t denticola  (ATCC)


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  • 94
    Name:
    Treponema denticola a CIP 103919 DSM 14222
    Description:

    Catalog Number:
    35405
    Price:
    None
    Applications:
    Produces major surface proteinProduces methyl-accepting chemotaxis proteinProduces prolyl aminopeptidase proline iminopeptidase
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    Structured Review

    ATCC t denticola
    Oral microbiota levels in the subgingival plaque from elderly individuals with periodontal health ( n = 17), gingivitis ( n = 19), and chronic periodontitis ( n = 15). The individual values represent bacterial counts per sample in the subgingival plaque ( a ) Total bacterial counts, ( b ) F. nucleatum , ( c ) P. intermedia , ( d ) P. gingivalis , ( e ) T. <t>denticola</t> , and ( f ) T. forsythia . The horizontal lines in the middle of the box represent the median and whiskers are drawn down to the 10 th through 90 th percentiles. The points below and above the whiskers are drawn as individual dots

    https://www.bioz.com/result/t denticola/product/ATCC
    Average 94 stars, based on 11 article reviews
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    t denticola - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population"

    Article Title: Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population

    Journal: BMC Infectious Diseases

    doi: 10.1186/s12879-016-1778-6

    Oral microbiota levels in the subgingival plaque from elderly individuals with periodontal health ( n = 17), gingivitis ( n = 19), and chronic periodontitis ( n = 15). The individual values represent bacterial counts per sample in the subgingival plaque ( a ) Total bacterial counts, ( b ) F. nucleatum , ( c ) P. intermedia , ( d ) P. gingivalis , ( e ) T. denticola , and ( f ) T. forsythia . The horizontal lines in the middle of the box represent the median and whiskers are drawn down to the 10 th through 90 th percentiles. The points below and above the whiskers are drawn as individual dots
    Figure Legend Snippet: Oral microbiota levels in the subgingival plaque from elderly individuals with periodontal health ( n = 17), gingivitis ( n = 19), and chronic periodontitis ( n = 15). The individual values represent bacterial counts per sample in the subgingival plaque ( a ) Total bacterial counts, ( b ) F. nucleatum , ( c ) P. intermedia , ( d ) P. gingivalis , ( e ) T. denticola , and ( f ) T. forsythia . The horizontal lines in the middle of the box represent the median and whiskers are drawn down to the 10 th through 90 th percentiles. The points below and above the whiskers are drawn as individual dots

    Techniques Used:

    2) Product Images from "Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks"

    Article Title: Insertional Inactivation of Treponemadenticola tap1 Results in a Nonmotile Mutant with Elongated Flagellar Hooks

    Journal: Journal of Bacteriology

    doi:

    Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.
    Figure Legend Snippet: Western blots with T. denticola Tap1 antiserum (A), T. pallidum FlgE antiserum (B), and T. phagedenis FlaB antiserum (C). Lanes 1, JS97; lanes 2, AS98; lanes 3, wild type. The arrow in panel A indicates the Tap1 polypeptide band. In panel B, the polypeptide ladder is a typical pattern found in treponemal hook polypeptides that may be due to cross-linking. The significance of the minor band missing in lane 3 is unknown. Numbers at right of each panel represent molecular masses in kilodaltons.

    Techniques Used: Western Blot

    Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.
    Figure Legend Snippet: Diagram of the fla operon organization of T. denticola and 5′ upstream region. P fla indicates the approximate location of the fla operon promoter. The ermF-ermAM cassette is indicated above the operon and is shown where it is inserted in the Bgl II site for creating Tap1-deficient mutants. The locations of primer pairs used for RT-PCR are indicated by arrows and are represented as follows: 1, TDW8; 2, TDW9; 3, TDW12; 4, TDW5; 5, TDWFLGEF; 6, TDWFLGER. Sequences of the primers are given in Materials and Methods.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.
    Figure Legend Snippet: Comparison of the T. denticola fla promoter sequence with promoter sequences from various spirochete motility genes and consensus sigma 28 sequences.

    Techniques Used: Sequencing

    Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.
    Figure Legend Snippet: Alignment of Tap1 amino acid sequences. (A) Identical amino acids from three treponemes are boxed and shaded with SHADYBOX, which reveals the conserved region near the carboxyl terminus. The dark inverted triangle indicates the location of the point of insertion for the erythromycin resistance cassette into the T. denticola tap1 gene to generate a Tap1-deficient mutant. (B) Alignment of the conserved C-terminal region of T. denticola Tap1 with FliK of S. typhimurium ). Identical amino acids are boxed and shaded as described above.

