t denticola strain atcc 35405  (ATCC)


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    Structured Review

    ATCC t denticola strain atcc 35405
    Gene pathways and volcano plot shows RASA4 as a top upregulated gene in T. <t>denticola</t> challenged PDL cells. PDL cells were unchallenged or challenged with T. denticola (50 MOI) for 24 h and differential gene expression analysis was performed using RNA-Seq. 165 genes were identified from the gene ontology pathways derived from the RNA sequencing data. Biological processes were identified from the top significant processes that were differentially regulated in the gene ontology enrichment analysis from 5 h and 24 h gene ontology graphs ( Supporting Information , Figure 3 and A ). Pathways identified were titled regulation of actin filament organization, positive regulation of cell projection organization, regulation of actin filament organization, ras protein signal transduction, regulation of small GTPase meditated signal transduction, extracellular matrix component, and extracellular matrix organization. Based on these pathways, the top genes were identified and their adjusted p-value and differential expression was plotted as a volcano plot (B) . All genes used in the volcano plot came from the 24 h challenge data sets within the pathways specified above. RASA4 was identified as one of the top genes upregulated upon 24 h T. denticola challenge.
    T Denticola Strain Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    t denticola strain atcc 35405 - by Bioz Stars, 2022-11
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    Images

    1) Product Images from "Treponema denticola-Induced RASA4 Upregulation Mediates Cytoskeletal Dysfunction and MMP-2 Activity in Periodontal Fibroblasts"

    Article Title: Treponema denticola-Induced RASA4 Upregulation Mediates Cytoskeletal Dysfunction and MMP-2 Activity in Periodontal Fibroblasts

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.671968

    Gene pathways and volcano plot shows RASA4 as a top upregulated gene in T. denticola challenged PDL cells. PDL cells were unchallenged or challenged with T. denticola (50 MOI) for 24 h and differential gene expression analysis was performed using RNA-Seq. 165 genes were identified from the gene ontology pathways derived from the RNA sequencing data. Biological processes were identified from the top significant processes that were differentially regulated in the gene ontology enrichment analysis from 5 h and 24 h gene ontology graphs ( Supporting Information , Figure 3 and A ). Pathways identified were titled regulation of actin filament organization, positive regulation of cell projection organization, regulation of actin filament organization, ras protein signal transduction, regulation of small GTPase meditated signal transduction, extracellular matrix component, and extracellular matrix organization. Based on these pathways, the top genes were identified and their adjusted p-value and differential expression was plotted as a volcano plot (B) . All genes used in the volcano plot came from the 24 h challenge data sets within the pathways specified above. RASA4 was identified as one of the top genes upregulated upon 24 h T. denticola challenge.
    Figure Legend Snippet: Gene pathways and volcano plot shows RASA4 as a top upregulated gene in T. denticola challenged PDL cells. PDL cells were unchallenged or challenged with T. denticola (50 MOI) for 24 h and differential gene expression analysis was performed using RNA-Seq. 165 genes were identified from the gene ontology pathways derived from the RNA sequencing data. Biological processes were identified from the top significant processes that were differentially regulated in the gene ontology enrichment analysis from 5 h and 24 h gene ontology graphs ( Supporting Information , Figure 3 and A ). Pathways identified were titled regulation of actin filament organization, positive regulation of cell projection organization, regulation of actin filament organization, ras protein signal transduction, regulation of small GTPase meditated signal transduction, extracellular matrix component, and extracellular matrix organization. Based on these pathways, the top genes were identified and their adjusted p-value and differential expression was plotted as a volcano plot (B) . All genes used in the volcano plot came from the 24 h challenge data sets within the pathways specified above. RASA4 was identified as one of the top genes upregulated upon 24 h T. denticola challenge.

