t denticola strain atcc 35404  (ATCC)


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    Name:
    Treponema denticola C CIP 103920
    Description:

    Catalog Number:
    35404
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    Structured Review

    ATCC t denticola strain atcc 35404
    CGase amino acid sequences from T. <t>denticola</t> strains <t>ATCC</t> 35404 and 35405. The top line in each row shows the predicted amino acid sequence for CGase from strain ATCC 35404 (this paper). The second line ). The asterisks represent amino acids that are identical in the two strains. Triangles ).

    https://www.bioz.com/result/t denticola strain atcc 35404/product/ATCC
    Average 94 stars, based on 1 article reviews
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    t denticola strain atcc 35404 - by Bioz Stars, 2020-08
    94/100 stars

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    Images

    1) Product Images from "A 52-kDa Leucyl Aminopeptidase from Treponema denticola Is a Cysteinylglycinase That Mediates the Second Step of Glutathione Metabolism *"

    Article Title: A 52-kDa Leucyl Aminopeptidase from Treponema denticola Is a Cysteinylglycinase That Mediates the Second Step of Glutathione Metabolism *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M801034200

    CGase amino acid sequences from T. denticola strains ATCC 35404 and 35405. The top line in each row shows the predicted amino acid sequence for CGase from strain ATCC 35404 (this paper). The second line ). The asterisks represent amino acids that are identical in the two strains. Triangles ).
    Figure Legend Snippet: CGase amino acid sequences from T. denticola strains ATCC 35404 and 35405. The top line in each row shows the predicted amino acid sequence for CGase from strain ATCC 35404 (this paper). The second line ). The asterisks represent amino acids that are identical in the two strains. Triangles ).

    Techniques Used: Sequencing

    Fractionation of 52-kDa CGase from T. denticola ATCC 35404 on a HiTrap Q FF column. a , fractions from the separation of a partially purified protein extract on a HiTrap Sepharose Q FF ion exchange column were assayed for CGase activity ( squares ) and total protein ( diamonds , as OD 280 ). b ) of the proteins present in the sample that was loaded onto the ion exchange column ( lane 1 ) and the proteins present in fraction 15 that had the peak CGase activity ( lane 2 ). Proteins of known molecular weight were run in lane MW , and the gel was stained with 0.025% Coomassie Brilliant Blue R-250.
    Figure Legend Snippet: Fractionation of 52-kDa CGase from T. denticola ATCC 35404 on a HiTrap Q FF column. a , fractions from the separation of a partially purified protein extract on a HiTrap Sepharose Q FF ion exchange column were assayed for CGase activity ( squares ) and total protein ( diamonds , as OD 280 ). b ) of the proteins present in the sample that was loaded onto the ion exchange column ( lane 1 ) and the proteins present in fraction 15 that had the peak CGase activity ( lane 2 ). Proteins of known molecular weight were run in lane MW , and the gel was stained with 0.025% Coomassie Brilliant Blue R-250.

    Techniques Used: Fractionation, Purification, Activity Assay, Molecular Weight, Staining

    Related Articles

    Incubation:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. To determine the ability of T. denticola to replicate following exposure to hβD-2, T. denticola ATCC 35404 cultures at 107 organisms/ml were incubated with and without 10 μg of hβD-2 per ml in GM-1 medium at 37°C and 5% CO2 for 4 h. Cultures were diluted serially in semisolid GM-1 medium (with 0.5% Noble agar and 0.5% gelatin) and incubated anaerobically at 37°C for 1 to 2 weeks ( ). .. E. coli incubated in GM-1 medium was used as a control for hβD-2 activity and was quantitated by serial dilution on LB agar.