    Techniques Used: Mutagenesis

    T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.
    Figure Legend Snippet: T. denticola wild type (WT) and Tap1-deficient mutant JS97 after growth for 7 days on NOS plates containing 0.5% agarose. Approximately 0.1 μl was placed on the plate and incubated at 36°C in an anaerobic chamber.

    Techniques Used: Mutagenesis, Incubation

    3) Product Images from "Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization"

    Article Title: Targeting the Treponemal Microbiome of Digital Dermatitis Infections by High-Resolution Phylogenetic Analyses and Comparison with Fluorescent In Situ Hybridization

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.00320-13

    Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy
    Figure Legend Snippet: Relative frequencies of occurrence of the 4 most prevalent treponemal clusters ( T. denticola / T. pedis -like, T. phagedenis -like, T. medium / T. vincentii -like, and T. refringens -like) among the 36 digital dermatitis biopsy specimens (a) and in the 36 biopsy

    Techniques Used:

    4) Product Images from "Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum"

    Article Title: Conservation of the Host-Interacting Proteins Tp0750 and Pallilysin among Treponemes and Restriction of Proteolytic Capacity to Treponema pallidum

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00643-15

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, bind host proteins. Binding assays were performed to compare attachment of 2 μg of each of recombinant Tp0750, pallilysin, Tde0841, DD750, Tde0840, and DD751

    Techniques Used: Binding Assay, Recombinant

    Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three
    Figure Legend Snippet: Tp0750 and pallilysin, but not orthologs from T. denticola and T. phagedenis strain 4A, degrade host proteins. (A) In vitro SDS-PAGE-based assays were performed to compare the host component-degrading capabilities of Tp0750, Tde0841, and DD750. The three

    Techniques Used: In Vitro, SDS Page

    5) Product Images from "The Chymotrypsin-Like Protease Complex of Treponema denticola ATCC 35405 Mediates Fibrinogen Adherence and Degradation ▿"

    Article Title: The Chymotrypsin-Like Protease Complex of Treponema denticola ATCC 35405 Mediates Fibrinogen Adherence and Degradation ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00258-07

    TCT in the absence or presence of T. denticola ATCC 35405, MHE (Msp − ), or CKE (CTLP − ). Human plasma was incubated with T. denticola (1.2 × 10 9 cells/ml) suspended in PBS or with PBS alone (control) for 5 min before the addition
    Figure Legend Snippet: TCT in the absence or presence of T. denticola ATCC 35405, MHE (Msp − ), or CKE (CTLP − ). Human plasma was incubated with T. denticola (1.2 × 10 9 cells/ml) suspended in PBS or with PBS alone (control) for 5 min before the addition

    Techniques Used: Incubation

    Fibrinogen zymograms of outer membrane proteins from T. denticola ATCC 35405, MHE (Msp − ), and CKE (CTLP − ). Triton X-114 extracts were incubated for 5 min at 20°C (−) or 100°C (+) before being subjected to
    Figure Legend Snippet: Fibrinogen zymograms of outer membrane proteins from T. denticola ATCC 35405, MHE (Msp − ), and CKE (CTLP − ). Triton X-114 extracts were incubated for 5 min at 20°C (−) or 100°C (+) before being subjected to

    Techniques Used: Incubation

    Purification and enzymatic activity of major fibrinogen-binding protein from T. denticola ATCC 35405. Outer membrane proteins were extracted with 0.1% Triton X-114 solution and incubated with fibrinogen-linked Sepharose, and then tightly bound
    Figure Legend Snippet: Purification and enzymatic activity of major fibrinogen-binding protein from T. denticola ATCC 35405. Outer membrane proteins were extracted with 0.1% Triton X-114 solution and incubated with fibrinogen-linked Sepharose, and then tightly bound

    Techniques Used: Purification, Activity Assay, Binding Assay, Incubation

    Binding of fluid-phase or immobilized fibrinogen by T. denticola ATCC 35405 (⧫), MHE (Msp − ; ▴), or CKE (CTLP − ; ▪). (A and B) Treponema cells immobilized onto plastic wells in the absence (A) or presence (B) of 0.05
    Figure Legend Snippet: Binding of fluid-phase or immobilized fibrinogen by T. denticola ATCC 35405 (⧫), MHE (Msp − ; ▴), or CKE (CTLP − ; ▪). (A and B) Treponema cells immobilized onto plastic wells in the absence (A) or presence (B) of 0.05