    Techniques Used: Expressing, RNA Sequencing Assay, Derivative Assay, Transduction

    RASA4 gene expression is required for T. denticola -mediated enhancement of MMP-2 activity. Pro-MMP-2 and active-MMP-2 in scramble shRNA and RASA4shRNA cells challenged with T. denticola (50 MOI) for 24 h. (A) a representative zymogram. (B) (Pro-Mmp-2) and (C) (active Mmp-2) show mean ± SD from three independent experiments. Data was compared using Two-way ANOVA Tukey’s Multiple comparisons test. * = p
    Figure Legend Snippet: RASA4 gene expression is required for T. denticola -mediated enhancement of MMP-2 activity. Pro-MMP-2 and active-MMP-2 in scramble shRNA and RASA4shRNA cells challenged with T. denticola (50 MOI) for 24 h. (A) a representative zymogram. (B) (Pro-Mmp-2) and (C) (active Mmp-2) show mean ± SD from three independent experiments. Data was compared using Two-way ANOVA Tukey’s Multiple comparisons test. * = p

    Techniques Used: Expressing, Activity Assay, shRNA

    T. denticola challenge decreases stress fibers and β-actin protein monomer abundance in PDL cells. (A) ( Left ) PDL cells were unchallenged (control) or challenged with T. denticola (50 MOI) for 24 h and processed for immunofluorescent staining with Hoechst (nucleus, blue) and SiR-Actin probe, and imaged using confocal microscopy. ( Right ) Relative optical intensities were calculated from three independent experiments. (B) ( Left ) Representative immunoblot showing β-actin levels in PDL cells challenged with control medium or media containing T. denticola (50 MOI ) for 24 h. ( Right ), Densitometry analysis of three independent experiments was performed using ImageJ software, and the ratio of control samples to T. denticola was calculated. Data represent mean ± SD from three independent experiments. Data were compared using Student’s t-test. * = p
    Figure Legend Snippet: T. denticola challenge decreases stress fibers and β-actin protein monomer abundance in PDL cells. (A) ( Left ) PDL cells were unchallenged (control) or challenged with T. denticola (50 MOI) for 24 h and processed for immunofluorescent staining with Hoechst (nucleus, blue) and SiR-Actin probe, and imaged using confocal microscopy. ( Right ) Relative optical intensities were calculated from three independent experiments. (B) ( Left ) Representative immunoblot showing β-actin levels in PDL cells challenged with control medium or media containing T. denticola (50 MOI ) for 24 h. ( Right ), Densitometry analysis of three independent experiments was performed using ImageJ software, and the ratio of control samples to T. denticola was calculated. Data represent mean ± SD from three independent experiments. Data were compared using Student’s t-test. * = p

    Techniques Used: Staining, Confocal Microscopy, Software

    Jasplakinolide inhibited T. denticola -induced RASA4 gene expression, whereas RASA4 gene expression is upregulated in Latrunculin B pretreated and T. denticola challenged PDL cells. Quantitative RT-PCR analysis of RASA4 relative fold change in the gene expression of PDL cells treated with Jasplakinolide (Jasp; panel A ) or Latrunculin B (Lat B; panel B ) and challenged with T. denticola (50 MOI). Data represent mean ± SD from three independent experiments. Data were compared using Two-way ANOVA Tukey’s Multiple comparisons test. * = p
    Figure Legend Snippet: Jasplakinolide inhibited T. denticola -induced RASA4 gene expression, whereas RASA4 gene expression is upregulated in Latrunculin B pretreated and T. denticola challenged PDL cells. Quantitative RT-PCR analysis of RASA4 relative fold change in the gene expression of PDL cells treated with Jasplakinolide (Jasp; panel A ) or Latrunculin B (Lat B; panel B ) and challenged with T. denticola (50 MOI). Data represent mean ± SD from three independent experiments. Data were compared using Two-way ANOVA Tukey’s Multiple comparisons test. * = p

    Techniques Used: Expressing, Quantitative RT-PCR

    Purified dentilisin and T. denticola upregulate RASA4 gene expression in PDL cells. Quantitative RT-PCR analysis of RASA4 relative fold change in PDL cells challenged with V. parvula , wild type T. denticola , T. denticola dentilisin mutant CF522, T. denticola MHE or treated with purified dentilisin for 24 h. Data represent mean ± SD from three independent experiments. Data were compared using One-way ANOVA Tukey’s Multiple comparisons test. * = p
    Figure Legend Snippet: Purified dentilisin and T. denticola upregulate RASA4 gene expression in PDL cells. Quantitative RT-PCR analysis of RASA4 relative fold change in PDL cells challenged with V. parvula , wild type T. denticola , T. denticola dentilisin mutant CF522, T. denticola MHE or treated with purified dentilisin for 24 h. Data represent mean ± SD from three independent experiments. Data were compared using One-way ANOVA Tukey’s Multiple comparisons test. * = p

