t denticola  (ATCC)


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    Structured Review

    ATCC t denticola
    Hypothetical model for stepwise degradation of glutathione in T. <t>denticola</t> . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    2) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    3) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    4) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    5) Product Images from "Treponema denticola Outer Membrane Enhances the Phagocytosis of Collagen-Coated Beads by Gingival Fibroblasts"

    Article Title: Treponema denticola Outer Membrane Enhances the Phagocytosis of Collagen-Coated Beads by Gingival Fibroblasts

    Journal: Infection and Immunity

    doi:

    Cytochalasin D treatment of HGFs abolished the T. denticola OM-induced enhancement of bead uptake. HGFs were counted and exposed to CIM control (Cont) or T. denticola OM (0.192 ng of OM/HGF for 1 h). Cells were washed free of the extract, recounted, and then incubated in either α-MEM or 1 μM cytochalasin D (Cyt D) for 1 h. PI is expressed as mean ± SE (three independent samples of 10,000 cells each). ∗, P
    Figure Legend Snippet: Cytochalasin D treatment of HGFs abolished the T. denticola OM-induced enhancement of bead uptake. HGFs were counted and exposed to CIM control (Cont) or T. denticola OM (0.192 ng of OM/HGF for 1 h). Cells were washed free of the extract, recounted, and then incubated in either α-MEM or 1 μM cytochalasin D (Cyt D) for 1 h. PI is expressed as mean ± SE (three independent samples of 10,000 cells each). ∗, P

    Techniques Used: Incubation

    Effect of V. atypica OM challenge on HGF phagocytosis phenotype. (A) HGFs were exposed to CIM (negative control), T. denticola OM (positive control), or V. atypica OM (0.192 ng [dry weight] of OM/HGF cell for 1 h). In contrast to T. denticola , V. atypica OM pretreatment induced a decrease in collagen (Col)-coated bead phagocytosis and no statistically significant change in BSA-coated bead uptake. For each condition, three independent samples of 10,000 cells each were assayed (mean ± SE). Data shown are expressed as percent difference from negative control HGFs. ∗, P = 0.001; ∗∗, P
    Figure Legend Snippet: Effect of V. atypica OM challenge on HGF phagocytosis phenotype. (A) HGFs were exposed to CIM (negative control), T. denticola OM (positive control), or V. atypica OM (0.192 ng [dry weight] of OM/HGF cell for 1 h). In contrast to T. denticola , V. atypica OM pretreatment induced a decrease in collagen (Col)-coated bead phagocytosis and no statistically significant change in BSA-coated bead uptake. For each condition, three independent samples of 10,000 cells each were assayed (mean ± SE). Data shown are expressed as percent difference from negative control HGFs. ∗, P = 0.001; ∗∗, P

    Techniques Used: Negative Control, Positive Control

    Concentration-related effect of T. denticola OM challenge on HGF-specific (Co-I) and nonspecific (BSA) bead uptake. HGFs were treated with OM at final concentrations of 0.096, 0.192, and 0.288 ng (dry weight) of OM/HGF cell for 1 h. PI is expressed as mean ± SE (three independent samples of 10,000 cells each; note that error bars are very small). ∗, P
    Figure Legend Snippet: Concentration-related effect of T. denticola OM challenge on HGF-specific (Co-I) and nonspecific (BSA) bead uptake. HGFs were treated with OM at final concentrations of 0.096, 0.192, and 0.288 ng (dry weight) of OM/HGF cell for 1 h. PI is expressed as mean ± SE (three independent samples of 10,000 cells each; note that error bars are very small). ∗, P

    Techniques Used: Concentration Assay

    Effect of duration of T. denticola OM challenge on HGF-specific (Co-I) and nonspecific (BSA) bead uptake. HGFs were treated with T. denticola OM (0.192 ng [dry weight] of OM/HGF cell) for 15, 30, or 60 min. PI is expressed as mean ± SE (three independent samples of 10,000 cells each; note that error bars are very small). ∗, P
    Figure Legend Snippet: Effect of duration of T. denticola OM challenge on HGF-specific (Co-I) and nonspecific (BSA) bead uptake. HGFs were treated with T. denticola OM (0.192 ng [dry weight] of OM/HGF cell) for 15, 30, or 60 min. PI is expressed as mean ± SE (three independent samples of 10,000 cells each; note that error bars are very small). ∗, P