    Techniques Used: Binding Assay

    Fibrinogen blot overlay of outer membrane protein extracts from T. denticola ATCC 35405, MHE, and CKE. Proteins were extracted with 1% Triton X-114 and incubated for 5 min at 20°C (−) or 100°C (+) before being subjected
    Figure Legend Snippet: Fibrinogen blot overlay of outer membrane protein extracts from T. denticola ATCC 35405, MHE, and CKE. Proteins were extracted with 1% Triton X-114 and incubated for 5 min at 20°C (−) or 100°C (+) before being subjected

    Techniques Used: Incubation

    Adherence of T. denticola ATCC 35405 cells to denatured fibrinogen or recombinant fibrinogen polypeptides. (A) Human fibrinogen (N) or purified recombinant Aα, Bβ, and γ chain polypeptides were heated for 5 min at 100°C
    Figure Legend Snippet: Adherence of T. denticola ATCC 35405 cells to denatured fibrinogen or recombinant fibrinogen polypeptides. (A) Human fibrinogen (N) or purified recombinant Aα, Bβ, and γ chain polypeptides were heated for 5 min at 100°C

    Techniques Used: Recombinant, Purification

    6) Product Images from "Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression"

    Article Title: Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

    Journal: Infection and Immunity

    doi: 10.1128/IAI.70.3.1113-1120.2002

    Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.
    Figure Legend Snippet: Hypothetical model for stepwise degradation of glutathione in T. denticola . γGTase, γ-glutamyltransferase; CGase, cysteinyl glycinase.

    Techniques Used:

    Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P
    Figure Legend Snippet: Effects of thiol compounds on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium or basic medium containing various chemicals at a concentration of 6 mM. After 2 days, the OD 660 was determined to monitor bacterial growth. GSH, glutathione. The bars and error bars indicate means and standard deviations, respectively ( n = 3). The values for cultures grown in the presence of glutathione, Cys-Gly, cysteine, and cystathionine are significantly ( P

    Techniques Used: Cell Culture, Concentration Assay

    Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.
    Figure Legend Snippet: Effects of glutathione metabolites on the growth of T. denticola . T. denticola was cultured in basic GM-1 medium (no addition) or in basic GM-1 medium containing 12 mM glutathione, 12 mM pyruvate, 12 mM H 2 S, 12 mM NH 3, 12 mM glutamate (Glu), or 12 mM glycine (Gly). OD 660 of the cultures were determined for 1 to 4 days to monitor bacterial growth. The data points and error bars indicate means and standard deviations ( n = 3). Other results (data not shown) indicated that glutamate and glycine, added either separately or in combination, had no effect on bacterial growth.

    Techniques Used: Cell Culture

    7) Product Images from "Treponema denticola in Disseminating Endodontic Infections"

    Article Title: Treponema denticola in Disseminating Endodontic Infections

    Journal:

    doi:

    Effect of endodontic infection on body weight in immunocompetent and immunodeficient mice on day 0 and day 21 after infection. SCID mice infected with T. denticola showed a significant decrease of body weight from days 0 to 21. * p
    Figure Legend Snippet: Effect of endodontic infection on body weight in immunocompetent and immunodeficient mice on day 0 and day 21 after infection. SCID mice infected with T. denticola showed a significant decrease of body weight from days 0 to 21. * p

    Techniques Used: Infection, Mouse Assay

    8) Product Images from "The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial"

    Article Title: The effect of supragingival glycine air polishing on periodontitis during maintenance therapy: a randomized controlled trial

    Journal: PeerJ

    doi: 10.7717/peerj.4371

    The prevalence of four periodontal pathogens at different time points. SGAP group, supragingival glycine air polishing group; SUSP group, supragingival ultrasonic scaling group. (A) P. ginigvalis , (B) T. forsythia , (C) T. denticola , (D) F. nucleatum .
    Figure Legend Snippet: The prevalence of four periodontal pathogens at different time points. SGAP group, supragingival glycine air polishing group; SUSP group, supragingival ultrasonic scaling group. (A) P. ginigvalis , (B) T. forsythia , (C) T. denticola , (D) F. nucleatum .

    Techniques Used:

    9) Product Images from "Dentipain, a Streptococcus-pyogenes-IdeS-protease homologue, is a novel virulence factor of Treponema denticola"

    Article Title: Dentipain, a Streptococcus-pyogenes-IdeS-protease homologue, is a novel virulence factor of Treponema denticola