    Techniques Used: Purification, Expressing, Quantitative RT-PCR, Mutagenesis

    RASA4 gene expression is required for T. denticola -mediated actin stress fiber dysfunction. (A) Quantitative RT-PCR analysis of RASA4 relative fold change in PDL cells infected with scramble shRNA or RASA4 shRNA. (B) Representative confocal images and (C) relative optical intensity of stress fiber intensity in scramble shRNA, RASA4 shRNA and RASA4shRNA cells challenged cells with T. denticola (50 MOI) for 24 h and stained with SiR-Actin dye (1:5000). Data represent mean ± SD from three independent experiments. Data in panel (A) was analyzed using an Unpaired t test. Data in panel (C) was analyzed using One-way ANOVA Tukey’s Multiple comparisons test. * = p
    Figure Legend Snippet: RASA4 gene expression is required for T. denticola -mediated actin stress fiber dysfunction. (A) Quantitative RT-PCR analysis of RASA4 relative fold change in PDL cells infected with scramble shRNA or RASA4 shRNA. (B) Representative confocal images and (C) relative optical intensity of stress fiber intensity in scramble shRNA, RASA4 shRNA and RASA4shRNA cells challenged cells with T. denticola (50 MOI) for 24 h and stained with SiR-Actin dye (1:5000). Data represent mean ± SD from three independent experiments. Data in panel (A) was analyzed using an Unpaired t test. Data in panel (C) was analyzed using One-way ANOVA Tukey’s Multiple comparisons test. * = p

    Techniques Used: Expressing, Quantitative RT-PCR, Infection, shRNA, Staining

    PDL cell attachment and contractility is impaired by T. denticola challenge. PDL cells were unchallenged (control) or challenged with T. denticola (50 MOI) for 24 h and processed for immunofluorescent staining with Hoechst (nucleus, blue), and imaged with Confocal Microscopy. Representative phase contrast images are shown, and graphs show the percent change in de-adhesion after 24 h. Data represent mean ± SD from three independent experiments. Data were compared using Student’s t-test. *** = p
    Figure Legend Snippet: PDL cell attachment and contractility is impaired by T. denticola challenge. PDL cells were unchallenged (control) or challenged with T. denticola (50 MOI) for 24 h and processed for immunofluorescent staining with Hoechst (nucleus, blue), and imaged with Confocal Microscopy. Representative phase contrast images are shown, and graphs show the percent change in de-adhesion after 24 h. Data represent mean ± SD from three independent experiments. Data were compared using Student’s t-test. *** = p

    Techniques Used: Cell Attachment Assay, Staining, Confocal Microscopy

    Actin depolymerization through T. denticola challenge and Latrunculin B pretreatment increases MMP-2 enzymatic activity. Actin polymerizing agent Jasplakinolide (Jasp) and actin depolymerizing agent Latrunculin B (Lat B) were used to pretreat PDL cells for one h. Cells were then unchallenged or challenged with T. denticola (50 MOI) for 24 h. Cultured media was collected and gelatin zymography was used to measure MMP-2 activity. A representative Gelatin zymogram is shown (A) and densitometry analysis of pro-MMP-2 (B) and active-MMP-2 (C) . Data represent mean ± SD from three independent experiments. Data were compared using One-way ANOVA Tukey’s Multiple comparisons test. *. = p
    Figure Legend Snippet: Actin depolymerization through T. denticola challenge and Latrunculin B pretreatment increases MMP-2 enzymatic activity. Actin polymerizing agent Jasplakinolide (Jasp) and actin depolymerizing agent Latrunculin B (Lat B) were used to pretreat PDL cells for one h. Cells were then unchallenged or challenged with T. denticola (50 MOI) for 24 h. Cultured media was collected and gelatin zymography was used to measure MMP-2 activity. A representative Gelatin zymogram is shown (A) and densitometry analysis of pro-MMP-2 (B) and active-MMP-2 (C) . Data represent mean ± SD from three independent experiments. Data were compared using One-way ANOVA Tukey’s Multiple comparisons test. *. = p