    Techniques Used:

    T. denticola OM-induced phagocytosis enhancement is a long-lasting effect. Group 1, phagocytosis assay tested immediately following washing of the HGFs free of the OM; group 2, treatment of HGFs with OM followed by washing and overnight incubation with serum-free medium. The phagocytosis enhancement persisted. PI is expressed as mean ± SE (three independent samples of 10,000 cells each). ∗, P
    Figure Legend Snippet: T. denticola OM-induced phagocytosis enhancement is a long-lasting effect. Group 1, phagocytosis assay tested immediately following washing of the HGFs free of the OM; group 2, treatment of HGFs with OM followed by washing and overnight incubation with serum-free medium. The phagocytosis enhancement persisted. PI is expressed as mean ± SE (three independent samples of 10,000 cells each). ∗, P

    Techniques Used: Phagocytosis Assay, Incubation

    Cell cycle analysis following T. denticola OM challenge. Flow cytometry indicated no change in the sub-G 1 population. Cells were exposed to OM-free CO 2 -independent medium (A) or T. denticola OM (0.192 ng/cell) for 15 min (B), 30 min (C), or 60 min (D). Cells were washed free of the extract and assayed for cell cycle phases. Prior to flow cytometry analysis, cells were fixed with 70% ethanol at 4°C for 5 min and then centrifuged and resuspended in sulforadamine with DAPI. The flow cytometer was configured to analyze only cells with the forward scatter and side scatter characteristics of whole cells. M1 indicates the sub-G 1 population.
    Figure Legend Snippet: Cell cycle analysis following T. denticola OM challenge. Flow cytometry indicated no change in the sub-G 1 population. Cells were exposed to OM-free CO 2 -independent medium (A) or T. denticola OM (0.192 ng/cell) for 15 min (B), 30 min (C), or 60 min (D). Cells were washed free of the extract and assayed for cell cycle phases. Prior to flow cytometry analysis, cells were fixed with 70% ethanol at 4°C for 5 min and then centrifuged and resuspended in sulforadamine with DAPI. The flow cytometer was configured to analyze only cells with the forward scatter and side scatter characteristics of whole cells. M1 indicates the sub-G 1 population.

    Techniques Used: Cell Cycle Assay, Flow Cytometry, Cytometry

    Effect of T. denticola OM challenge on HGF pinocytic activity. (A) HGFs were incubated with LY at a final concentration of 0.5 mg/ml and T. denticola OM (0.192 ng/HGF; dashed line), or without the OM extract (solid line), for 1, 10, or 15 min. P
    Figure Legend Snippet: Effect of T. denticola OM challenge on HGF pinocytic activity. (A) HGFs were incubated with LY at a final concentration of 0.5 mg/ml and T. denticola OM (0.192 ng/HGF; dashed line), or without the OM extract (solid line), for 1, 10, or 15 min. P

    Techniques Used: Activity Assay, Incubation, Concentration Assay

    6) Product Images from "The Chymotrypsin-Like Protease Complex of Treponema denticola ATCC 35405 Mediates Fibrinogen Adherence and Degradation ▿"

    Article Title: The Chymotrypsin-Like Protease Complex of Treponema denticola ATCC 35405 Mediates Fibrinogen Adherence and Degradation ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00258-07

    TCT in the absence or presence of T. denticola ATCC 35405, MHE (Msp − ), or CKE (CTLP − ). Human plasma was incubated with T. denticola (1.2 × 10 9 cells/ml) suspended in PBS or with PBS alone (control) for 5 min before the addition
    Figure Legend Snippet: TCT in the absence or presence of T. denticola ATCC 35405, MHE (Msp − ), or CKE (CTLP − ). Human plasma was incubated with T. denticola (1.2 × 10 9 cells/ml) suspended in PBS or with PBS alone (control) for 5 min before the addition