    Journal: Biological chemistry

    doi: 10.1515/BC.2010.113

    Construction of an IdeT protease-deficient mutant A . (i) The wild-type ideT locus detailing the four protein domains; (ii) pIDEKO4, constructed using pMCL191, two ideT PCR generated fragments flanking the proteolytic domain (hatched bar and dotted bar) and an erythromycin resistance cassette derived from pVA2198. The restriction sites introduced during PCR are also shown; (iii) the ideT mutant locus detailing the dentipain region replaced with an erythromycin resistance cassette by homologous recombination. B . (i) PCR amplification of the ideT locus using primers ID-1 and ID-4, which amplify from amino acid residues 172 to 640. Lane 1, T. denticola ATCC 35405; lane 2, T. denticola IDEKO4; lane 3, T. denticola Ide5; lane 4, T. denticola Ide6; lane 5 , Molecular size marker. (ii) PCR amplification of the ideT locus using primers ID-1 and CATU, which amplify from amino acid residues 172 to 417. Lane 1, T. denticola ATCC 35405; lane 2, T. denticola IDEKO4; lane 3 , Molecular size marker.
    Figure Legend Snippet: Construction of an IdeT protease-deficient mutant A . (i) The wild-type ideT locus detailing the four protein domains; (ii) pIDEKO4, constructed using pMCL191, two ideT PCR generated fragments flanking the proteolytic domain (hatched bar and dotted bar) and an erythromycin resistance cassette derived from pVA2198. The restriction sites introduced during PCR are also shown; (iii) the ideT mutant locus detailing the dentipain region replaced with an erythromycin resistance cassette by homologous recombination. B . (i) PCR amplification of the ideT locus using primers ID-1 and ID-4, which amplify from amino acid residues 172 to 640. Lane 1, T. denticola ATCC 35405; lane 2, T. denticola IDEKO4; lane 3, T. denticola Ide5; lane 4, T. denticola Ide6; lane 5 , Molecular size marker. (ii) PCR amplification of the ideT locus using primers ID-1 and CATU, which amplify from amino acid residues 172 to 417. Lane 1, T. denticola ATCC 35405; lane 2, T. denticola IDEKO4; lane 3 , Molecular size marker.

    Techniques Used: Mutagenesis, Construct, Polymerase Chain Reaction, Generated, Derivative Assay, Homologous Recombination, Amplification, Marker

    Analysis of IdeT translation A. SDS-PAGE of T. denticola sonicates. Lane 1, T. denticola ATCC 35405 sonicate; lane 2, T. denticola IDEKO4 sonicate. B. Immunoblot analysis of IdeT from T. denticola cells. Lane 1, T. denticola ATCC 35405 sonicate; lane 2, T. denticola IDEKO4 sonicate. Antibody: rabbit serum anti-IdeT recombinant protein (219–647). The arrow indicates the band which reacted with this antibody. C. SDS-PAGE of concentrated culture supernatant of T. denticola . Culture supernatant of T. denticola grown in E-TYGV medium was collected and concentrated. Lane 1, culture supernatant of T. denticola ATCC 35405; lane 2, culture supernatant of T. denticola IDEKO4. D. Immunoblot analysis of IdeT in culture supernatants. Lane 1, culture supernatant of T. denticola ATCC 35405; lane 2, culture supernatant of T. denticola IDEKO4. Antibody: rabbit serum anti-IdeT recombinant protein (219–647). The arrow indicates the band which reacted with this antibody. E. Immunoblot analysis of IdeT in culture supernatants. Lane 1, culture supernatant of T. denticola ATCC 3540 5; lane 2, culture supernatant of T. denticola IDEK O4. Antibody : rabbit serum anti-IdeT synthetic peptide (IdeT498–511). The arrow indicates the band which reacted with this antibody.
    Figure Legend Snippet: Analysis of IdeT translation A. SDS-PAGE of T. denticola sonicates. Lane 1, T. denticola ATCC 35405 sonicate; lane 2, T. denticola IDEKO4 sonicate. B. Immunoblot analysis of IdeT from T. denticola cells. Lane 1, T. denticola ATCC 35405 sonicate; lane 2, T. denticola IDEKO4 sonicate. Antibody: rabbit serum anti-IdeT recombinant protein (219–647). The arrow indicates the band which reacted with this antibody. C. SDS-PAGE of concentrated culture supernatant of T. denticola . Culture supernatant of T. denticola grown in E-TYGV medium was collected and concentrated. Lane 1, culture supernatant of T. denticola ATCC 35405; lane 2, culture supernatant of T. denticola IDEKO4. D. Immunoblot analysis of IdeT in culture supernatants. Lane 1, culture supernatant of T. denticola ATCC 35405; lane 2, culture supernatant of T. denticola IDEKO4. Antibody: rabbit serum anti-IdeT recombinant protein (219–647). The arrow indicates the band which reacted with this antibody. E. Immunoblot analysis of IdeT in culture supernatants. Lane 1, culture supernatant of T. denticola ATCC 3540 5; lane 2, culture supernatant of T. denticola IDEK O4. Antibody : rabbit serum anti-IdeT synthetic peptide (IdeT498–511). The arrow indicates the band which reacted with this antibody.