    Techniques Used: Activity Assay, Cell Culture, Zymography

    2) Product Images from "Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression in Treponema denticola"

    Article Title: Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression in Treponema denticola

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.668287

    The dentilisin operon in  T. denticola  ATCC 35405. The three genes of the protease operon are expressed as a single mRNA transcript. PrtP is expressed as a preproprotein which is activated upon removal of the prepropeptide (N-PrtP). Asterisks (*) indicate predicted N-terminal acylation. PrtP is required for processing of PrcA to PrcA1 and PrcA2. [adapted and modified from (  Godovikova, Goetting-Minesky et al. 2011 )].
    Figure Legend Snippet: The dentilisin operon in T. denticola ATCC 35405. The three genes of the protease operon are expressed as a single mRNA transcript. PrtP is expressed as a preproprotein which is activated upon removal of the prepropeptide (N-PrtP). Asterisks (*) indicate predicted N-terminal acylation. PrtP is required for processing of PrcA to PrcA1 and PrcA2. [adapted and modified from ( Godovikova, Goetting-Minesky et al. 2011 )].

    Techniques Used: Modification

    Composite immunogold transmission electron micrograph showing a T. denticola ATCC 35405 dividing cell labelled with rabbit immunoglobulins raised against the dentilisin protease complex followed by gold-conjugated anti-rabbit IgG, as reported previously ( Grenier et al., 1990 ). The hexagonal array of the Msp complex is visible in the outer membrane. The inset shows an enlargement of the indicated region. Bar: 300 nm. [reprinted with permission from ( Fenno, 2011 )].
    Figure Legend Snippet: Composite immunogold transmission electron micrograph showing a T. denticola ATCC 35405 dividing cell labelled with rabbit immunoglobulins raised against the dentilisin protease complex followed by gold-conjugated anti-rabbit IgG, as reported previously ( Grenier et al., 1990 ). The hexagonal array of the Msp complex is visible in the outer membrane. The inset shows an enlargement of the indicated region. Bar: 300 nm. [reprinted with permission from ( Fenno, 2011 )].

    Techniques Used: Transmission Assay

    3) Product Images from "Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression in Treponema denticola"

    Article Title: Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression in Treponema denticola

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.668287

    The dentilisin operon in T. denticola ATCC 35405. The three genes of the protease operon are expressed as a single mRNA transcript. PrtP is expressed as a preproprotein which is activated upon removal of the prepropeptide (N-PrtP). Asterisks (*) indicate predicted N-terminal acylation. PrtP is required for processing of PrcA to PrcA1 and PrcA2. [adapted and modified from ( Godovikova, Goetting-Minesky et al. 2011 )].
    Figure Legend Snippet: The dentilisin operon in T. denticola ATCC 35405. The three genes of the protease operon are expressed as a single mRNA transcript. PrtP is expressed as a preproprotein which is activated upon removal of the prepropeptide (N-PrtP). Asterisks (*) indicate predicted N-terminal acylation. PrtP is required for processing of PrcA to PrcA1 and PrcA2. [adapted and modified from ( Godovikova, Goetting-Minesky et al. 2011 )].

    Techniques Used: Modification

    Composite immunogold transmission electron micrograph showing a T. denticola ATCC 35405 dividing cell labelled with rabbit immunoglobulins raised against the dentilisin protease complex followed by gold-conjugated anti-rabbit IgG, as reported previously ( Grenier et al., 1990 ). The hexagonal array of the Msp complex is visible in the outer membrane. The inset shows an enlargement of the indicated region. Bar: 300 nm. [reprinted with permission from ( Fenno, 2011 )].
    Figure Legend Snippet: Composite immunogold transmission electron micrograph showing a T. denticola ATCC 35405 dividing cell labelled with rabbit immunoglobulins raised against the dentilisin protease complex followed by gold-conjugated anti-rabbit IgG, as reported previously ( Grenier et al., 1990 ). The hexagonal array of the Msp complex is visible in the outer membrane. The inset shows an enlargement of the indicated region. Bar: 300 nm. [reprinted with permission from ( Fenno, 2011 )].

    Techniques Used: Transmission Assay

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    ATCC t denticola strain atcc 35405
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