum MspTL(PP4) were prepared in recombinant six-histidine-tagged form by expression in E. coli and purification using Ni-NTA columns (Qiagen, Valencia, CA, USA) as described previously.

    Expressing:

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. T. denticola surface protein Td92, the C-terminal half of Td92 (Td-B), the N-terminal half of Td92 (Td-G) and a partial peptide of Treponema lecithinolyticum MspTL(PP4) were prepared in recombinant six-histidine-tagged form by expression in E. coli and purification using Ni-NTA columns (Qiagen, Valencia, CA, USA) as described previously.

    Modification:

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿
    Article Snippet: .. T. denticola (ATCC 35405) was grown in modified new oral spirochete broth (ATCC medium 1494). .. T. phagedenis (ATCC 27087) was grown in PY medium with cocarboxylase and serum (ATCC medium 1828).

    Electroporation:

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks
    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen. .. Nonmethylated plasmid DNA was prepared in E. coli SCS110 (Stratagene Corp., La Jolla, Calif.) for electroporation of T. denticola .

    Infection:

    Article Title: Deficiency of Cathepsin K prevents inflammation and bone erosion in rheumatoid arthritis and periodontitis and reveals its shared osteoimmune role
    Article Snippet: Paragraph title: Infection with bacterial strains ... The bacteria used in this study were Porphyromonas gingivalis W50 (ATCC: 53978), T. denticola (ATCC: 35404) and T. forsythia (ATCC: 43037).

    LAL Assay:

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. The endotoxin activity of the recombinant proteins was measured by the Limulus Amoebocyte Lysate (LAL) assay using a LAL Endochrome Kit (Charles River Endosafe, Wilmington, MA, USA) according to the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: .. The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Article Title: Porphyromonas gingivalis Fim-A genotype distribution among Colombians
    Article Snippet: .. PCR specificity for the P. gingivalis FimA gene Primers and PCR specificity of FimA genotypes (I, II, II, IV, and Ib) was probed against other periodontopathic bacteria like A. actinomycetemcomitans (D11-s1), T. denticola (ATCC. .. 43056), and T. forsythia (ATCC 43037).

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: .. Bacterial controls The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Recombinant:

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. T. denticola surface protein Td92, the C-terminal half of Td92 (Td-B), the N-terminal half of Td92 (Td-G) and a partial peptide of Treponema lecithinolyticum MspTL(PP4) were prepared in recombinant six-histidine-tagged form by expression in E. coli and purification using Ni-NTA columns (Qiagen, Valencia, CA, USA) as described previously.

    Crocin Bleaching Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, P. gingivalis (ATCC 33277 T; OMZ 925) was grown anaerobically on Columbia Blood Agar (CBA) agar plates for 3–4 days at 37 °C, followed by anaerobic subculturing for 2–3 days at 37 °C in broth. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    DNA Extraction:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: Paragraph title: DNA extraction and polymerase chain reaction measurements ... The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control.

    Nucleic Acid Electrophoresis:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Isolation:

    Article Title: Dioxygen and nitric oxide scavenging by Treponema denticola flavodiiron protein: a mechanistic paradigm for catalysis
    Article Snippet: T. denticola (ATCC 35405) was grown in new oral spirochete medium (NOS) with 10 % heat-inactivated rabbit serum, 10 mg of cocarboxylase per mL, at 36 °C in an anaerobic chamber (Coy Laboratory Products) containing an atmosphere of 85 % N2 /5 % CO2 /10 % H2 [ ]. .. Unless otherwise noted, the buffer for all reactions of the isolated Td FDP was 50 mM 3-( N -morpholino)-propanesulfonic acid (MOPS) pH 7.3, hereafter referred to as buffer.

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿
    Article Snippet: The PDD isolates were grown in prereduced, anaerobic oral treponeme isolation broth containing 10% inactivated fetal bovine serum without antibiotics that was prepared as previously described ( ). .. T. denticola (ATCC 35405) was grown in modified new oral spirochete broth (ATCC medium 1494).

    Purification:

    Article Title: Caspase-4 activation by a bacterial surface protein is mediated by cathepsin G in human gingival fibroblasts
    Article Snippet: T. denticola (ATCC 33521) was cultured in an anaerobic atmosphere (10% CO2 , 5% H2 and 85% N2 ) in new oral spirochete broth (ATCC medium 1494). .. T. denticola surface protein Td92, the C-terminal half of Td92 (Td-B), the N-terminal half of Td92 (Td-G) and a partial peptide of Treponema lecithinolyticum MspTL(PP4) were prepared in recombinant six-histidine-tagged form by expression in E. coli and purification using Ni-NTA columns (Qiagen, Valencia, CA, USA) as described previously.