    Techniques Used: SDS Page, Recombinant

    Analysis of the role of IdeT proteolytic activity in the virulence of T. denticola . Bars represent the mean area of lesions at the infection sites following challenge with either T. denticola ATCC 35405 or T. denticola IDEKO4. Mice were injected with live T. denticola ATCC 35405 (black bars) or IDEKO4 (open bars), and lesion areas determined at the indicated times following infection. Bars indicate standard deviation. *P
    Figure Legend Snippet: Analysis of the role of IdeT proteolytic activity in the virulence of T. denticola . Bars represent the mean area of lesions at the infection sites following challenge with either T. denticola ATCC 35405 or T. denticola IDEKO4. Mice were injected with live T. denticola ATCC 35405 (black bars) or IDEKO4 (open bars), and lesion areas determined at the indicated times following infection. Bars indicate standard deviation. *P

    Techniques Used: Activity Assay, Infection, Mouse Assay, Injection, Standard Deviation

    10) Product Images from "Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes"

    Article Title: Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0307639101

    Circular representation of the T. denticola (ATCC 35405) overall genome structure. The outer scale designates coordinates in base pairs. The first circle shows predicted coding regions on the plus strand color-coded by role categories: violet, amino acid biosynthesis; light blue, biosynthesis of cofactors, prosthetic groups, and carriers; light green, cell envelope; red, cellular processes; brown, central intermediary metabolism; yellow, DNA metabolism; light gray, energy metabolism; magenta, fatty acid and phospholipid metabolism; pink, protein synthesis and fate; orange, purines, pyrimidines, nucleosides, and nucleotides; olive, regulatory functions and signal transduction; dark green, transcription; teal, transport and binding proteins; gray, unknown function; salmon, other categories; blue, hypothetical proteins. The second circle shows predicted coding regions on the minus strand color-coded by role categories. The third circle shows the core set of CDSs conserved in all other sequenced spirochete genomes. The fourth circle shows CDSs with best matches to predicted CDSs in T. pallidum . The fifth circle shows putative phage regions and isolated phage genes. The sixth circle shows IS elements in black. The seventh circle shows rRNA genes in black and tRNA genes in red. The eighth circle shows trinucleotide composition in black. The ninth circle shows percentage G + C in relation to the mean G + C in a 2,000-bp window. The 10th circle shows GC-skew curve in red (positive residues) and blue (negative residues).
    Figure Legend Snippet: Circular representation of the T. denticola (ATCC 35405) overall genome structure. The outer scale designates coordinates in base pairs. The first circle shows predicted coding regions on the plus strand color-coded by role categories: violet, amino acid biosynthesis; light blue, biosynthesis of cofactors, prosthetic groups, and carriers; light green, cell envelope; red, cellular processes; brown, central intermediary metabolism; yellow, DNA metabolism; light gray, energy metabolism; magenta, fatty acid and phospholipid metabolism; pink, protein synthesis and fate; orange, purines, pyrimidines, nucleosides, and nucleotides; olive, regulatory functions and signal transduction; dark green, transcription; teal, transport and binding proteins; gray, unknown function; salmon, other categories; blue, hypothetical proteins. The second circle shows predicted coding regions on the minus strand color-coded by role categories. The third circle shows the core set of CDSs conserved in all other sequenced spirochete genomes. The fourth circle shows CDSs with best matches to predicted CDSs in T. pallidum . The fifth circle shows putative phage regions and isolated phage genes. The sixth circle shows IS elements in black. The seventh circle shows rRNA genes in black and tRNA genes in red. The eighth circle shows trinucleotide composition in black. The ninth circle shows percentage G + C in relation to the mean G + C in a 2,000-bp window. The 10th circle shows GC-skew curve in red (positive residues) and blue (negative residues).

    Techniques Used: Transduction, Binding Assay, Isolation

    Venn diagram showing the number of T. denticola predicted proteins with significant homology ( E
    Figure Legend Snippet: Venn diagram showing the number of T. denticola predicted proteins with significant homology ( E

    Techniques Used:

    11) Product Images from "Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿"

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    Journal: Infection and Immunity

    doi: 10.1128/IAI.00019-07

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.
    Figure Legend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Techniques Used: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.
    Figure Legend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Techniques Used: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Related Articles

    Mutagenesis:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Construction of a tepA2 mutant As TepA2 showed similarity to ImmA, a tepA2- deficient mutant of T. denticola ATCC 35405 was constructed by allelic exchange mutation to investigate the role of tepA2 . .. Briefly, two fragments flanking the tepA2 gene were amplified with primer pairs 718D/719U and 719D/720U (listed in Table ), respectively.