    Techniques Used: Incubation

    Fibrinogen zymograms of outer membrane proteins from T. denticola ATCC 35405, MHE (Msp − ), and CKE (CTLP − ). Triton X-114 extracts were incubated for 5 min at 20°C (−) or 100°C (+) before being subjected to
    Figure Legend Snippet: Fibrinogen zymograms of outer membrane proteins from T. denticola ATCC 35405, MHE (Msp − ), and CKE (CTLP − ). Triton X-114 extracts were incubated for 5 min at 20°C (−) or 100°C (+) before being subjected to

    Techniques Used: Incubation

    Purification and enzymatic activity of major fibrinogen-binding protein from T. denticola ATCC 35405. Outer membrane proteins were extracted with 0.1% Triton X-114 solution and incubated with fibrinogen-linked Sepharose, and then tightly bound
    Figure Legend Snippet: Purification and enzymatic activity of major fibrinogen-binding protein from T. denticola ATCC 35405. Outer membrane proteins were extracted with 0.1% Triton X-114 solution and incubated with fibrinogen-linked Sepharose, and then tightly bound

    Techniques Used: Purification, Activity Assay, Binding Assay, Incubation

    Binding of fluid-phase or immobilized fibrinogen by T. denticola ATCC 35405 (⧫), MHE (Msp − ; ▴), or CKE (CTLP − ; ▪). (A and B) Treponema cells immobilized onto plastic wells in the absence (A) or presence (B) of 0.05
    Figure Legend Snippet: Binding of fluid-phase or immobilized fibrinogen by T. denticola ATCC 35405 (⧫), MHE (Msp − ; ▴), or CKE (CTLP − ; ▪). (A and B) Treponema cells immobilized onto plastic wells in the absence (A) or presence (B) of 0.05

    Techniques Used: Binding Assay

    Fibrinogen blot overlay of outer membrane protein extracts from T. denticola ATCC 35405, MHE, and CKE. Proteins were extracted with 1% Triton X-114 and incubated for 5 min at 20°C (−) or 100°C (+) before being subjected
    Figure Legend Snippet: Fibrinogen blot overlay of outer membrane protein extracts from T. denticola ATCC 35405, MHE, and CKE. Proteins were extracted with 1% Triton X-114 and incubated for 5 min at 20°C (−) or 100°C (+) before being subjected

    Techniques Used: Incubation

    Adherence of T. denticola ATCC 35405 cells to denatured fibrinogen or recombinant fibrinogen polypeptides. (A) Human fibrinogen (N) or purified recombinant Aα, Bβ, and γ chain polypeptides were heated for 5 min at 100°C
    Figure Legend Snippet: Adherence of T. denticola ATCC 35405 cells to denatured fibrinogen or recombinant fibrinogen polypeptides. (A) Human fibrinogen (N) or purified recombinant Aα, Bβ, and γ chain polypeptides were heated for 5 min at 100°C

    Techniques Used: Recombinant, Purification

    7) Product Images from "Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities"

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    Journal: Microbiology

    doi: 10.1099/mic.0.055939-0

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were
    Figure Legend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Techniques Used: Confocal Laser Scanning Microscopy

    Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments
    Figure Legend Snippet: Activity and electrophoretic profiles of CTLP. (a) SAAPFNA hydrolysis by T. denticola and T. vincentii cell suspensions was detected spectrophotometrically at 405 nm, as described in Methods. Error bars, sd of the mean calculated from triplicate experiments

    Techniques Used: Activity Assay

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580
    Figure Legend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Techniques Used:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72
    Figure Legend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Techniques Used: Fluorescence, Microscopy

    Effect of the protease inhibitor PMSF on adhesion. Biotinylated T. denticola cells were incubated with 0.1 mM PMSF for 30 min at 37 °C (□), or left untreated (•), and then assayed for adhesion to glutaraldehyde-fixed P. gingivalis
    Figure Legend Snippet: Effect of the protease inhibitor PMSF on adhesion. Biotinylated T. denticola cells were incubated with 0.1 mM PMSF for 30 min at 37 °C (□), or left untreated (•), and then assayed for adhesion to glutaraldehyde-fixed P. gingivalis