    Plasmid Preparation:

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks
    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen. .. Nonmethylated plasmid DNA was prepared in E. coli SCS110 (Stratagene Corp., La Jolla, Calif.) for electroporation of T. denticola .

    Real-time Polymerase Chain Reaction:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Paragraph title: Bacterial quantification by qPCR ... T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Negative Control:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: .. The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. ..

    Article Title: Bacterial species associated with interdigital phlegmon outbreaks in Finnish dairy herds
    Article Snippet: Bacterial controls The following type strains were used as controls in the PCR assays: D. nodosus ATCC 25549, F. necrophorum ssp. necrophorum ATCC 25286, F. varium ATCC 8501, F. necrophorum ssp. funduliforme DSM 19678, T. pyogenes ATCC 19411D, P. levii (DSM23370) and P. melaninogenica (DSM26980), T. vincentii (ATCC 35580), T. phagedenis (ATCC 27087) and T. denticola (ATCC 3320). .. Our own Arcanobacterium haemolyticum isolate served as a negative control for T. pyogenes pyolysin.

    Agarose Gel Electrophoresis:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. The agarose gel was stained with GoldView type I (Solarbio, Beijing, China) and taken radiogram with 300 nm ultraviolet illumination.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. The agarose gel was stained with GoldView type I (Solarbio, Beijing, China) and taken radiogram with 300 nm ultraviolet illumination.

    Subculturing Assay:

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population
    Article Snippet: Briefly, P. gingivalis (ATCC 33277 T; OMZ 925) was grown anaerobically on Columbia Blood Agar (CBA) agar plates for 3–4 days at 37 °C, followed by anaerobic subculturing for 2–3 days at 37 °C in broth. .. T. forsythia (OMZ 1047), T. denticola (ATCC 35405; OMZ 661), P. intermedia (OMZ 278), F. nucleatum (OMZ 598), were maintained in liquid cultures as described earlier [ ].

    Produced:

    Article Title: Enzyme profiles of oral spirochetes in RapID-ANA system.
    Article Snippet: Seventy-two clinical isolates of Treponema denticola, four reference strains of T. denticola (ATCC 35404, ATCC 35405, ATCC 35520, and ATCC 33521), one strain of T. vincentii (ATCC 35580), and two strains of T. socranskii subspecies (T. socranskii subsp. buccale ATCC 35534 and T. socranskii subsp. socranskii ATCC 35536) were used in this study. .. All T. denticola strains produced indole and a variety of aminopeptidases and glycosidases.

    Marker:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. Polymerase chain reaction amplification was carried out with GeneAmp PCR system 2700 (ABI, South San Francisco, CA, USA), and the parameters were as follows: PCR mixtures contained 2 μl template, 1 μl primer (10 μM) primer, 2.5 μl 10× buffer Mg2+ plus, 2 μl dNTP (2.5 mM), 0.2 μl Taq DNA polymerase (5 U/μl) (TaKaRa Biotechnology, Dalian, China), 17.3 μl ddH2 O. Thermocycling for P. gingivalis , T. forsythia , T. denticola was 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Thermocycling for F. nucleatum was 94 °C 1 min, 60 °C 1 min, 72 °C 90 s, 35 cycles; 72 °C 10 min, 95 °C 2 min, 95 °C 30 s, 60 °C 1 min, 72 °C 1 min, 36 cycles; 72 °C 2 min. Polymerase chain reaction products were analyzed by Horizontal Gel Electrophoresis with 1.5% agarose (Solarbio, Beijing, China), 130 V. DL 2000 ladder (TaKaRa Biotechnology, Dalian, China) served as the DNA weight marker.

    Staining:

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. The agarose gel was stained with GoldView type I (Solarbio, Beijing, China) and taken radiogram with 300 nm ultraviolet illumination.

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial
    Article Snippet: The DNA extracted from P. gingivalis (ATCC 51700), T. forsythia (ATCC 43037), T. denticola (ATCC 33520) and F. nucleatum (ATCC 25586) were used as positive control for PCR analysis, and ultrapure water was used as negative control. .. The agarose gel was stained with GoldView type I (Solarbio, Beijing, China) and taken radiogram with 300 nm ultraviolet illumination.

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    ATCC t denticola
    Hypothetical model for stepwise degradation of glutathione in T. <t>denticola</t> . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques: Cell Culture