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. T. denticola ATCC 35405 ( ) and isogenic msp mutant strain MHE ( ) were grown in NOS broth medium as previously described ( , ), with erythromycin (Em) (40 μg ml−1 ) added as appropriate. .. Cultures were examined by dark-field microscopy for purity and typical strain morphology.

    Sequencing:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Sequence homology-based screening The whole genome sequence of T. denticola ATCC 35405 in the Los Alamos oral pathogen database ( http://www.oralgen.org ) was screened for homologous sequences with the S. mutans bacteriocin immunity protein (ImmA/Bip) sequence [ ] using the protein blast program. .. The obtained homologous sequences were further compared against the database of National Center for Biotechnology Information (NCBI, http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

    other:

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis
    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Construct:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Construction of a tepA2 mutant As TepA2 showed similarity to ImmA, a tepA2- deficient mutant of T. denticola ATCC 35405 was constructed by allelic exchange mutation to investigate the role of tepA2 . .. Briefly, two fragments flanking the tepA2 gene were amplified with primer pairs 718D/719U and 719D/720U (listed in Table ), respectively.

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  • 85
    ATCC t denticola draft assemblies
    Phylogeny of T. pedis isolates and T. <t>denticola</t> strains. Phylogeny based on neighbor-joining of the intergenic spacer region between the 16S rRNA and tRNA Ile . The sequences were extracted from the WGS assemblies generated in this study. (A) Phylogeny of the T. pedis isolates. (B) Phylogeny of the T. denticola strains. The bars corresponds to 0.015 and 0.050 nucleotide substitutions per position.
    T Denticola Draft Assemblies, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC t denticola cells
    Antibodies to rMsp inhibit T. <t>denticola</t> cell adhesion.
    T Denticola Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC t denticola cke
    Role of the CTLP complex in the formation of T. <t>denticola</t> / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola <t>CKE,</t> with P. gingivalis ATCC 33277. Biofilms were
    T Denticola Cke, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    ATCC t denticola biology
    Chromosomal integration of the full-length flgE into T. <t>denticola</t> . The integration construct containing the full-length flgE gene and the kanamycin cassette was integrated into the genome of the flgE mutant. The expressions of flgE and flaA were evaluated.
    T Denticola Biology, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Phylogeny of T. pedis isolates and T. denticola strains. Phylogeny based on neighbor-joining of the intergenic spacer region between the 16S rRNA and tRNA Ile . The sequences were extracted from the WGS assemblies generated in this study. (A) Phylogeny of the T. pedis isolates. (B) Phylogeny of the T. denticola strains. The bars corresponds to 0.015 and 0.050 nucleotide substitutions per position.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Phylogeny of T. pedis isolates and T. denticola strains. Phylogeny based on neighbor-joining of the intergenic spacer region between the 16S rRNA and tRNA Ile . The sequences were extracted from the WGS assemblies generated in this study. (A) Phylogeny of the T. pedis isolates. (B) Phylogeny of the T. denticola strains. The bars corresponds to 0.015 and 0.050 nucleotide substitutions per position.

    Article Snippet: The T. denticola draft assemblies contributed 977 clusters with no BLASTP hit in T. denticola ATCC 35405 ( ).

    Techniques: Generated

    Quantification of strain-specific, intermediately-represented, and core genes in T. pedis and T. denticola . The distribution between strain-specific genes, intermediately-represented genes, and genes present in all genomes, i.e., core functions, in the analyzed genome datasets of T. pedis (A) and T. denticola (B). The gene representation was determined by a clustering analysis that collapsed CDSs sharing > 80% BLASTP identity and that had

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Quantification of strain-specific, intermediately-represented, and core genes in T. pedis and T. denticola . The distribution between strain-specific genes, intermediately-represented genes, and genes present in all genomes, i.e., core functions, in the analyzed genome datasets of T. pedis (A) and T. denticola (B). The gene representation was determined by a clustering analysis that collapsed CDSs sharing > 80% BLASTP identity and that had

    Article Snippet: The T. denticola draft assemblies contributed 977 clusters with no BLASTP hit in T. denticola ATCC 35405 ( ).