    Techniques Used: Protease Inhibitor, Incubation

    Representative CLSM images of T. denticola and P. gingivalis dual-species biofilms formed on saliva-coated coverslips (72 h). The images show that P. gingivalis cells are mainly localized attached to the substratum [(a); depth, 2 µm], whereas
    Figure Legend Snippet: Representative CLSM images of T. denticola and P. gingivalis dual-species biofilms formed on saliva-coated coverslips (72 h). The images show that P. gingivalis cells are mainly localized attached to the substratum [(a); depth, 2 µm], whereas

    Techniques Used: Confocal Laser Scanning Microscopy

    8) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    9) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    10) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    11) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    12) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    13) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    14) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    15) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

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    Clone Assay:

    Article Title:
    Article Snippet: .. Orientation of the tap1 gene and ermF-ermAM cassette was determined by PCR and DNA sequencing; only those clones containing the ermF-ermAM cassette oriented in the same direction as tap1 were chosen for the insertional inactivation in T. denticola . ..

    Amplification:

    Article Title:
    Article Snippet: .. To obtain a partial sequence from one of these genes, primers from conserved regions of T. phagedenis flaB were used in a PCR amplification of T. denticola ( ). .. The primers FLAB2 (5′-GTGGTTCCATATCGGGGCC-3′) and FLAB4 (5′-CCTGCAAAAAGTTTAGCGC-3′) amplified a 620-bp fragment of T. denticola DNA which was cloned into the pCRII-TOPO cloning vector and sequenced to confirm a flaB identity.

    Electroporation:

    Article Title:
    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen. .. Nonmethylated plasmid DNA was prepared in E. coli SCS110 (Stratagene Corp., La Jolla, Calif.) for electroporation of T. denticola .

    Mutagenesis:

    Article Title:
    Article Snippet: .. The transformation of T. denticola with the ermF-ermAM np cassette produced another mutant, termed AS98. ..

    Article Title:
    Article Snippet: Addition of T. denticola ATCC 35405 cells led to an increased TCT, whereas mutant CKE or T. vincentii ATCC 35580 cells did not affect the time taken to induce clot formation ( ). .. To rule out that the effects resulted simply from greater numbers of bacteria being present in the dual-species experiments, controls were included with twice the numbers of T. denticola or P. gingivalis ATCC 33277 cells, but the TCTs for the individual species were not increased.

    Sequencing:

    Article Title:
    Article Snippet: .. The probe was a segment of flgE made by PCR of T. denticola with a DNA sequence available from Li et al. ( ) (GenBank accession no. ). .. DNA sequencing at the Wadsworth Center Molecular Genetics Core facility was done with the Perkin-Elmer ABI Prism 377 and ABI 373A sequencers.

    Article Title:
    Article Snippet: .. To obtain a partial sequence from one of these genes, primers from conserved regions of T. phagedenis flaB were used in a PCR amplification of T. denticola ( ). .. The primers FLAB2 (5′-GTGGTTCCATATCGGGGCC-3′) and FLAB4 (5′-CCTGCAAAAAGTTTAGCGC-3′) amplified a 620-bp fragment of T. denticola DNA which was cloned into the pCRII-TOPO cloning vector and sequenced to confirm a flaB identity.

    Cell Culture:

    Article Title:
    Article Snippet: .. As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities. ..

    Produced:

    Article Title:
    Article Snippet: .. The transformation of T. denticola with the ermF-ermAM np cassette produced another mutant, termed AS98. ..

    Concentration Assay:

    Article Title:
    Article Snippet: To test whether the HGF phagocytosis phenotype can be enhanced by an OM extract of a bacterium other than T. denticola , the OM extract of V. atypica ATCC 17744 was used. .. In fact, V. atypica OM concentrations up to four times the standard concentration (0.192 ng/cell) yielded a concentration-dependent decrease in HGF uptake of collagen-coated beads (Fig. B).