    Metabolic Labelling:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. Results from four experiments confirm that T. denticola ATCC 35404 remains metabolically active after incubation with hβD-2. .. In a representative experiment, incubation in medium alone showed 72.06% ± 10.01% (mean ± standard error of the mean [SEM]) reduction, while incubation with 10 μg of hβD-2 per ml resulted in 71.57% ± 9.88% reduction.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Lack of Humoral Immune Protection against Treponema denticola Virulence in a Murine Model
    Article Snippet: .. T. denticola ATCC 35404 soluble antigen polypeptides were separated by SDS–10% PAGE. .. The proteins were electrophoretically transferred onto nitrocellulose paper (0.25-μm pore size; Schleicher & Schuell, Inc., Keene, N.H.) by using 25 mM Tris–192 mM glycine–20% methanol buffer (pH 8.3) at 8 V/cm for 3 h at 4°C ( ).

    Microscopy:

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins
    Article Snippet: .. T. denticola ATCC 35404 and 10 μg of hβD-2 per ml were incubated in the presence or absence of final concentrations of 100 μM chymostatin (Sigma Chemicals, St. Louis, Mo.) at 37°C and 5% CO2 for 4 h. Viable bacteria were enumerated by dark-field microscopy ( T. denticola ) or plate counts ( E. coli ). ..

    Purification:

    Article Title: Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
    Article Snippet: .. Chu L, Holt S C. Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404. ..

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    ATCC t denticola atcc 35405
    Construction of T. <t>denticola</t> mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain <t>ATCC</t> 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .
    T Denticola Atcc 35405, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC t denticola atcc 35404
    T. <t>denticola</t> proteases are not responsible for resistance to hβD-2. (A) T. denticola <t>ATCC</t> 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.
    T Denticola Atcc 35404, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t denticola atcc 35404/product/ATCC
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    t denticola atcc 35404 - by Bioz Stars, 2020-08
    94/100 stars
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    Image Search Results


    Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Journal: Journal of microbiological methods

    Article Title: A simplified erythromycin resistance cassette for Treponema denticola mutagenesis

    doi: 10.1016/j.mimet.2010.07.020

    Figure Lengend Snippet: Construction of T. denticola mutants using ermF-ermB and ermB cassettes. The protease operon ( prcB-prcA-prtP ) and adjacent gene TDE0759 in wildtype parent strain ATCC 35405 and isogenic strains mutated in prcB (P0760 and CF548) and prcA (PNE and CF547) showing locations of ermF-ermB and ermB insertions. The symbol x- indicates gene disruption in prcA or prcB .

    Article Snippet: T. denticola ATCC 35405 (the Type strain, which has been passaged extensively in various laboratories) was electroporated with the resulting linear DNA s and plated in NOS-GN soft agar medium containing erythromycin (40 μg ml−1 , Sigma Chemical Co., St. Louis, MO) as described previously.

    Techniques:

    Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Journal: Infection and Immunity

    Article Title: Cytopathic Effects of the Major Surface Protein and the Chymotrypsinlike Protease of Treponema denticola

    doi:

    Figure Lengend Snippet: Transmission electron micrograph of T. denticola ATCC 35405 outer membrane material released by mild sonication and probed with antibodies directed against Msp (10-nm gold beads) and CTLP (5-nm gold beads). Bar, 0.3 μm.

    Article Snippet: The objective of the present study was to quantify and compare the adherence activity and cytotoxic effects of two important outer membrane proteins of the T. denticola type strain ATCC 35405: the pore-forming adhesin Msp, and the chymotrypsinlike protease CTLP.

    Techniques: Transmission Assay, Sonication

    Histogram of fluorescence intensity of rhodamine-phalloidin-labeled F-actin in HGF during a 140-min time course, analyzed by confocal microscopy. There was significant depolymerization of F-actin in the T. denticola ATCC 35405-challenged HGF (left graph) in comparison with the control HGF (right graph). The degree of diminished fluorescence in T. denticola -challenged cells was significant at 60 min ( P

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Histogram of fluorescence intensity of rhodamine-phalloidin-labeled F-actin in HGF during a 140-min time course, analyzed by confocal microscopy. There was significant depolymerization of F-actin in the T. denticola ATCC 35405-challenged HGF (left graph) in comparison with the control HGF (right graph). The degree of diminished fluorescence in T. denticola -challenged cells was significant at 60 min ( P

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: Fluorescence, Labeling, Confocal Microscopy

    Comparison of filamentous actin disruption (mean ± standard deviation) in HGF challenged with different concentrations of whole cells of T. denticola ATCC 35405 (□) and OM extract (▪). The dry weight for the OM and the dry weight for the T. denticola cells were equivalent at each optical density (OD) point shown on the x axis.