    Techniques:

    Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Journal: PLoS ONE

    Article Title: Genome-Wide Relatedness of Treponema pedis, from Gingiva and Necrotic Skin Lesions of Pigs, with the Human Oral Pathogen Treponema denticola

    doi: 10.1371/journal.pone.0071281

    Figure Lengend Snippet: Circular representation of the T. pedis T A4 genome and complete genome alignment with T. denticola . (A.) Circular representation of the T. pedis T A4 genome. The CDSs are shown in violet where the outer circle represents predictions on the plus strand and the second circle those on the minus strand. CDSs with a best BLASTP hit in T. denticola ATCC 35405 are colored red and shown in the third circle. The fourth circle represents genes with best BLASTP hits in T. brennaborense (black), F. nucleatum (green), F. alocis (blue) and T. succinifaciens (grey). G+C skew is drawn in the inner circle. (B.) Complete genome alignment between T. pedis T A4 and T. denticola ATCC 35405. Dots represent maximum unique matches (MUMs) between the genomes. MUMs oriented in the same direction are depicted as red dots and reverse complemented MUMs are depicted as blue dots.

    Article Snippet: The T. denticola draft assemblies contributed 977 clusters with no BLASTP hit in T. denticola ATCC 35405 ( ).

    Techniques:

    Antibodies to rMsp inhibit T. denticola cell adhesion.

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Antibodies to rMsp inhibit T. denticola cell adhesion.

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques:

    Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Interactions of rMsp antibodies with T. denticola ATCC 35405 cells and of whole-cell antibodies with rMsp fragments. (Panel A) Reactivity in ELISA of antiserum (1:250 diluted) to T. denticola ATCC 35405 cells (W) or antisera (1:250 diluted) raised to

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques: Enzyme-linked Immunosorbent Assay

    Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Reactivities of Msp antisera with T. denticola ATCC 35405 outer membrane protein extracts. (Panel A) SDS-PAGE gel of outer membrane proteins. (Panel B) Western blots reacted with antisera to rN-Msp (lane 1), rV-Msp (lane 2), or rC-Msp (lane 3). Molecular

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques: SDS Page, Western Blot

    Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Binding of rMsp to human fibronectin and to 30-kDa N-terminal fragment of human fibronectin. (Panel A) Western blot overlay of trypsin-derived fragments of human plasma fibronectin reacted with rMsp (lane 1) or with biotinylated T. denticola ATCC 35405

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques: Binding Assay, Western Blot, Derivative Assay

    Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Diagrammatic representation of the Msp sequences from T. denticola ATCC 35520 (A), strain ATCC 35405 (B), and of recombinant Msp polypeptides rMsp (530 aa residues), rN-Msp (189 aa residues), rV-Msp (57 aa residues), and rN-Msp (272 aa residues), with

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques: Recombinant

    Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin

    Journal: Infection and Immunity

    Article Title: Binding Properties and Adhesion-Mediating Regions of the Major Sheath Protein of Treponema denticola ATCC 35405

    doi: 10.1128/IAI.73.5.2891-2898.2005

    Figure Lengend Snippet: Effect of exogenously added rMsp on adhesion of Treponema strains to immobilized human fibronectin or 30-kDa fibronectin fragment. (Panel A) Adhesion levels of T. denticola ATCC 35405 cells to 0.1 μg fibronectin (filled column) or 30-kDa fibronectin

    Article Snippet: These fibronectin interaction properties of Msp are similar to those of T. denticola cells and to those of T. vincentii and related Treponema species ( ). rMsp was found to block, by up to 50%, binding of T. denticola ATCC 35405 cells to fibronectin, an observation that conflicts with a previous report showing that full-length recombinant Msp did not significantly affect adhesion to fibronectin ( ).

    Techniques:

    Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Role of the CTLP complex in the formation of T. denticola / P. gingivalis dual-species biofilms. Representative CLSM images of dual-species biofilms of (a, b) T. denticola ATCC 35405 and (c, d) T. denticola CKE, with P. gingivalis ATCC 33277. Biofilms were

    Article Snippet: Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed.

    Techniques: Confocal Laser Scanning Microscopy

    Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Adherence levels of Treponema to other periodontopathogens. The input cell number was 1.2×10 7 cells per well of T. denticola ATCC 35405 (black bars), T. denticola MHE (mid-grey bars), T. denticola CKE (dark-grey bars) and T. vincentii ATCC 35580

    Article Snippet: Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed.

    Techniques:

    Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Journal: Microbiology

    Article Title: Treponema denticola chymotrypsin-like proteinase (CTLP) integrates spirochaetes within oral microbial communities

    doi: 10.1099/mic.0.055939-0

    Figure Lengend Snippet: Fluorescence microscopy of monospecies or dual-species biofilms of T. denticola ATCC 35405, T. denticola CKE or T. denticola MHE, and P. gingivalis ATCC 33277, and corresponding biomass data. (a) Biofilms formed upon saliva-coated coverslips after 72

    Article Snippet: Accordingly, chymotrypsin-like activities associated with T. denticola ATCC 35405, T. denticola MHE, T. denticola CKE and T. vincentii ATCC 35580 were assessed.

