t denticola strains atcc 35405  (ATCC)


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    Name:
    Treponema denticola a CIP 103919 DSM 14222
    Description:

    Catalog Number:
    35405
    Price:
    None
    Applications:
    Produces major surface proteinProduces methyl-accepting chemotaxis proteinProduces prolyl aminopeptidase proline iminopeptidase
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    Structured Review

    ATCC t denticola strains atcc 35405
    Southern blot analysis of tepA1 ( a ), tepA2 ( b ) , and tepA3 ( c ). Genomic DNA from T. <t>denticola</t> strains was digested with Hin dIII. 1: genomic DNA from ATCC 33520, 2: genomic DNA from ATCC 33521, genomic DNA from ATCC 35404, 4: genomic DNA from ATCC 35405, 5: genomic DNA from GM1

    https://www.bioz.com/result/t denticola strains atcc 35405/product/ATCC
    Average 99 stars, based on 2 article reviews
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    Images

    1) Product Images from "Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola"

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola

    Journal: BMC Oral Health

    doi: 10.1186/s12903-016-0243-7

    Southern blot analysis of tepA1 ( a ), tepA2 ( b ) , and tepA3 ( c ). Genomic DNA from T. denticola strains was digested with Hin dIII. 1: genomic DNA from ATCC 33520, 2: genomic DNA from ATCC 33521, genomic DNA from ATCC 35404, 4: genomic DNA from ATCC 35405, 5: genomic DNA from GM1
    Figure Legend Snippet: Southern blot analysis of tepA1 ( a ), tepA2 ( b ) , and tepA3 ( c ). Genomic DNA from T. denticola strains was digested with Hin dIII. 1: genomic DNA from ATCC 33520, 2: genomic DNA from ATCC 33521, genomic DNA from ATCC 35404, 4: genomic DNA from ATCC 35405, 5: genomic DNA from GM1

    Techniques Used: Southern Blot

    2) Product Images from "The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis"

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis

    Journal: BMC Infectious Diseases

    doi: 10.1186/1471-2334-10-345

    MSP amino acid sequence alignment of T. denticola strains ATCC 35405 and Group A (panel A), and ATCC 33520 and Group B (panel B) . The grey areas indicate single amino acid substitutions compared with ATCC 35405 (panel A) and ATCC 33520 (panel B).
    Figure Legend Snippet: MSP amino acid sequence alignment of T. denticola strains ATCC 35405 and Group A (panel A), and ATCC 33520 and Group B (panel B) . The grey areas indicate single amino acid substitutions compared with ATCC 35405 (panel A) and ATCC 33520 (panel B).

    Techniques Used: Sequencing

    Diversity of msp sequences . Sequence alignments of 17 central regions (from 600 to 900 nucleotides) from T. denticola positive clinical specimens. In panel A are shown the clinical samples of Group A. In panel B are shown the clinical samples of Group B. The upper line contains the sequence of T. denticola strain ATCC 35405 and ATCC 33520, respectively, in both panels. The grey areas indicate variations of single nucleotide positions compared with T. denticola ATCC 35405.
    Figure Legend Snippet: Diversity of msp sequences . Sequence alignments of 17 central regions (from 600 to 900 nucleotides) from T. denticola positive clinical specimens. In panel A are shown the clinical samples of Group A. In panel B are shown the clinical samples of Group B. The upper line contains the sequence of T. denticola strain ATCC 35405 and ATCC 33520, respectively, in both panels. The grey areas indicate variations of single nucleotide positions compared with T. denticola ATCC 35405.

    Techniques Used: Sequencing

    Evolutionary relationships of T. denticola msp gene, deduced from the sequences of 17 clinical samples, ATCC 35405, ATCC 33520, and OTK . The phylogenetic analysis was performed using the neighbor-joining method with MEGA 4 on aligned sequences from the msp complete cds sequence (bootstrap values > 75 are shown at nodes).
    Figure Legend Snippet: Evolutionary relationships of T. denticola msp gene, deduced from the sequences of 17 clinical samples, ATCC 35405, ATCC 33520, and OTK . The phylogenetic analysis was performed using the neighbor-joining method with MEGA 4 on aligned sequences from the msp complete cds sequence (bootstrap values > 75 are shown at nodes).

    Techniques Used: Sequencing

    Antigenicity plot of T. denticola strains ATCC 35405 (frame A), ATCC 33520 (frame B), and 2 representative specimens (B66, frame C; B23, frame D) . In each frame, the area of the plot that is surrounded by a continuous line represents the central region of MSP. The internal smaller area, surrounded by the dotted line, represents the portion that has the greatest difference in predicted antigenicity between specimens B66 and B23 and ATCC35405.
    Figure Legend Snippet: Antigenicity plot of T. denticola strains ATCC 35405 (frame A), ATCC 33520 (frame B), and 2 representative specimens (B66, frame C; B23, frame D) . In each frame, the area of the plot that is surrounded by a continuous line represents the central region of MSP. The internal smaller area, surrounded by the dotted line, represents the portion that has the greatest difference in predicted antigenicity between specimens B66 and B23 and ATCC35405.

    Techniques Used:

    3) Product Images from "Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)"

    Article Title: Immunotopological Analysis of the Treponema denticola Major Surface Protein (Msp)

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00528-18

    Immunofluorescence microscopy of T. denticola , revealing the Msp surface domain. T. denticola ATCC 35405 and ATCC 33520, grown to an OD 600 of 0.2, were fixed on glass slides with 1% paraformaldehyde, incubated with PBS or PBS plus 0.5% Triton X-100, and probed with rabbit antibodies to native ATCC 35405 Msp or recombinant FlaA, followed by goat anti-rabbit IgG conjugated to Alexa Fluor 568 (Msp) or Alexa Fluor 488 (FlaA), or with DAPI. Images were obtained at a magnification of 600× using an Olympus BX40 microscope fitted with an Olympus DP73 camera.
    Figure Legend Snippet: Immunofluorescence microscopy of T. denticola , revealing the Msp surface domain. T. denticola ATCC 35405 and ATCC 33520, grown to an OD 600 of 0.2, were fixed on glass slides with 1% paraformaldehyde, incubated with PBS or PBS plus 0.5% Triton X-100, and probed with rabbit antibodies to native ATCC 35405 Msp or recombinant FlaA, followed by goat anti-rabbit IgG conjugated to Alexa Fluor 568 (Msp) or Alexa Fluor 488 (FlaA), or with DAPI. Images were obtained at a magnification of 600× using an Olympus BX40 microscope fitted with an Olympus DP73 camera.

    Techniques Used: Immunofluorescence, Microscopy, Incubation, Recombinant

    Map and immunoblots of deletion mutations made in msp for expression in E. coli . (Upper) The 543-residue Msp protein, with the signal peptide (SP) cleavage site indicated (residue 20), is shown above the deletions made in the coding region. The amino acid residues deleted in the N-terminal region in each of the three constructs are indicated. Deletions in the central region within the approximate boundaries of the central domain (D1 through D2) include predicted antigenic domains (Ag1 and Ag2), the remaining region of greatest divergence between Msps of strains ATCC 35405 and ATCC 33520 (Xr), and D2. These mutant Msps were expressed in E. coli . (Lower) Immunoblots of E. coli strains expressing full-length Msps with the indicated Msp residues deleted are shown. T. denticola ATCC 35405 serves as a positive control. Blots were probed with rabbit polyclonal antibodies raised against native ATCC 35405 Msp, the Msp N-terminal domain, or whole T. denticola (Td) cells. All samples were boiled prior to electrophoresis. Lanes contain lysates of E. coli strains. Antibodies raised against native T. denticola ATCC 35405 Msp or whole T. denticola ATCC 35405 cells do not recognize recombinant Msps lacking residues 229 to 251.
    Figure Legend Snippet: Map and immunoblots of deletion mutations made in msp for expression in E. coli . (Upper) The 543-residue Msp protein, with the signal peptide (SP) cleavage site indicated (residue 20), is shown above the deletions made in the coding region. The amino acid residues deleted in the N-terminal region in each of the three constructs are indicated. Deletions in the central region within the approximate boundaries of the central domain (D1 through D2) include predicted antigenic domains (Ag1 and Ag2), the remaining region of greatest divergence between Msps of strains ATCC 35405 and ATCC 33520 (Xr), and D2. These mutant Msps were expressed in E. coli . (Lower) Immunoblots of E. coli strains expressing full-length Msps with the indicated Msp residues deleted are shown. T. denticola ATCC 35405 serves as a positive control. Blots were probed with rabbit polyclonal antibodies raised against native ATCC 35405 Msp, the Msp N-terminal domain, or whole T. denticola (Td) cells. All samples were boiled prior to electrophoresis. Lanes contain lysates of E. coli strains. Antibodies raised against native T. denticola ATCC 35405 Msp or whole T. denticola ATCC 35405 cells do not recognize recombinant Msps lacking residues 229 to 251.

    Techniques Used: Western Blot, Expressing, Construct, Mutagenesis, Positive Control, Electrophoresis, Recombinant

    Models for T. denticola ATCC 35405 Msp generated by I-TASSER. The I-TASSER algorithm generates 5 predicted structural models based on several parameters, of which model 1 is the overall best model. Overall model ranking does not necessarily follow the C scores for proteins lacking closely related known structures. All 5 models are displayed to show the N termini (blue) and C termini (red). The ATCC 35405 Msp surface-exposed epitope (residues 229 to 251) is highlighted in green in each model. (A) Models 1 to 5 generated using the PDB data available in June 2017. (B) Models 1 to 5 generated using the PDB data available in October 2018.
    Figure Legend Snippet: Models for T. denticola ATCC 35405 Msp generated by I-TASSER. The I-TASSER algorithm generates 5 predicted structural models based on several parameters, of which model 1 is the overall best model. Overall model ranking does not necessarily follow the C scores for proteins lacking closely related known structures. All 5 models are displayed to show the N termini (blue) and C termini (red). The ATCC 35405 Msp surface-exposed epitope (residues 229 to 251) is highlighted in green in each model. (A) Models 1 to 5 generated using the PDB data available in June 2017. (B) Models 1 to 5 generated using the PDB data available in October 2018.

    Techniques Used: Generated

    Related Articles

    Mutagenesis:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Construction of a tepA2 mutant As TepA2 showed similarity to ImmA, a tepA2- deficient mutant of T. denticola ATCC 35405 was constructed by allelic exchange mutation to investigate the role of tepA2 . .. Briefly, two fragments flanking the tepA2 gene were amplified with primer pairs 718D/719U and 719D/720U (listed in Table ), respectively.

    Article Title: Composition and Localization of Treponema denticola Outer Membrane Complexes ▿
    Article Snippet: .. T. denticola ATCC 35405 ( ) and isogenic msp mutant strain MHE ( ) were grown in NOS broth medium as previously described ( , ), with erythromycin (Em) (40 μg ml−1 ) added as appropriate. .. Cultures were examined by dark-field microscopy for purity and typical strain morphology.

    Sequencing:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Sequence homology-based screening The whole genome sequence of T. denticola ATCC 35405 in the Los Alamos oral pathogen database ( http://www.oralgen.org ) was screened for homologous sequences with the S. mutans bacteriocin immunity protein (ImmA/Bip) sequence [ ] using the protein blast program. .. The obtained homologous sequences were further compared against the database of National Center for Biotechnology Information (NCBI, http://blast.ncbi.nlm.nih.gov/Blast.cgi ).

    other:

    Article Title: The central region of the msp gene of Treponema denticola has sequence heterogeneity among clinical samples, obtained from patients with periodontitis
    Article Snippet: Based on these findings, the 17 samples were divided into 2 distinct groups, A (15 samples) and B (the remaining 2), whose msp nucleotide sequences were closely related to T. denticola ATCC 35405 and ATCC 33520, respectively.

    Construct:

    Article Title: Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
    Article Snippet: .. Construction of a tepA2 mutant As TepA2 showed similarity to ImmA, a tepA2- deficient mutant of T. denticola ATCC 35405 was constructed by allelic exchange mutation to investigate the role of tepA2 . .. Briefly, two fragments flanking the tepA2 gene were amplified with primer pairs 718D/719U and 719D/720U (listed in Table ), respectively.

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    ATCC t denticola atcc 35405
    Construction of T. <t>denticola</t> mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain <t>ATCC</t> 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC t denticola atcc 35404
    T. <t>denticola</t> proteases are not responsible for resistance to hβD-2. (A) T. denticola <t>ATCC</t> 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    T Denticola Atcc 35404, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Journal: Journal of microbiological methods

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

    doi: 10.1016/j.mimet.2010.07.020

    Figure Lengend Snippet: Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Article Snippet: T. denticola ATCC 35405 (the Type strain, which has been passaged extensively in various laboratories) was electroporated with the resulting linear DNA s and plated in NOS-GN soft agar medium containing erythromycin (40 μg ml−1 , Sigma Chemical Co., St. Louis, MO) as described previously.

    Techniques:

    Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Journal: Infection and Immunity

    Article Title: Cytopathic Effects of the Major Surface Protein and the Chymotrypsinlike Protease of Treponema denticola

    doi:

    Figure Lengend Snippet: Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Article Snippet: The objective of the present study was to quantify and compare the adherence activity and cytotoxic effects of two important outer membrane proteins of the T. denticola type strain ATCC 35405: the pore-forming adhesin Msp, and the chymotrypsinlike protease CTLP.

    Techniques: Transmission Assay, Sonication

    Histogram of fluorescence intensity of rhodamine-phalloidin-labeled F-actin in HGF during a 140-min time course, analyzed by confocal microscopy. There was significant depolymerization of F-actin in the T. denticola ATCC 35405-challenged HGF (left graph) in comparison with the control HGF (right graph). The degree of diminished fluorescence in T. denticola -challenged cells was significant at 60 min ( P

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Histogram of fluorescence intensity of rhodamine-phalloidin-labeled F-actin in HGF during a 140-min time course, analyzed by confocal microscopy. There was significant depolymerization of F-actin in the T. denticola ATCC 35405-challenged HGF (left graph) in comparison with the control HGF (right graph). The degree of diminished fluorescence in T. denticola -challenged cells was significant at 60 min ( P

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: Fluorescence, Labeling, Confocal Microscopy

    Comparison of filamentous actin disruption (mean ± standard deviation) in HGF challenged with different concentrations of whole cells of T. denticola ATCC 35405 (□) and OM extract (▪). The dry weight for the OM and the dry weight for the T. denticola cells were equivalent at each optical density (OD) point shown on the x axis.

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Comparison of filamentous actin disruption (mean ± standard deviation) in HGF challenged with different concentrations of whole cells of T. denticola ATCC 35405 (□) and OM extract (▪). The dry weight for the OM and the dry weight for the T. denticola cells were equivalent at each optical density (OD) point shown on the x axis.

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: Standard Deviation

    Qualitative comparison by SDS-PAGE of T. denticola ATCC 35405 whole cells (lane 1), OM extract run at the same time (lane 2), and repeat of the OM extract run in a different gel to resolve mid-range bands more clearly (lane 3). The positions of molecular size standards (broad-range SDS-PAGE standards; Bio-Rad) are shown in kilodaltons at the left. Conditions were as follows: Washed T. denticola suspensions (optical density of 1.2 at 550 nm) from 3-day cultures or undiluted OM extract were mixed 1:1 with sample buffer (0.25 M Tris-HCl [pH 6.8], 20% glycerol, 4.6% SDS, 0.02% bromophenol blue, 10% β-mercaptoethanol) and boiled for 10 min; 25 μl was added to each lane of a 1-mm-thick Ready Gel (Bio-Rad) with a 4% stacking gel and 12% resolving gel. Electrophoresis was run in a Mini-Protean II cell (Bio-Rad). Whole cells and OM extract had several bands in common.

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Qualitative comparison by SDS-PAGE of T. denticola ATCC 35405 whole cells (lane 1), OM extract run at the same time (lane 2), and repeat of the OM extract run in a different gel to resolve mid-range bands more clearly (lane 3). The positions of molecular size standards (broad-range SDS-PAGE standards; Bio-Rad) are shown in kilodaltons at the left. Conditions were as follows: Washed T. denticola suspensions (optical density of 1.2 at 550 nm) from 3-day cultures or undiluted OM extract were mixed 1:1 with sample buffer (0.25 M Tris-HCl [pH 6.8], 20% glycerol, 4.6% SDS, 0.02% bromophenol blue, 10% β-mercaptoethanol) and boiled for 10 min; 25 μl was added to each lane of a 1-mm-thick Ready Gel (Bio-Rad) with a 4% stacking gel and 12% resolving gel. Electrophoresis was run in a Mini-Protean II cell (Bio-Rad). Whole cells and OM extract had several bands in common.

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: SDS Page, Nucleic Acid Electrophoresis

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Journal: Infection and Immunity

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    doi: 10.1128/IAI.70.7.3982-3984.2002

    Figure Lengend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Article Snippet: As there was little difference in hβD-2 sensitivity among strains, T. denticola ATCC 35404 was used for all experiments.

    Techniques: Incubation