    Incubation:

    Article Title:
    Article Snippet: When treponemes were incubated with fresh human plasma, in combination with equal numbers of P. gingivalis ATCC 33277 cells, TCTs were found to be significantly ( P < 0.01) greater than those observed with each individual species , and were greatest only when CTLP was expressed. .. To rule out that the effects resulted simply from greater numbers of bacteria being present in the dual-species experiments, controls were included with twice the numbers of T. denticola or P. gingivalis ATCC 33277 cells, but the TCTs for the individual species were not increased.

    other:

    Article Title:
    Article Snippet: The protocol developed by Li et al. was used with some minor modifications for insertional inactivation of T. denticola ( ).

    Article Title:
    Article Snippet: Our hypothesis is that Tap1 is involved in monitoring the length of the flagellar hook in T. denticola , a function that is performed by FliK in enteric bacteria ( , , , ).

    Article Title:
    Article Snippet: Approximately 109 cells of T. denticola were washed twice with Tris-buffered saline, the outer sheath was removed with 2% Triton X-100, and the cells were washed once with Tris-buffered saline.

    Article Title:
    Article Snippet: Although previous work has demonstrated the adherence of T. denticola to immobilized fibrinogen , the present study provides evidence that T. denticola ATCC 35405 binds both fluid-phase and immobilized fibrinogen and provides a molecular basis for these interactions.

    DNA Sequencing:

    Article Title:
    Article Snippet: .. Orientation of the tap1 gene and ermF-ermAM cassette was determined by PCR and DNA sequencing; only those clones containing the ermF-ermAM cassette oriented in the same direction as tap1 were chosen for the insertional inactivation in T. denticola . ..

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. The probe was a segment of flgE made by PCR of T. denticola with a DNA sequence available from Li et al. ( ) (GenBank accession no. ). .. DNA sequencing at the Wadsworth Center Molecular Genetics Core facility was done with the Perkin-Elmer ABI Prism 377 and ABI 373A sequencers.

    Article Title:
    Article Snippet: .. To obtain a partial sequence from one of these genes, primers from conserved regions of T. phagedenis flaB were used in a PCR amplification of T. denticola ( ). .. The primers FLAB2 (5′-GTGGTTCCATATCGGGGCC-3′) and FLAB4 (5′-CCTGCAAAAAGTTTAGCGC-3′) amplified a 620-bp fragment of T. denticola DNA which was cloned into the pCRII-TOPO cloning vector and sequenced to confirm a flaB identity.

    Article Title:
    Article Snippet: .. Orientation of the tap1 gene and ermF-ermAM cassette was determined by PCR and DNA sequencing; only those clones containing the ermF-ermAM cassette oriented in the same direction as tap1 were chosen for the insertional inactivation in T. denticola . ..

    Coagulation:

    Article Title:
    Article Snippet: It has been hypothesized that CTLP plays an important role in interference with the blood coagulation cascade ( ). .. To rule out that the effects resulted simply from greater numbers of bacteria being present in the dual-species experiments, controls were included with twice the numbers of T. denticola or P. gingivalis ATCC 33277 cells, but the TCTs for the individual species were not increased.

    Transformation Assay:

    Article Title:
    Article Snippet: .. The transformation of T. denticola with the ermF-ermAM np cassette produced another mutant, termed AS98. ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: T. denticola (ATCC 33520) was grown in new oral spirochete medium (NOS) with 10% heat-inactivated rabbit serum and 10 μg of cocarboxylase per ml at 36°C in an anaerobic chamber (Coy Laboratory Products, Inc., Grass Lake, Mich.) with an atmosphere of 85% nitrogen, 5% carbon dioxide, and 10% hydrogen. .. Nonmethylated plasmid DNA was prepared in E. coli SCS110 (Stratagene Corp., La Jolla, Calif.) for electroporation of T. denticola .

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    ATCC t denticola
    Hypothetical model for stepwise degradation of glutathione in T. <t>denticola</t> . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    T Denticola, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Journal: Infection and Immunity

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Figure Lengend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Article Snippet: As shown in Table , T. denticola cultured with glutathione (reduced form) or Cys-Gly exhibited approximately 20- and 10-fold greater hemoxidative and hemolytic activities, respectively, than T. denticola cultured with other compounds which are not substrates of T. denticola that yield H2 S. As a control, oxidized glutathione, which is not a suitable metabolic substrate for T. denticola , had no hemoxidative and hemolytic activities.

    Techniques: Cell Culture