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Comparison of filamentous actin disruption (mean ± standard deviation) in HGF challenged with different concentrations of whole cells of T. denticola ATCC 35405 (□) and OM extract (▪). The dry weight for the OM and the dry weight for the T. denticola cells were equivalent at each optical density (OD) point shown on the x axis.

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: Standard Deviation

    Qualitative comparison by SDS-PAGE of T. denticola ATCC 35405 whole cells (lane 1), OM extract run at the same time (lane 2), and repeat of the OM extract run in a different gel to resolve mid-range bands more clearly (lane 3). The positions of molecular size standards (broad-range SDS-PAGE standards; Bio-Rad) are shown in kilodaltons at the left. Conditions were as follows: Washed T. denticola suspensions (optical density of 1.2 at 550 nm) from 3-day cultures or undiluted OM extract were mixed 1:1 with sample buffer (0.25 M Tris-HCl [pH 6.8], 20% glycerol, 4.6% SDS, 0.02% bromophenol blue, 10% β-mercaptoethanol) and boiled for 10 min; 25 μl was added to each lane of a 1-mm-thick Ready Gel (Bio-Rad) with a 4% stacking gel and 12% resolving gel. Electrophoresis was run in a Mini-Protean II cell (Bio-Rad). Whole cells and OM extract had several bands in common.

    Journal: Infection and Immunity

    Article Title: Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola

    doi:

    Figure Lengend Snippet: Qualitative comparison by SDS-PAGE of T. denticola ATCC 35405 whole cells (lane 1), OM extract run at the same time (lane 2), and repeat of the OM extract run in a different gel to resolve mid-range bands more clearly (lane 3). The positions of molecular size standards (broad-range SDS-PAGE standards; Bio-Rad) are shown in kilodaltons at the left. Conditions were as follows: Washed T. denticola suspensions (optical density of 1.2 at 550 nm) from 3-day cultures or undiluted OM extract were mixed 1:1 with sample buffer (0.25 M Tris-HCl [pH 6.8], 20% glycerol, 4.6% SDS, 0.02% bromophenol blue, 10% β-mercaptoethanol) and boiled for 10 min; 25 μl was added to each lane of a 1-mm-thick Ready Gel (Bio-Rad) with a 4% stacking gel and 12% resolving gel. Electrophoresis was run in a Mini-Protean II cell (Bio-Rad). Whole cells and OM extract had several bands in common.

    Article Snippet: Stock cultures of T. denticola ATCC 35405 (type strain), originally provided by W. J. Loesche, University of Michigan, and ATCC 35404, ATCC 33520, e, and e′, provided by E. C. S. Chan, McGill University, were maintained and grown for experiments in a complex spirochete broth medium containing brain heart infusion, tryptic peptone, yeast extract, and volatile fatty acids and supplemented with 2.0% rabbit serum as previously described ( ).

    Techniques: SDS Page, Nucleic Acid Electrophoresis

    T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Journal: Infection and Immunity

    Article Title: Treponema denticola Is Resistant to Human ?-Defensins

    doi: 10.1128/IAI.70.7.3982-3984.2002

    Figure Lengend Snippet: T. denticola proteases are not responsible for resistance to hβD-2. (A) T. denticola ATCC 35404 incubated in the presence or absence of 100 μM chymostatin (CHY) remained resistant to 10 μg of hβD-2 per ml over a 4-h period. (B) E. coli was incubated in the presence of 10 μg of hβD-2 per ml for 4 h, with or without equal numbers of T. denticola ATCC 35404. Data are means ± SEM from five (A) and three (B) experiments. SDS, sodium dodecyl sulfate.

    Article Snippet: As there was little difference in hβD-2 sensitivity among strains, T. denticola ATCC 35404 was used for all experiments.

    Techniques: Incubation