    Techniques: Fluorescence, Microscopy

    Chromosomal integration of the full-length flgE into T. denticola . The integration construct containing the full-length flgE gene and the kanamycin cassette was integrated into the genome of the flgE mutant. The expressions of flgE and flaA were evaluated.

    Journal: Applied and Environmental Microbiology

    Article Title: Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola

    doi: 10.1128/AEM.00478-15

    Figure Lengend Snippet: Chromosomal integration of the full-length flgE into T. denticola . The integration construct containing the full-length flgE gene and the kanamycin cassette was integrated into the genome of the flgE mutant. The expressions of flgE and flaA were evaluated.

    Article Snippet: Efforts to further our understanding of T. denticola biology have been impeded by the fastidious nature and specific genetic restriction modification system of this oral spirochete, particularly in the widely used strain T. denticola ATCC 35405, which has a sequenced and fully annotated genome ( , , ).

    Techniques: Construct, Mutagenesis

    Characterization of T. denticola variants. (A) Protein profile of T. denticola . Spirochetal cell lysates from exponentially grown T. denticola variants were prepared and applied to SDS–12% PAGE, followed by Coomassie blue staining. Arrows represent

    Journal: Applied and Environmental Microbiology

    Article Title: Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola

    doi: 10.1128/AEM.00478-15

    Figure Lengend Snippet: Characterization of T. denticola variants. (A) Protein profile of T. denticola . Spirochetal cell lysates from exponentially grown T. denticola variants were prepared and applied to SDS–12% PAGE, followed by Coomassie blue staining. Arrows represent

    Article Snippet: Efforts to further our understanding of T. denticola biology have been impeded by the fastidious nature and specific genetic restriction modification system of this oral spirochete, particularly in the widely used strain T. denticola ATCC 35405, which has a sequenced and fully annotated genome ( , , ).

    Techniques: Polyacrylamide Gel Electrophoresis, Staining

    Screening of antibiotic resistance cassettes for T. denticola 35405.

    Journal: Applied and Environmental Microbiology

    Article Title: Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola

    doi: 10.1128/AEM.00478-15

    Figure Lengend Snippet: Screening of antibiotic resistance cassettes for T. denticola 35405.

    Article Snippet: Efforts to further our understanding of T. denticola biology have been impeded by the fastidious nature and specific genetic restriction modification system of this oral spirochete, particularly in the widely used strain T. denticola ATCC 35405, which has a sequenced and fully annotated genome ( , , ).

    Techniques:

    Schematic diagram of plasmid constructs for targeted mutagenesis and integration of flgE into T. denticola 35405. (A) Plasmid construction for replacement of TDE1430 with antibiotic cassette driven by a promoter. Plasmids (VI, VII, VIII, IX, and X) were

    Journal: Applied and Environmental Microbiology

    Article Title: Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola

    doi: 10.1128/AEM.00478-15

    Figure Lengend Snippet: Schematic diagram of plasmid constructs for targeted mutagenesis and integration of flgE into T. denticola 35405. (A) Plasmid construction for replacement of TDE1430 with antibiotic cassette driven by a promoter. Plasmids (VI, VII, VIII, IX, and X) were

    Article Snippet: Efforts to further our understanding of T. denticola biology have been impeded by the fastidious nature and specific genetic restriction modification system of this oral spirochete, particularly in the widely used strain T. denticola ATCC 35405, which has a sequenced and fully annotated genome ( , , ).

    Techniques: Plasmid Preparation, Construct, Mutagenesis

    Chromosomal integration of flgE restored PF and motility. (A) PF of T. denticola were recovered after the integration of flgE into T. denticola genome. Electron microscopic examination of exposed PF (arrow) from spirochetal cells treated with 1% Triton

    Journal: Applied and Environmental Microbiology

    Article Title: Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola

    doi: 10.1128/AEM.00478-15

    Figure Lengend Snippet: Chromosomal integration of flgE restored PF and motility. (A) PF of T. denticola were recovered after the integration of flgE into T. denticola genome. Electron microscopic examination of exposed PF (arrow) from spirochetal cells treated with 1% Triton

    Article Snippet: Efforts to further our understanding of T. denticola biology have been impeded by the fastidious nature and specific genetic restriction modification system of this oral spirochete, particularly in the widely used strain T. denticola ATCC 35405, which has a sequenced and fully annotated genome ( , , ).

    Techniques: